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The map label for this gene is rppH

Identifier: 190571256

GI number: 190571256

Start: 930612

End: 931100

Strand: Reverse

Name: rppH

Synonym: WPa_0857

Alternate gene names: 190571256

Gene position: 931100-930612 (Counterclockwise)

Preceding gene: 190571257

Following gene: 190571255

Centisome position: 62.81

GC content: 37.63

Gene sequence:

>489_bases
GTGATTAGTGAGGAGAAAGAGTATCGCCCTTGTGTTGGCATAATGTTATTTAACAAACAGGGAAATATTTTTATTGGAAA
ACGTTTTGATAGTGACTCTTATTGGCAGATGCCACAAGGAGGAGTTGATGAAGGTGAAGAGCTAGAGCAGGCAGCGCTAC
GTGAGCTATTGGAGGAAGTTGGTACTGATGAAGCAGAAGTTGTGGCTCAAAATAAAGAGTGGATATATTACAACTTACCC
GAGGAAGTTATACCGATATGCTGGAATGGGAGATATTCTGGCCAAAAGCAAAGGTGGTTCTTAATGAAATTTTGTGGGAA
GGATAAGGATATTAATATTAACTATACTGATCATCCAGAGTTCAAAGAGTGGCGTTGGCAAAATGTGGATGATTTGGTAG
CCAGTGCTATACCGTTCAAAAAAGAAGTTTATAAAAAAGTGATAGAAGAGTTTTCTTCTATCATAAAAGGATCTATTTAT
GATAGTTGA

Upstream 100 bases:

>100_bases
TAAGCGCAAAAGAAGTTGCAAAAAAAAATTGTTGAAAAAATGAATAGTAAAAAAGTAGTTGGAACTTCTATGATAGATAA
TTTAGCAACAGAGAGTATTT

Downstream 100 bases:

>100_bases
TTCTCATTGTCATCTAATTTATTTTTCTGATGATGAAATACCAAAGGTAATTTCAAGAGCAGAGCAAAATGGTGTGAGAA
TTTTGCATAACATATGTATA

Product: dinucleoside polyphosphate hydrolase

Products: NA

Alternate protein names: (Di)nucleoside polyphosphate hydrolase

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MISEEKEYRPCVGIMLFNKQGNIFIGKRFDSDSYWQMPQGGVDEGEELEQAALRELLEEVGTDEAEVVAQNKEWIYYNLP
EEVIPICWNGRYSGQKQRWFLMKFCGKDKDININYTDHPEFKEWRWQNVDDLVASAIPFKKEVYKKVIEEFSSIIKGSIY
DS

Sequences:

>Translated_162_residues
MISEEKEYRPCVGIMLFNKQGNIFIGKRFDSDSYWQMPQGGVDEGEELEQAALRELLEEVGTDEAEVVAQNKEWIYYNLP
EEVIPICWNGRYSGQKQRWFLMKFCGKDKDININYTDHPEFKEWRWQNVDDLVASAIPFKKEVYKKVIEEFSSIIKGSIY
DS
>Mature_162_residues
MISEEKEYRPCVGIMLFNKQGNIFIGKRFDSDSYWQMPQGGVDEGEELEQAALRELLEEVGTDEAEVVAQNKEWIYYNLP
EEVIPICWNGRYSGQKQRWFLMKFCGKDKDININYTDHPEFKEWRWQNVDDLVASAIPFKKEVYKKVIEEFSSIIKGSIY
DS

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1789194, Length=155, Percent_Identity=40.6451612903226, Blast_Score=129, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPPH_WOLPP (B3CM46)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975614.1
- ProteinModelPortal:   B3CM46
- EnsemblBacteria:   EBWOLT00000000188
- GeneID:   6384651
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0857
- GeneTree:   EBGT00050000031465
- HOGENOM:   HBG302451
- OMA:   GQKQIWY
- ProtClustDB:   PRK00714
- HAMAP:   MF_00298
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.1.- [C]

Molecular weight: Translated: 19096; Mature: 19096

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEEKEYRPCVGIMLFNKQGNIFIGKRFDSDSYWQMPQGGVDEGEELEQAALRELLEEV
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
GTDEAEVVAQNKEWIYYNLPEEVIPICWNGRYSGQKQRWFLMKFCGKDKDININYTDHPE
CCCCHHEEECCCCEEEEECCHHHEEEEECCCCCCCCHHHHHHHHCCCCCCEEECCCCCCC
FKEWRWQNVDDLVASAIPFKKEVYKKVIEEFSSIIKGSIYDS
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MISEEKEYRPCVGIMLFNKQGNIFIGKRFDSDSYWQMPQGGVDEGEELEQAALRELLEEV
CCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
GTDEAEVVAQNKEWIYYNLPEEVIPICWNGRYSGQKQRWFLMKFCGKDKDININYTDHPE
CCCCHHEEECCCCEEEEECCHHHEEEEECCCCCCCCHHHHHHHHCCCCCCEEECCCCCCC
FKEWRWQNVDDLVASAIPFKKEVYKKVIEEFSSIIKGSIYDS
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA