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The map label for this gene is mltB [H]

Identifier: 192289396

GI number: 192289396

Start: 1035230

End: 1036615

Strand: Reverse

Name: mltB [H]

Synonym: Rpal_0968

Alternate gene names: 192289396

Gene position: 1036615-1035230 (Counterclockwise)

Preceding gene: 192289398

Following gene: 192289395

Centisome position: 18.05

GC content: 68.04

Gene sequence:

>1386_bases
ATGACGGGACACAACCGGCTGGCAGCCGCCGCGCTCGCGGCATGGTTCGGCGGCGTGGCGCTTTCGGCCACGGCGGCGCA
GGCGCAATCCGGCAATCCTCTGAACTTCCTCGACAGCATTTTCGGCAATCCGTCGCCGAGCCGCCCGGCTGCAACCGGCG
CTGGCGCGCCCTCAGGCGGTAGCTCCGCCGTGCAGCCATGGAGCGGCGAGGACGGGGCTTCGGGCCATCCGCTGATGACC
GCGGCGGCAATCCGCGAGGCCGCGGCGAACTTCGACAATTGCGTCGCCGCGATGTGGCCGGACGCGGCCCGCCGCGGCAT
CTCCCGCGAGTCATTCGAGCGCTACACCGCCGGCCTGTCGCCCGACCTGCGGCTGATGGACCTGATGGATTCGCAGCCGG
AGTTCACCAAATCGATCTGGGATTATCTCGACATCCTGGTCAACGACACACGCCTCGCCAAGGGCCGCGAAATCCTGGCG
CAGTACAAGCCGCAGTTCGATGCGGTCGAGCGCGCCTACGGCGTCGACCGCTACATCATCGCATCGATCTGGGGAATCGA
ATCGAACTATTCGACCCAGATGGGCGATCGCTATGTGGTGAACTCGACCGCGACGCTGGCTTGCGTCGGCCGCCGCCAGG
CGTACTTCAAGGACGAATTCCTCACCGCGCTGGAGATCCTGCATCGCGGCGACCTGCGTCCCGAGCAGCTGCGCGGCTCA
TGGGCCGGCGCATTCGGGCCGACGCAATTCATGCCGACCGCGTTCAAGCGCTTCGCGGTCGACGCCGACGGCGACGGCCG
CCGCGACGTCGTCGACAATCCCTACGACCTGATCGCGTCGACCGCCAACAACCTGAAGAAGGACGGCTGGCAGACCGGCC
AGACCTGGGGCTACGAGGTCGTGGTGCCCCGCGGCTTCAACTACATGCTGGCCGACCGCCGCCAGACCCAGACTCTCGCG
CAGTGGGAGCAGATGGGCCTGCGCCGCGCGGGCGGCCAGCCGTTCCCGCGCTCGAACGAAAAGGCCTACCTGCTCGCCCC
CGCCGGCGCCGCAGGTCCCGGCTTCCTGATGCTCACCAATTTCCGGGTGATCATGAAGTACAACCCGGCTGAGGCCTACG
CGCTGGCGATCGGCCATTTCGCCGACCGGCTGCGCGGCGGCGCCCCGTTCGTCCAGGAGTGGCCGCGCCAGGAGCGCGCC
CTGTCCCGCACCGAGAAGCTGGAATTGCAACAGCTCCTCGCCCAGCGCGGCTTCTACCGCGGCACCCCCGACGGCCAGTT
CGGCTCGGAAACCCGCAGCGCACTGCGGAATTTCCAGGCCTCGATCGGCGCCCCGGCGGATGGATTTGCGACCGGGGAAA
TGTTGGAGAGGTTGAGAGGTCGCTGA

Upstream 100 bases:

>100_bases
GGCGACCGGACGGCAAAAAGCCGCTTTGTTAAGCTGATGGAAACCATCACGGCGCCTCCTGCCCCCCGGGGGCCTCGCAA
CAGGTGGGATGCAATCGACG

Downstream 100 bases:

>100_bases
GTTCAGCGCCTGCCACAAACACCACGTCTGACGAGTGATTACTGCTGCGCGCTCGGTGTCGGCGCCGCCCCCGTTGTCGT
CGTCGCTCCTCCCGCCTGCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTGHNRLAAAALAAWFGGVALSATAAQAQSGNPLNFLDSIFGNPSPSRPAATGAGAPSGGSSAVQPWSGEDGASGHPLMT
AAAIREAAANFDNCVAAMWPDAARRGISRESFERYTAGLSPDLRLMDLMDSQPEFTKSIWDYLDILVNDTRLAKGREILA
QYKPQFDAVERAYGVDRYIIASIWGIESNYSTQMGDRYVVNSTATLACVGRRQAYFKDEFLTALEILHRGDLRPEQLRGS
WAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPYDLIASTANNLKKDGWQTGQTWGYEVVVPRGFNYMLADRRQTQTLA
QWEQMGLRRAGGQPFPRSNEKAYLLAPAGAAGPGFLMLTNFRVIMKYNPAEAYALAIGHFADRLRGGAPFVQEWPRQERA
LSRTEKLELQQLLAQRGFYRGTPDGQFGSETRSALRNFQASIGAPADGFATGEMLERLRGR

Sequences:

>Translated_461_residues
MTGHNRLAAAALAAWFGGVALSATAAQAQSGNPLNFLDSIFGNPSPSRPAATGAGAPSGGSSAVQPWSGEDGASGHPLMT
AAAIREAAANFDNCVAAMWPDAARRGISRESFERYTAGLSPDLRLMDLMDSQPEFTKSIWDYLDILVNDTRLAKGREILA
QYKPQFDAVERAYGVDRYIIASIWGIESNYSTQMGDRYVVNSTATLACVGRRQAYFKDEFLTALEILHRGDLRPEQLRGS
WAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPYDLIASTANNLKKDGWQTGQTWGYEVVVPRGFNYMLADRRQTQTLA
QWEQMGLRRAGGQPFPRSNEKAYLLAPAGAAGPGFLMLTNFRVIMKYNPAEAYALAIGHFADRLRGGAPFVQEWPRQERA
LSRTEKLELQQLLAQRGFYRGTPDGQFGSETRSALRNFQASIGAPADGFATGEMLERLRGR
>Mature_460_residues
TGHNRLAAAALAAWFGGVALSATAAQAQSGNPLNFLDSIFGNPSPSRPAATGAGAPSGGSSAVQPWSGEDGASGHPLMTA
AAIREAAANFDNCVAAMWPDAARRGISRESFERYTAGLSPDLRLMDLMDSQPEFTKSIWDYLDILVNDTRLAKGREILAQ
YKPQFDAVERAYGVDRYIIASIWGIESNYSTQMGDRYVVNSTATLACVGRRQAYFKDEFLTALEILHRGDLRPEQLRGSW
AGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPYDLIASTANNLKKDGWQTGQTWGYEVVVPRGFNYMLADRRQTQTLAQ
WEQMGLRRAGGQPFPRSNEKAYLLAPAGAAGPGFLMLTNFRVIMKYNPAEAYALAIGHFADRLRGGAPFVQEWPRQERAL
SRTEKLELQQLLAQRGFYRGTPDGQFGSETRSALRNFQASIGAPADGFATGEMLERLRGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=289, Percent_Identity=28.3737024221453, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 50477; Mature: 50346

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHNRLAAAALAAWFGGVALSATAAQAQSGNPLNFLDSIFGNPSPSRPAATGAGAPSGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
SSAVQPWSGEDGASGHPLMTAAAIREAAANFDNCVAAMWPDAARRGISRESFERYTAGLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCHHHHHHHHCCCC
PDLRLMDLMDSQPEFTKSIWDYLDILVNDTRLAKGREILAQYKPQFDAVERAYGVDRYII
CCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
ASIWGIESNYSTQMGDRYVVNSTATLACVGRRQAYFKDEFLTALEILHRGDLRPEQLRGS
HHHHCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
WAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPYDLIASTANNLKKDGWQTGQTWGYEV
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
VVPRGFNYMLADRRQTQTLAQWEQMGLRRAGGQPFPRSNEKAYLLAPAGAAGPGFLMLTN
EECCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEC
FRVIMKYNPAEAYALAIGHFADRLRGGAPFVQEWPRQERALSRTEKLELQQLLAQRGFYR
EEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
GTPDGQFGSETRSALRNFQASIGAPADGFATGEMLERLRGR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TGHNRLAAAALAAWFGGVALSATAAQAQSGNPLNFLDSIFGNPSPSRPAATGAGAPSGG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCC
SSAVQPWSGEDGASGHPLMTAAAIREAAANFDNCVAAMWPDAARRGISRESFERYTAGLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCHHHHHHHHCCCC
PDLRLMDLMDSQPEFTKSIWDYLDILVNDTRLAKGREILAQYKPQFDAVERAYGVDRYII
CCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
ASIWGIESNYSTQMGDRYVVNSTATLACVGRRQAYFKDEFLTALEILHRGDLRPEQLRGS
HHHHCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
WAGAFGPTQFMPTAFKRFAVDADGDGRRDVVDNPYDLIASTANNLKKDGWQTGQTWGYEV
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
VVPRGFNYMLADRRQTQTLAQWEQMGLRRAGGQPFPRSNEKAYLLAPAGAAGPGFLMLTN
EECCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEEC
FRVIMKYNPAEAYALAIGHFADRLRGGAPFVQEWPRQERALSRTEKLELQQLLAQRGFYR
EEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
GTPDGQFGSETRSALRNFQASIGAPADGFATGEMLERLRGR
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]