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The map label for this gene is rsmB [H]

Identifier: 192288464

GI number: 192288464

Start: 35360

End: 36712

Strand: Reverse

Name: rsmB [H]

Synonym: Rpal_0031

Alternate gene names: 192288464

Gene position: 36712-35360 (Counterclockwise)

Preceding gene: 192288467

Following gene: 192288463

Centisome position: 0.64

GC content: 69.11

Gene sequence:

>1353_bases
ATGCCTGCTCCAAGATTCGCCCAAGCTTCCGAGGTGCCCGGCCTCGCTGCGCGCCGCATTGCCGCGGACATTCTGGACGG
CGTGCTGCAGAAGCGCCGCATGCTCGACGACCAGCTCGAAGGCCCGGCCGCGCATCCGGGTCTGAAGACGCTGTCGGATC
GTGACCGCGCGCTGATGCGCCGCCTGGTCGCGACGATTTTGCGCCGTCTCGGAACGCTGGAAGGGCTGCTCGCCAAGCTG
CTCGATCGCGGCGTGCCGACCGATGCGCCGCGCGCCAAGAGCGCGCTGTTGATCGGCGCCGCGCAAATTCTCTGGATGGA
CGTGCCGGACCATGCCGCCGTCGATCTGTCGGTGCGGCTGGTGCAATCCGACCGTCGCGCTGCCAAATATGCCGGTCTCG
TCAACGCCGTGCTGCGCCGCTGTGCGCGCGAAGGCCAGCCGCTGGTCGAGGAACTGGCGGCCGGAACGCCCGACGTGCCG
CAATGGCTTTTGGCGCGCTGGAGCGAACATTACGGTGCCGAGACCGCGCGTGCGATCGCGCACGCGATCAGCCACGAACC
CTCACTCGACCTCACGGTGAAATCCGACCCGGCGCACTGGGCGGCGCGGCTGCACGGCGAAGTGCTGCCGACCGGCACCG
TGCGCACGCTGTTGCAGGGCTCGGTGCGGATGCTGCCGGGCTTTGATGAAGGCCAGTGGTGGGTACAGGACGCGGCCGCC
GCGCTGCCGGCACGGCTGTTCGGCGATATCGCCGGCAAGAGCGTCGCCGATCTTTGCGCGGCCCCTGGCGGCAAGACTGC
ACAGCTCGCCGCCGCCGGCGCCAGGGTCACTGCGATCGACCGTTCGCCCGCTCGGGTCGCGCGATTGCGCGAAAACCTGG
CGCGGCTGTCGTTTGAGGTCGAAACCGGCGTCGCCGATGCGGCCGAATGGCGAGGCGATGAGACCGCCGAGTTCGACGCG
GTGCTGATCGATGCGCCCTGCGCCTCGACCGGCACGATCCGGCGGCATCCCGACGTCGCCTGGCTGAAGCAGGAGAGCGA
CATCGGCGCGCTCGCTGCGGTTCAGAATCGGCTGTTGAAGAAGGCGGCGTCATTGCTGCGACCCGGCGGTACGTTGGTGT
ATTGCACCTGTTCGCTCGAGCCCGAGGAGGGCGAGCAGGCGATCGCCGCGCTGCTCGACGCCGAGTCGTCGATGCGCCGG
CAGCCGATCGATCCGGCAGAAGTCGCCGGTCTCGCCGAGATCGTCACCGCAGATGGTGCGCTGCGGACCCTACCGTGCCA
TCTGCCGCACAGCGATCCGCGGCTCGCCGGGCTCGACGGCTTCTATGCCGCGCGGCTGGTGAAGTCGTTGTAA

Upstream 100 bases:

>100_bases
GACGGCGATTCTTACATTTGGCATATTCGAATGCCATCCACGGCAGACCAGCGCGCGGCCTCGCGCGGCGTCTCTCCTCC
TGCTGTGATACCCTCAATCC

Downstream 100 bases:

>100_bases
TTCTTCAAGTTTGATCGGCTGTGGGGCGTGGCGCAGCAGCGCCCGTCCACATTCGCCTATCGCCAGGGTGTGTGAACGCC
AATTCCAGATTAAAAGGATT

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPAPRFAQASEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLSDRDRALMRRLVATILRRLGTLEGLLAKL
LDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQPLVEELAAGTPDVP
QWLLARWSEHYGAETARAIAHAISHEPSLDLTVKSDPAHWAARLHGEVLPTGTVRTLLQGSVRMLPGFDEGQWWVQDAAA
ALPARLFGDIAGKSVADLCAAPGGKTAQLAAAGARVTAIDRSPARVARLRENLARLSFEVETGVADAAEWRGDETAEFDA
VLIDAPCASTGTIRRHPDVAWLKQESDIGALAAVQNRLLKKAASLLRPGGTLVYCTCSLEPEEGEQAIAALLDAESSMRR
QPIDPAEVAGLAEIVTADGALRTLPCHLPHSDPRLAGLDGFYAARLVKSL

Sequences:

>Translated_450_residues
MPAPRFAQASEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLSDRDRALMRRLVATILRRLGTLEGLLAKL
LDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQPLVEELAAGTPDVP
QWLLARWSEHYGAETARAIAHAISHEPSLDLTVKSDPAHWAARLHGEVLPTGTVRTLLQGSVRMLPGFDEGQWWVQDAAA
ALPARLFGDIAGKSVADLCAAPGGKTAQLAAAGARVTAIDRSPARVARLRENLARLSFEVETGVADAAEWRGDETAEFDA
VLIDAPCASTGTIRRHPDVAWLKQESDIGALAAVQNRLLKKAASLLRPGGTLVYCTCSLEPEEGEQAIAALLDAESSMRR
QPIDPAEVAGLAEIVTADGALRTLPCHLPHSDPRLAGLDGFYAARLVKSL
>Mature_449_residues
PAPRFAQASEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLSDRDRALMRRLVATILRRLGTLEGLLAKLL
DRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRLVQSDRRAAKYAGLVNAVLRRCAREGQPLVEELAAGTPDVPQ
WLLARWSEHYGAETARAIAHAISHEPSLDLTVKSDPAHWAARLHGEVLPTGTVRTLLQGSVRMLPGFDEGQWWVQDAAAA
LPARLFGDIAGKSVADLCAAPGGKTAQLAAAGARVTAIDRSPARVARLRENLARLSFEVETGVADAAEWRGDETAEFDAV
LIDAPCASTGTIRRHPDVAWLKQESDIGALAAVQNRLLKKAASLLRPGGTLVYCTCSLEPEEGEQAIAALLDAESSMRRQ
PIDPAEVAGLAEIVTADGALRTLPCHLPHSDPRLAGLDGFYAARLVKSL

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=242, Percent_Identity=30.9917355371901, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI76150623, Length=242, Percent_Identity=30.9917355371901, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI32698918, Length=203, Percent_Identity=29.064039408867, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI40316918, Length=231, Percent_Identity=30.7359307359307, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI11545785, Length=203, Percent_Identity=29.5566502463054, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI2367212, Length=455, Percent_Identity=31.8681318681319, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI87081985, Length=257, Percent_Identity=31.9066147859922, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=239, Percent_Identity=29.7071129707113, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=29.6943231441048, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71998419, Length=229, Percent_Identity=29.6943231441048, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509941, Length=177, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=241, Percent_Identity=29.4605809128631, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319447, Length=177, Percent_Identity=32.7683615819209, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI22024126, Length=237, Percent_Identity=29.535864978903, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355201, Length=186, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24668781, Length=173, Percent_Identity=32.3699421965318, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356513, Length=173, Percent_Identity=32.3699421965318, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48085; Mature: 47953

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPRFAQASEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLSDRDRALMR
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RLVATILRRLGTLEGLLAKLLDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEHHHHEE
VQSDRRAAKYAGLVNAVLRRCAREGQPLVEELAAGTPDVPQWLLARWSEHYGAETARAIA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHH
HAISHEPSLDLTVKSDPAHWAARLHGEVLPTGTVRTLLQGSVRMLPGFDEGQWWVQDAAA
HHHCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
ALPARLFGDIAGKSVADLCAAPGGKTAQLAAAGARVTAIDRSPARVARLRENLARLSFEV
HHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHEEHH
ETGVADAAEWRGDETAEFDAVLIDAPCASTGTIRRHPDVAWLKQESDIGALAAVQNRLLK
HCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHH
KAASLLRPGGTLVYCTCSLEPEEGEQAIAALLDAESSMRRQPIDPAEVAGLAEIVTADGA
HHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
LRTLPCHLPHSDPRLAGLDGFYAARLVKSL
CEEEECCCCCCCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure 
PAPRFAQASEVPGLAARRIAADILDGVLQKRRMLDDQLEGPAAHPGLKTLSDRDRALMR
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RLVATILRRLGTLEGLLAKLLDRGVPTDAPRAKSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEHHHHEE
VQSDRRAAKYAGLVNAVLRRCAREGQPLVEELAAGTPDVPQWLLARWSEHYGAETARAIA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHH
HAISHEPSLDLTVKSDPAHWAARLHGEVLPTGTVRTLLQGSVRMLPGFDEGQWWVQDAAA
HHHCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
ALPARLFGDIAGKSVADLCAAPGGKTAQLAAAGARVTAIDRSPARVARLRENLARLSFEV
HHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHEEHH
ETGVADAAEWRGDETAEFDAVLIDAPCASTGTIRRHPDVAWLKQESDIGALAAVQNRLLK
HCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHHHH
KAASLLRPGGTLVYCTCSLEPEEGEQAIAALLDAESSMRRQPIDPAEVAGLAEIVTADGA
HHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
LRTLPCHLPHSDPRLAGLDGFYAARLVKSL
CEEEECCCCCCCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA