Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 192288775

GI number: 192288775

Start: 362809

End: 363741

Strand: Reverse

Name: ada [H]

Synonym: Rpal_0344

Alternate gene names: 192288775

Gene position: 363741-362809 (Counterclockwise)

Preceding gene: 192288776

Following gene: 192288774

Centisome position: 6.33

GC content: 68.7

Gene sequence:

>933_bases
GTGGCGGCGCGCGCCGCGGCGGATAACATCAGCGGTATGATGAACCTTGCCATTGCCGACCACCAGCTCATCAAACCCGG
CGCCCGCGATTCCGCGTTGGCCGACTACGACTGCGTGCGGCGCGCCATCGCCTTCATCTCGCAGAAATGGAAGGCGCAGC
CGACCATCGAGGCGATCGCCGACGCCGCCGGCCTGACGCCCGACGAGCTGCACCATCTGTTCCGGCGCTGGGCGGGGCTG
ACGCCGAAGGCGTTCATGCAGGCGCTGACGCTCGACCACGCCAAATCGCTGCTGCGGGATTCCGCCAGCGTGCTCGATGC
CGCGCTGGCCTCCGGGCTGTCCGGCCCCGGCCGACTGCACGATCTGTTCGTCACCCACGAGGCGATGTCGCCGGGCGAAT
GGAAGAGCGGCGGCGCCGGGCTCAGCCTGCGCTACGGCTTTCATCCGTCGCCGTTCGGCACCGCGGTGATCATCGCCTCC
GATCGCGGCCTTGCCGGTCTCGCCTTCGCCGACCCGGACGAGGAGCAGGCGGCGCTGGTCGATCTGCAACAGCGCTGGCC
GCGCGCGGTGTGCACGCAGGATCAGGACGCGACTGCTCCGTTGGCGCGGCGGATCTTCGATCCGGCACAATGGCGTGCCG
AGCAGCCGCTGCGGGTGGTGCTGATCGGCACCGATTTCGAAGTGCGGGTGTGGGAGACGCTGCTGAAGATCCCGCTCGGC
AAGGCGGTTTGCTACTCGGACATCGCCGCTAAGATCAGCCTACCGAAAGCCTCGCGCGCGGTCGGCGCCGCGGTCGGCAA
GAACCCGATCTCGTTCGTGGTGCCGTGCCATCGCGCGCTTGGCAAAGGCGGCGCACTCACCGGCTATCACTGGGGCCTGA
CCCGCAAGCAGGCGATGATCGGCTGGGAAGCCGGGCAACTCAGGGCAGAGTGA

Upstream 100 bases:

>100_bases
TCGGCGCGGAAGAAAAAGCGAACTTCGCCAAGGCCTTGATGGCGGCGCTCGACGCCGCGCGGCGCGGTCCGCTGTACCCG
GCCTGACACCAGGAAATCGG

Downstream 100 bases:

>100_bases
GCCGCAGGCCTAACCCGATTGACGGGGGCGGGTCGCGACCATAGGTGTGAAGAGCGCTCAGGCGCCCCCCACATCCAGGA
AAGTTCTCGCCATGCAGTCC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MAARAAADNISGMMNLAIADHQLIKPGARDSALADYDCVRRAIAFISQKWKAQPTIEAIADAAGLTPDELHHLFRRWAGL
TPKAFMQALTLDHAKSLLRDSASVLDAALASGLSGPGRLHDLFVTHEAMSPGEWKSGGAGLSLRYGFHPSPFGTAVIIAS
DRGLAGLAFADPDEEQAALVDLQQRWPRAVCTQDQDATAPLARRIFDPAQWRAEQPLRVVLIGTDFEVRVWETLLKIPLG
KAVCYSDIAAKISLPKASRAVGAAVGKNPISFVVPCHRALGKGGALTGYHWGLTRKQAMIGWEAGQLRAE

Sequences:

>Translated_310_residues
MAARAAADNISGMMNLAIADHQLIKPGARDSALADYDCVRRAIAFISQKWKAQPTIEAIADAAGLTPDELHHLFRRWAGL
TPKAFMQALTLDHAKSLLRDSASVLDAALASGLSGPGRLHDLFVTHEAMSPGEWKSGGAGLSLRYGFHPSPFGTAVIIAS
DRGLAGLAFADPDEEQAALVDLQQRWPRAVCTQDQDATAPLARRIFDPAQWRAEQPLRVVLIGTDFEVRVWETLLKIPLG
KAVCYSDIAAKISLPKASRAVGAAVGKNPISFVVPCHRALGKGGALTGYHWGLTRKQAMIGWEAGQLRAE
>Mature_309_residues
AARAAADNISGMMNLAIADHQLIKPGARDSALADYDCVRRAIAFISQKWKAQPTIEAIADAAGLTPDELHHLFRRWAGLT
PKAFMQALTLDHAKSLLRDSASVLDAALASGLSGPGRLHDLFVTHEAMSPGEWKSGGAGLSLRYGFHPSPFGTAVIIASD
RGLAGLAFADPDEEQAALVDLQQRWPRAVCTQDQDATAPLARRIFDPAQWRAEQPLRVVLIGTDFEVRVWETLLKIPLGK
AVCYSDIAAKISLPKASRAVGAAVGKNPISFVVPCHRALGKGGALTGYHWGLTRKQAMIGWEAGQLRAE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=258, Percent_Identity=32.5581395348837, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=50, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=42.3529411764706, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=42.3529411764706, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=137, Percent_Identity=34.3065693430657, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17137554, Length=174, Percent_Identity=30.4597701149425, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 33299; Mature: 33167

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAAADNISGMMNLAIADHQLIKPGARDSALADYDCVRRAIAFISQKWKAQPTIEAIA
CCCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DAAGLTPDELHHLFRRWAGLTPKAFMQALTLDHAKSLLRDSASVLDAALASGLSGPGRLH
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
DLFVTHEAMSPGEWKSGGAGLSLRYGFHPSPFGTAVIIASDRGLAGLAFADPDEEQAALV
EEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHH
DLQQRWPRAVCTQDQDATAPLARRIFDPAQWRAEQPLRVVLIGTDFEVRVWETLLKIPLG
HHHHHCCHHHCCCCCCCHHHHHHHHCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
KAVCYSDIAAKISLPKASRAVGAAVGKNPISFVVPCHRALGKGGALTGYHWGLTRKQAMI
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCEECCCCHHHHHE
GWEAGQLRAE
ECCCCCCCCC
>Mature Secondary Structure 
AARAAADNISGMMNLAIADHQLIKPGARDSALADYDCVRRAIAFISQKWKAQPTIEAIA
CCCHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DAAGLTPDELHHLFRRWAGLTPKAFMQALTLDHAKSLLRDSASVLDAALASGLSGPGRLH
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
DLFVTHEAMSPGEWKSGGAGLSLRYGFHPSPFGTAVIIASDRGLAGLAFADPDEEQAALV
EEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHH
DLQQRWPRAVCTQDQDATAPLARRIFDPAQWRAEQPLRVVLIGTDFEVRVWETLLKIPLG
HHHHHCCHHHCCCCCCCHHHHHHHHCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
KAVCYSDIAAKISLPKASRAVGAAVGKNPISFVVPCHRALGKGGALTGYHWGLTRKQAMI
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCEECCCCHHHHHE
GWEAGQLRAE
ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]