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The map label for this gene is gshB [H]

Identifier: 190571296

GI number: 190571296

Start: 966198

End: 967124

Strand: Reverse

Name: gshB [H]

Synonym: WPa_0899

Alternate gene names: 190571296

Gene position: 967124-966198 (Counterclockwise)

Preceding gene: 190571299

Following gene: 190571294

Centisome position: 65.24

GC content: 32.47

Gene sequence:

>927_bases
ATGAAAGTAGCTTTTCAAATGGATGAAAATATAAATTTTGAAACTGATACTACATTTGCATTAATAAAAGAAGCGCAGAG
GAGAAAGCACGAAATTTTTGTCTATGTTCCTAATAATCTAGCACTCAAGTTAAATCAGTCAATTGCTCTTGCTCAGAAAG
TCAGCGTTGATGATTTTGGTTTTACTTCTAAAGAAGATGTAACTATAGATTTGAATGAAATGGATATCATACTTATCAGG
CAGGACCCACCTTTTGATATGCGGTACATTACAACAACCTATATCTTAGAAAAGACTACTGCGTTAGTAATAAATAATCC
AACAGAAATAAGAAACTGTCCTGAAAAATTAATTACTTCACTATTTCTAGAGCTAATTCCTCCAACTTTGATCACTGAAA
ATATATCGATGATTAGAAATTTTTGTCACGACTATCAAGATATCATCTTGAAACCACTATATAGCTATGGAGGAAATGAC
GTAATAAGAATACAAGACCAAAATAGCATTCAAGTGGTAGTCGATCTCATGATTGCAAAATATGAATGTCCTGTAATTGC
ACAAGCATTTTGTAAAAAGATAAATACAGATAAAAGAATACTGCTACTGGATGGAAAGCCAATAGGAGTAATGAAGCGAG
TTCCAAAGTCTAGCGGAGAAATCAGAACAAATATGAGGCTTGGAGCAAGTTTTGAATCTATTGAAATGAATGATAGAGAC
ACTGAAATATGTAATAAAATCGGTCCTGAGCTAAAGAAAAGAGGGCTAATATTTGTTGGTATTGATATTATAGATGACTT
CCTTCTTGAAATTAACACAACTTCGCCTACGGGAGTAGTTTATATCAATAAGTTGTATAGTAAATCACTAGAAAAAGAAC
TATGGGATGCATTTGAAGAAAAAGCAATTCGTCAACTAAGTTCTTGA

Upstream 100 bases:

>100_bases
TAAAATCAGGTGCATTATTTCCTACTGTTAATTCCATACTTTGCTCCATTATTAACACTTTTATGGTACTATAGTATATT
GTAATAGGAAGGGAGGATAA

Downstream 100 bases:

>100_bases
GCTATTGCCTTAATAAATTCAGTAACTCCATTCCTTATTCTTGTTTTCTTAGTATTATTAGCCATATCACGCAATTTTTG
ACAATTTTGCAATAAATCAA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKVAFQMDENINFETDTTFALIKEAQRRKHEIFVYVPNNLALKLNQSIALAQKVSVDDFGFTSKEDVTIDLNEMDIILIR
QDPPFDMRYITTTYILEKTTALVINNPTEIRNCPEKLITSLFLELIPPTLITENISMIRNFCHDYQDIILKPLYSYGGND
VIRIQDQNSIQVVVDLMIAKYECPVIAQAFCKKINTDKRILLLDGKPIGVMKRVPKSSGEIRTNMRLGASFESIEMNDRD
TEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYSKSLEKELWDAFEEKAIRQLSS

Sequences:

>Translated_308_residues
MKVAFQMDENINFETDTTFALIKEAQRRKHEIFVYVPNNLALKLNQSIALAQKVSVDDFGFTSKEDVTIDLNEMDIILIR
QDPPFDMRYITTTYILEKTTALVINNPTEIRNCPEKLITSLFLELIPPTLITENISMIRNFCHDYQDIILKPLYSYGGND
VIRIQDQNSIQVVVDLMIAKYECPVIAQAFCKKINTDKRILLLDGKPIGVMKRVPKSSGEIRTNMRLGASFESIEMNDRD
TEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYSKSLEKELWDAFEEKAIRQLSS
>Mature_308_residues
MKVAFQMDENINFETDTTFALIKEAQRRKHEIFVYVPNNLALKLNQSIALAQKVSVDDFGFTSKEDVTIDLNEMDIILIR
QDPPFDMRYITTTYILEKTTALVINNPTEIRNCPEKLITSLFLELIPPTLITENISMIRNFCHDYQDIILKPLYSYGGND
VIRIQDQNSIQVVVDLMIAKYECPVIAQAFCKKINTDKRILLLDGKPIGVMKRVPKSSGEIRTNMRLGASFESIEMNDRD
TEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYSKSLEKELWDAFEEKAIRQLSS

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=318, Percent_Identity=36.4779874213836, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35285; Mature: 35285

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAFQMDENINFETDTTFALIKEAQRRKHEIFVYVPNNLALKLNQSIALAQKVSVDDFG
CEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEHHHCCCCCCCC
FTSKEDVTIDLNEMDIILIRQDPPFDMRYITTTYILEKTTALVINNPTEIRNCPEKLITS
CCCCCCEEEEECCEEEEEEECCCCCCEEEEHHEEEEECCEEEEECCCHHHHCCHHHHHHH
LFLELIPPTLITENISMIRNFCHDYQDIILKPLYSYGGNDVIRIQDQNSIQVVVDLMIAK
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
YECPVIAQAFCKKINTDKRILLLDGKPIGVMKRVPKSSGEIRTNMRLGASFESIEMNDRD
CCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCCEEECCCCH
TEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYSKSLEKELWDAFEE
HHHHHHCCHHHHHCCEEEEEHHHHHCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
KAIRQLSS
HHHHHHCC
>Mature Secondary Structure
MKVAFQMDENINFETDTTFALIKEAQRRKHEIFVYVPNNLALKLNQSIALAQKVSVDDFG
CEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEHHHCCCCCCCC
FTSKEDVTIDLNEMDIILIRQDPPFDMRYITTTYILEKTTALVINNPTEIRNCPEKLITS
CCCCCCEEEEECCEEEEEEECCCCCCEEEEHHEEEEECCEEEEECCCHHHHCCHHHHHHH
LFLELIPPTLITENISMIRNFCHDYQDIILKPLYSYGGNDVIRIQDQNSIQVVVDLMIAK
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
YECPVIAQAFCKKINTDKRILLLDGKPIGVMKRVPKSSGEIRTNMRLGASFESIEMNDRD
CCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCCEEECCCCH
TEICNKIGPELKKRGLIFVGIDIIDDFLLEINTTSPTGVVYINKLYSKSLEKELWDAFEE
HHHHHHCCHHHHHCCEEEEEHHHHHCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
KAIRQLSS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1540152 [H]