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The map label for this gene is trmD

Identifier: 190571275

GI number: 190571275

Start: 945050

End: 945748

Strand: Direct

Name: trmD

Synonym: WPa_0876

Alternate gene names: 190571275

Gene position: 945050-945748 (Clockwise)

Preceding gene: 190571273

Following gene: 190571276

Centisome position: 63.75

GC content: 36.91

Gene sequence:

>699_bases
ATGACGTTTAGTGTTACAATATTAACCATATTTCCAGAAATGTTCCCCGGGTTTTTGAACTATTCTCTTGCCGGAAAAGC
GTTGGAAAAAAAAATATGGAATCTTGAAATAGTAAATATTCGTTCTTTTGCTAAAGATAAACATTCAACTGTGGATGATG
TTCCATATGGAGGTGGAGCAGGGATGGTTATGCGCCCAGATGTGATTGGTAATGCAGTTGATAATGTGCTTTCTGCTCAT
AAAAATACTAGATTTATTTATATGACTCCATCTGGTACAAAATTTAATCAGAGAATTGCGAAGGAGTTAACAGAAATTCC
TCATGTAACAATATTGTGTGGTCGATTTGAAGGTGTTGACCAAAGAGTAATTGATGTGTATACTCCTTATGAGTTAAGTA
TTGGAGATTATATACTTTCGGGAGGTGAGCCAGCTGCGATGGTGGTTCTTGATGCATGTATTAGGCTTCTTCCAGGTGTA
GTAAATAATTTCGATAGCGTTGCTGAAGAAAGTTTTAGTTATAGTGGTGGTATACTTGAATATCCTCAATATACTAGACC
TGAGCAGTGGAGTAGGTACAAAGTTCCTGAGGTTCTGTTGTCTGGCAATCACAGAAAAATAAGTGATTGGAGAAGGGAAC
AGTCATATGTTAGAACAAAGGAACGTCGACCTGAATTGTTGAATGGAGATGATAAATGA

Upstream 100 bases:

>100_bases
GCTTTACCTTCGTATATTGTTTTATTCAGTGACATGATTAATTTAGCTGTATACTATAAGATAATATCAAATTGCTGCTT
AGTTTACTACAAATTTGCTA

Downstream 100 bases:

>100_bases
CAAACTTACTTGAAAAATTTAACGAACAACAAATACAAAGGTTAGCCAAAGAGATGCCAGCTTTTTGTCCTGGCGATGAT
TTGAAGGTCACTTTTAAGGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTFSVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEIVNIRSFAKDKHSTVDDVPYGGGAGMVMRPDVIGNAVDNVLSAH
KNTRFIYMTPSGTKFNQRIAKELTEIPHVTILCGRFEGVDQRVIDVYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGV
VNNFDSVAEESFSYSGGILEYPQYTRPEQWSRYKVPEVLLSGNHRKISDWRREQSYVRTKERRPELLNGDDK

Sequences:

>Translated_232_residues
MTFSVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEIVNIRSFAKDKHSTVDDVPYGGGAGMVMRPDVIGNAVDNVLSAH
KNTRFIYMTPSGTKFNQRIAKELTEIPHVTILCGRFEGVDQRVIDVYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGV
VNNFDSVAEESFSYSGGILEYPQYTRPEQWSRYKVPEVLLSGNHRKISDWRREQSYVRTKERRPELLNGDDK
>Mature_231_residues
TFSVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEIVNIRSFAKDKHSTVDDVPYGGGAGMVMRPDVIGNAVDNVLSAHK
NTRFIYMTPSGTKFNQRIAKELTEIPHVTILCGRFEGVDQRVIDVYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGVV
NNFDSVAEESFSYSGGILEYPQYTRPEQWSRYKVPEVLLSGNHRKISDWRREQSYVRTKERRPELLNGDDK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=42.6666666666667, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_WOLPP (B3CM65)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975633.1
- EnsemblBacteria:   EBWOLT00000000974
- GeneID:   6384579
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0876
- GeneTree:   EBGT00050000031385
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26232; Mature: 26101

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEIVNIRSFAKDKHSTVDDVPYGGGA
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCC
GMVMRPDVIGNAVDNVLSAHKNTRFIYMTPSGTKFNQRIAKELTEIPHVTILCGRFEGVD
CCEECCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
QRVIDVYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGVVNNFDSVAEESFSYSGGILE
CCEEEEECCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
YPQYTRPEQWSRYKVPEVLLSGNHRKISDWRREQSYVRTKERRPELLNGDDK
CCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TFSVTILTIFPEMFPGFLNYSLAGKALEKKIWNLEIVNIRSFAKDKHSTVDDVPYGGGA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCC
GMVMRPDVIGNAVDNVLSAHKNTRFIYMTPSGTKFNQRIAKELTEIPHVTILCGRFEGVD
CCEECCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
QRVIDVYTPYELSIGDYILSGGEPAAMVVLDACIRLLPGVVNNFDSVAEESFSYSGGILE
CCEEEEECCCEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
YPQYTRPEQWSRYKVPEVLLSGNHRKISDWRREQSYVRTKERRPELLNGDDK
CCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA