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The map label for this gene is gsiB [H]

Identifier: 190894570

GI number: 190894570

Start: 231234

End: 232748

Strand: Reverse

Name: gsiB [H]

Synonym: RHECIAT_PC0000232

Alternate gene names: 190894570

Gene position: 232748-231234 (Counterclockwise)

Preceding gene: 190894571

Following gene: 190894562

Centisome position: 21.32

GC content: 59.34

Gene sequence:

>1515_bases
ATGACAAACAGGTGGAAATCAATCGGGCTCGCAGCCTTGCTCGCCGGCCTGACGCTCAGCGCAAGCTATGCCGACGCCGC
CGGTGTGCTCACCATCGGCCGCCGCGAGGATTCGACGACGTTCGATCCGATCAAAACAGCCCAGAACATCGACAACTGGG
TATTCTCAAACGTCTACGACGTGCTGATCCGCGTCGACAAGACAGGCACGAAGCTGGAGCCCGGTCTTGCCGAAAGCTGG
ACGGCCGCGGATGACGGCCTGACCTACACGTTCAAGATTCGCGACACTAAATTCTCGGACGGTTCGCCGCTGACGGCGGA
AGACGCCGCTTACAGCCTGCTGCGGATTCGCGACGACCCTGCTTCGCTCTGGAGCGATTCCTACAAGGTGATCGATACGG
CGCTGGCCACCGACGCGCATACGCTGACGATCAAGCTCAAGAACCCGTCCGCGCCGTTCCTGTCGACGCTGGCGCTGCCG
AACGCCTCCGTCATCTCCAAGAAGGGCATGGAATCGCTGGGGGCGGATGCCTACGGTGAAAAGCCGATCGCATCCGGCGC
CTTCGTGATCGAGGAATGGCGGCGCGGCGACCGGATCATCCTGAAGAAGAACCCGAATTTCTGGCAGGCCGACCGCGTCA
AGCTCGACGGCGTCGAATGGATCTCGGTGCCCGACGACAATACCCGCATGCTGAACGTCCAGGCCGGCGAGCTGGATGCA
GCGATCTTCGTGCCCTTCTCCCGGGTCGAGGAACTGAAGAAGGATCCTAACCTCAAAGTCGCTATCGACGCCTCGACGCG
TGAGGATCATTTGCTGATCAACCATGCGCATGGCGCGCTCGGCAAGAAGGAAGTCCGCCAGGCGCTGGATCTTGCGATCG
ACAAAAAGGCGATCGTCGATACCGTCACCTTCGGCCAGGGCACCGTCGCCAATTCCTACATTCCGAAGGGTGCGCTCTAT
TATTACGCCGACAATCTGCAGCGGCCTTATGATCCCGCCAAGGCCAAGGAGATGCTGGCGGCCGCCGGCGCATCCGACCT
GACGCTCAACTATCTGGTGCGCGCCGGCGACGAAGTCGACGAGCAGACGGCCGTCCTGGTTCAGCAGCAGCTGCAGAAAG
CCGGCATTACCGCCAACCTACAGAAGGTCGATCCGAGCCAGGAATGGGACATGACGGTTGCCGGCGACTACGACATCTCG
GTCAACTATTGGACGAATGACATTCTCGATCCGGACCAGAAGACCACCTTCGTTCTCGGCCACGATTCCAACAACAACTA
CCTGACGAACTACAAGAACGAGGCGGTGAAGGAGCTGGTCGCCAAGGCTCGCCTCGAGCTCGACCCGAAGAAGCGCGAAG
CGATGTATGTTGATCTGCAGAAGATGGCTAAGGACGACGTCAACTGGATCGATCTTTACTACAGCCCCTATATCAACGTT
TCGCGCAAGAATATCGAGAACTTCTACCAGAACCCGCTCGGCCGCTTCTTCCTGGAGGATACGGTCAAGAATTGA

Upstream 100 bases:

>100_bases
GACAAGCCGCTGCTGCAGGGCGCCGGCGGCAAGGAGGATCTGCAATCGATACCGATCCAGCCCATCAAACGCATGCCATA
ACAACGAAGGGAACGAACTC

Downstream 100 bases:

>100_bases
GTTCCGGCAGCATCAATGCTCCGTCGCCGTAAAGGCGGCGGAGCTTCATTTGTTGCTGCGGCAAGTGGCTGGGGCAGCTT
GGTTACTCGGCGGCCGCGAG

Product: putative dipeptide ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MTNRWKSIGLAALLAGLTLSASYADAAGVLTIGRREDSTTFDPIKTAQNIDNWVFSNVYDVLIRVDKTGTKLEPGLAESW
TAADDGLTYTFKIRDTKFSDGSPLTAEDAAYSLLRIRDDPASLWSDSYKVIDTALATDAHTLTIKLKNPSAPFLSTLALP
NASVISKKGMESLGADAYGEKPIASGAFVIEEWRRGDRIILKKNPNFWQADRVKLDGVEWISVPDDNTRMLNVQAGELDA
AIFVPFSRVEELKKDPNLKVAIDASTREDHLLINHAHGALGKKEVRQALDLAIDKKAIVDTVTFGQGTVANSYIPKGALY
YYADNLQRPYDPAKAKEMLAAAGASDLTLNYLVRAGDEVDEQTAVLVQQQLQKAGITANLQKVDPSQEWDMTVAGDYDIS
VNYWTNDILDPDQKTTFVLGHDSNNNYLTNYKNEAVKELVAKARLELDPKKREAMYVDLQKMAKDDVNWIDLYYSPYINV
SRKNIENFYQNPLGRFFLEDTVKN

Sequences:

>Translated_504_residues
MTNRWKSIGLAALLAGLTLSASYADAAGVLTIGRREDSTTFDPIKTAQNIDNWVFSNVYDVLIRVDKTGTKLEPGLAESW
TAADDGLTYTFKIRDTKFSDGSPLTAEDAAYSLLRIRDDPASLWSDSYKVIDTALATDAHTLTIKLKNPSAPFLSTLALP
NASVISKKGMESLGADAYGEKPIASGAFVIEEWRRGDRIILKKNPNFWQADRVKLDGVEWISVPDDNTRMLNVQAGELDA
AIFVPFSRVEELKKDPNLKVAIDASTREDHLLINHAHGALGKKEVRQALDLAIDKKAIVDTVTFGQGTVANSYIPKGALY
YYADNLQRPYDPAKAKEMLAAAGASDLTLNYLVRAGDEVDEQTAVLVQQQLQKAGITANLQKVDPSQEWDMTVAGDYDIS
VNYWTNDILDPDQKTTFVLGHDSNNNYLTNYKNEAVKELVAKARLELDPKKREAMYVDLQKMAKDDVNWIDLYYSPYINV
SRKNIENFYQNPLGRFFLEDTVKN
>Mature_503_residues
TNRWKSIGLAALLAGLTLSASYADAAGVLTIGRREDSTTFDPIKTAQNIDNWVFSNVYDVLIRVDKTGTKLEPGLAESWT
AADDGLTYTFKIRDTKFSDGSPLTAEDAAYSLLRIRDDPASLWSDSYKVIDTALATDAHTLTIKLKNPSAPFLSTLALPN
ASVISKKGMESLGADAYGEKPIASGAFVIEEWRRGDRIILKKNPNFWQADRVKLDGVEWISVPDDNTRMLNVQAGELDAA
IFVPFSRVEELKKDPNLKVAIDASTREDHLLINHAHGALGKKEVRQALDLAIDKKAIVDTVTFGQGTVANSYIPKGALYY
YADNLQRPYDPAKAKEMLAAAGASDLTLNYLVRAGDEVDEQTAVLVQQQLQKAGITANLQKVDPSQEWDMTVAGDYDISV
NYWTNDILDPDQKTTFVLGHDSNNNYLTNYKNEAVKELVAKARLELDPKKREAMYVDLQKMAKDDVNWIDLYYSPYINVS
RKNIENFYQNPLGRFFLEDTVKN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=507, Percent_Identity=29.3885601577909, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787052, Length=503, Percent_Identity=29.4234592445328, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789966, Length=475, Percent_Identity=25.4736842105263, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787551, Length=488, Percent_Identity=25.4098360655738, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787495, Length=514, Percent_Identity=26.4591439688716, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789397, Length=501, Percent_Identity=26.7465069860279, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789887, Length=510, Percent_Identity=23.5294117647059, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87081878, Length=504, Percent_Identity=24.6031746031746, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56083; Mature: 55951

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRWKSIGLAALLAGLTLSASYADAAGVLTIGRREDSTTFDPIKTAQNIDNWVFSNVYD
CCCCHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEE
VLIRVDKTGTKLEPGLAESWTAADDGLTYTFKIRDTKFSDGSPLTAEDAAYSLLRIRDDP
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHEEEEEEECCH
ASLWSDSYKVIDTALATDAHTLTIKLKNPSAPFLSTLALPNASVISKKGMESLGADAYGE
HHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCHHHHHHCHHHHCCCCCCC
KPIASGAFVIEEWRRGDRIILKKNPNFWQADRVKLDGVEWISVPDDNTRMLNVQAGELDA
CCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEECCCCCE
AIFVPFSRVEELKKDPNLKVAIDASTREDHLLINHAHGALGKKEVRQALDLAIDKKAIVD
EEECCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHEE
TVTFGQGTVANSYIPKGALYYYADNLQRPYDPAKAKEMLAAAGASDLTLNYLVRAGDEVD
EEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHCCCCCC
EQTAVLVQQQLQKAGITANLQKVDPSQEWDMTVAGDYDISVNYWTNDILDPDQKTTFVLG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEEEEECCCCCCCCCCCEEEEEE
HDSNNNYLTNYKNEAVKELVAKARLELDPKKREAMYVDLQKMAKDDVNWIDLYYSPYINV
ECCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHEEEHHHHCCCCCCEEEEEECCCEEC
SRKNIENFYQNPLGRFFLEDTVKN
CHHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure 
TNRWKSIGLAALLAGLTLSASYADAAGVLTIGRREDSTTFDPIKTAQNIDNWVFSNVYD
CCCHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEE
VLIRVDKTGTKLEPGLAESWTAADDGLTYTFKIRDTKFSDGSPLTAEDAAYSLLRIRDDP
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHEEEEEEECCH
ASLWSDSYKVIDTALATDAHTLTIKLKNPSAPFLSTLALPNASVISKKGMESLGADAYGE
HHHHCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCHHHHHHCHHHHCCCCCCC
KPIASGAFVIEEWRRGDRIILKKNPNFWQADRVKLDGVEWISVPDDNTRMLNVQAGELDA
CCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEECCCCCE
AIFVPFSRVEELKKDPNLKVAIDASTREDHLLINHAHGALGKKEVRQALDLAIDKKAIVD
EEECCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHEE
TVTFGQGTVANSYIPKGALYYYADNLQRPYDPAKAKEMLAAAGASDLTLNYLVRAGDEVD
EEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEHHHHCCCCCC
EQTAVLVQQQLQKAGITANLQKVDPSQEWDMTVAGDYDISVNYWTNDILDPDQKTTFVLG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEEEEECCCCCCCCCCCEEEEEE
HDSNNNYLTNYKNEAVKELVAKARLELDPKKREAMYVDLQKMAKDDVNWIDLYYSPYINV
ECCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHEEEHHHHCCCCCCEEEEEECCCEEC
SRKNIENFYQNPLGRFFLEDTVKN
CHHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA