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The map label for this gene is gacS [H]

Identifier: 192288750

GI number: 192288750

Start: 338482

End: 340392

Strand: Reverse

Name: gacS [H]

Synonym: Rpal_0319

Alternate gene names: 192288750

Gene position: 340392-338482 (Counterclockwise)

Preceding gene: 192288751

Following gene: 192288749

Centisome position: 5.93

GC content: 66.35

Gene sequence:

>1911_bases
ATGGCGGGCAAGCTGCGGATCAGGTCGAGGCTGCGTCTGTTGGCGCGCCGGCATCCGCGCATCACGATGGCGGGCCGCGC
CGTGATGGCGTTTTCCGCCGGGTTCGGCGGTGCTTATGGACTGCTGCTCGGCAGCAACCCGGAGACCTCCGGCTACGATC
CGCACGCCTTCGCCGTTGGCGCCAGTTTCCTGTTCGCGCTGGCCTGCTTGGCGCTTGCGGTGCAAACGATGCGGCTGCGG
CGAATGAGGGGCCGCCTGCGCCTGCTGACTGCGTCTCAGGAACCACCTCCGGATCTGCAGCCAAATCAAAGCAAGACACG
CGCGCTGATCGCGGCGCGCGAGAGCGCCGACGCCGCGAACCACGCCAAGTCGCGGTTGCTGGCAATGGCGTCGCACGAAA
TCCGCACCCCACTGAACGGCATCATCGGCATGAGCCGCCTGTTGCTCGATACGCCGCTCAGCGCCGAACAGGCGACTTAC
GCCAAGGCGGTGAAAACATCCGGCGAGGCGCTGCTGGCGTTGACGGACGAGTTGCTCGACTACGCGCGGATCGAGGCTGG
CAAGATCGAGCTCGATTGCCGGCCCTTCGTATTGAGCAGTCTGATCGAAGACATCATCGAATTGCTGGCGCCGCGGGCGC
AGGCGCGCGGACTCGAGATTGCGGCCGATATCGACGAGCGGCTACCGGTAAGCCTGATTGGCGATGCCGCACGGTTGCGT
CAGGTGCTGCTCAATCTCTGCGGCAACGCCATCAAATTCACTGAAACTGGCGGCGTCGCTCTGATCGTCGAGCGAGGCGA
CCAGAGCGACGGACTCCGCATCATCGTCCGTGACACCGGCATCGGCATTGCGCCCGACGCGCAGGCACGAATCTTCCACG
AGTTCGAACAGGCCGACGCCGGGATCGCCCGCAATTTCGGCGGCACCGGTCTCGGCCTCAGCATCAGCGACCGCATCGTC
TCGGTGATGGGTGGCCGGATCGCGCTCGAGAGTAAACCTGGGGAAGGCTCGACCTTCACCATCACGCTACCGCTGAGCGC
CGCCGACCACGATCGCGCAACCGCGGCATTTGCACCGCCGGATCTGCATGGCAGCTCGGTGATGATCGTCTCGCCGCACG
GGGTCGAAGCTTCGCTGGCCGCCCGGCGGTTGGAGCGCTGGGGCGCCGAGACCTGCCTGTGTGATGATCTCGCCGCGGCG
ATCGCCCGGCTGCCGGAGCGAAGCTGGCACGCGGTGCTGATCGATCATGCGTTCGGTACGGACACGATTGAGACCTTGGC
GCGCAAGGCTCTGCCGCGTGCGACCCATCGCCTGGTGATGCTGACGCCGGCGGCACGGCACGAGCTGCTGCCGGATCTGC
CCGCCGCGTTCACAGGCTATCTGGTGAAGCCGCTGCGGGCCGCGTCGCTTGCCGCACGGCTCGGCACGGCGGTCGAGGTC
GCCGCGCCAAGTATCGCAGACGGCGATCCGCCGCCCGTTCAGGATGCGTCCTCAGCCATCACGCATGAAGCGTTCGCAAT
CCTGGTCGCCGAAGATAACGACATCAATGCGCTGCTGATTCGCGCACTACTGCACCGGCTTGGCCATCACGCCGAGATCG
TGGGTGACGGCCGCCGTGCCGTCGAAGCGTGGGGCGCGGCTGATAACACCGGTACGCCGTTCGATCTGGTGCTGATGGAC
GTGCAGATGCCAATCCTCGATGGGCTGGCGGCGGCCCGACAGATCAGGACGCTCGAGGCTGAGCGGGCGAGGTCGCGGGT
GCCGATCCTGGCGCTGACCGCGAACACTCTGGCCGAGGATCGCGAGGCATGTTTGGAGTCCGGAATGGACGGATTCCTGG
TCAAGCCGATCGATCCGGATAGGCTCGCAGCGATCCTGGCGGGCGTGGCCGCCGTGCACCAAACCGCCTGA

