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The map label for this gene is recF [H]

Identifier: 190571298

GI number: 190571298

Start: 967664

End: 968743

Strand: Direct

Name: recF [H]

Synonym: WPa_0901

Alternate gene names: 190571298

Gene position: 967664-968743 (Clockwise)

Preceding gene: 190571297

Following gene: 190571302

Centisome position: 65.27

GC content: 32.13

Gene sequence:

>1080_bases
ATGACTACCCATTGCTATATAAAAAAACTGAAATTATATAACTTTCGTAGTCATTCAAACTTTGAACTGGATTTAGATGA
TAGACCAGTTGTAGTAACTGGCAAAAATGGTATTGGAAAAACTAATATACTTGAAGCAATCTCATTGCTTGCTAAAAGCA
ACGGAATGAAAAAAGCAAAAATCAATGAGATGCAAAATAGGCGCAGTAATGAAAGTTGGGTAGTGTATTATGATTTTTTC
AATGGAGCAGAGCTTAACTCAATTGGTATAGGAAAGAATCTTAATAAGAAATTAATACAAATTGATGGGAAAACGCAGTC
AAGCTACTCATCTTTGTATAGAATATCTAATGTAATATGGCTAATTCCACAAATGGACTATATTCTTCTTAATTCTCCAA
GTGACAGGTTAAAATTTTTAGACCGTATAGTTTCACTATTTGAAGAAAATTACACCTATTGCTACATGAAATATAGAAAA
GCTAAGCATGAGCGAAGTAAATTGCTTAGAGAGAATATTTTGGACGAAAGTTGGCTCTCTAGTCTTGAAAACGTCATGGC
TACCAATGCAATTGATATTTTACGTATGCGATCATCTGTTTTAAAGATATTACAAGACACAATTGATAACCATTCTTGTG
AGTTTTTTCCAAAGGCAAGTTTGAAATTTAGCAGTCAGCTAACTTTGAACGATACTGCAGAATATTTTCAGAATCGTCTA
AAGGAAAACAGAGAAAAAGACTCATTAACTGGTAGAGTAACCTTTTCTGTACATAATGATAATTTTTGGGTTTTTTGCCA
AAAAGGGGACATGCCAATAAATCTTTGTTCTACTGGAGAGCAAAAGCTATTACTGCTTTCTATCATCTTATCTAGTGTAA
AAGCAAGGTGTATTCATTACAATAAAGCACCTCTTCTTCTGCTTGACGATATAATGTCGCATCTGGATAAAGATTACAGA
AAGGTGTTAATGGAGGAGGTGTTAAGCATTCAATGTCAAACTTGGATAACCGATGTAAATCAAGATAATTTCAATAACTA
TCTTTGCTCTTTCAAGTTTTTTGAATTATCAAGTAAATAA

Upstream 100 bases:

>100_bases
TAGATAAAAAAGGTAAAGTAGTGAAGATCTGGAAAAACGTAAAAGTTAGTGGACATGTTGATGAGGTTCTAGAAGAAGTA
AGGAGAATATAAATTAAAGA

Downstream 100 bases:

>100_bases
ATTTCAAGCGTGCTGTCATACATCAAGCTCATATTCGGTATGTAAATCTCTAATTAAAGCCTCAGCGTGTTTTTTTTGAA
CAACTATACTGATCTTGATT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTTHCYIKKLKLYNFRSHSNFELDLDDRPVVVTGKNGIGKTNILEAISLLAKSNGMKKAKINEMQNRRSNESWVVYYDFF
NGAELNSIGIGKNLNKKLIQIDGKTQSSYSSLYRISNVIWLIPQMDYILLNSPSDRLKFLDRIVSLFEENYTYCYMKYRK
AKHERSKLLRENILDESWLSSLENVMATNAIDILRMRSSVLKILQDTIDNHSCEFFPKASLKFSSQLTLNDTAEYFQNRL
KENREKDSLTGRVTFSVHNDNFWVFCQKGDMPINLCSTGEQKLLLLSIILSSVKARCIHYNKAPLLLLDDIMSHLDKDYR
KVLMEEVLSIQCQTWITDVNQDNFNNYLCSFKFFELSSK

Sequences:

>Translated_359_residues
MTTHCYIKKLKLYNFRSHSNFELDLDDRPVVVTGKNGIGKTNILEAISLLAKSNGMKKAKINEMQNRRSNESWVVYYDFF
NGAELNSIGIGKNLNKKLIQIDGKTQSSYSSLYRISNVIWLIPQMDYILLNSPSDRLKFLDRIVSLFEENYTYCYMKYRK
AKHERSKLLRENILDESWLSSLENVMATNAIDILRMRSSVLKILQDTIDNHSCEFFPKASLKFSSQLTLNDTAEYFQNRL
KENREKDSLTGRVTFSVHNDNFWVFCQKGDMPINLCSTGEQKLLLLSIILSSVKARCIHYNKAPLLLLDDIMSHLDKDYR
KVLMEEVLSIQCQTWITDVNQDNFNNYLCSFKFFELSSK
>Mature_358_residues
TTHCYIKKLKLYNFRSHSNFELDLDDRPVVVTGKNGIGKTNILEAISLLAKSNGMKKAKINEMQNRRSNESWVVYYDFFN
GAELNSIGIGKNLNKKLIQIDGKTQSSYSSLYRISNVIWLIPQMDYILLNSPSDRLKFLDRIVSLFEENYTYCYMKYRKA
KHERSKLLRENILDESWLSSLENVMATNAIDILRMRSSVLKILQDTIDNHSCEFFPKASLKFSSQLTLNDTAEYFQNRLK
ENREKDSLTGRVTFSVHNDNFWVFCQKGDMPINLCSTGEQKLLLLSIILSSVKARCIHYNKAPLLLLDDIMSHLDKDYRK
VLMEEVLSIQCQTWITDVNQDNFNNYLCSFKFFELSSK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=344, Percent_Identity=23.8372093023256, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41835; Mature: 41703

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHCYIKKLKLYNFRSHSNFELDLDDRPVVVTGKNGIGKTNILEAISLLAKSNGMKKAK
CCCCCEEEEEEEEEECCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHH
INEMQNRRSNESWVVYYDFFNGAELNSIGIGKNLNKKLIQIDGKTQSSYSSLYRISNVIW
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEE
LIPQMDYILLNSPSDRLKFLDRIVSLFEENYTYCYMKYRKAKHERSKLLRENILDESWLS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHH
SLENVMATNAIDILRMRSSVLKILQDTIDNHSCEFFPKASLKFSSQLTLNDTAEYFQNRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEEECHHHHHHHHHH
KENREKDSLTGRVTFSVHNDNFWVFCQKGDMPINLCSTGEQKLLLLSIILSSVKARCIHY
HHCCCCCCCEEEEEEEEECCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEC
NKAPLLLLDDIMSHLDKDYRKVLMEEVLSIQCQTWITDVNQDNFNNYLCSFKFFELSSK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure 
TTHCYIKKLKLYNFRSHSNFELDLDDRPVVVTGKNGIGKTNILEAISLLAKSNGMKKAK
CCCCEEEEEEEEEECCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHH
INEMQNRRSNESWVVYYDFFNGAELNSIGIGKNLNKKLIQIDGKTQSSYSSLYRISNVIW
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEE
LIPQMDYILLNSPSDRLKFLDRIVSLFEENYTYCYMKYRKAKHERSKLLRENILDESWLS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHH
SLENVMATNAIDILRMRSSVLKILQDTIDNHSCEFFPKASLKFSSQLTLNDTAEYFQNRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEEECHHHHHHHHHH
KENREKDSLTGRVTFSVHNDNFWVFCQKGDMPINLCSTGEQKLLLLSIILSSVKARCIHY
HHCCCCCCCEEEEEEEEECCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEC
NKAPLLLLDDIMSHLDKDYRKVLMEEVLSIQCQTWITDVNQDNFNNYLCSFKFFELSSK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]