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The map label for this gene is cspA [C]

Identifier: 192289774

GI number: 192289774

Start: 1437358

End: 1437573

Strand: Reverse

Name: cspA [C]

Synonym: Rpal_1364

Alternate gene names: 192289774

Gene position: 1437573-1437358 (Counterclockwise)

Preceding gene: 192289775

Following gene: 192289772

Centisome position: 25.03

GC content: 56.48

Gene sequence:

>216_bases
ATGGCAATGACGGGAACGGTGAAGTTCTTCAACGGCGAGCGCGGCTACGGCTTCATCAAGCCTGATGACGGTGGCCGCGA
TGTGTTCGTACACATTACCGCAGTCGAACGGGCCGGCTTGAAAGACTTGACCGAGGGACAGCGTATTTCATTCGAAGTGG
AGCCTGACAAGAAGGGCAAAGGCCCCAAGGCGGTCAACTTGGTGATCTCGCCGTAA

Upstream 100 bases:

>100_bases
TAGCAGGTCCCGCTCTTGGCCAAGCTATCCGGCAGCGAAATGCCAGGTCGCACCGATGCGCCGGCGCGCGTCGGAAAGCA
TGCTGGGAGGAGTTTCCGGA

Downstream 100 bases:

>100_bases
TCGGCTCGGAAAGCTGCAGCGTCGCGGCGGCGCTGCACGGGTTGGGACGGGGCGCGATAACGTTGGCGCGTGCCATTAGG
TAGCGCGCAGTGAGTTCGAG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 71; Mature: 70

Protein sequence:

>71_residues
MAMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGKGPKAVNLVISP

Sequences:

>Translated_71_residues
MAMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGKGPKAVNLVISP
>Mature_70_residues
AMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGKGPKAVNLVISP

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=66, Percent_Identity=53.030303030303, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=47.0588235294118, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=48.4848484848485, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786841, Length=66, Percent_Identity=48.4848484848485, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=51.5151515151515, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=49.2307692307692, Blast_Score=70, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7724; Mature: 7593

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGK
CCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCC
GPKAVNLVISP
CCEEEEEEEEC
>Mature Secondary Structure 
AMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLTEGQRISFEVEPDKKGK
CCCCEEEEECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCC
GPKAVNLVISP
CCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]