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The map label for this gene is paaG [C]

Identifier: 192288851

GI number: 192288851

Start: 451429

End: 452241

Strand: Reverse

Name: paaG [C]

Synonym: Rpal_0420

Alternate gene names: 192288851

Gene position: 452241-451429 (Counterclockwise)

Preceding gene: 192288852

Following gene: 192288850

Centisome position: 7.87

GC content: 66.54

Gene sequence:

>813_bases
TTGAGCTATCAGGACATCCTCTATGAGGTCGCCGACAAAATCGCGACTATCACGCTGAACCGGCCGGACCGCATGAATGC
GTGGACGCCGGTGATGGAGCGCGAGGTTCGGACGGCGATGCAGCAGGCGGCCGCTGACAGCGAGGTGCGCGTCATCGTGC
TGACCGGCGCCGGCCGCGGCTTCTGTGCCGGCGCCGACATGCAGGTGCTGCAGACCATCGATCCGTCCGACGTGCGCCGT
GCCACCGACCTGCCGCCGTTCGACATGAATCGTCGCCCCGATTGGCAGACGCGCTACGCGTTCTATCCGTCGATTCCCAA
GCCGATCATCGGCATGCTCAACGGCGCCACGGCGGGCATCGGTCTGGTGCACGCGCTGTATTGCGACGTTCGCTTCGCCG
CCGACTCTGCGGTGTTCACCACCGCCTTTGCGCGCCGCGGACTGATCGCCGAGCACGGCATCTCCTGGATGCTGCCGCGG
ATCGTCGGCCACGCCAACGCGCTCGACCTGCTGCTGTCGGCGCGCCGGGTCTCGGCCGAGGAGGCGCTGCGGATGGGGCT
CGTCAACCGGCTCTATCCGATCGATCAGCTCCGCGAGCAGACCTATGCCTACGCCCGCGACCTCGCCGACAACGTCGCCC
CGGCCTCGATGCAGGTGATCAAGCGCCAGCTCTACGAGGTGCCGTTCCAGACGCTGGCGGAAGCCACGATCGACGCCAAT
CGCGAGATGGCGATCTCGCTCGCCAGCGACGATTTCAAAGAAGGCGTCGCCAGCTTCGTCGAAAAGCGCCCGCCGCGATT
CAGCAGCGTGTGA

Upstream 100 bases:

>100_bases
AGGCACGCGCCGCACCTGAGATCTGTCGCCCACGGCAGGCGGAGACAAGCACGGACGAACTCCGTGCACCCAACAACAAT
CAACAAAGGGAGAAACGCCG

Downstream 100 bases:

>100_bases
TCGTCAGGCTCAGGCCGGGCGTCTCGAACAGGGCGCTCGGCCGCCAAGGCGCGCGGCGACTGGCGGCCGGCGATCTCGCT
CGGACAAGCAGATCCACGGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSYQDILYEVADKIATITLNRPDRMNAWTPVMEREVRTAMQQAAADSEVRVIVLTGAGRGFCAGADMQVLQTIDPSDVRR
ATDLPPFDMNRRPDWQTRYAFYPSIPKPIIGMLNGATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPR
IVGHANALDLLLSARRVSAEEALRMGLVNRLYPIDQLREQTYAYARDLADNVAPASMQVIKRQLYEVPFQTLAEATIDAN
REMAISLASDDFKEGVASFVEKRPPRFSSV

Sequences:

>Translated_270_residues
MSYQDILYEVADKIATITLNRPDRMNAWTPVMEREVRTAMQQAAADSEVRVIVLTGAGRGFCAGADMQVLQTIDPSDVRR
ATDLPPFDMNRRPDWQTRYAFYPSIPKPIIGMLNGATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPR
IVGHANALDLLLSARRVSAEEALRMGLVNRLYPIDQLREQTYAYARDLADNVAPASMQVIKRQLYEVPFQTLAEATIDAN
REMAISLASDDFKEGVASFVEKRPPRFSSV
>Mature_269_residues
SYQDILYEVADKIATITLNRPDRMNAWTPVMEREVRTAMQQAAADSEVRVIVLTGAGRGFCAGADMQVLQTIDPSDVRRA
TDLPPFDMNRRPDWQTRYAFYPSIPKPIIGMLNGATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPRI
VGHANALDLLLSARRVSAEEALRMGLVNRLYPIDQLREQTYAYARDLADNVAPASMQVIKRQLYEVPFQTLAEATIDANR
EMAISLASDDFKEGVASFVEKRPPRFSSV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=29.6875, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI70995211, Length=245, Percent_Identity=27.7551020408163, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI260274832, Length=261, Percent_Identity=23.7547892720307, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI45643119, Length=261, Percent_Identity=23.7547892720307, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260275230, Length=261, Percent_Identity=23.7547892720307, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=27.5193798449612, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI221136756, Length=209, Percent_Identity=28.7081339712919, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI221136753, Length=209, Percent_Identity=28.7081339712919, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI221307494, Length=209, Percent_Identity=28.7081339712919, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=29.6703296703297, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=27.1794871794872, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=27.1794871794872, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=246, Percent_Identity=26.0162601626016, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI116235487, Length=199, Percent_Identity=28.643216080402, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757968, Length=210, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=210, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=223, Percent_Identity=27.8026905829596, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=33.5849056603774, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788597, Length=275, Percent_Identity=32.7272727272727, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787659, Length=271, Percent_Identity=30.9963099630996, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=29.296875, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082183, Length=283, Percent_Identity=27.9151943462898, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=29.1866028708134, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=31.5384615384615, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=28.125, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=268, Percent_Identity=29.4776119402985, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=225, Percent_Identity=30.2222222222222, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=30.316742081448, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=25.2, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=179, Percent_Identity=31.2849162011173, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=26.2626262626263, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI20129971, Length=274, Percent_Identity=31.3868613138686, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24653477, Length=274, Percent_Identity=31.3868613138686, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24654903, Length=261, Percent_Identity=23.3716475095785, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=29.2307692307692, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357171, Length=193, Percent_Identity=32.6424870466321, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=28.2352941176471, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922422, Length=269, Percent_Identity=25.6505576208178, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30057; Mature: 29926

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQDILYEVADKIATITLNRPDRMNAWTPVMEREVRTAMQQAAADSEVRVIVLTGAGRG
CCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FCAGADMQVLQTIDPSDVRRATDLPPFDMNRRPDWQTRYAFYPSIPKPIIGMLNGATAGI
EECCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCHHHH
GLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPRIVGHANALDLLLSARRVSAE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
EALRMGLVNRLYPIDQLREQTYAYARDLADNVAPASMQVIKRQLYEVPFQTLAEATIDAN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
REMAISLASDDFKEGVASFVEKRPPRFSSV
CEEEEEECCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYQDILYEVADKIATITLNRPDRMNAWTPVMEREVRTAMQQAAADSEVRVIVLTGAGRG
CHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FCAGADMQVLQTIDPSDVRRATDLPPFDMNRRPDWQTRYAFYPSIPKPIIGMLNGATAGI
EECCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCHHHH
GLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPRIVGHANALDLLLSARRVSAE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
EALRMGLVNRLYPIDQLREQTYAYARDLADNVAPASMQVIKRQLYEVPFQTLAEATIDAN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
REMAISLASDDFKEGVASFVEKRPPRFSSV
CEEEEEECCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA