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The map label for this gene is tilS

Identifier: 190571198

GI number: 190571198

Start: 875270

End: 876562

Strand: Reverse

Name: tilS

Synonym: WPa_0796

Alternate gene names: 190571198

Gene position: 876562-875270 (Counterclockwise)

Preceding gene: 190571199

Following gene: 190571197

Centisome position: 59.13

GC content: 32.02

Gene sequence:

>1293_bases
ATGAAATTAGAGTTATTATTTCAAAATATAGCTAATAGTTTTGCTTTCCATAACCAAATCGCAGTTGCAGTATCAGGTGG
TGTAGATAGTATAGTCTTACTGCATTTAATGACTAACTGGGCAAAAAAAAACAAGCTTTCACTTCCTATAGCATTAACAG
TAAATCATGGGTTACGTTCAGAGTCTCAAAAAGAAGCTGATTTTATTATAAGTTATGCAAAAGAACTTGGAGCAAAGGAA
TCGTTCATATTAAATTGGGAGAAGCAAAATATTAAAGGTAATATTCAGTTACAGGCACGAAAAGCACGATATAAGTTACT
AGCAGAGTGGTGTAAAAACAATAATGTTAAATGTTTGCTCGTTGCTCATCACAAAGATGATCAAGCAGAAACGTTCTTAT
TAAGATTGGAGCGAGGTAGTGGAGTAGATGGATTATCATCAATGGATTACAAATCTTTCTTAAATGGTATTTATATATTT
AGGCCGTTGTTAAATTTTAGTCGTAGTGAAATAGAAAGGTATGCTAAGCTTCATCAGTTAAGATGGATCGAAGATAGAAG
CAATTATGACTTAAAATACAGACGAACTTTATACCGTAACTTACTTAAAGCAAGTGATAATCAAGATGTTCTAACAGAGC
GAATATGCCTTACAGCACTTCATATGAAAAGAGCTGCAAAAGCGTTGATGCACTACACACGCCTTGCATTTGATGATTGT
GTTAATGTTCATGATTTTGGTTATATTGAAATTAAACTAAGTGAATTTTATCAATTGCCAGAGGAAATAGCCTTGAGGCT
TCTTCTTTACTCTATAATGGCAATTGCCAGCAAACATTATAAACCAAGGTACAACAGCCTTATCGTAATATTTAATAAAA
TATTGCAAAAGGGTAGTAATGTTAACTGTACACTTTCTGGGTGCAAAATAAGAAAATATGGAGAAAATATCTTAATAATT
AGAGAATCGTCAAAGATACAAGAAATTACTGTACACTTACCATTAAATGGTTCTATTGAATGGGATAACAGGTTTAGCTG
CACAATATTTGGAGATCAGGAGTGTTCAGTAACTATTGCTCCACTAAAGAAAACACAAAAAATCCCTGAATTTCTAAAAA
ATTATGATTATTGCTCTGAAGTTTACTATTCTTTGCCTACAGTACAAAAAGATGGAAAGATGCTTGCTTATCCTGATGTA
AATTATAACGGAAAAAATACCGATGATGATAAGGTTCGATTCATTATTAATAGCACGATAAAACAAAATTTGGTAAGCTT
GATTAGTATTTAA

Upstream 100 bases:

>100_bases
TGACAAAAGAGAGCAATTCCGCTGCTTGTTAGCGGGATCTATATATACCACAAATAAGTCGCTGTATGACGTAGAGACTA
TCTGGCCATCAAGAAACATA

Downstream 100 bases:

>100_bases
TTAGAAATGTAAATGAAAAAATTTTTAGAAGGCTTATTGATCTGGTTAGTGATTATTGTTCTTATTTCAGTTGCTTATAT
TCAATTTAGCGGAAGTATAG

Product: tRNA(ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKLELLFQNIANSFAFHNQIAVAVSGGVDSIVLLHLMTNWAKKNKLSLPIALTVNHGLRSESQKEADFIISYAKELGAKE
SFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKCLLVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIF
RPLLNFSRSEIERYAKLHQLRWIEDRSNYDLKYRRTLYRNLLKASDNQDVLTERICLTALHMKRAAKALMHYTRLAFDDC
VNVHDFGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKGSNVNCTLSGCKIRKYGENILII
RESSKIQEITVHLPLNGSIEWDNRFSCTIFGDQECSVTIAPLKKTQKIPEFLKNYDYCSEVYYSLPTVQKDGKMLAYPDV
NYNGKNTDDDKVRFIINSTIKQNLVSLISI

Sequences:

>Translated_430_residues
MKLELLFQNIANSFAFHNQIAVAVSGGVDSIVLLHLMTNWAKKNKLSLPIALTVNHGLRSESQKEADFIISYAKELGAKE
SFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKCLLVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIF
RPLLNFSRSEIERYAKLHQLRWIEDRSNYDLKYRRTLYRNLLKASDNQDVLTERICLTALHMKRAAKALMHYTRLAFDDC
VNVHDFGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKGSNVNCTLSGCKIRKYGENILII
RESSKIQEITVHLPLNGSIEWDNRFSCTIFGDQECSVTIAPLKKTQKIPEFLKNYDYCSEVYYSLPTVQKDGKMLAYPDV
NYNGKNTDDDKVRFIINSTIKQNLVSLISI
>Mature_430_residues
MKLELLFQNIANSFAFHNQIAVAVSGGVDSIVLLHLMTNWAKKNKLSLPIALTVNHGLRSESQKEADFIISYAKELGAKE
SFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKCLLVAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIF
RPLLNFSRSEIERYAKLHQLRWIEDRSNYDLKYRRTLYRNLLKASDNQDVLTERICLTALHMKRAAKALMHYTRLAFDDC
VNVHDFGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKGSNVNCTLSGCKIRKYGENILII
RESSKIQEITVHLPLNGSIEWDNRFSCTIFGDQECSVTIAPLKKTQKIPEFLKNYDYCSEVYYSLPTVQKDGKMLAYPDV
NYNGKNTDDDKVRFIINSTIKQNLVSLISI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=184, Percent_Identity=40.2173913043478, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_WOLPP (B3CLY6)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975556.1
- EnsemblBacteria:   EBWOLT00000001144
- GeneID:   6384640
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0796
- GeneTree:   EBGT00050000031233
- HOGENOM:   HBG311245
- OMA:   NIQIIRP
- ProtClustDB:   CLSK740062
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 49677; Mature: 49677

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLELLFQNIANSFAFHNQIAVAVSGGVDSIVLLHLMTNWAKKNKLSLPIALTVNHGLRS
CCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ESQKEADFIISYAKELGAKESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKCLL
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCCEEEE
VAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIFRPLLNFSRSEIERYAKLHQL
EEECCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RWIEDRSNYDLKYRRTLYRNLLKASDNQDVLTERICLTALHMKRAAKALMHYTRLAFDDC
HEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VNVHDFGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKGSN
CCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
VNCTLSGCKIRKYGENILIIRESSKIQEITVHLPLNGSIEWDNRFSCTIFGDQECSVTIA
CEEEEECCEEEECCCEEEEEECCCCEEEEEEEECCCCCEEECCEEEEEEECCCCCEEEEE
PLKKTQKIPEFLKNYDYCSEVYYSLPTVQKDGKMLAYPDVNYNGKNTDDDKVRFIINSTI
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEHHHH
KQNLVSLISI
HHHHHHHHCC
>Mature Secondary Structure
MKLELLFQNIANSFAFHNQIAVAVSGGVDSIVLLHLMTNWAKKNKLSLPIALTVNHGLRS
CCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ESQKEADFIISYAKELGAKESFILNWEKQNIKGNIQLQARKARYKLLAEWCKNNNVKCLL
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHCCCCCEEEE
VAHHKDDQAETFLLRLERGSGVDGLSSMDYKSFLNGIYIFRPLLNFSRSEIERYAKLHQL
EEECCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RWIEDRSNYDLKYRRTLYRNLLKASDNQDVLTERICLTALHMKRAAKALMHYTRLAFDDC
HEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VNVHDFGYIEIKLSEFYQLPEEIALRLLLYSIMAIASKHYKPRYNSLIVIFNKILQKGSN
CCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
VNCTLSGCKIRKYGENILIIRESSKIQEITVHLPLNGSIEWDNRFSCTIFGDQECSVTIA
CEEEEECCEEEECCCEEEEEECCCCEEEEEEEECCCCCEEECCEEEEEEECCCCCEEEEE
PLKKTQKIPEFLKNYDYCSEVYYSLPTVQKDGKMLAYPDVNYNGKNTDDDKVRFIINSTI
CCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEHHHH
KQNLVSLISI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA