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The map label for this gene is pnp [H]

Identifier: 192288867

GI number: 192288867

Start: 468727

End: 470895

Strand: Reverse

Name: pnp [H]

Synonym: Rpal_0436

Alternate gene names: 192288867

Gene position: 470895-468727 (Counterclockwise)

Preceding gene: 192288868

Following gene: 192288866

Centisome position: 8.2

GC content: 64.87

Gene sequence:

>2169_bases
ATGTTCAATATCCATTCCGTGGAAATCGATTGGGGTGGCCGTCCCCTCAAGCTTGAAACCGGCAAGGTCGCCCGCCAGGC
CGACGGCGCCGTCGTCGCGACCTATGGCGAGACCGTCGTGCTCGCCACAGTGGTGGCCGCCAAGTCGCCCCGTGAAGGCG
TCGACTTCCTCCCCCTCACCGTCGACTACCAAGAAAAGGCCTACGCGGCCGGCCGCATTCCCGGCGGCTATTTCAAGCGT
GAAGGCCGTCCGACCGAGAAGGAGACTCTGGTCTCCCGTCTGATCGACCGCCCGATCCGTCCGCTGTTCGCCGACGGCTG
GCGCAACGAAACCCAGGTCATCGTCACCGTTCTGTCGCACGACATGGAGAACGATCCGGACGTGCTGGCGATGGTCGCCG
CCTCCGCGGCGCTGACGCTGTCCGGCGTGCCGTTCAAGGGTCCGATCGGCGCCGCCCGCGTCGGCTTCATCAACGACGAA
TACGTGCTCAATCCCGTGCTCGACGAGATGGCCGAAACCCAGCTTGAGCTGGTGGTTGCCGGTACCGCCGACGCGGTGCT
GATGGTCGAATCCGAAGCCAAGGAGCTGTCGGAAGAGATCATGCTCGGCGCGGTGATGTTCGGTCACCGCCACTTCCAGC
CGGTGATCGATGCGATCATCGACCTCGCCGAGAAGGCCGCCAAGGAGCCGCGCGAACTCACCGTCGTCGACGACAGCGAG
ATCGAAAAGGAAATGCTCGGCCTGGTCGAGCAGGAGCTGCGTGCCGCCTACGCCATCCCGGTCAAGCAGGACCGCTACGC
CGCGGTCGGCAAGGTCAAGGAGAAGGCGATTGCGCACTTCTTCCCGGAAGGCCAGGAGCCGAAATACGACAAGCTGCGCA
TCGCCGGCGTGTTCAAGGAGCTCGAGGCCAAGATCGTTCGCTGGAACATCCTCGACACCGGCAAGCGCATCGACGGCCGT
GACTCAAAGACCGTCCGCAACATCCTGGCTCAGGTCGGCGTGCTGCCGCGCACCCACGGTTCGGCGCTGTTCACCCGCGG
TGAGACCCAGGCGCTGGTCGTGACCACGCTCGGCACCGGCGAAGACGAGCAGTATGTCGACTCGCTGTCGGGAACGTACA
AAGAGACGTTCCTGCTGCACTACAACTTCCCGCCCTACTCGGTCGGTGAGACCGGTCGCCTCGGTGGTACCAAGCGCCGC
GAGATCGGCCACGGCAAGCTGGCGTGGCGCGCGATCCATCCGGTGCTGCCGCCGCATCACGAGTTCCCCTACACCATCCG
CGTCGTCTCCGAGATCACCGAGTCGAACGGCTCGTCCTCGATGGCGTCGGTGTGCGGCGCCTCGCTGGCGCTGATGGACG
CGGGCGTGCCGCTGAAGCGGCCGACCGCGGGTATCGCGATGGGCCTGATCCTGGAAGGTGAGCGCTTCGCCGTGCTGTCC
GACATCCTCGGCGACGAGGACCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACCGAGCAGGGCATCACCTCGCTGCA
GATGGACATCAAGATCGCCGGCATCACCGAAGAGATCATGAAGGTGGCGCTCGGCCAGGCCAAGGACGGTCGCATCCACA
TCCTGGGTGAGATGTCCAAGGCGCTCGACCGCGCCCGCGCCGAGCTCGGCGAACACGCGCCGCGCATCGAGACCTTCAAG
ATCCCGACCGACAAGATCCGCGAAGTGATCGGCACCGGCGGCAAGGTGATCCGCGAGATCGTCGAGAAGACCGGCGCCAA
GGTCAACATCGAGGACGACGGCACCGTCAAGGTCGCCTCCAGCGATGGTGAGTCGATCAAGGCTGCTATCAAGTGGATCA
AGTCGATCGCCTCCGATCCGGAGATCGGCGAGATCTACGAGGGCACCGTCGTCAAGGTGATGGAGTTCGGCGCCTTCGTG
AACTTCTTCGGCGCCAAGGACGGCCTGGTACACATCAGCCAGCTCGCCGCCGGCCGCGTGCAGAAGACCTCCGACGTCGT
CAAGGAAGGCGACAAGGTCAAGGTCAAGCTGCTCGGCTTCGACGACCGCGGCAAGACCCGGCTGTCGATGAAGGTGGTCG
ATCAGACCACCGGTGAAGACCTCGAAGCCAAGCAGAAGGCGGAAGCCAAGGCCGAAGGCGAAGCGCCCGCGCAGGCTGCC
GGCGAGTAA

Upstream 100 bases:

>100_bases
GCTGTTTGTGCAAACGCGCAGTCAGCGTCTCGCCATCTTGCGCATGGTCTTTTGGTATTTGGCGTCCGGTCTTCCCGAAA
ACCGATGAAAGAAAGACCCG

Downstream 100 bases:

>100_bases
GCCTTCCGGCGCGACACCGTCGCAGCAAGTCATCATCGATCAAGGGCGGCCTCACCGGGCCGCCCTTTTTCTTTGCGGCG
CCTTCCGTACGACAGCGGAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MFNIHSVEIDWGGRPLKLETGKVARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKAYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSHDMENDPDVLAMVAASAALTLSGVPFKGPIGAARVGFINDE
YVLNPVLDEMAETQLELVVAGTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIDLAEKAAKEPRELTVVDDSE
IEKEMLGLVEQELRAAYAIPVKQDRYAAVGKVKEKAIAHFFPEGQEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGR
DSKTVRNILAQVGVLPRTHGSALFTRGETQALVVTTLGTGEDEQYVDSLSGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIHPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEIGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLAAGRVQKTSDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKAEAKAEGEAPAQAA
GE

Sequences:

>Translated_722_residues
MFNIHSVEIDWGGRPLKLETGKVARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKAYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSHDMENDPDVLAMVAASAALTLSGVPFKGPIGAARVGFINDE
YVLNPVLDEMAETQLELVVAGTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIDLAEKAAKEPRELTVVDDSE
IEKEMLGLVEQELRAAYAIPVKQDRYAAVGKVKEKAIAHFFPEGQEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGR
DSKTVRNILAQVGVLPRTHGSALFTRGETQALVVTTLGTGEDEQYVDSLSGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIHPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEIGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLAAGRVQKTSDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKAEAKAEGEAPAQAA
GE
>Mature_722_residues
MFNIHSVEIDWGGRPLKLETGKVARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLTVDYQEKAYAAGRIPGGYFKR
EGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSHDMENDPDVLAMVAASAALTLSGVPFKGPIGAARVGFINDE
YVLNPVLDEMAETQLELVVAGTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIDLAEKAAKEPRELTVVDDSE
IEKEMLGLVEQELRAAYAIPVKQDRYAAVGKVKEKAIAHFFPEGQEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGR
DSKTVRNILAQVGVLPRTHGSALFTRGETQALVVTTLGTGEDEQYVDSLSGTYKETFLLHYNFPPYSVGETGRLGGTKRR
EIGHGKLAWRAIHPVLPPHHEFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSKALDRARAELGEHAPRIETFK
IPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVASSDGESIKAAIKWIKSIASDPEIGEIYEGTVVKVMEFGAFV
NFFGAKDGLVHISQLAAGRVQKTSDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKAEAKAEGEAPAQAA
GE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=38.4401114206128, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=51.0869565217391, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI21361576, Length=87, Percent_Identity=47.1264367816092, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=56.7723342939481, Blast_Score=771, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=711, Percent_Identity=34.5991561181435, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535281, Length=88, Percent_Identity=48.8636363636364, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320850, Length=109, Percent_Identity=34.8623853211009, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=38.857938718663, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=38.857938718663, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=38.857938718663, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=38.4732824427481, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=46.1538461538462, Blast_Score=76, Evalue=8e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78355; Mature: 78355

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNIHSVEIDWGGRPLKLETGKVARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLT
CCCEEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCCEEEHHHHHCCCCCCCCCEEEEE
VDYQEKAYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSH
ECCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHEEEEEECC
DMENDPDVLAMVAASAALTLSGVPFKGPIGAARVGFINDEYVLNPVLDEMAETQLELVVA
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEEEE
GTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIDLAEKAAKEPRELTVVDDSE
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
IEKEMLGLVEQELRAAYAIPVKQDRYAAVGKVKEKAIAHFFPEGQEPKYDKLRIAGVFKE
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LEAKIVRWNILDTGKRIDGRDSKTVRNILAQVGVLPRTHGSALFTRGETQALVVTTLGTG
HCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLSGTYKETFLLHYNFPPYSVGETGRLGGTKRREIGHGKLAWRAIHPVLPPHH
CCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHH
ALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGESIKAAIKWIKSIASDPEIGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLAAGRV
CCCHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
QKTSDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKAEAKAEGEAPAQAA
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
GE
CC
>Mature Secondary Structure
MFNIHSVEIDWGGRPLKLETGKVARQADGAVVATYGETVVLATVVAAKSPREGVDFLPLT
CCCEEEEEEECCCEEEEEECCCHHHHCCCCEEEECCCCEEEHHHHHCCCCCCCCCEEEEE
VDYQEKAYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPLFADGWRNETQVIVTVLSH
ECCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHEEEEEECC
DMENDPDVLAMVAASAALTLSGVPFKGPIGAARVGFINDEYVLNPVLDEMAETQLELVVA
CCCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEEEE
GTADAVLMVESEAKELSEEIMLGAVMFGHRHFQPVIDAIIDLAEKAAKEPRELTVVDDSE
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHH
IEKEMLGLVEQELRAAYAIPVKQDRYAAVGKVKEKAIAHFFPEGQEPKYDKLRIAGVFKE
HHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LEAKIVRWNILDTGKRIDGRDSKTVRNILAQVGVLPRTHGSALFTRGETQALVVTTLGTG
HCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLSGTYKETFLLHYNFPPYSVGETGRLGGTKRREIGHGKLAWRAIHPVLPPHH
CCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
EFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPLKRPTAGIAMGLILEGERFAVLS
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTEQGITSLQMDIKIAGITEEIMKVALGQAKDGRIHILGEMSK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHH
ALDRARAELGEHAPRIETFKIPTDKIREVIGTGGKVIREIVEKTGAKVNIEDDGTVKVAS
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SDGESIKAAIKWIKSIASDPEIGEIYEGTVVKVMEFGAFVNFFGAKDGLVHISQLAAGRV
CCCHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCH
QKTSDVVKEGDKVKVKLLGFDDRGKTRLSMKVVDQTTGEDLEAKQKAEAKAEGEAPAQAA
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA