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The map label for this gene is ispA [C]

Identifier: 192288955

GI number: 192288955

Start: 573957

End: 574883

Strand: Reverse

Name: ispA [C]

Synonym: Rpal_0525

Alternate gene names: 192288955

Gene position: 574883-573957 (Counterclockwise)

Preceding gene: 192288959

Following gene: 192288954

Centisome position: 10.01

GC content: 69.8

Gene sequence:

>927_bases
ATGGCCACCGGCACTTCCACGATCGACTTTGCCAAGCGGCTCGACACTACCGCCGAGGAAACCGAAGCACTGCTCGGCCG
GCTGTTGTCCGACGAATTGATGTCCGACGAGATCGCCCGGCCGCGCCGGCTGATGGACGCGATCCGCTACTCCACGCTCG
GCGGCGGCAAGCGGCTGCGGCCGTTCCTGGCGGTCGAGAGCGCCGCAGTGTTCGGCATCGATCGTGCCGCCGCGCTCTTG
GTCGGCGCCGCGCTGGAATGCATCCACTGCTACTCGCTGATCCACGACGACCTGCCTTCGATGGACAACAGTGATCTGCG
CCGCGGCCGTCCGACACTGCACAAGGCCTATGACGACGCCACTGCGATCCTCGCCGGCGACGCGCTGCTGACCTTCGCGT
TCGACATCGTCACCCGCGACGAGGTGCACAACGATCCGACGGTGCGGCTGCTGCTGACACGAGTGCTGGCGCGCTGCGCC
GGGCTCGGCGGCATGGTCGGCGGCCAGATGCTTGATCTTGCCGGAGAAGGCCGGTTCGGCGACCGCGAGCCGGTCGACGT
CGCGCGGCTGCAGCAGATGAAGACCGGCGCCCTGCTCCGCTATGGCTGCGTCGCCGGCGCGATCCTCGGCCAGGCGAGCC
CCGACCAGTATCGCGCGCTCGACGATTACGGCCGCGCGCTCGGTGAAGCGTTCCAGATCGCCGACGACCTACTCGATGTC
GAAGGCGACGCTGCCGCGCTCGGCAAGCCGGCCGGAGCCGATGCCGCGCTCGGCAAGACCACCTTCGTCACCCAACTCGG
CATCGACGGCGCCAAGCAGCGGCTGCGCGACCTGATCGCGCGTGCCGACCAGGCGCTGGCGCCGTTCGGCGAGCGCGGCG
AGGTGCTGCGCGCCGCCGCCCGCTTCGTCGCCGAGCGGAAGAGCTGA

Upstream 100 bases:

>100_bases
ATCGCTCCCGGTGATGGCCTGGCCCCTTGCCGTCCGCCGAATTGACGAAGCCGGTGCCATGATGAATGGTCCGGCCGAAT
TTTATCTGGAGCACTTCCGA

Downstream 100 bases:

>100_bases
TCGGCGCATGACAGAGCACCCGGACGACGATCCGCTGCTGCTTCGCTTTCGGAAGCTGCCGCGGATCGTTCGCCTGGTGT
ATTCGCGGCCGCGGCTGTTC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MATGTSTIDFAKRLDTTAEETEALLGRLLSDELMSDEIARPRRLMDAIRYSTLGGGKRLRPFLAVESAAVFGIDRAAALL
VGAALECIHCYSLIHDDLPSMDNSDLRRGRPTLHKAYDDATAILAGDALLTFAFDIVTRDEVHNDPTVRLLLTRVLARCA
GLGGMVGGQMLDLAGEGRFGDREPVDVARLQQMKTGALLRYGCVAGAILGQASPDQYRALDDYGRALGEAFQIADDLLDV
EGDAAALGKPAGADAALGKTTFVTQLGIDGAKQRLRDLIARADQALAPFGERGEVLRAAARFVAERKS

Sequences:

>Translated_308_residues
MATGTSTIDFAKRLDTTAEETEALLGRLLSDELMSDEIARPRRLMDAIRYSTLGGGKRLRPFLAVESAAVFGIDRAAALL
VGAALECIHCYSLIHDDLPSMDNSDLRRGRPTLHKAYDDATAILAGDALLTFAFDIVTRDEVHNDPTVRLLLTRVLARCA
GLGGMVGGQMLDLAGEGRFGDREPVDVARLQQMKTGALLRYGCVAGAILGQASPDQYRALDDYGRALGEAFQIADDLLDV
EGDAAALGKPAGADAALGKTTFVTQLGIDGAKQRLRDLIARADQALAPFGERGEVLRAAARFVAERKS
>Mature_307_residues
ATGTSTIDFAKRLDTTAEETEALLGRLLSDELMSDEIARPRRLMDAIRYSTLGGGKRLRPFLAVESAAVFGIDRAAALLV
GAALECIHCYSLIHDDLPSMDNSDLRRGRPTLHKAYDDATAILAGDALLTFAFDIVTRDEVHNDPTVRLLLTRVLARCAG
LGGMVGGQMLDLAGEGRFGDREPVDVARLQQMKTGALLRYGCVAGAILGQASPDQYRALDDYGRALGEAFQIADDLLDVE
GDAAALGKPAGADAALGKTTFVTQLGIDGAKQRLRDLIARADQALAPFGERGEVLRAAARFVAERKS

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=225, Percent_Identity=30.6666666666667, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786623, Length=309, Percent_Identity=40.1294498381877, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1789578, Length=213, Percent_Identity=34.7417840375587, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17505681, Length=282, Percent_Identity=26.241134751773, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24651612, Length=257, Percent_Identity=28.4046692607004, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32916; Mature: 32785

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTSTIDFAKRLDTTAEETEALLGRLLSDELMSDEIARPRRLMDAIRYSTLGGGKRLR
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
PFLAVESAAVFGIDRAAALLVGAALECIHCYSLIHDDLPSMDNSDLRRGRPTLHKAYDDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHH
TAILAGDALLTFAFDIVTRDEVHNDPTVRLLLTRVLARCAGLGGMVGGQMLDLAGEGRFG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHCCCCCCC
DREPVDVARLQQMKTGALLRYGCVAGAILGQASPDQYRALDDYGRALGEAFQIADDLLDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
EGDAAALGKPAGADAALGKTTFVTQLGIDGAKQRLRDLIARADQALAPFGERGEVLRAAA
CCCHHHCCCCCCCCHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RFVAERKS
HHHHHCCC
>Mature Secondary Structure 
ATGTSTIDFAKRLDTTAEETEALLGRLLSDELMSDEIARPRRLMDAIRYSTLGGGKRLR
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
PFLAVESAAVFGIDRAAALLVGAALECIHCYSLIHDDLPSMDNSDLRRGRPTLHKAYDDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHH
TAILAGDALLTFAFDIVTRDEVHNDPTVRLLLTRVLARCAGLGGMVGGQMLDLAGEGRFG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCHHHHHCCCCCCC
DREPVDVARLQQMKTGALLRYGCVAGAILGQASPDQYRALDDYGRALGEAFQIADDLLDV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
EGDAAALGKPAGADAALGKTTFVTQLGIDGAKQRLRDLIARADQALAPFGERGEVLRAAA
CCCHHHCCCCCCCCHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RFVAERKS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]