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The map label for this gene is pgk [H]

Identifier: 192289440

GI number: 192289440

Start: 1074795

End: 1075991

Strand: Reverse

Name: pgk [H]

Synonym: Rpal_1013

Alternate gene names: 192289440

Gene position: 1075991-1074795 (Counterclockwise)

Preceding gene: 192289441

Following gene: 192289439

Centisome position: 18.73

GC content: 67.5

Gene sequence:

>1197_bases
ATGACAAAGACATTCCGCACCCTCGACGACGTCGATGTGAAGGGCAAGCGCGTGCTGCTGCGCGTCGATCTCAACGTGCC
GATGGACGCCGGCAAGGTGACCGACACCACCCGGCTCGAACGCGTGATGCCGACCATCACCGAGCTGTCGGGCAAGGGCG
CCAAGGTGATCCTGCTGGCGCATTTCGGCCGGCCGAAGGGCCGCGACGACAAGAACTCGCTCAAGCCGGTCGCTGCTGCG
CTGGCCGACGTCATCAAGAAGCCGGTCGGCTTCGCTGACGACTGCATCGGCGAGCCCGCCGCCAAGGCGATCGCCGCGAT
GGCCGACGGCGACATCCTGTGCCTGGAAAACACCCGCTTCCATCCCGAGGAAGAGAAGAACGATCCGGCGTTCGTCGCCA
AGCTCGCCGAGCTCGGCGACATCTGGGTCAACGACGCGTTCTCGGCCGCGCACCGCGCCCACGCCACCACCGAAGGCCTC
GGCCACAAGCTGCCGGCTTACGCGGGCCGCACCATGCAGGCCGAGCTCGTCGCGCTGAACAAGGCGCTGGAAGCGCCGGT
GAAGCCGGTGATCGCGATCGTCGGCGGCGCCAAGGTGTCGACCAAGATCGACCTGCTCGAAAACCTGGTCACCAAGGTGC
AGGCGCTGGTGATCGGCGGCGGCATGGCCAACACCTTCCTGCACGCCCAGGGCGTCAAGGTCGGCAAGTCGCTGTGCGAG
AAGGACCTGGCCCCGACCGCGCTGCGCATCCTCGATAAGGCCGAAGCTGCCAACTGCGCCATCATCCTGCCGGTCGACGC
CACCGTGGCCTATCACTTCGAGGCCAATGCGCCGTCGTTCGCCTATGGGCTCGACGCCATCCCGGACGATGCCATGATCC
TCGACGTCGGCCCGCGTTCGATCGAGCGCATTCACGCCGCGATCGACGATGCCGCGACCCTGGTGTGGAACGGCCCGGTC
GGCGCGTTCGAGCTGACGCCGTTCGACAAGGGCACGGTCGAAGCCGCCAAGCATGCCGCCAAGCGCACCAGGGCCGGCAA
GCTGGTGTCGGTCGCCGGCGGCGGCGACACCGTCGCGGCGCTCAACCATGCCGGCGTGGCCGACGACTTCACCTACATCT
CGACCGCCGGCGGCGCCTTCCTGGAATGGATGGAAGGCAAGCCGCTGCCGGGCGTGGAAGTGCTCCAGGTCAAGTGA

Upstream 100 bases:

>100_bases
GCCGAAGCTGTTCTTCGTGCGCCCCTCATCCTCCCTCATCCTGAGGAGCCGAGCGCAGCGAGGCGTCTCGAAGGATGACA
CCCAAGGCACCGGGTTCATC

Downstream 100 bases:

>100_bases
TCCCTACTGCCGGCGCCCCCCGCGGCGCCGGCTTGCGACCGCCTCCACCGGAGCCTATCTTGCGTTACATGTAACGCTCC
GGTCGGAGGAGGCCTGCAAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTKTFRTLDDVDVKGKRVLLRVDLNVPMDAGKVTDTTRLERVMPTITELSGKGAKVILLAHFGRPKGRDDKNSLKPVAAA
LADVIKKPVGFADDCIGEPAAKAIAAMADGDILCLENTRFHPEEEKNDPAFVAKLAELGDIWVNDAFSAAHRAHATTEGL
GHKLPAYAGRTMQAELVALNKALEAPVKPVIAIVGGAKVSTKIDLLENLVTKVQALVIGGGMANTFLHAQGVKVGKSLCE
KDLAPTALRILDKAEAANCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRSIERIHAAIDDAATLVWNGPV
GAFELTPFDKGTVEAAKHAAKRTRAGKLVSVAGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLQVK

Sequences:

>Translated_398_residues
MTKTFRTLDDVDVKGKRVLLRVDLNVPMDAGKVTDTTRLERVMPTITELSGKGAKVILLAHFGRPKGRDDKNSLKPVAAA
LADVIKKPVGFADDCIGEPAAKAIAAMADGDILCLENTRFHPEEEKNDPAFVAKLAELGDIWVNDAFSAAHRAHATTEGL
GHKLPAYAGRTMQAELVALNKALEAPVKPVIAIVGGAKVSTKIDLLENLVTKVQALVIGGGMANTFLHAQGVKVGKSLCE
KDLAPTALRILDKAEAANCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRSIERIHAAIDDAATLVWNGPV
GAFELTPFDKGTVEAAKHAAKRTRAGKLVSVAGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLQVK
>Mature_397_residues
TKTFRTLDDVDVKGKRVLLRVDLNVPMDAGKVTDTTRLERVMPTITELSGKGAKVILLAHFGRPKGRDDKNSLKPVAAAL
ADVIKKPVGFADDCIGEPAAKAIAAMADGDILCLENTRFHPEEEKNDPAFVAKLAELGDIWVNDAFSAAHRAHATTEGLG
HKLPAYAGRTMQAELVALNKALEAPVKPVIAIVGGAKVSTKIDLLENLVTKVQALVIGGGMANTFLHAQGVKVGKSLCEK
DLAPTALRILDKAEAANCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRSIERIHAAIDDAATLVWNGPVG
AFELTPFDKGTVEAAKHAAKRTRAGKLVSVAGGGDTVAALNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLQVK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=43.0656934306569, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=42.5790754257908, Blast_Score=282, Evalue=4e-76,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=43.6224489795918, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17508823, Length=408, Percent_Identity=45.0980392156863, Blast_Score=311, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=40.3892944038929, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=42.1951219512195, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=41.421568627451, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41913; Mature: 41781

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTFRTLDDVDVKGKRVLLRVDLNVPMDAGKVTDTTRLERVMPTITELSGKGAKVILLA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
HFGRPKGRDDKNSLKPVAAALADVIKKPVGFADDCIGEPAAKAIAAMADGDILCLENTRF
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCEEEEECCCC
HPEEEKNDPAFVAKLAELGDIWVNDAFSAAHRAHATTEGLGHKLPAYAGRTMQAELVALN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHH
KALEAPVKPVIAIVGGAKVSTKIDLLENLVTKVQALVIGGGMANTFLHAQGVKVGKSLCE
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KDLAPTALRILDKAEAANCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRS
HCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCEECCCCCCCCCEEEECCHHH
IERIHAAIDDAATLVWNGPVGAFELTPFDKGTVEAAKHAAKRTRAGKLVSVAGGGDTVAA
HHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEE
LNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLQVK
ECCCCCCCCCCEEECCCCHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure 
TKTFRTLDDVDVKGKRVLLRVDLNVPMDAGKVTDTTRLERVMPTITELSGKGAKVILLA
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
HFGRPKGRDDKNSLKPVAAALADVIKKPVGFADDCIGEPAAKAIAAMADGDILCLENTRF
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCEEEEECCCC
HPEEEKNDPAFVAKLAELGDIWVNDAFSAAHRAHATTEGLGHKLPAYAGRTMQAELVALN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHH
KALEAPVKPVIAIVGGAKVSTKIDLLENLVTKVQALVIGGGMANTFLHAQGVKVGKSLCE
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KDLAPTALRILDKAEAANCAIILPVDATVAYHFEANAPSFAYGLDAIPDDAMILDVGPRS
HCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCEECCCCCCCCCEEEECCHHH
IERIHAAIDDAATLVWNGPVGAFELTPFDKGTVEAAKHAAKRTRAGKLVSVAGGGDTVAA
HHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEE
LNHAGVADDFTYISTAGGAFLEWMEGKPLPGVEVLQVK
ECCCCCCCCCCEEECCCCHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA