Click here to switch to the map view.

The map label for this gene is sucB [H]

Identifier: 192288616

GI number: 192288616

Start: 198491

End: 199744

Strand: Reverse

Name: sucB [H]

Synonym: Rpal_0183

Alternate gene names: 192288616

Gene position: 199744-198491 (Counterclockwise)

Preceding gene: 192288617

Following gene: 192288615

Centisome position: 3.48

GC content: 66.75

Gene sequence:

>1254_bases
ATGACTGAAATTCGCGTTCCGACGCTCGGTGAATCGGTGACCGAGGCGACCATCGGCCGCTGGTTCAAGAAGCCTGGTGA
GGCGGTGGCTGTCGATGAGCCGCTCGTCGAGCTCGAAACCGACAAGGTGACCATTGAAGTGCCGGCCCCGTCGGCCGGTA
CGCTCGGCGAAATCGTCGCCAAGGACGGCGAGACCGTTGCGGTCGGCGCGTTGCTCGGCCAGATCACCGATGGCGCCGCC
CCGGCCAAGCCGGCTGCGGCTGCTCCGGCGAAGGCTGCGGCCCCGGCTGCTGCGCCTGCCCCGGCGGCACCGGCCCCCGC
GGCTGCTCCCGCCGCCAAGGCTCCGCCGTCCGATGCGCCGCTGGCGCCGTCGGTGCGCCGGCTGTCAACCGAGAGCGGCG
TCGACGCCTCGACCGTGCCGGGCTCCGGCAAGGACGGTCGCGTCACCAAGGGCGATATGCTGGCGGCGATCGAGAAGGCG
GCCTCGGCGCCGACCCCGGTCAATCAGCCTGCCGCCGCGGTGCAGGTCCGCGGGCCGTCTCCGGCCGACGACGCCGCACG
CGAAGAGCGGGTGAAGATGACCCGGCTGCGCCAGACCATCGCGCGCCGCCTGAAGGAAGTGCAGAACACCGCCGCGATGC
TGACGACCTTCAACGAGGTCGACATGACCAACGTCATGGCGCTGCGCGCGCAGTACAAGGACGTGTTCGAGAAGAAGCAC
GGCGCCAAGCTCGGCTTCATGGGCTTCTTCACCAAGGCCTGCGTTCAGGCCCTGAAGGATATCCCGGCGGTGAACGCCGA
GATGGACGGCACCGACCTGATCTACAAGAACTACTACCACATCGGCGTCGCCGTCGGCACCGACAAGGGTCTCGTGGTCC
CGGTGGTGCGCGACTGCGACGAGAAGTCGATCGCCGACATCGAAAAGAGCATCGTCGACTACGGCAAGCGTGCCCGTGAC
GGTCAGCTCAAGATCGAGGAGATGCAGGGCGGTACCTTCACGATCACTAACGGCGGGATCTACGGTTCGCTGATGTCGAC
CCCGATCCTGAACGCGCCGCAGTCCGGCATCCTCGGCATGCACAAGATCCAGGAACGCCCGGTTGTGATCGGCGGCAAGA
TCGAAGTCCGCCCGATGATGTATTTGGCGCTGAGCTATGATCACCGCGTGATCGACGGCAAGGAAGCCGTGACCTTCCTG
GTTCGCGTCAAGGAGAGCCTGGAAGACCCGGCGCGCCTGGTGCTGGATCTCTGA

Upstream 100 bases:

>100_bases
TCGGGCCGCCGCCTGACGGCGGCCCTTCGGTTTTTCCTGAGTTTGGATCACGGGGCGGGCGCCGGCGCGACAGCCGCGAC
GCTGCAAAAGGACGAAGACG

Downstream 100 bases:

>100_bases
CACTGGAGTAAGTGCCGGCGGCGTGCTCCTGCGCGCCGCCCTGACCTTGGGGGAAACGATGCCGCCGATGCCGACCGAGA
TCACGGTGCTGGGCTGGAGC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTEIRVPTLGESVTEATIGRWFKKPGEAVAVDEPLVELETDKVTIEVPAPSAGTLGEIVAKDGETVAVGALLGQITDGAA
PAKPAAAAPAKAAAPAAAPAPAAPAPAAAPAAKAPPSDAPLAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKA
ASAPTPVNQPAAAVQVRGPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRAQYKDVFEKKH
GAKLGFMGFFTKACVQALKDIPAVNAEMDGTDLIYKNYYHIGVAVGTDKGLVVPVVRDCDEKSIADIEKSIVDYGKRARD
GQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQERPVVIGGKIEVRPMMYLALSYDHRVIDGKEAVTFL
VRVKESLEDPARLVLDL

Sequences:

>Translated_417_residues
MTEIRVPTLGESVTEATIGRWFKKPGEAVAVDEPLVELETDKVTIEVPAPSAGTLGEIVAKDGETVAVGALLGQITDGAA
PAKPAAAAPAKAAAPAAAPAPAAPAPAAAPAAKAPPSDAPLAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKA
ASAPTPVNQPAAAVQVRGPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRAQYKDVFEKKH
GAKLGFMGFFTKACVQALKDIPAVNAEMDGTDLIYKNYYHIGVAVGTDKGLVVPVVRDCDEKSIADIEKSIVDYGKRARD
GQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQERPVVIGGKIEVRPMMYLALSYDHRVIDGKEAVTFL
VRVKESLEDPARLVLDL
>Mature_416_residues
TEIRVPTLGESVTEATIGRWFKKPGEAVAVDEPLVELETDKVTIEVPAPSAGTLGEIVAKDGETVAVGALLGQITDGAAP
AKPAAAAPAKAAAPAAAPAPAAPAPAAAPAAKAPPSDAPLAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAA
SAPTPVNQPAAAVQVRGPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRAQYKDVFEKKHG
AKLGFMGFFTKACVQALKDIPAVNAEMDGTDLIYKNYYHIGVAVGTDKGLVVPVVRDCDEKSIADIEKSIVDYGKRARDG
QLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQERPVVIGGKIEVRPMMYLALSYDHRVIDGKEAVTFLV
RVKESLEDPARLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=59.4827586206897, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=28.9293849658314, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=29.2576419213974, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI203098816, Length=458, Percent_Identity=29.2576419213974, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=28.0821917808219, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=38.125, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786946, Length=415, Percent_Identity=48.6746987951807, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1786305, Length=428, Percent_Identity=35.7476635514019, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=414, Percent_Identity=42.9951690821256, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=29.8206278026906, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=25.9345794392523, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.3387622149837, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=45, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=26.6075388026608, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=54.2168674698795, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=30.166270783848, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=31.7596566523605, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=31.7596566523605, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43865; Mature: 43734

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIRVPTLGESVTEATIGRWFKKPGEAVAVDEPLVELETDKVTIEVPAPSAGTLGEIVA
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEEECCCCCCCHHHHHH
KDGETVAVGALLGQITDGAAPAKPAAAAPAKAAAPAAAPAPAAPAPAAAPAAKAPPSDAP
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTPVNQPAAAVQVRGPS
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
PADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRAQYKDVFEKKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GAKLGFMGFFTKACVQALKDIPAVNAEMDGTDLIYKNYYHIGVAVGTDKGLVVPVVRDCD
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEEEEEEECCCCEEEEHHHCCC
EKSIADIEKSIVDYGKRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGM
CHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCCCCCCCCH
HKIQERPVVIGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL
HHHCCCCEEECCEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
TEIRVPTLGESVTEATIGRWFKKPGEAVAVDEPLVELETDKVTIEVPAPSAGTLGEIVA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEEECCCCCCCHHHHHH
KDGETVAVGALLGQITDGAAPAKPAAAAPAKAAAPAAAPAPAAPAPAAAPAAKAPPSDAP
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LAPSVRRLSTESGVDASTVPGSGKDGRVTKGDMLAAIEKAASAPTPVNQPAAAVQVRGPS
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCC
PADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTTFNEVDMTNVMALRAQYKDVFEKKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GAKLGFMGFFTKACVQALKDIPAVNAEMDGTDLIYKNYYHIGVAVGTDKGLVVPVVRDCD
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEEEEEEECCCCEEEEHHHCCC
EKSIADIEKSIVDYGKRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGM
CHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCCCCCCCCH
HKIQERPVVIGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL
HHHCCCCEEECCEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]