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The map label for this gene is rutB [H]

Identifier: 190894426

GI number: 190894426

Start: 83897

End: 84598

Strand: Reverse

Name: rutB [H]

Synonym: RHECIAT_PC0000088

Alternate gene names: 190894426

Gene position: 84598-83897 (Counterclockwise)

Preceding gene: 190894427

Following gene: 190894425

Centisome position: 7.75

GC content: 60.68

Gene sequence:

>702_bases
ATGGACGCCATGGTCGAAACCAAAGGACATTATATCGATGCGGACCCCTATGCCTGGCCGTATAACGGCGATCTGAGACC
GGAGAACACGGCCCTCATCATCATCGACATGCAGACGGATTTCTGCGGCAAGGGCGGCTATGTCGATCACATGGGTTATG
ACCTGTCGCTCGTCCAGGCGCCGATCGAGCCGATCAAACGCGTGCTGGCCGCCATGCGCGCCAAGGGCTATCACATCATC
CACACGCGCGAGGGCCATCGGCCCGACCTTGCCGACCTGCCGGCCAACAAACGCTGGCGCTCGCAGCGGATCGGCGCCGG
GATCGGCGATCCGGGTCCTTGCGGGCGTATCCTGACCCGCGGCGAACCCGGATGGGACATCATCCCCGAACTCTATCCGA
TCGAAGGCGAAACGATCATCGACAAGCCGGGCAAGGGTTCCTTCTGCGCCACCGACCTTGAATTGATCCTCAACCAGAAG
CGCATCGCAAACATCATCCTGACCGGCATCACCACCGATGTCTGCGTCTCCACCACGATGCGCGAGGCCAATGACCGCGG
TTTCGAATGCCTGCTGCTGGAAGATTGCTGCGGCGCGACCGATTACGGCAACCATCTTGCCGCCATCAAGATGGTGAAGA
TGCAGGGCGGCGTCTTCGGCTCTGTCTCCAATTCGCAAACGCTTGTGAGCCAGCTGCCATGA

Upstream 100 bases:

>100_bases
GGAGCAGCGCATGTCCGGCACCGGCGCATCGGACCGATCGGAACAGCCGCACCACCACCGCGACACCTTCAAACTCGCAG
GCGATTAGGAGACGAAGACG

Downstream 100 bases:

>100_bases
GCACCGTCCGCGACACGCCTCTCCCGCACGCCGGTAAAGCCGTCGGCATCGATACGCTCGACATGACGATGCGCTTCGGC
AGCTTCACGGCCCTCGACCG

Product: putative isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDAMVETKGHYIDADPYAWPYNGDLRPENTALIIIDMQTDFCGKGGYVDHMGYDLSLVQAPIEPIKRVLAAMRAKGYHII
HTREGHRPDLADLPANKRWRSQRIGAGIGDPGPCGRILTRGEPGWDIIPELYPIEGETIIDKPGKGSFCATDLELILNQK
RIANIILTGITTDVCVSTTMREANDRGFECLLLEDCCGATDYGNHLAAIKMVKMQGGVFGSVSNSQTLVSQLP

Sequences:

>Translated_233_residues
MDAMVETKGHYIDADPYAWPYNGDLRPENTALIIIDMQTDFCGKGGYVDHMGYDLSLVQAPIEPIKRVLAAMRAKGYHII
HTREGHRPDLADLPANKRWRSQRIGAGIGDPGPCGRILTRGEPGWDIIPELYPIEGETIIDKPGKGSFCATDLELILNQK
RIANIILTGITTDVCVSTTMREANDRGFECLLLEDCCGATDYGNHLAAIKMVKMQGGVFGSVSNSQTLVSQLP
>Mature_233_residues
MDAMVETKGHYIDADPYAWPYNGDLRPENTALIIIDMQTDFCGKGGYVDHMGYDLSLVQAPIEPIKRVLAAMRAKGYHII
HTREGHRPDLADLPANKRWRSQRIGAGIGDPGPCGRILTRGEPGWDIIPELYPIEGETIIDKPGKGSFCATDLELILNQK
RIANIILTGITTDVCVSTTMREANDRGFECLLLEDCCGATDYGNHLAAIKMVKMQGGVFGSVSNSQTLVSQLP

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=209, Percent_Identity=29.1866028708134, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25546; Mature: 25546

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAMVETKGHYIDADPYAWPYNGDLRPENTALIIIDMQTDFCGKGGYVDHMGYDLSLVQA
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
PIEPIKRVLAAMRAKGYHIIHTREGHRPDLADLPANKRWRSQRIGAGIGDPGPCGRILTR
HHHHHHHHHHHHHHCCEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCCCCCCCCCCEEEC
GEPGWDIIPELYPIEGETIIDKPGKGSFCATDLELILNQKRIANIILTGITTDVCVSTTM
CCCCCCCCCCEECCCCCEEEECCCCCCEEHHHHHHHHCHHHHHEEEEECCCHHHHHHHHH
REANDRGFECLLLEDCCGATDYGNHLAAIKMVKMQGGVFGSVSNSQTLVSQLP
HHCCCCCEEEEEEHHHCCCCCCCCCEEEEEEEEECCCEEECCCCCHHHHHHCC
>Mature Secondary Structure
MDAMVETKGHYIDADPYAWPYNGDLRPENTALIIIDMQTDFCGKGGYVDHMGYDLSLVQA
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
PIEPIKRVLAAMRAKGYHIIHTREGHRPDLADLPANKRWRSQRIGAGIGDPGPCGRILTR
HHHHHHHHHHHHHHCCEEEEEECCCCCCCHHCCCCCCCHHHHHCCCCCCCCCCCCCEEEC
GEPGWDIIPELYPIEGETIIDKPGKGSFCATDLELILNQKRIANIILTGITTDVCVSTTM
CCCCCCCCCCEECCCCCEEEECCCCCCEEHHHHHHHHCHHHHHEEEEECCCHHHHHHHHH
REANDRGFECLLLEDCCGATDYGNHLAAIKMVKMQGGVFGSVSNSQTLVSQLP
HHCCCCCEEEEEEHHHCCCCCCCCCEEEEEEEEECCCEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA