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The map label for this gene is stcD [H]

Identifier: 190894924

GI number: 190894924

Start: 641243

End: 643279

Strand: Reverse

Name: stcD [H]

Synonym: RHECIAT_PC0000589

Alternate gene names: 190894924

Gene position: 643279-641243 (Counterclockwise)

Preceding gene: 190894931

Following gene: 190894923

Centisome position: 58.93

GC content: 61.41

Gene sequence:

>2037_bases
ATGTCGAATGATCCTCTCCTTCAGCCCTACCAGCTCAAGCATCTGAAGCTGCGCAACCGTATCATTGTGACCTCGCACGA
GCCGGCCTATCCGGAGGACGGTATGCCGAAGGGAAAGTATCGCGCCTATACGGTGGAGAGGGCAAAGGGCGGCGTCGCCC
TGACGATGACGGCGGGTTCGGCGGCCGTCTCCAAGGACAGCCCGCCGGTCTTCAACAACCTGCTCGCCTATAAGGACGAG
ATCGTTCCCTGGATCAGGGAAATGACCGATGCGGTGCACGAAGAGGGGGCGGCGATCATGATCCAGCTCACCCATCTCGG
CCGGCGCACGCGCTGGGACAAGGGCGACTGGCTGCCGGTTGTGGCGCCGTCGCATCATCGCGAGGCTTCGCACCGCGCCT
TCCCGAAGAAGTTGGAAGACTGGGATATCGAGCGGATCATCAAGGATTTCGCCGATGCGGCCGAGCGCATGAAGGCGGGC
GGCATGGACGGCGTCGAGCTTGAGGCCTACGGCCACCTGATCGATCAGTTCGTGTCTCCGCTCACCAATGAACTCGATGG
CCCCTATGGCGGTTCGCTCGACAACCGTCTGCGCTTCTGTTTCGACGTGTTCAAGGCGATCCGGAAACGGGTGGGCGACG
AGTTCATTCTCGGCCTGCGCTATACGGCCGACGAATGCCTTCCCGGCGGCACGGGCAAGGCCGAAGGGCTCGAAATCTCC
AACCGGTTGAAAGAGAGCGGCCTCATCGATTACCTGAATGTCATCCGTGGACACATCGATACCGATGCCGGTTTGACAGA
CGTGATCCCGATCCAGGGCATGGCGAATTCCCCGCATCTCGATTTCGCCGGCGAAATCCGCGCCGCCACCCAATTTCCGA
CCTTCCATGCGGCGAAGATCCCCGACGTCGCAACCGCGCGCCACGCGATTGCGGCCGGCAAGGTCGACATGGTCGGCATG
ACCCGCGCCCACATGACCGACCCGCATATTGTCCGCAAGATCATCGAGAAACGGGAAGAGGATATCCGCCCCTGCGTCGG
CGCCAACTACTGTCTCGACCGCATCTATCAGGGCGGCGCCGCCTATTGCATCCACAATGCCGCCACCGGCCGCGAACTGA
CCATGCCGCATATCCTTGATAAGGCCGATGTGAAGAAGAAGGTCGTTATCGTCGGCGCCGGTCCGGCCGGTCTCGAGGCG
GCGCGGGTTGCTGGGGAACGCGGCCACAAGGTGGTCGTTTTCGAAGCGGCGAACAATCCCGGCGGCCAAATCCGCCTCAC
CGCCCAGAGCGAACGCCGCAGGGAGATGATCAGCATCATCGACTGGCGCATGAGCCAGTGCGAAAAATACGACGTGACCT
TCCACTTCAACAGCTGGGCGGAAGCCGACACGATCGAAGCCGAAAACCCCGATGTCGTCATTATCGCGACCGGCGGCCTG
CCGCATACCGAGGTGCTTTCGACGGGCAACGAGCTGGTGGTCTCCTCATGGGACATCATCTCCGGGGACGTGAAGCCTGG
CAGCAACGTGCTGATTTTCGACGATGCCGGCGACCATGCCGGCCTTCAGGCGGCGGAGTTTCTCGCTAAGGCGGGCGCCA
AGGTCGAGATCATGACGCCCGACCGGTCCTTCGCGCCTGAGGTCATGGCGATGAACCTTGTGCCCTATATGCGCTCCCTT
CAGAAGCATGACGTGACCTTCACCGTCACCTATCGCCTGGAAGCGGTCGAGAAGAGCGGCAATCAGCTTGTTGCCCATGT
CGGCAGCGATTACGGCGGGATTGCCAAGCAGAGCAGCTACGACCAGATCGTCGTCAATCACGGGACCATTCCGCTCGATG
AGCTCTATTTCGAGCTGAAGCCCAATTCGAGCAACCTCGGCGAGATGTCGTATGACCAGCTTCTGGCCGGTGAACCGCAG
TCGGTCATTCGCAATCCCGAGGGCAAATTCCAGCTGTTCCGGATCGGCGATGCTGTCGCTGCGCGCAACACGCATGCCGC
CGTCTATGACGGCCTGCGCATCGCGAAGGATATATGA

Upstream 100 bases:

>100_bases
CATATGTGTCAAAAATGGCGACTTATCTGTTTTCCAAGTTGACACATATGTACATTGCGCATAGCGTTCCCTTGTTGTTT
TTAATCCGGAACCGCGGCCC

Downstream 100 bases:

>100_bases
TTGCAGCAGAGGCCACCGGCTGGCAGGCCTAAAATCGTCCTGTTCTAAAGCGTGTCGCGATCTTTCGGATTCGCTTGTAA
CGCTTTAGGTCTTTGTTTTT

Product: 2,4-dienoyl-CoA reductase (NADPH) protein

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSNDPLLQPYQLKHLKLRNRIIVTSHEPAYPEDGMPKGKYRAYTVERAKGGVALTMTAGSAAVSKDSPPVFNNLLAYKDE
IVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRAFPKKLEDWDIERIIKDFADAAERMKAG
GMDGVELEAYGHLIDQFVSPLTNELDGPYGGSLDNRLRFCFDVFKAIRKRVGDEFILGLRYTADECLPGGTGKAEGLEIS
NRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMANSPHLDFAGEIRAATQFPTFHAAKIPDVATARHAIAAGKVDMVGM
TRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGAAYCIHNAATGRELTMPHILDKADVKKKVVIVGAGPAGLEA
ARVAGERGHKVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNSWAEADTIEAENPDVVIIATGGL
PHTEVLSTGNELVVSSWDIISGDVKPGSNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTPDRSFAPEVMAMNLVPYMRSL
QKHDVTFTVTYRLEAVEKSGNQLVAHVGSDYGGIAKQSSYDQIVVNHGTIPLDELYFELKPNSSNLGEMSYDQLLAGEPQ
SVIRNPEGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI

Sequences:

>Translated_678_residues
MSNDPLLQPYQLKHLKLRNRIIVTSHEPAYPEDGMPKGKYRAYTVERAKGGVALTMTAGSAAVSKDSPPVFNNLLAYKDE
IVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRAFPKKLEDWDIERIIKDFADAAERMKAG
GMDGVELEAYGHLIDQFVSPLTNELDGPYGGSLDNRLRFCFDVFKAIRKRVGDEFILGLRYTADECLPGGTGKAEGLEIS
NRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMANSPHLDFAGEIRAATQFPTFHAAKIPDVATARHAIAAGKVDMVGM
TRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGAAYCIHNAATGRELTMPHILDKADVKKKVVIVGAGPAGLEA
ARVAGERGHKVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNSWAEADTIEAENPDVVIIATGGL
PHTEVLSTGNELVVSSWDIISGDVKPGSNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTPDRSFAPEVMAMNLVPYMRSL
QKHDVTFTVTYRLEAVEKSGNQLVAHVGSDYGGIAKQSSYDQIVVNHGTIPLDELYFELKPNSSNLGEMSYDQLLAGEPQ
SVIRNPEGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI
>Mature_677_residues
SNDPLLQPYQLKHLKLRNRIIVTSHEPAYPEDGMPKGKYRAYTVERAKGGVALTMTAGSAAVSKDSPPVFNNLLAYKDEI
VPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRAFPKKLEDWDIERIIKDFADAAERMKAGG
MDGVELEAYGHLIDQFVSPLTNELDGPYGGSLDNRLRFCFDVFKAIRKRVGDEFILGLRYTADECLPGGTGKAEGLEISN
RLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMANSPHLDFAGEIRAATQFPTFHAAKIPDVATARHAIAAGKVDMVGMT
RAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGAAYCIHNAATGRELTMPHILDKADVKKKVVIVGAGPAGLEAA
RVAGERGHKVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNSWAEADTIEAENPDVVIIATGGLP
HTEVLSTGNELVVSSWDIISGDVKPGSNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTPDRSFAPEVMAMNLVPYMRSLQ
KHDVTFTVTYRLEAVEKSGNQLVAHVGSDYGGIAKQSSYDQIVVNHGTIPLDELYFELKPNSSNLGEMSYDQLLAGEPQS
VIRNPEGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=542, Percent_Identity=29.520295202952, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1787939, Length=388, Percent_Identity=26.2886597938144, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565138, Length=306, Percent_Identity=27.1241830065359, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=27.3062730627306, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=264, Percent_Identity=26.5151515151515, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17564188, Length=269, Percent_Identity=25.2788104089219, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI72001454, Length=261, Percent_Identity=24.5210727969349, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17566914, Length=235, Percent_Identity=23.8297872340426, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=357, Percent_Identity=24.3697478991597, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=359, Percent_Identity=22.5626740947075, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74591; Mature: 74459

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDPLLQPYQLKHLKLRNRIIVTSHEPAYPEDGMPKGKYRAYTVERAKGGVALTMTAGS
CCCCCCCCCHHHHHEEECCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCC
AAVSKDSPPVFNNLLAYKDEIVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEE
VAPSHHREASHRAFPKKLEDWDIERIIKDFADAAERMKAGGMDGVELEAYGHLIDQFVSP
ECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
LTNELDGPYGGSLDNRLRFCFDVFKAIRKRVGDEFILGLRYTADECLPGGTGKAEGLEIS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHCCCCCCCCCCCCCHH
NRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMANSPHLDFAGEIRAATQFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEHCCCCCCCCCCC
PDVATARHAIAAGKVDMVGMTRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGA
CCHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
AYCIHNAATGRELTMPHILDKADVKKKVVIVGAGPAGLEAARVAGERGHKVVVFEAANNP
EEEEECCCCCCCCCCHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCC
GGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNSWAEADTIEAENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCC
PHTEVLSTGNELVVSSWDIISGDVKPGSNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTP
CHHHHHHCCCEEEEEECCEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECC
DRSFAPEVMAMNLVPYMRSLQKHDVTFTVTYRLEAVEKSGNQLVAHVGSDYGGIAKQSSY
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCC
DQIVVNHGTIPLDELYFELKPNSSNLGEMSYDQLLAGEPQSVIRNPEGKFQLFRIGDAVA
CEEEEECCCCCHHHEEEEECCCCCCCCCCCHHHHHCCCCHHHHCCCCCCEEEEEECCHHH
ARNTHAAVYDGLRIAKDI
CCCCCHHHHHCCHHHCCC
>Mature Secondary Structure 
SNDPLLQPYQLKHLKLRNRIIVTSHEPAYPEDGMPKGKYRAYTVERAKGGVALTMTAGS
CCCCCCCCHHHHHEEECCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCC
AAVSKDSPPVFNNLLAYKDEIVPWIREMTDAVHEEGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEE
VAPSHHREASHRAFPKKLEDWDIERIIKDFADAAERMKAGGMDGVELEAYGHLIDQFVSP
ECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
LTNELDGPYGGSLDNRLRFCFDVFKAIRKRVGDEFILGLRYTADECLPGGTGKAEGLEIS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHCCCCCCCCCCCCCHH
NRLKESGLIDYLNVIRGHIDTDAGLTDVIPIQGMANSPHLDFAGEIRAATQFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEHCCCCCCCCCCC
PDVATARHAIAAGKVDMVGMTRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGA
CCHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
AYCIHNAATGRELTMPHILDKADVKKKVVIVGAGPAGLEAARVAGERGHKVVVFEAANNP
EEEEECCCCCCCCCCHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCC
GGQIRLTAQSERRREMISIIDWRMSQCEKYDVTFHFNSWAEADTIEAENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCC
PHTEVLSTGNELVVSSWDIISGDVKPGSNVLIFDDAGDHAGLQAAEFLAKAGAKVEIMTP
CHHHHHHCCCEEEEEECCEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECC
DRSFAPEVMAMNLVPYMRSLQKHDVTFTVTYRLEAVEKSGNQLVAHVGSDYGGIAKQSSY
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCC
DQIVVNHGTIPLDELYFELKPNSSNLGEMSYDQLLAGEPQSVIRNPEGKFQLFRIGDAVA
CEEEEECCCCCHHHEEEEECCCCCCCCCCCHHHHHCCCCHHHHCCCCCCEEEEEECCHHH
ARNTHAAVYDGLRIAKDI
CCCCCHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]