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The map label for this gene is rmrB [H]

Identifier: 190894678

GI number: 190894678

Start: 367197

End: 368780

Strand: Reverse

Name: rmrB [H]

Synonym: RHECIAT_PC0000341

Alternate gene names: 190894678

Gene position: 368780-367197 (Counterclockwise)

Preceding gene: 190894679

Following gene: 190894675

Centisome position: 33.79

GC content: 62.31

Gene sequence:

>1584_bases
ATGTCAAGCATTGCGGCCACCGCTGGGGCCATTGCGCAACCGCGCGCCAGCACAAGGGATTGGATCGCCGTCCTCGCCGG
CATGATCGGCGCCTTCATGGCGATTCTCAACATCCAGATCACCAATGCCTCCCTGCTCGATATCGAGGGCGGCATCGGAA
CGGGCGTCGACAACGGCGCCTGGATCTCGACCTCCTACCTGATCGGCGAGATCGTCGTCATTCCGCTGACGGCCTATTTC
AGCAGCGTCTTCTCGTTTCGCCGCTATATCCTCGTCAACTCCATCCTCTTTCCGCTCTTTTCGATCGCCTGCGCCTTCGC
GCATGATCTCGGCACGATGATCCTGCTGCGCGGCCTGCAGGGTTTTGCCGGCGGCGTGCTGATCCCCATGGCCTTCACCA
TGGTGCTGACGAAGCTGCCGAAGAGCCAGCAGCCGCTCGGTCTGGCGATCTTCGCGCTTTCGGTCACCTTCGCGCCGGCG
ATCGGGCCGACCATTGGCGGTTATCTCACCGAAAACTACGGCTGGCAGACGATCTTCTTCATCAATGCCATCCCGAGCGC
GGTGATGGCTGCGGCTCTTGCCCTGACGCTGGAGAAGCAGCCGATGCAGCTTGGCCTCCTTAAAGAGGGCGATTGGGCTG
GTATCGTCACGATGGCGATCGGTCTCTCGGCGCTGCAGACCGTGCTGGAGGAAGGCAACAAGGAAGACTGGTTCGCCTCG
TCTTTCATTGTCAAGCTCAGCATCCTCGCCTTCGTCTTCCTTGCCGCCTTCATCTGGATCGAGCTCACCGTCAAGAAGCC
GCTGGTCAAGCTCAGCCTGCTGACGCAGCGCAATTTCGGCATCGGCGTTGCCGTCAACGTGCTGGTCGGTGTCGCACTCT
TCGGGACCGTCTATATCCTGCCGCAATATCTCGGCCAGGTGCAGCGCTACAATGCCGAGCAGATCGGCAACGTGCTGGCC
TGGACCGGCCTGCCGCAACTGCTGCTGATCCCGCTCGTGCCGATGATGATGAAGCGCTTCGACGCCCGTTATATCGGCTT
CCTCGGCATCTCGATCTTTGCGATCAGCTGCTTCCTCAACATCACGCTTTCGGCCGACACCGCCGGCGACCAATTCTGGA
TTCCGAATATCGTGCGCGCCGTCGGTCAGGCTTTGGTGTTGACGCCGATCACCGCCATCACCACCGCCGGCATCGCGCCA
GCCGATGCGGCCGCCGCCTCGGGGCTGACCAACATGCTGCGCAATCTTGGCGGCGCCGTCGGCACGGCAACGCTCGGCAC
CGTGCTGACCAAACGCGAGCAGTTTCATTCCAATATCATCGGCCAGTCCGTAACGCTCGGCCGGGATGAAGTCCGCGATC
GTCTTGCACAAACGACCGCCTATTTCATGCAGCACGGGGTTTCCGATCCCGCTGTCGCCGCGCACAAGGCCGTTGCCGCT
CTCGGTCAGATCGTCAGGCGCCAGGCCCTCATCCTGGGCTTTGCCGATACTTTCGCCGTGATCGGCGTAGTGCTGGCCAT
TGCTGCCGTCGCCTTGCTGCTCACCCAGAAGCCACGGGCTGGCGCCGGCGCCGGCGCCCACTGA

Upstream 100 bases:

>100_bases
AGCCGCCAATGGCGATCTGCGCCCCGGCATGTCCGTCCAACCGAGCATCGACACCAAGGCCGATGCCGATCGCAAATAAG
CGCAGGATCGGGAGATTGTC

Downstream 100 bases:

>100_bases
CAATCGGCCGCGGCTAACGCTGCAGAAACAACTGTTCTGGATAAAAACTGCTTCGCGCGCCGACAGGGCGCGCGAAGCCT
TTACCGCAAGATCTCGACGG

Product: multidrug efflux transporter protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MSSIAATAGAIAQPRASTRDWIAVLAGMIGAFMAILNIQITNASLLDIEGGIGTGVDNGAWISTSYLIGEIVVIPLTAYF
SSVFSFRRYILVNSILFPLFSIACAFAHDLGTMILLRGLQGFAGGVLIPMAFTMVLTKLPKSQQPLGLAIFALSVTFAPA
IGPTIGGYLTENYGWQTIFFINAIPSAVMAAALALTLEKQPMQLGLLKEGDWAGIVTMAIGLSALQTVLEEGNKEDWFAS
SFIVKLSILAFVFLAAFIWIELTVKKPLVKLSLLTQRNFGIGVAVNVLVGVALFGTVYILPQYLGQVQRYNAEQIGNVLA
WTGLPQLLLIPLVPMMMKRFDARYIGFLGISIFAISCFLNITLSADTAGDQFWIPNIVRAVGQALVLTPITAITTAGIAP
ADAAAASGLTNMLRNLGGAVGTATLGTVLTKREQFHSNIIGQSVTLGRDEVRDRLAQTTAYFMQHGVSDPAVAAHKAVAA
LGQIVRRQALILGFADTFAVIGVVLAIAAVALLLTQKPRAGAGAGAH

Sequences:

>Translated_527_residues
MSSIAATAGAIAQPRASTRDWIAVLAGMIGAFMAILNIQITNASLLDIEGGIGTGVDNGAWISTSYLIGEIVVIPLTAYF
SSVFSFRRYILVNSILFPLFSIACAFAHDLGTMILLRGLQGFAGGVLIPMAFTMVLTKLPKSQQPLGLAIFALSVTFAPA
IGPTIGGYLTENYGWQTIFFINAIPSAVMAAALALTLEKQPMQLGLLKEGDWAGIVTMAIGLSALQTVLEEGNKEDWFAS
SFIVKLSILAFVFLAAFIWIELTVKKPLVKLSLLTQRNFGIGVAVNVLVGVALFGTVYILPQYLGQVQRYNAEQIGNVLA
WTGLPQLLLIPLVPMMMKRFDARYIGFLGISIFAISCFLNITLSADTAGDQFWIPNIVRAVGQALVLTPITAITTAGIAP
ADAAAASGLTNMLRNLGGAVGTATLGTVLTKREQFHSNIIGQSVTLGRDEVRDRLAQTTAYFMQHGVSDPAVAAHKAVAA
LGQIVRRQALILGFADTFAVIGVVLAIAAVALLLTQKPRAGAGAGAH
>Mature_526_residues
SSIAATAGAIAQPRASTRDWIAVLAGMIGAFMAILNIQITNASLLDIEGGIGTGVDNGAWISTSYLIGEIVVIPLTAYFS
SVFSFRRYILVNSILFPLFSIACAFAHDLGTMILLRGLQGFAGGVLIPMAFTMVLTKLPKSQQPLGLAIFALSVTFAPAI
GPTIGGYLTENYGWQTIFFINAIPSAVMAAALALTLEKQPMQLGLLKEGDWAGIVTMAIGLSALQTVLEEGNKEDWFASS
FIVKLSILAFVFLAAFIWIELTVKKPLVKLSLLTQRNFGIGVAVNVLVGVALFGTVYILPQYLGQVQRYNAEQIGNVLAW
TGLPQLLLIPLVPMMMKRFDARYIGFLGISIFAISCFLNITLSADTAGDQFWIPNIVRAVGQALVLTPITAITTAGIAPA
DAAAASGLTNMLRNLGGAVGTATLGTVLTKREQFHSNIIGQSVTLGRDEVRDRLAQTTAYFMQHGVSDPAVAAHKAVAAL
GQIVRRQALILGFADTFAVIGVVLAIAAVALLLTQKPRAGAGAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=480, Percent_Identity=29.5833333333333, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1788710, Length=426, Percent_Identity=28.8732394366197, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=25.2927400468384, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790195, Length=435, Percent_Identity=24.1379310344828, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788392, Length=269, Percent_Identity=27.1375464684015, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55726; Mature: 55595

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIAATAGAIAQPRASTRDWIAVLAGMIGAFMAILNIQITNASLLDIEGGIGTGVDNGA
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCC
WISTSYLIGEIVVIPLTAYFSSVFSFRRYILVNSILFPLFSIACAFAHDLGTMILLRGLQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFAGGVLIPMAFTMVLTKLPKSQQPLGLAIFALSVTFAPAIGPTIGGYLTENYGWQTIFF
HHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEE
INAIPSAVMAAALALTLEKQPMQLGLLKEGDWAGIVTMAIGLSALQTVLEEGNKEDWFAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SFIVKLSILAFVFLAAFIWIELTVKKPLVKLSLLTQRNFGIGVAVNVLVGVALFGTVYIL
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PQYLGQVQRYNAEQIGNVLAWTGLPQLLLIPLVPMMMKRFDARYIGFLGISIFAISCFLN
HHHHHHHHHCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
ITLSADTAGDQFWIPNIVRAVGQALVLTPITAITTAGIAPADAAAASGLTNMLRNLGGAV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
GTATLGTVLTKREQFHSNIIGQSVTLGRDEVRDRLAQTTAYFMQHGVSDPAVAAHKAVAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGQIVRRQALILGFADTFAVIGVVLAIAAVALLLTQKPRAGAGAGAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SSIAATAGAIAQPRASTRDWIAVLAGMIGAFMAILNIQITNASLLDIEGGIGTGVDNGA
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]