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The map label for this gene is cysW [H]

Identifier: 192289247

GI number: 192289247

Start: 863372

End: 864286

Strand: Reverse

Name: cysW [H]

Synonym: Rpal_0819

Alternate gene names: 192289247

Gene position: 864286-863372 (Counterclockwise)

Preceding gene: 192289248

Following gene: 192289246

Centisome position: 15.05

GC content: 64.26

Gene sequence:

>915_bases
ATGACGATGGTTGCCACCAGTCAAGTCAGGCGTGCAGCGATCGCATCTGCCGGCGCCGCCGCACCAGCCGCCGCCGGGCG
CCGTTCCGCCCACACCGAGCCGGCTTGGGTGCGGCTGCTGATCATCGGATCCGCTCTCACCTTCCTCACCGTGTTCGTAG
TGCTGCCGCTGATCCTGGTGTTTTCCGAGGCCCTGGCGAAGGGCGCAGCGTTCTATCTGTCGGCGCTGGCCGACAACGAA
GCGCTTGCGGCGATCAAGCTGACGCTGATTGCGGCCGGCATTTCGGTCGGCCTCAATCTGGTATTCGGTGTCATCGCCGC
CTGGGCGATTGCGAAATTCGACTTTCCGGGCAAGACGTTCCTAATCACGCTGATCGATCTACCGTTCTCGGTCTCTCCCG
TGATCTCGGGCCTCGTGTTCGTGCTGCTGTTCGGCGCGCAAGGCTGGTTCGGGCCGTGGCTGATGGCGCACGGCGTCCAG
ATCCTGTTCGCGGTGCCGGCGATTGTGCTGGCGACGACCTTCGTCACCTTCCCGTTCGTGGCGCGCGAACTGATCCCGCT
GATGCAGGAGCAGGGCCAGCAGGAAGAGGAAGCCGCGATCTCGCTCGGCGCCAGTGGCTGGACCACGTTCTGGCGGGTCA
CGATGCCGAACATCAAATGGGGCCTGCTGTACGGCGTACTGCTGTGCAACGCCCGCGCGATGGGCGAATTCGGCGCGGTG
TCGGTGGTGTCGGGCCATATCCGCGGCGAGACCAACACCATGCCGCTGCTGGTGGAAATTCTGTACAACGAGTACCAGAT
GGTGGCCGCATTCGCGATCGCCTCGCTGCTGGCGCTGCTCGCGCTGGTGACGCTGATCGCTAAGACCGTGCTGGAAGGCC
GCATCGAAGAGGGGCTGCATCCCGATGACCATTGA

Upstream 100 bases:

>100_bases
ATCGCGGTGGTGATGCTGATCTTCTCGTTCCTGATCATCTTCGTCCTCAATCGGATCCAGCACTGGGCGCAATCCCGGTC
GCTGGCCACGGCGTGACGCC

Downstream 100 bases:

>100_bases
AGTCCGCAACATCGTCAAGGAATTCGGCAGCTTCCGAGCACTCGACAATGTCGATCTGCGGGTCGAGACCGGCGAACTGA
TGGCGCTGCTCGGTCCGTCC

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTMVATSQVRRAAIASAGAAAPAAAGRRSAHTEPAWVRLLIIGSALTFLTVFVVLPLILVFSEALAKGAAFYLSALADNE
ALAAIKLTLIAAGISVGLNLVFGVIAAWAIAKFDFPGKTFLITLIDLPFSVSPVISGLVFVLLFGAQGWFGPWLMAHGVQ
ILFAVPAIVLATTFVTFPFVARELIPLMQEQGQQEEEAAISLGASGWTTFWRVTMPNIKWGLLYGVLLCNARAMGEFGAV
SVVSGHIRGETNTMPLLVEILYNEYQMVAAFAIASLLALLALVTLIAKTVLEGRIEEGLHPDDH

Sequences:

>Translated_304_residues
MTMVATSQVRRAAIASAGAAAPAAAGRRSAHTEPAWVRLLIIGSALTFLTVFVVLPLILVFSEALAKGAAFYLSALADNE
ALAAIKLTLIAAGISVGLNLVFGVIAAWAIAKFDFPGKTFLITLIDLPFSVSPVISGLVFVLLFGAQGWFGPWLMAHGVQ
ILFAVPAIVLATTFVTFPFVARELIPLMQEQGQQEEEAAISLGASGWTTFWRVTMPNIKWGLLYGVLLCNARAMGEFGAV
SVVSGHIRGETNTMPLLVEILYNEYQMVAAFAIASLLALLALVTLIAKTVLEGRIEEGLHPDDH
>Mature_303_residues
TMVATSQVRRAAIASAGAAAPAAAGRRSAHTEPAWVRLLIIGSALTFLTVFVVLPLILVFSEALAKGAAFYLSALADNEA
LAAIKLTLIAAGISVGLNLVFGVIAAWAIAKFDFPGKTFLITLIDLPFSVSPVISGLVFVLLFGAQGWFGPWLMAHGVQI
LFAVPAIVLATTFVTFPFVARELIPLMQEQGQQEEEAAISLGASGWTTFWRVTMPNIKWGLLYGVLLCNARAMGEFGAVS
VVSGHIRGETNTMPLLVEILYNEYQMVAAFAIASLLALLALVTLIAKTVLEGRIEEGLHPDDH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=271, Percent_Identity=50.5535055350554, Blast_Score=277, Evalue=6e-76,
Organism=Escherichia coli, GI1788764, Length=223, Percent_Identity=34.9775784753363, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=33.1491712707182, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32350; Mature: 32218

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMVATSQVRRAAIASAGAAAPAAAGRRSAHTEPAWVRLLIIGSALTFLTVFVVLPLILV
CCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSEALAKGAAFYLSALADNEALAAIKLTLIAAGISVGLNLVFGVIAAWAIAKFDFPGKTF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LITLIDLPFSVSPVISGLVFVLLFGAQGWFGPWLMAHGVQILFAVPAIVLATTFVTFPFV
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARELIPLMQEQGQQEEEAAISLGASGWTTFWRVTMPNIKWGLLYGVLLCNARAMGEFGAV
HHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCH
SVVSGHIRGETNTMPLLVEILYNEYQMVAAFAIASLLALLALVTLIAKTVLEGRIEEGLH
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDDH
CCCC
>Mature Secondary Structure 
TMVATSQVRRAAIASAGAAAPAAAGRRSAHTEPAWVRLLIIGSALTFLTVFVVLPLILV
CCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSEALAKGAAFYLSALADNEALAAIKLTLIAAGISVGLNLVFGVIAAWAIAKFDFPGKTF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LITLIDLPFSVSPVISGLVFVLLFGAQGWFGPWLMAHGVQILFAVPAIVLATTFVTFPFV
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARELIPLMQEQGQQEEEAAISLGASGWTTFWRVTMPNIKWGLLYGVLLCNARAMGEFGAV
HHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCH
SVVSGHIRGETNTMPLLVEILYNEYQMVAAFAIASLLALLALVTLIAKTVLEGRIEEGLH
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDDH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]