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The map label for this gene is pheS

Identifier: 192288471

GI number: 192288471

Start: 43710

End: 44792

Strand: Reverse

Name: pheS

Synonym: Rpal_0038

Alternate gene names: 192288471

Gene position: 44792-43710 (Counterclockwise)

Preceding gene: 192288472

Following gene: 192288470

Centisome position: 0.78

GC content: 65.0

Gene sequence:

>1083_bases
ATGTCCAATCTCGAAAACCTGCAATCCCAAATCCTCGCCGACATCGCCGCAGCGGCCGATGAAGCCGCGCTGGAAGCGGT
CCGTGTCGGGGCGCTCGGCAAGAAGGGCTCGATCTCGGCACTGCTCGCCACGCTCGGCAAGATGGACCCCGAGCAGCGCA
AGACCGAGGGCGCGGCGATCAATCGCGCCAAGGAGGCGGTGACCGACGCGCTGACCGCCCGCCGCGACGTGCTGAAGGCC
GCGGCACTCGACGCCAAGCTGGCCGCCGAAACCATCGACGTCACCTTGCCGATGCGCGAGCCCGCCGCCGAGCAGGGCCG
GCTGCATCCGCTCAGCCAGGTGTGGGACGAGCTCACCGCGATCTTCGCCGACATGGGCTTCTCGATCGCCGAAGGTCCGG
ACATCGAGACTGACGACTACAACTTCACCAAGCTCAACTTCCCCGAGGGCCACCCGGCCCGGGAGATGCACGACACCTTC
TATTTCAACCCGAAGGAAGACGGCTCGCGCCTCTTGCTCCGCACCCACACCTCGCCGGTGCAGGTGCGAACCATGCTGTC
GCAGCGGCCGCCGATCCGGGTGATCTGTCCCGGCCGCACCTATCGCAGCGACTCGGACCAGACCCACACGCCGATGTTCC
ATCAGGTCGAAGGGCTGGTGATCGACAAGGGCTCGCACCTCGGCCACCTGAAGTGGATCCTGCACGAGTTCTGCAAGGCG
TTCTTCGAGGTCGACAACGTCAACATGCGGTTTCGGCCGTCGTTCTTCCCGTTCACCGAGCCGTCGCTGGAAGTCGATAT
CCAGTGCCGCCGCGGCAAGGACGAGATCAGGTTCGGCGAAGGCGAGGACTGGCTGGAGATTCTCGGCTGCGGCATGGTGC
ACCCCAACGTGCTCACCGCCTGCGGGCTCGATCCGGACGAGTATCAGGGCTTCGCCTGGGGCATGGGCATCGATCGCATC
GCGATGCTGAAATACGGCATGAGCGATCTGCGGCAGTTGTTCGAAGCCGACGTGCGCTGGCTCAATCACTACGGCTTCAA
GCCGCTCGACATCCCGACGCTGGCGGGCGGATTGAGTTCGTGA

Upstream 100 bases:

>100_bases
TGACCGTCATCGCCGGGCTTGACCCGGCGATCCATCTTTCGAGAAGCGATGGATGCCCGGGTCAAGCCCGGGCATGACTT
CTTGAGGGGAACGGCGGCTC

Downstream 100 bases:

>100_bases
GTCTTGCAGGCGCAGTGTGTGGGGCAAGCGCCCTCACCCCAGCCCTCTCCCGCAAGCGGGAGAGGGGGCAGACTGCGGCT
TGCGGGAGCTCATTGCCTGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSNLENLQSQILADIAAAADEAALEAVRVGALGKKGSISALLATLGKMDPEQRKTEGAAINRAKEAVTDALTARRDVLKA
AALDAKLAAETIDVTLPMREPAAEQGRLHPLSQVWDELTAIFADMGFSIAEGPDIETDDYNFTKLNFPEGHPAREMHDTF
YFNPKEDGSRLLLRTHTSPVQVRTMLSQRPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSHLGHLKWILHEFCKA
FFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTACGLDPDEYQGFAWGMGIDRI
AMLKYGMSDLRQLFEADVRWLNHYGFKPLDIPTLAGGLSS

Sequences:

>Translated_360_residues
MSNLENLQSQILADIAAAADEAALEAVRVGALGKKGSISALLATLGKMDPEQRKTEGAAINRAKEAVTDALTARRDVLKA
AALDAKLAAETIDVTLPMREPAAEQGRLHPLSQVWDELTAIFADMGFSIAEGPDIETDDYNFTKLNFPEGHPAREMHDTF
YFNPKEDGSRLLLRTHTSPVQVRTMLSQRPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSHLGHLKWILHEFCKA
FFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTACGLDPDEYQGFAWGMGIDRI
AMLKYGMSDLRQLFEADVRWLNHYGFKPLDIPTLAGGLSS
>Mature_359_residues
SNLENLQSQILADIAAAADEAALEAVRVGALGKKGSISALLATLGKMDPEQRKTEGAAINRAKEAVTDALTARRDVLKAA
ALDAKLAAETIDVTLPMREPAAEQGRLHPLSQVWDELTAIFADMGFSIAEGPDIETDDYNFTKLNFPEGHPAREMHDTFY
FNPKEDGSRLLLRTHTSPVQVRTMLSQRPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSHLGHLKWILHEFCKAF
FEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTACGLDPDEYQGFAWGMGIDRIA
MLKYGMSDLRQLFEADVRWLNHYGFKPLDIPTLAGGLSS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=274, Percent_Identity=33.2116788321168, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=53.1976744186046, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=32.1167883211679, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=32.1167883211679, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=31.0606060606061, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=271, Percent_Identity=32.4723247232472, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=108, Percent_Identity=35.1851851851852, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI18858079, Length=272, Percent_Identity=31.6176470588235, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_RHOPA (Q6NDR7)

Other databases:

- EMBL:   BX572593
- RefSeq:   NP_945393.1
- ProteinModelPortal:   Q6NDR7
- SMR:   Q6NDR7
- GeneID:   2692030
- GenomeReviews:   BX571963_GR
- KEGG:   rpa:RPA0037
- NMPDR:   fig|258594.1.peg.37
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   RPAL258594:RPA0037-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40115; Mature: 39984

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLENLQSQILADIAAAADEAALEAVRVGALGKKGSISALLATLGKMDPEQRKTEGAAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCHHH
NRAKEAVTDALTARRDVLKAAALDAKLAAETIDVTLPMREPAAEQGRLHPLSQVWDELTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
IFADMGFSIAEGPDIETDDYNFTKLNFPEGHPAREMHDTFYFNPKEDGSRLLLRTHTSPV
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHEECCCCCCCCEEEEEECCCHH
QVRTMLSQRPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSHLGHLKWILHEFCKA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTA
HHHHCCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHC
CGLDPDEYQGFAWGMGIDRIAMLKYGMSDLRQLFEADVRWLNHYGFKPLDIPTLAGGLSS
CCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCC
>Mature Secondary Structure 
SNLENLQSQILADIAAAADEAALEAVRVGALGKKGSISALLATLGKMDPEQRKTEGAAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCHHH
NRAKEAVTDALTARRDVLKAAALDAKLAAETIDVTLPMREPAAEQGRLHPLSQVWDELTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
IFADMGFSIAEGPDIETDDYNFTKLNFPEGHPAREMHDTFYFNPKEDGSRLLLRTHTSPV
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHEECCCCCCCCEEEEEECCCHH
QVRTMLSQRPPIRVICPGRTYRSDSDQTHTPMFHQVEGLVIDKGSHLGHLKWILHEFCKA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FFEVDNVNMRFRPSFFPFTEPSLEVDIQCRRGKDEIRFGEGEDWLEILGCGMVHPNVLTA
HHHHCCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHC
CGLDPDEYQGFAWGMGIDRIAMLKYGMSDLRQLFEADVRWLNHYGFKPLDIPTLAGGLSS
CCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA