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The map label for this gene is pgl [H]

Identifier: 190894826

GI number: 190894826

Start: 532595

End: 533959

Strand: Reverse

Name: pgl [H]

Synonym: RHECIAT_PC0000491

Alternate gene names: 190894826

Gene position: 533959-532595 (Counterclockwise)

Preceding gene: 190894827

Following gene: 190894825

Centisome position: 48.92

GC content: 64.32

Gene sequence:

>1365_bases
ATGAGTGCCACCTCCCTCGTCGCGATTGAGGCCTTCGACGGCGACAATACCGATCGCCTGCAGGCGGCGATCGACGATCT
CTCGGCTTCCGGCGGCGGACGCCTGAAGCTCCTGGCGGGCATCCACCTCTGCCGGGGGCTCCAGCTCCGTTCGGGCGTCG
AGCTGCATCTGGCCGCCGGCGCAATCCTGCGCCCTGTTTCCGACTATGAGGCTTACGCGCATACGACTGTTTCGGTAATC
GCCGAGAAGTCGGACCGCGGCATGATCGTCGCCAAGGGCGCACGGCGGATCAGCCTGACGGGACCGGGGCGCATCGAGGC
CGGCTGCGAGAGCTTCATCATCGGCGACGACGAGACGGTGGGGACCTTCATCCCGGCGGAATTCCGCCCCCGCGTCGTCG
TCTTCGAAAGCTGCGACGAGGTCGAGATCAGCGCCGTCCATATCAGCCGCTCGCCGATGTGGACGCTGCACTTCGTCAAC
TGCACCGATGTCGCGGTCCGGAACGTCACCATCGAAAACGACCGCCGTCTTCCCAACACGGATGGCATCGTGTTCGATGC
CTGCCGCGGCGCCGTCATCGAGGATTGCACGATATCGACGGCCGATGACGGCATATGCCTGAAGACCAGCATCGGCCCGC
AGGGTGTCGCCATCGGGCGATGCGAGAATATTCTTATCCGCCGCTGCTCCGTTCAGAGCCTCAGCTGCGCGCTGAAAATC
GGCACTGAAACGCATGGCGACGTCACCAACGTCGTCTTCGAGGATTGCAGCGTCTCATCCTCCAACCGGGCGCTCGGCAT
ATTTTCGCGTGACGGCGGCCGGATCTCGAACGTGAGGTTTTCCAGGATTGCGGTAGAGTGCCGCGAAACGCTCGATGGCT
TCTGGGGCTCGGGGGAGGCGCTGACCGTCAACCTCGTCGACCGTGTCACTGAGCGCCCGGCAGGTGCGATCGAAAACCTC
ATTGTCGAGGACATTACCGGGCGCATGGAGGGGGCGATCACCATCATCTCGACTGCGTCCGCTGGCATCCACAACATATC
GCTGGCACGCATCGGCATAGACCAGTGGCCCGGCGAACTCGGCACCGCTCAGTCCTACGACCTGCGCCCGACCAATGCCG
ATCTCGCGCCGAAGGCCGAGGGCGGCGGCCGCGCCAATGCCTGGACGCGCGGGGCGGACGGCCGGGTGATCGGCCTGCAG
GACTATCCAGGCGGAATGCCCGGCATGTATGTGGCTGATGTCACCGGGATCTTGATGAACGAGGTGCGGATCACAAGACC
GACACCGCTGCCGCAAGGCTGGAACGCAAACGACGTCGTCTTCGAGACGGCGGCACCCGATGGGAGTAGGGCATGGCAGA
ACTGA

Upstream 100 bases:

>100_bases
TTGATTTCTATCGGCGCATCCCGAACCTGCCGACGCCCTACGGCCAGGCACTGACCATGCTGCTGCTGACCGAAGTCCTT
ATCGAGAATGGCCGACGCCA

Downstream 100 bases:

>100_bases
GACTTTCCACCGTCAACAAGTCGTATGGCTCCGTTAAGGTGCTGCATGACGTCGAACTCGATATCAAGGACGGCGAGTTC
GTCGTCTTCGTCGGCCCGTC

Product: putative polygalacturonase

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSATSLVAIEAFDGDNTDRLQAAIDDLSASGGGRLKLLAGIHLCRGLQLRSGVELHLAAGAILRPVSDYEAYAHTTVSVI
AEKSDRGMIVAKGARRISLTGPGRIEAGCESFIIGDDETVGTFIPAEFRPRVVVFESCDEVEISAVHISRSPMWTLHFVN
CTDVAVRNVTIENDRRLPNTDGIVFDACRGAVIEDCTISTADDGICLKTSIGPQGVAIGRCENILIRRCSVQSLSCALKI
GTETHGDVTNVVFEDCSVSSSNRALGIFSRDGGRISNVRFSRIAVECRETLDGFWGSGEALTVNLVDRVTERPAGAIENL
IVEDITGRMEGAITIISTASAGIHNISLARIGIDQWPGELGTAQSYDLRPTNADLAPKAEGGGRANAWTRGADGRVIGLQ
DYPGGMPGMYVADVTGILMNEVRITRPTPLPQGWNANDVVFETAAPDGSRAWQN

Sequences:

>Translated_454_residues
MSATSLVAIEAFDGDNTDRLQAAIDDLSASGGGRLKLLAGIHLCRGLQLRSGVELHLAAGAILRPVSDYEAYAHTTVSVI
AEKSDRGMIVAKGARRISLTGPGRIEAGCESFIIGDDETVGTFIPAEFRPRVVVFESCDEVEISAVHISRSPMWTLHFVN
CTDVAVRNVTIENDRRLPNTDGIVFDACRGAVIEDCTISTADDGICLKTSIGPQGVAIGRCENILIRRCSVQSLSCALKI
GTETHGDVTNVVFEDCSVSSSNRALGIFSRDGGRISNVRFSRIAVECRETLDGFWGSGEALTVNLVDRVTERPAGAIENL
IVEDITGRMEGAITIISTASAGIHNISLARIGIDQWPGELGTAQSYDLRPTNADLAPKAEGGGRANAWTRGADGRVIGLQ
DYPGGMPGMYVADVTGILMNEVRITRPTPLPQGWNANDVVFETAAPDGSRAWQN
>Mature_453_residues
SATSLVAIEAFDGDNTDRLQAAIDDLSASGGGRLKLLAGIHLCRGLQLRSGVELHLAAGAILRPVSDYEAYAHTTVSVIA
EKSDRGMIVAKGARRISLTGPGRIEAGCESFIIGDDETVGTFIPAEFRPRVVVFESCDEVEISAVHISRSPMWTLHFVNC
TDVAVRNVTIENDRRLPNTDGIVFDACRGAVIEDCTISTADDGICLKTSIGPQGVAIGRCENILIRRCSVQSLSCALKIG
TETHGDVTNVVFEDCSVSSSNRALGIFSRDGGRISNVRFSRIAVECRETLDGFWGSGEALTVNLVDRVTERPAGAIENLI
VEDITGRMEGAITIISTASAGIHNISLARIGIDQWPGELGTAQSYDLRPTNADLAPKAEGGGRANAWTRGADGRVIGLQD
YPGGMPGMYVADVTGILMNEVRITRPTPLPQGWNANDVVFETAAPDGSRAWQN

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 48384; Mature: 48253

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSLVAIEAFDGDNTDRLQAAIDDLSASGGGRLKLLAGIHLCRGLQLRSGVELHLAAG
CCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEECC
AILRPVSDYEAYAHTTVSVIAEKSDRGMIVAKGARRISLTGPGRIEAGCESFIIGDDETV
HHHCCCHHHHHHHHEEEEEEEECCCCCEEEECCCEEEEECCCCCCCCCCCEEEECCCCCC
GTFIPAEFRPRVVVFESCDEVEISAVHISRSPMWTLHFVNCTDVAVRNVTIENDRRLPNT
CEECCCCCCCCEEEEECCCCEEEEEEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCC
DGIVFDACRGAVIEDCTISTADDGICLKTSIGPQGVAIGRCENILIRRCSVQSLSCALKI
CCEEEECCCCCEEECCEEECCCCCEEEEECCCCCCEEEECHHEEHHEECCCCEEEEEEEE
GTETHGDVTNVVFEDCSVSSSNRALGIFSRDGGRISNVRFSRIAVECRETLDGFWGSGEA
CCCCCCCHHEEEEECCCCCCCCCEEEEEECCCCEEECEEEEHHHHHHHHHHCCCCCCCCE
LTVNLVDRVTERPAGAIENLIVEDITGRMEGAITIISTASAGIHNISLARIGIDQWPGEL
EEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECHHHCCCCC
GTAQSYDLRPTNADLAPKAEGGGRANAWTRGADGRVIGLQDYPGGMPGMYVADVTGILMN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHC
EVRITRPTPLPQGWNANDVVFETAAPDGSRAWQN
CEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SATSLVAIEAFDGDNTDRLQAAIDDLSASGGGRLKLLAGIHLCRGLQLRSGVELHLAAG
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEECC
AILRPVSDYEAYAHTTVSVIAEKSDRGMIVAKGARRISLTGPGRIEAGCESFIIGDDETV
HHHCCCHHHHHHHHEEEEEEEECCCCCEEEECCCEEEEECCCCCCCCCCCEEEECCCCCC
GTFIPAEFRPRVVVFESCDEVEISAVHISRSPMWTLHFVNCTDVAVRNVTIENDRRLPNT
CEECCCCCCCCEEEEECCCCEEEEEEEEECCCEEEEEEECCCCEEEEEEEEECCCCCCCC
DGIVFDACRGAVIEDCTISTADDGICLKTSIGPQGVAIGRCENILIRRCSVQSLSCALKI
CCEEEECCCCCEEECCEEECCCCCEEEEECCCCCCEEEECHHEEHHEECCCCEEEEEEEE
GTETHGDVTNVVFEDCSVSSSNRALGIFSRDGGRISNVRFSRIAVECRETLDGFWGSGEA
CCCCCCCHHEEEEECCCCCCCCCEEEEEECCCCEEECEEEEHHHHHHHHHHCCCCCCCCE
LTVNLVDRVTERPAGAIENLIVEDITGRMEGAITIISTASAGIHNISLARIGIDQWPGEL
EEEEEHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECHHHCCCCC
GTAQSYDLRPTNADLAPKAEGGGRANAWTRGADGRVIGLQDYPGGMPGMYVADVTGILMN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHC
EVRITRPTPLPQGWNANDVVFETAAPDGSRAWQN
CEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]