Upstream 100 bases:

>100_bases
ATTCGCAGCGCTGAGGCCTTCCCGCCAAGGTTTCAACCTGTCTGCAGCACCCCAAACGCCATGTTCTGCGTTGCCGATAG
CGGCAACTGTGGGAAGGGCA

Downstream 100 bases:

>100_bases
CACGGCAGGCAAATACCGAGCGCCGCTGCTAACAACAAAGTCAGCAGCGATGTCATCGTTCGGCAATCATCGCGTCACAT
CGCTGTTGAACGGTCATGAT

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MAGKLRIRSRLRLLARRHPRITMAGRAVMAFSAGFGGAYGLLLGSNPETSGYDPHAFAVGASFLFALACLALAVQTMRLR
RMRGRLRLLTASQEPPPDLQPNQSKTRALIAARESADAANHAKSRLLAMASHEIRTPLNGIIGMSRLLLDTPLSAEQATY
AKAVKTSGEALLALTDELLDYARIEAGKIELDCRPFVLSSLIEDIIELLAPRAQARGLEIAADIDERLPVSLIGDAARLR
QVLLNLCGNAIKFTETGGVALIVERGDQSDGLRIIVRDTGIGIAPDAQARIFHEFEQADAGIARNFGGTGLGLSISDRIV
SVMGGRIALESKPGEGSTFTITLPLSAADHDRATAAFAPPDLHGSSVMIVSPHGVEASLAARRLERWGAETCLCDDLAAA
IARLPERSWHAVLIDHAFGTDTIETLARKALPRATHRLVMLTPAARHELLPDLPAAFTGYLVKPLRAASLAARLGTAVEV
AAPSIADGDPPPVQDASSAITHEAFAILVAEDNDINALLIRALLHRLGHHAEIVGDGRRAVEAWGAADNTGTPFDLVLMD
VQMPILDGLAAARQIRTLEAERARSRVPILALTANTLAEDREACLESGMDGFLVKPIDPDRLAAILAGVAAVHQTA

Sequences:

>Translated_636_residues
MAGKLRIRSRLRLLARRHPRITMAGRAVMAFSAGFGGAYGLLLGSNPETSGYDPHAFAVGASFLFALACLALAVQTMRLR
RMRGRLRLLTASQEPPPDLQPNQSKTRALIAARESADAANHAKSRLLAMASHEIRTPLNGIIGMSRLLLDTPLSAEQATY
AKAVKTSGEALLALTDELLDYARIEAGKIELDCRPFVLSSLIEDIIELLAPRAQARGLEIAADIDERLPVSLIGDAARLR
QVLLNLCGNAIKFTETGGVALIVERGDQSDGLRIIVRDTGIGIAPDAQARIFHEFEQADAGIARNFGGTGLGLSISDRIV
SVMGGRIALESKPGEGSTFTITLPLSAADHDRATAAFAPPDLHGSSVMIVSPHGVEASLAARRLERWGAETCLCDDLAAA
IARLPERSWHAVLIDHAFGTDTIETLARKALPRATHRLVMLTPAARHELLPDLPAAFTGYLVKPLRAASLAARLGTAVEV
AAPSIADGDPPPVQDASSAITHEAFAILVAEDNDINALLIRALLHRLGHHAEIVGDGRRAVEAWGAADNTGTPFDLVLMD
VQMPILDGLAAARQIRTLEAERARSRVPILALTANTLAEDREACLESGMDGFLVKPIDPDRLAAILAGVAAVHQTA
>Mature_635_residues
AGKLRIRSRLRLLARRHPRITMAGRAVMAFSAGFGGAYGLLLGSNPETSGYDPHAFAVGASFLFALACLALAVQTMRLRR
MRGRLRLLTASQEPPPDLQPNQSKTRALIAARESADAANHAKSRLLAMASHEIRTPLNGIIGMSRLLLDTPLSAEQATYA
KAVKTSGEALLALTDELLDYARIEAGKIELDCRPFVLSSLIEDIIELLAPRAQARGLEIAADIDERLPVSLIGDAARLRQ
VLLNLCGNAIKFTETGGVALIVERGDQSDGLRIIVRDTGIGIAPDAQARIFHEFEQADAGIARNFGGTGLGLSISDRIVS
VMGGRIALESKPGEGSTFTITLPLSAADHDRATAAFAPPDLHGSSVMIVSPHGVEASLAARRLERWGAETCLCDDLAAAI
ARLPERSWHAVLIDHAFGTDTIETLARKALPRATHRLVMLTPAARHELLPDLPAAFTGYLVKPLRAASLAARLGTAVEVA
APSIADGDPPPVQDASSAITHEAFAILVAEDNDINALLIRALLHRLGHHAEIVGDGRRAVEAWGAADNTGTPFDLVLMDV
QMPILDGLAAARQIRTLEAERARSRVPILALTANTLAEDREACLESGMDGFLVKPIDPDRLAAILAGVAAVHQTA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=524, Percent_Identity=34.9236641221374, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI145693157, Length=524, Percent_Identity=32.0610687022901, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI48994928, Length=367, Percent_Identity=34.3324250681199, Blast_Score=179, Evalue=7e-46,
Organism=Escherichia coli, GI87081816, Length=362, Percent_Identity=34.2541436464088, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788713, Length=252, Percent_Identity=34.9206349206349, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=32.5991189427313, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=31.7269076305221, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=30.5309734513274, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=28.6995515695067, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=220, Percent_Identity=29.0909090909091, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787894, Length=253, Percent_Identity=30.4347826086957, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=29.3859649122807, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=29.2035398230088, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=29.4372294372294, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789808, Length=260, Percent_Identity=28.4615384615385, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1790437, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=247, Percent_Identity=27.1255060728745, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=145, Percent_Identity=36.551724137931, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322000, Length=153, Percent_Identity=30.0653594771242, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67658; Mature: 67527

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKLRIRSRLRLLARRHPRITMAGRAVMAFSAGFGGAYGLLLGSNPETSGYDPHAFAVG
CCCCHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHH
ASFLFALACLALAVQTMRLRRMRGRLRLLTASQEPPPDLQPNQSKTRALIAARESADAAN
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHEEHHCCCHHH
HAKSRLLAMASHEIRTPLNGIIGMSRLLLDTPLSAEQATYAKAVKTSGEALLALTDELLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
YARIEAGKIELDCRPFVLSSLIEDIIELLAPRAQARGLEIAADIDERLPVSLIGDAARLR
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCCCHHHHCCHHHHH
QVLLNLCGNAIKFTETGGVALIVERGDQSDGLRIIVRDTGIGIAPDAQARIFHEFEQADA
HHHHHHCCCCEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCC
GIARNFGGTGLGLSISDRIVSVMGGRIALESKPGEGSTFTITLPLSAADHDRATAAFAPP
CHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCCCCEEECCCC
DLHGSSVMIVSPHGVEASLAARRLERWGAETCLCDDLAAAIARLPERSWHAVLIDHAFGT
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
DTIETLARKALPRATHRLVMLTPAARHELLPDLPAAFTGYLVKPLRAASLAARLGTAVEV
HHHHHHHHHHCCHHHCEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEE
AAPSIADGDPPPVQDASSAITHEAFAILVAEDNDINALLIRALLHRLGHHAEIVGDGRRA
CCCCCCCCCCCCCCHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHH
VEAWGAADNTGTPFDLVLMDVQMPILDGLAAARQIRTLEAERARSRVPILALTANTLAED
HHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
REACLESGMDGFLVKPIDPDRLAAILAGVAAVHQTA
HHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGKLRIRSRLRLLARRHPRITMAGRAVMAFSAGFGGAYGLLLGSNPETSGYDPHAFAVG
CCCHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHH
ASFLFALACLALAVQTMRLRRMRGRLRLLTASQEPPPDLQPNQSKTRALIAARESADAAN
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHEEHHCCCHHH
HAKSRLLAMASHEIRTPLNGIIGMSRLLLDTPLSAEQATYAKAVKTSGEALLALTDELLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
YARIEAGKIELDCRPFVLSSLIEDIIELLAPRAQARGLEIAADIDERLPVSLIGDAARLR
HHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCCCHHHHCCHHHHH
QVLLNLCGNAIKFTETGGVALIVERGDQSDGLRIIVRDTGIGIAPDAQARIFHEFEQADA
HHHHHHCCCCEEEECCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCC
GIARNFGGTGLGLSISDRIVSVMGGRIALESKPGEGSTFTITLPLSAADHDRATAAFAPP
CHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCEEEEEEEECCCCCCCCEEECCCC
DLHGSSVMIVSPHGVEASLAARRLERWGAETCLCDDLAAAIARLPERSWHAVLIDHAFGT
CCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
DTIETLARKALPRATHRLVMLTPAARHELLPDLPAAFTGYLVKPLRAASLAARLGTAVEV
HHHHHHHHHHCCHHHCEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEE
AAPSIADGDPPPVQDASSAITHEAFAILVAEDNDINALLIRALLHRLGHHAEIVGDGRRA
CCCCCCCCCCCCCCHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHH
VEAWGAADNTGTPFDLVLMDVQMPILDGLAAARQIRTLEAERARSRVPILALTANTLAED
HHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
REACLESGMDGFLVKPIDPDRLAAILAGVAAVHQTA
HHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]