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The map label for this gene is cheB3

Identifier: 192288573

GI number: 192288573

Start: 155870

End: 156961

Strand: Reverse

Name: cheB3

Synonym: Rpal_0140

Alternate gene names: 192288573

Gene position: 156961-155870 (Counterclockwise)

Preceding gene: 192288574

Following gene: 192288572

Centisome position: 2.73

GC content: 66.48

Gene sequence:

>1092_bases
ATGGCGAAGCAGAAGATTCGAGTCCTGATCGTCGACGACTCCGCCTCGGTGCGACAGGTCCTCGGCACCATTCTGGCCGA
AGACCCCGAGATCGAAGTGATCGGAACCGCGTCCGATCCGTTCGTGGCGGCGAAGCGGCTGCAGAACGAACTTCCCGACG
TCATCCTGCTCGATATCGAGATGCCGCGGATGGACGGCATCACGTTCCTGCGCAAGATCATGGCGCAGCATCCGATCCCG
GTGGTGATCTGCTCGTCACTGACTCCCGAAGGTTCCGACCTGATGTTCGAGGCGCTCGAGGCGGGCGCCGTCGATATCGT
GCCCAAGCCGCGGGTCGACACCCGCCAGGCGCTGATGGAGAGCTCCGGCCGCCTGCGCGAAGCCATCAAGTCGGCAGCCC
GCGCTCGTGTCCGCCCCCGCGCTGCCCGCAAGGTGATTGAACAGAAGCTGACGGCCGATGCGATCATTCCGCCGCCGGTC
GCCGGGCGGTCGCGCCCGACCACCGAGCGGATCGTGTGCATCGGGGTGTCGACCGGCGGCACCGAAGCGCTTTACGACGT
GCTCGAAGTGCTACCGCCGAACTGCCCCGGCATCCTGATCGTGCAGCACATGCCGCAGGGCTTCACCGCGGCATTCGCCA
AGCGGCTCAACGCCGGCTGCCAGATCAACGTCAAGGAAGCCGAGGACGGCGATCCGGTGCTGCCGGGCTGGGCCTATATC
GCTCCCGGCGCCCGGCACATGCTGCTGGTCCGCAGTGGCCTGCGTTATCAGATCGCCATCAAGGACGGCCCGCCGGTGTC
GCGGCACCGTCCCTCGGCCGACGTGCTGTTTCGATCGGCCGCCCAATATGCCGGCGCCAACGCGCTCGGCATCATCATGA
CCGGCATGGGTGACGACGGCGCCCGCGGTCTGCTCGAGATGCGCAAGCTCGGCGCCTACACCTGCGCGCAGGACGAAGAG
AGCTGCGTCGTATTCGGCATGCCGAAGGAAGCGATCGCCTGCGGCGCCGTCGAGAAGGTAGTGTCGCTGAACCAGATCCC
GCGCGAGATCATGGCCTGGCAACAAGCCAAGCAGCCGGCCTCAGCGGATTGA

Upstream 100 bases:

>100_bases
TTGTCGGCCATTCCGAGTCGATGATTCACAGCGCCCTGCCGAAGTTAAGGCAGGTTCAACCAACCGTCTTTAAACTGTAG
GGATGATTGGAGCGCATCAG

Downstream 100 bases:

>100_bases
CGTCGTGTCCTCATTGAAACATCCGATTGCCGACGCCGTTGCGTCGATTGAACTGGTTGCGTCCCGGATCGAGACCACCT
TCGCCCAGGTTGGCGGCCAG

Product: response regulator receiver modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKQKIRVLIVDDSASVRQVLGTILAEDPEIEVIGTASDPFVAAKRLQNELPDVILLDIEMPRMDGITFLRKIMAQHPIP
VVICSSLTPEGSDLMFEALEAGAVDIVPKPRVDTRQALMESSGRLREAIKSAARARVRPRAARKVIEQKLTADAIIPPPV
AGRSRPTTERIVCIGVSTGGTEALYDVLEVLPPNCPGILIVQHMPQGFTAAFAKRLNAGCQINVKEAEDGDPVLPGWAYI
APGARHMLLVRSGLRYQIAIKDGPPVSRHRPSADVLFRSAAQYAGANALGIIMTGMGDDGARGLLEMRKLGAYTCAQDEE
SCVVFGMPKEAIACGAVEKVVSLNQIPREIMAWQQAKQPASAD

Sequences:

>Translated_363_residues
MAKQKIRVLIVDDSASVRQVLGTILAEDPEIEVIGTASDPFVAAKRLQNELPDVILLDIEMPRMDGITFLRKIMAQHPIP
VVICSSLTPEGSDLMFEALEAGAVDIVPKPRVDTRQALMESSGRLREAIKSAARARVRPRAARKVIEQKLTADAIIPPPV
AGRSRPTTERIVCIGVSTGGTEALYDVLEVLPPNCPGILIVQHMPQGFTAAFAKRLNAGCQINVKEAEDGDPVLPGWAYI
APGARHMLLVRSGLRYQIAIKDGPPVSRHRPSADVLFRSAAQYAGANALGIIMTGMGDDGARGLLEMRKLGAYTCAQDEE
SCVVFGMPKEAIACGAVEKVVSLNQIPREIMAWQQAKQPASAD
>Mature_362_residues
AKQKIRVLIVDDSASVRQVLGTILAEDPEIEVIGTASDPFVAAKRLQNELPDVILLDIEMPRMDGITFLRKIMAQHPIPV
VICSSLTPEGSDLMFEALEAGAVDIVPKPRVDTRQALMESSGRLREAIKSAARARVRPRAARKVIEQKLTADAIIPPPVA
GRSRPTTERIVCIGVSTGGTEALYDVLEVLPPNCPGILIVQHMPQGFTAAFAKRLNAGCQINVKEAEDGDPVLPGWAYIA
PGARHMLLVRSGLRYQIAIKDGPPVSRHRPSADVLFRSAAQYAGANALGIIMTGMGDDGARGLLEMRKLGAYTCAQDEES
CVVFGMPKEAIACGAVEKVVSLNQIPREIMAWQQAKQPASAD

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=356, Percent_Identity=51.123595505618, Blast_Score=356, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_RHOPA (P62645)

Other databases:

- EMBL:   BX572593
- RefSeq:   NP_945490.1
- ProteinModelPortal:   P62645
- SMR:   P62645
- GeneID:   2688818
- GenomeReviews:   BX571963_GR
- KEGG:   rpa:RPA0137
- NMPDR:   fig|258594.1.peg.134
- HOGENOM:   HBG705324
- OMA:   PGGKHML
- ProtClustDB:   CLSK818737
- BioCyc:   RPAL258594:RPA0137-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39081; Mature: 38950

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 177-177 ACT_SITE 203-203 ACT_SITE 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKIRVLIVDDSASVRQVLGTILAEDPEIEVIGTASDPFVAAKRLQNELPDVILLDIE
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEC
MPRMDGITFLRKIMAQHPIPVVICSSLTPEGSDLMFEALEAGAVDIVPKPRVDTRQALME
CCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHH
SSGRLREAIKSAARARVRPRAARKVIEQKLTADAIIPPPVAGRSRPTTERIVCIGVSTGG
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
TEALYDVLEVLPPNCPGILIVQHMPQGFTAAFAKRLNAGCQINVKEAEDGDPVLPGWAYI
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEE
APGARHMLLVRSGLRYQIAIKDGPPVSRHRPSADVLFRSAAQYAGANALGIIMTGMGDDG
CCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCH
ARGLLEMRKLGAYTCAQDEESCVVFGMPKEAIACGAVEKVVSLNQIPREIMAWQQAKQPA
HHHHHHHHHHCCEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAD
CCC
>Mature Secondary Structure 
AKQKIRVLIVDDSASVRQVLGTILAEDPEIEVIGTASDPFVAAKRLQNELPDVILLDIE
CCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEC
MPRMDGITFLRKIMAQHPIPVVICSSLTPEGSDLMFEALEAGAVDIVPKPRVDTRQALME
CCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHH
SSGRLREAIKSAARARVRPRAARKVIEQKLTADAIIPPPVAGRSRPTTERIVCIGVSTGG
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
TEALYDVLEVLPPNCPGILIVQHMPQGFTAAFAKRLNAGCQINVKEAEDGDPVLPGWAYI
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEE
APGARHMLLVRSGLRYQIAIKDGPPVSRHRPSADVLFRSAAQYAGANALGIIMTGMGDDG
CCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCH
ARGLLEMRKLGAYTCAQDEESCVVFGMPKEAIACGAVEKVVSLNQIPREIMAWQQAKQPA
HHHHHHHHHHCCEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SAD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA