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The map label for this gene is trmD

Identifier: 192288674

GI number: 192288674

Start: 259732

End: 260478

Strand: Reverse

Name: trmD

Synonym: Rpal_0243

Alternate gene names: 192288674

Gene position: 260478-259732 (Counterclockwise)

Preceding gene: 192288675

Following gene: 192288673

Centisome position: 4.53

GC content: 69.21

Gene sequence:

>747_bases
ATGACCTGGCGCGCCACTGTCCTCACCTTGTTTCCGGAGATGTTTCCCGGCCCGCTCGGCGTCAGTCTGGCGGGGCGGGC
GCTGGCGTCGGGGCTGTGGGGTCTGGAGGCCCGCGATATCCGGGACTCGGCCACTGACCGACACCGCAGCGTCGACGACA
CCCCGGCGGGCGGCGGCCCCGGCATGGTGCTGCGCGCCGACGTGCTGGCGGCCGCAATCGACGCCGTGGATGCTGCTGCG
GATCGCCCGCGTTTGGTGATGAGCCCGCGTGGTCGGCCATTGACCCAGGCACGCGTGGCCGAGCTTGCGGCCGGGCCCGG
GCCGCTGATCGTCTGTGGCCGGTTCGAAGGCATTGACCAGCGGGTGATCGACGCGCGCGGGCTCGAGGAAGTCTCGATCG
GCGATTATGTGCTGTCGGGCGGCGAGATCGCCGCGATGGCGCTGATCGACGCCTGCGTCCGGCTGCTGCCGGGCGTGATG
GGCAAGCTGGAATCCTCGACCGACGAGAGCTTTTCGGCCGGTCTGCTGGAATATCCGCAATACACCAGGCCGCAGACATT
CGAGGGCCGGGCGATCCCCGAGGTGCTGCTGTCGGGCGACCACGGCAAGGTTGCGGCCTGGCGGTTGGGCGAGGCCGAGG
CCCTGACCAAGGCCCGGCGGCCCGATTTGTGGGCCGCCAGACCGGCGCAAACCATTCGGGCAAAGGGCGAATCGCAAAAA
ACGCCAAAAAACAAGACGGACGGGTGA

Upstream 100 bases:

>100_bases
TTGTCGTCGCCGGGCTCGATCCGGCGATCCATCGTCTCGTGAAGATGATGGATGCCCGGATCAAGTCCGGGTATGACGCC
TCACCTGTTGCAGGCTCTGC

Downstream 100 bases:

>100_bases
CAAACCCCCAAGATTGCCGTATAGCACCGCCAAATCCGTGCAATGGCAGGACAATGGATCTTTGCGCAGCCCGCGCCGGG
ATGGTCGGGCGCGCCGCCGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTWRATVLTLFPEMFPGPLGVSLAGRALASGLWGLEARDIRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAVDAAA
DRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQRVIDARGLEEVSIGDYVLSGGEIAAMALIDACVRLLPGVM
GKLESSTDESFSAGLLEYPQYTRPQTFEGRAIPEVLLSGDHGKVAAWRLGEAEALTKARRPDLWAARPAQTIRAKGESQK
TPKNKTDG

Sequences:

>Translated_248_residues
MTWRATVLTLFPEMFPGPLGVSLAGRALASGLWGLEARDIRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAVDAAA
DRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQRVIDARGLEEVSIGDYVLSGGEIAAMALIDACVRLLPGVM
GKLESSTDESFSAGLLEYPQYTRPQTFEGRAIPEVLLSGDHGKVAAWRLGEAEALTKARRPDLWAARPAQTIRAKGESQK
TPKNKTDG
>Mature_247_residues
TWRATVLTLFPEMFPGPLGVSLAGRALASGLWGLEARDIRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAVDAAAD
RPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQRVIDARGLEEVSIGDYVLSGGEIAAMALIDACVRLLPGVMG
KLESSTDESFSAGLLEYPQYTRPQTFEGRAIPEVLLSGDHGKVAAWRLGEAEALTKARRPDLWAARPAQTIRAKGESQKT
PKNKTDG

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=219, Percent_Identity=49.7716894977169, Blast_Score=200, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RHOPT (B3Q854)

Other databases:

- EMBL:   CP001096
- RefSeq:   YP_001989279.1
- ProteinModelPortal:   B3Q854
- SMR:   B3Q854
- GeneID:   6407887
- GenomeReviews:   CP001096_GR
- KEGG:   rpt:Rpal_0243
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26307; Mature: 26176

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWRATVLTLFPEMFPGPLGVSLAGRALASGLWGLEARDIRDSATDRHRSVDDTPAGGGP
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GMVLRADVLAAAIDAVDAAADRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQ
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHH
RVIDARGLEEVSIGDYVLSGGEIAAMALIDACVRLLPGVMGKLESSTDESFSAGLLEYPQ
HHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHCCCC
YTRPQTFEGRAIPEVLLSGDHGKVAAWRLGEAEALTKARRPDLWAARPAQTIRAKGESQK
CCCCCCCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
TPKNKTDG
CCCCCCCC
>Mature Secondary Structure 
TWRATVLTLFPEMFPGPLGVSLAGRALASGLWGLEARDIRDSATDRHRSVDDTPAGGGP
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GMVLRADVLAAAIDAVDAAADRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQ
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHH
RVIDARGLEEVSIGDYVLSGGEIAAMALIDACVRLLPGVMGKLESSTDESFSAGLLEYPQ
HHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHCCCC
YTRPQTFEGRAIPEVLLSGDHGKVAAWRLGEAEALTKARRPDLWAARPAQTIRAKGESQK
CCCCCCCCCCCCHHHHCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
TPKNKTDG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA