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The map label for this gene is nylA [H]

Identifier: 190894551

GI number: 190894551

Start: 214642

End: 216054

Strand: Reverse

Name: nylA [H]

Synonym: RHECIAT_PC0000213

Alternate gene names: 190894551

Gene position: 216054-214642 (Counterclockwise)

Preceding gene: 190894552

Following gene: 190894550

Centisome position: 19.79

GC content: 67.94

Gene sequence:

>1413_bases
ATGCAGGGCGATGCGACGGAACTGGCGGCAGCGATTAGGCATGGCAGTCTGAGCGCTGCAGAAGCGATGCAGACCTCCCT
CGAGGCTGCCGCGCGGCATGCGCCGCTCGGCGCGATCGCCTATCTCGACGGTGCCATGGGTCTCGCCGCGGCGCAGGCCT
GCGACCGGGAGCGGCAGAGCGCGCCCGAGCATTTCTCGGCGCGGCCCTTTGCCGGCGTGCCGACCTTGGCGAAGGATCTT
GGCGGTCCCTTCCGCGGACTGCCGGTAACGGCCGGTTCCCGCCTCTTCGAGAGAAAGGGCGGCGACGCCGACTCCGATCT
CGCTGCGCGGTTCCGCGAGGCCGGCTTCTGTCTGTTCGGCCTGACGACGAGCCCGGAATTCGGCCTTTCGCTCGCCAGCG
AACCGGCGATCGGACCTGTCTGTCGCAACCCGCTCGATCCCACGCGCACCGCCGGCGGCTCGTCCGGCGGTGCGGCGGCA
GCGGTTGCTGCCGGGATCGTCGCGATTGCCCATGCCACCGATGCCGGCGGCTCGATCCGCGTGCCCGCCGCCTGCTGCGG
TCTCGTCGGAATGAAGCCGACCCGCGGCGCCATCCCCGGCGGGCCGTCTTTCGGCAACCACCTTGCCGGCATTGCGAGCG
AACTGGCGGTCTGCCGCTCGGTGCGGGATACTGCGCTGATTTTCGACCGGCTGAGCGGCAATGCACGCGGACCCGTTCCC
GATCCCTCTCCCGCCGATGTCGACACCGGCCGTTTGCGGATCGGCCTGTTGACGGATACCGGACCCGCCCACCCGACCGA
GGGTGATCGACTTGCGGCCGTCGCGGACGCCGCGCGTGTGCTCGAGAAGGACGGACACGCCATCGTTCCATTGGCCTGGG
CCGACTTCGAATGGAGCGCCGCCGCCAGCGGCCGCGCCTTTGCGGATATCGTTTCGGTCAATCTCGCCGCCCTCGTCGAG
GCGGCAGCGCTTGAGGAAAGCAGGGCCGAGCCCCTCACCCGGGCTTTCGCTAGACGCGGACGAACGCTTTCGGCCACCGC
GCTCTGGAAAACACTGAACGATGCGGTGCTGGTGAGCCATGCGGTCTGGGGGCTATTCGATACGGTCGATTGCATCCTGA
TGCCGATGCTCGCCTCCGCGCCTCTTGCGATCGGCTCCTTTCCGTCCGATCATGCCGACACCGATCTCCATCTGGAGCGA
ATGACCGCGTTTGCGCCGCTTGCCTGCCTTGCCAATGTCTCGGGTTTTCCTGCTTTAACGCTGCCTTTTGGACAGGATCA
GCACGCCATGCCGCTGCCGGTGCAGCTCATGATGCCGATGGGTCACGAGCCCCGGCTTCTGTCGCTTGCGGCACGCCTGG
AGGCCGAGGCGCGATGGCAGCACCGTTTTCCAGTGGCGGGGTTGCCGTCATGA

Upstream 100 bases:

>100_bases
CTGCCGAGGCGGAGGCCGCGATGGAAGCCAAGCGGGCGGAGATCAAGGCCGGCACCTTCGAAGTTCCGAAGATGATGGAA
GAGCCGAAGTAGTTCGGCTG

Downstream 100 bases:

>100_bases
CCGGGCCTGTTCTGGAAATTGTCGGCGTCAGCAAACGCTTCGGCGCCAATCTCGCCAATGACGATATTTCCATGACGCTG
GCCAGGGGCGAGGTCGTCGC

Product: putative amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQGDATELAAAIRHGSLSAAEAMQTSLEAAARHAPLGAIAYLDGAMGLAAAQACDRERQSAPEHFSARPFAGVPTLAKDL
GGPFRGLPVTAGSRLFERKGGDADSDLAARFREAGFCLFGLTTSPEFGLSLASEPAIGPVCRNPLDPTRTAGGSSGGAAA
AVAAGIVAIAHATDAGGSIRVPAACCGLVGMKPTRGAIPGGPSFGNHLAGIASELAVCRSVRDTALIFDRLSGNARGPVP
DPSPADVDTGRLRIGLLTDTGPAHPTEGDRLAAVADAARVLEKDGHAIVPLAWADFEWSAAASGRAFADIVSVNLAALVE
AAALEESRAEPLTRAFARRGRTLSATALWKTLNDAVLVSHAVWGLFDTVDCILMPMLASAPLAIGSFPSDHADTDLHLER
MTAFAPLACLANVSGFPALTLPFGQDQHAMPLPVQLMMPMGHEPRLLSLAARLEAEARWQHRFPVAGLPS

Sequences:

>Translated_470_residues
MQGDATELAAAIRHGSLSAAEAMQTSLEAAARHAPLGAIAYLDGAMGLAAAQACDRERQSAPEHFSARPFAGVPTLAKDL
GGPFRGLPVTAGSRLFERKGGDADSDLAARFREAGFCLFGLTTSPEFGLSLASEPAIGPVCRNPLDPTRTAGGSSGGAAA
AVAAGIVAIAHATDAGGSIRVPAACCGLVGMKPTRGAIPGGPSFGNHLAGIASELAVCRSVRDTALIFDRLSGNARGPVP
DPSPADVDTGRLRIGLLTDTGPAHPTEGDRLAAVADAARVLEKDGHAIVPLAWADFEWSAAASGRAFADIVSVNLAALVE
AAALEESRAEPLTRAFARRGRTLSATALWKTLNDAVLVSHAVWGLFDTVDCILMPMLASAPLAIGSFPSDHADTDLHLER
MTAFAPLACLANVSGFPALTLPFGQDQHAMPLPVQLMMPMGHEPRLLSLAARLEAEARWQHRFPVAGLPS
>Mature_470_residues
MQGDATELAAAIRHGSLSAAEAMQTSLEAAARHAPLGAIAYLDGAMGLAAAQACDRERQSAPEHFSARPFAGVPTLAKDL
GGPFRGLPVTAGSRLFERKGGDADSDLAARFREAGFCLFGLTTSPEFGLSLASEPAIGPVCRNPLDPTRTAGGSSGGAAA
AVAAGIVAIAHATDAGGSIRVPAACCGLVGMKPTRGAIPGGPSFGNHLAGIASELAVCRSVRDTALIFDRLSGNARGPVP
DPSPADVDTGRLRIGLLTDTGPAHPTEGDRLAAVADAARVLEKDGHAIVPLAWADFEWSAAASGRAFADIVSVNLAALVE
AAALEESRAEPLTRAFARRGRTLSATALWKTLNDAVLVSHAVWGLFDTVDCILMPMLASAPLAIGSFPSDHADTDLHLER
MTAFAPLACLANVSGFPALTLPFGQDQHAMPLPVQLMMPMGHEPRLLSLAARLEAEARWQHRFPVAGLPS

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=241, Percent_Identity=30.2904564315353, Blast_Score=80, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=437, Percent_Identity=25.629290617849, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=303, Percent_Identity=26.0726072607261, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=187, Percent_Identity=34.2245989304813, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=187, Percent_Identity=34.2245989304813, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32565337, Length=220, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=25.3275109170306, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=129, Percent_Identity=39.5348837209302, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=305, Percent_Identity=27.2131147540984, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=23.8289205702648, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=27.5510204081633, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550774, Length=208, Percent_Identity=27.8846153846154, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24652985, Length=207, Percent_Identity=32.3671497584541, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19922090, Length=207, Percent_Identity=32.3671497584541, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24652981, Length=207, Percent_Identity=32.3671497584541, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24652983, Length=207, Percent_Identity=32.3671497584541, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648435, Length=487, Percent_Identity=21.1498973305955, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648437, Length=487, Percent_Identity=21.1498973305955, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648113, Length=508, Percent_Identity=25.7874015748031, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161078093, Length=213, Percent_Identity=28.6384976525822, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 48433; Mature: 48433

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGDATELAAAIRHGSLSAAEAMQTSLEAAARHAPLGAIAYLDGAMGLAAAQACDRERQS
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
APEHFSARPFAGVPTLAKDLGGPFRGLPVTAGSRLFERKGGDADSDLAARFREAGFCLFG
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCEEEEE
LTTSPEFGLSLASEPAIGPVCRNPLDPTRTAGGSSGGAAAAVAAGIVAIAHATDAGGSIR
CCCCCCCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEE
VPAACCGLVGMKPTRGAIPGGPSFGNHLAGIASELAVCRSVRDTALIFDRLSGNARGPVP
CHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DPSPADVDTGRLRIGLLTDTGPAHPTEGDRLAAVADAARVLEKDGHAIVPLAWADFEWSA
CCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AASGRAFADIVSVNLAALVEAAALEESRAEPLTRAFARRGRTLSATALWKTLNDAVLVSH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
AVWGLFDTVDCILMPMLASAPLAIGSFPSDHADTDLHLERMTAFAPLACLANVSGFPALT
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
LPFGQDQHAMPLPVQLMMPMGHEPRLLSLAARLEAEARWQHRFPVAGLPS
ECCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQGDATELAAAIRHGSLSAAEAMQTSLEAAARHAPLGAIAYLDGAMGLAAAQACDRERQS
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
APEHFSARPFAGVPTLAKDLGGPFRGLPVTAGSRLFERKGGDADSDLAARFREAGFCLFG
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCEEEEE
LTTSPEFGLSLASEPAIGPVCRNPLDPTRTAGGSSGGAAAAVAAGIVAIAHATDAGGSIR
CCCCCCCCCEECCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEE
VPAACCGLVGMKPTRGAIPGGPSFGNHLAGIASELAVCRSVRDTALIFDRLSGNARGPVP
CHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DPSPADVDTGRLRIGLLTDTGPAHPTEGDRLAAVADAARVLEKDGHAIVPLAWADFEWSA
CCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AASGRAFADIVSVNLAALVEAAALEESRAEPLTRAFARRGRTLSATALWKTLNDAVLVSH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHH
AVWGLFDTVDCILMPMLASAPLAIGSFPSDHADTDLHLERMTAFAPLACLANVSGFPALT
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
LPFGQDQHAMPLPVQLMMPMGHEPRLLSLAARLEAEARWQHRFPVAGLPS
ECCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]