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The map label for this gene is tyrS

Identifier: 190571260

GI number: 190571260

Start: 932368

End: 933630

Strand: Direct

Name: tyrS

Synonym: WPa_0861

Alternate gene names: 190571260

Gene position: 932368-933630 (Clockwise)

Preceding gene: 190571259

Following gene: 190571262

Centisome position: 62.89

GC content: 35.39

Gene sequence:

>1263_bases
ATGAAACATAAATCCGAGTTTTTAAACTTCATTCAAGAAAGAGGATACCTATATCAATGTACAAACATTGATAGGTTAGA
CCAGCTATTATCACAAGATAATTATATAATTGCATACATTGGGTTTGATTGTACGGCACCAAGTTTGCATATTGGCAGTC
TCATTCAAATCATGATGCTCCGTCACCTACAAAAATTCGGTTATAAGCCAATAGTTCTACTTGGAGGTGGCACAACAAAA
ATTGGTGACCCATCTGGTAAAGACAAAGCAAGAAGCGTCTTGCCGATAGAAGATATCAATCAAAATATACTTGGTATAAA
GAAAACCCTCGAGAAGATGATATCTTTCGATTATGGAAAAACTGGTGCAATCATAGTAAACAACGCAGATTGGTTAGATA
ACATAAAATATATAGACTTTTTGCGTGATATAGGGACACATTTTTCTGTAAATCGCATGTTAGGCTTTGATAGTGTGAAA
ATTAGGCTAGATAGGGAGCAAAATCTAAGCTTTCTCGAGTTTAATTACATGTTGTTACAAGCCTATGATTTTGTCGAGTT
GAATAAAAAGTATGGCTGTCGTTTCCAGATTGGAGGGTCAGACCAATGGGGAAATATAGTAAACGGAATTGAACTTAGCA
AAAAGTTAAATTTACCTGAGCTGTTTGGTCTTACCACACCTCTTTTATTAAACGCACAAGGAAAAAAGATGGGCAAAACT
GAAAGTGGGGCAGTATGGCTTGATGGCAGCATGCTAAATTCTTATGATTACTGGCAATATTTTCGCAATGTTGATGATCA
AGATGTTGGTCGTTTTTTGAGGCTTTTCACTGATTTACCAATTGATGAAATTAAAAAACTAGAGTCCTTGAAGGATCAAG
AAACAAACGAAGCAAAAAAGGTTTTGGCAACAGAAGTGACGAAAATATGTCACGGTTGCAAAGAAGCCGAACTTGCACGA
TCTGCTGCAATCTCTGCTTTTGAAAATGAAGACAGTTCATTGCTTTCTGGTTACACTATTACAAAAGAACAAATTGCAAA
TGGTATACCCTTGATAGACCTGCTGTATGACACCGGTTTTGAACCTTCAAAAGGTGCAGCAAAGCGTTTAATACAAGGCA
ATGGATGCAAAGTTAATGATAATACTATAAACGATGTTAACTATACAATAAACTCTGAAAGCTTTAAAGGTCAGCCATTT
ATAAAATTATCTGCAGGAAAGAAACGACATATTAAAATTTTAGTGAGTGAAGTAAGAAAGTAA

Upstream 100 bases:

>100_bases
CAACATTTTTGATGAAATTTCATAATAAACTGGATTCCAGCGTCAAGCACTGGAATGACACCATCGTTAACAACACCGTT
TACACACAAAATAACATATT

Downstream 100 bases:

>100_bases
ATTTGTCTCTGTTCAGCAGAATAGCAAAATAAGGTAGCCAAGAAAAGACAATTATAGGAACAAATGGATATCATTAAAGC
AGCTGACACTGTGGCAGAAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKHKSEFLNFIQERGYLYQCTNIDRLDQLLSQDNYIIAYIGFDCTAPSLHIGSLIQIMMLRHLQKFGYKPIVLLGGGTTK
IGDPSGKDKARSVLPIEDINQNILGIKKTLEKMISFDYGKTGAIIVNNADWLDNIKYIDFLRDIGTHFSVNRMLGFDSVK
IRLDREQNLSFLEFNYMLLQAYDFVELNKKYGCRFQIGGSDQWGNIVNGIELSKKLNLPELFGLTTPLLLNAQGKKMGKT
ESGAVWLDGSMLNSYDYWQYFRNVDDQDVGRFLRLFTDLPIDEIKKLESLKDQETNEAKKVLATEVTKICHGCKEAELAR
SAAISAFENEDSSLLSGYTITKEQIANGIPLIDLLYDTGFEPSKGAAKRLIQGNGCKVNDNTINDVNYTINSESFKGQPF
IKLSAGKKRHIKILVSEVRK

Sequences:

>Translated_420_residues
MKHKSEFLNFIQERGYLYQCTNIDRLDQLLSQDNYIIAYIGFDCTAPSLHIGSLIQIMMLRHLQKFGYKPIVLLGGGTTK
IGDPSGKDKARSVLPIEDINQNILGIKKTLEKMISFDYGKTGAIIVNNADWLDNIKYIDFLRDIGTHFSVNRMLGFDSVK
IRLDREQNLSFLEFNYMLLQAYDFVELNKKYGCRFQIGGSDQWGNIVNGIELSKKLNLPELFGLTTPLLLNAQGKKMGKT
ESGAVWLDGSMLNSYDYWQYFRNVDDQDVGRFLRLFTDLPIDEIKKLESLKDQETNEAKKVLATEVTKICHGCKEAELAR
SAAISAFENEDSSLLSGYTITKEQIANGIPLIDLLYDTGFEPSKGAAKRLIQGNGCKVNDNTINDVNYTINSESFKGQPF
IKLSAGKKRHIKILVSEVRK
>Mature_420_residues
MKHKSEFLNFIQERGYLYQCTNIDRLDQLLSQDNYIIAYIGFDCTAPSLHIGSLIQIMMLRHLQKFGYKPIVLLGGGTTK
IGDPSGKDKARSVLPIEDINQNILGIKKTLEKMISFDYGKTGAIIVNNADWLDNIKYIDFLRDIGTHFSVNRMLGFDSVK
IRLDREQNLSFLEFNYMLLQAYDFVELNKKYGCRFQIGGSDQWGNIVNGIELSKKLNLPELFGLTTPLLLNAQGKKMGKT
ESGAVWLDGSMLNSYDYWQYFRNVDDQDVGRFLRLFTDLPIDEIKKLESLKDQETNEAKKVLATEVTKICHGCKEAELAR
SAAISAFENEDSSLLSGYTITKEQIANGIPLIDLLYDTGFEPSKGAAKRLIQGNGCKVNDNTINDVNYTINSESFKGQPF
IKLSAGKKRHIKILVSEVRK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=367, Percent_Identity=34.6049046321526, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1787925, Length=420, Percent_Identity=40.7142857142857, Blast_Score=309, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25145802, Length=303, Percent_Identity=37.953795379538, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25145805, Length=217, Percent_Identity=37.3271889400922, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6325160, Length=349, Percent_Identity=35.243553008596, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI20130383, Length=410, Percent_Identity=33.9024390243902, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_WOLPP (B3CM50)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975618.1
- EnsemblBacteria:   EBWOLT00000000552
- GeneID:   6384655
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0861
- GeneTree:   EBGT00050000031319
- HOGENOM:   HBG288125
- OMA:   DRIDNQL
- ProtClustDB:   PRK13354
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47509; Mature: 47509

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 39-39 BINDING 176-176 BINDING 180-180 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKSEFLNFIQERGYLYQCTNIDRLDQLLSQDNYIIAYIGFDCTAPSLHIGSLIQIMML
CCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
RHLQKFGYKPIVLLGGGTTKIGDPSGKDKARSVLPIEDINQNILGIKKTLEKMISFDYGK
HHHHHCCCCEEEEECCCCCCCCCCCCCCHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCCC
TGAIIVNNADWLDNIKYIDFLRDIGTHFSVNRMLGFDSVKIRLDREQNLSFLEFNYMLLQ
CCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHCCCCEEEEEEECCCCCCEEEHHHHHHH
AYDFVELNKKYGCRFQIGGSDQWGNIVNGIELSKKLNLPELFGLTTPLLLNAQGKKMGKT
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHCCCCCEEEECCCCCCCCC
ESGAVWLDGSMLNSYDYWQYFRNVDDQDVGRFLRLFTDLPIDEIKKLESLKDQETNEAKK
CCCEEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VLATEVTKICHGCKEAELARSAAISAFENEDSSLLSGYTITKEQIANGIPLIDLLYDTGF
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCEEEEHHHHHCCCCEEEHHHHCCC
EPSKGAAKRLIQGNGCKVNDNTINDVNYTINSESFKGQPFIKLSAGKKRHIKILVSEVRK
CCCCHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKHKSEFLNFIQERGYLYQCTNIDRLDQLLSQDNYIIAYIGFDCTAPSLHIGSLIQIMML
CCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
RHLQKFGYKPIVLLGGGTTKIGDPSGKDKARSVLPIEDINQNILGIKKTLEKMISFDYGK
HHHHHCCCCEEEEECCCCCCCCCCCCCCHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCCC
TGAIIVNNADWLDNIKYIDFLRDIGTHFSVNRMLGFDSVKIRLDREQNLSFLEFNYMLLQ
CCEEEEECCHHHHHHHHHHHHHHHCCCEEHHHHCCCCEEEEEEECCCCCCEEEHHHHHHH
AYDFVELNKKYGCRFQIGGSDQWGNIVNGIELSKKLNLPELFGLTTPLLLNAQGKKMGKT
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHHHCCCCCEEEECCCCCCCCC
ESGAVWLDGSMLNSYDYWQYFRNVDDQDVGRFLRLFTDLPIDEIKKLESLKDQETNEAKK
CCCEEEECCCHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VLATEVTKICHGCKEAELARSAAISAFENEDSSLLSGYTITKEQIANGIPLIDLLYDTGF
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHCCEEEEHHHHHCCCCEEEHHHHCCC
EPSKGAAKRLIQGNGCKVNDNTINDVNYTINSESFKGQPFIKLSAGKKRHIKILVSEVRK
CCCCHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA