Click here to switch to the map view.

The map label for this gene is trpS [H]

Identifier: 192288890

GI number: 192288890

Start: 492898

End: 493950

Strand: Reverse

Name: trpS [H]

Synonym: Rpal_0459

Alternate gene names: 192288890

Gene position: 493950-492898 (Counterclockwise)

Preceding gene: 192288891

Following gene: 192288889

Centisome position: 8.6

GC content: 62.58

Gene sequence:

>1053_bases
ATGGCCATCACGCAGCGGGTATTCTCCGGCATCCAGCCGACCGGCAATCTGCATCTCGGCAATTACCTCGGCGCGATCGT
GAACTTCGTGAAGATGCAGGAGACGCACAATTGCGTTTACTGCGTCGTCGATCTTCACGCCATCACCGTCCCGGTGACGG
TCTGGGGCGGACCGGACGAGCTGCGCAAGAACATCCGTGAAGTCACGGCGGCGTTCATCGCGTCCGGCATCGATCCGAAG
AAGCAGATCATCTTCAACCAGAGCCAGGTGGCGGAACATTCGGAACTGGCGTGGGTGCTGAACTGCATCGCGCGGCTCGG
CTGGCTGAATCGCATGACCCAGTTCAAGGAGAAGGCCGGTAAGGATCGCGAGAACGTGTCGGTCGGCCTGTACGATTATC
CGGTGCTGATGGCGGCCGACATTCTGGTGTACCGCGCCACCCACGTTCCGGTCGGCGAGGATCAGAAGCAACATGTCGAA
CTCACCCGCGACATCGCCCAGAAGTTCAACAATGACTTCTCGGCATCGATTGCGGCGCAGGGCTTCGGCGACGCTTACTT
CCCGCTGCCCGAGCCGGTGATCACCGGCCCGGCGACCCGGGTGATGAGCCTGCGCGACGGCACCAAGAAGATGTCGAAGT
CGGATCCGTCCGATTACTCGCGCATCAATCTGACCGACGATGCCGACGTGATCGCGCAGAAGATCAAGAAGGCCAAGACC
GATCCGGAGCCGCTGCCGAGCGAAGAAAAGGGCCTCGAGACGAGGCCCGAGGCGGATAACCTGGTCGGCATCTACGCGGC
GCTCTCCGGCAAGCCGAAGACCGACGTGCTGACCGAGTTCGGCGGCGCACAGTTCTCCGCGTTCAAGTCGAACCTGGTCG
ATCTCGCCGTCGCCAAGCTGTCGCCGATCGCCGACGAAATGAAGCGGCTCTCGGCCGACCATGCGTATGTCGACTCGGTG
CTCGCCGACGGCAGCGACCGCGCCCGTGCGATCGCGGCCGAAACCATGCGGGCCGTGAAGGACATCATGGGCATGGTGCG
CAAGACGAGCTGA

Upstream 100 bases:

>100_bases
GCGGCGCTCCATGCGCTGCGGCGGCCACGCGGCTTGCGCGACTAGCGGAGCTGTGCCACTTCACGGCGCCTTTTCCAGAC
CAAGCGCGGGAATCACGATC

Downstream 100 bases:

>100_bases
GGCGCCCTCTTCTGCAGCGTCCGTCGTAGCCGGGCTCGCCCCGGCCATCCATCATCTTGGCAGGACTGTTGCTAAGGTGG
ATGGACGCGCGGGCCTTCGC

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAITQRVFSGIQPTGNLHLGNYLGAIVNFVKMQETHNCVYCVVDLHAITVPVTVWGGPDELRKNIREVTAAFIASGIDPK
KQIIFNQSQVAEHSELAWVLNCIARLGWLNRMTQFKEKAGKDRENVSVGLYDYPVLMAADILVYRATHVPVGEDQKQHVE
LTRDIAQKFNNDFSASIAAQGFGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADVIAQKIKKAKT
DPEPLPSEEKGLETRPEADNLVGIYAALSGKPKTDVLTEFGGAQFSAFKSNLVDLAVAKLSPIADEMKRLSADHAYVDSV
LADGSDRARAIAAETMRAVKDIMGMVRKTS

Sequences:

>Translated_350_residues
MAITQRVFSGIQPTGNLHLGNYLGAIVNFVKMQETHNCVYCVVDLHAITVPVTVWGGPDELRKNIREVTAAFIASGIDPK
KQIIFNQSQVAEHSELAWVLNCIARLGWLNRMTQFKEKAGKDRENVSVGLYDYPVLMAADILVYRATHVPVGEDQKQHVE
LTRDIAQKFNNDFSASIAAQGFGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADVIAQKIKKAKT
DPEPLPSEEKGLETRPEADNLVGIYAALSGKPKTDVLTEFGGAQFSAFKSNLVDLAVAKLSPIADEMKRLSADHAYVDSV
LADGSDRARAIAAETMRAVKDIMGMVRKTS
>Mature_349_residues
AITQRVFSGIQPTGNLHLGNYLGAIVNFVKMQETHNCVYCVVDLHAITVPVTVWGGPDELRKNIREVTAAFIASGIDPKK
QIIFNQSQVAEHSELAWVLNCIARLGWLNRMTQFKEKAGKDRENVSVGLYDYPVLMAADILVYRATHVPVGEDQKQHVEL
TRDIAQKFNNDFSASIAAQGFGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADVIAQKIKKAKTD
PEPLPSEEKGLETRPEADNLVGIYAALSGKPKTDVLTEFGGAQFSAFKSNLVDLAVAKLSPIADEMKRLSADHAYVDSVL
ADGSDRARAIAAETMRAVKDIMGMVRKTS

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=344, Percent_Identity=42.1511627906977, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI41352700, Length=192, Percent_Identity=50.5208333333333, Blast_Score=205, Evalue=5e-53,
Organism=Escherichia coli, GI1789786, Length=342, Percent_Identity=42.3976608187134, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71982800, Length=347, Percent_Identity=34.0057636887608, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI71982793, Length=347, Percent_Identity=34.0057636887608, Blast_Score=200, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6320474, Length=359, Percent_Identity=37.883008356546, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24666151, Length=343, Percent_Identity=39.9416909620991, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 38353; Mature: 38222

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITQRVFSGIQPTGNLHLGNYLGAIVNFVKMQETHNCVYCVVDLHAITVPVTVWGGPDE
CCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEECCHHH
LRKNIREVTAAFIASGIDPKKQIIFNQSQVAEHSELAWVLNCIARLGWLNRMTQFKEKAG
HHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDRENVSVGLYDYPVLMAADILVYRATHVPVGEDQKQHVELTRDIAQKFNNDFSASIAAQ
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHC
GFGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADVIAQKIKKAKT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCC
DPEPLPSEEKGLETRPEADNLVGIYAALSGKPKTDVLTEFGGAQFSAFKSNLVDLAVAKL
CCCCCCCHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SPIADEMKRLSADHAYVDSVLADGSDRARAIAAETMRAVKDIMGMVRKTS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AITQRVFSGIQPTGNLHLGNYLGAIVNFVKMQETHNCVYCVVDLHAITVPVTVWGGPDE
CCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEECCHHH
LRKNIREVTAAFIASGIDPKKQIIFNQSQVAEHSELAWVLNCIARLGWLNRMTQFKEKAG
HHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDRENVSVGLYDYPVLMAADILVYRATHVPVGEDQKQHVELTRDIAQKFNNDFSASIAAQ
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHC
GFGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADVIAQKIKKAKT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCC
DPEPLPSEEKGLETRPEADNLVGIYAALSGKPKTDVLTEFGGAQFSAFKSNLVDLAVAKL
CCCCCCCHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
SPIADEMKRLSADHAYVDSVLADGSDRARAIAAETMRAVKDIMGMVRKTS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]