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The map label for this gene is purK [H]

Identifier: 190571121

GI number: 190571121

Start: 784228

End: 785307

Strand: Direct

Name: purK [H]

Synonym: WPa_0719

Alternate gene names: 190571121

Gene position: 784228-785307 (Clockwise)

Preceding gene: 190571119

Following gene: 190571123

Centisome position: 52.9

GC content: 35.09

Gene sequence:

>1080_bases
ATGATGAACAAAACAGATCCACTGGATAAAAAAGTGATAGGAATAATAGGAAGTGGGCAATTAGGTAAAATGACTGCTAT
CGCTGCAATAAAACTTGGGCACAAAACGCATATTTTTGCCAGTGCTAAAAACGATCCAGCTTGTTCTGTTGCTGATGATT
TAACAATAGCAGACTTCTGTGATAAGAGGGCACTTGAATCTTTTGCACAGAGTGTGGATTTAGTCACTATTGAGTCTGAA
AATATTCCATGTAGTGCAATTGATATTGTGTCGCAGCATACAGATTTTTATCCAGGTAAAAAGGCGTTACACATTTCGCA
AAATAGGCTGAGAGAGAAAGATTTTATTAGAGATTTAAGCATAAAAACTGCTGATTACAAAAGTATACAAAATTATAATC
AGCTACTAGAAAGTAGTGAAACTTTTAGCTATCCAGTGAGGCTAAAAACAACAGAAATGGGTTATGATGGAAAAGGACAA
TATATGATTGAAAATGATTCTGAACTGAAGCAATTTGCTTCCTTCGATTGGAATAAAGAGTACATTCTTGAAGCAAGTGT
TGATTTACTGAAAGAGGTTTCAATAGTCGTTGCAAGAGATAAAAACGGTAAAGTAGCTTTTTTTCCTATAGCAGAGAATC
ACCACGTTGACGGAATACTTAATACTTCAATAGCACCAGCTAAAATAGATAATAAATTAGCCCAAGAGGTACAAAAAACT
GCAGAGAAAATAGCAACTGCACTTGATGTAGTAGGAATTCTAGCTATTGAATTTTTCATCACTCAAAATCATGAATTACT
AGTTAATGAACTGGCCCCAAGACCCCACAATTCTTGCCACTGGAGCTTGGATGCATGCAACGTTAGTCAATTCGAGCAGC
TAGTTAGGATAATATGCGGGCTATCTATGCAGGAAGTAGTATTACGCTTTCCTTGCATGACAAAAAATATAATAGGTAAT
GATATATATAATTCTCACAAATATTTGAGCAACGAAAAAGCTAGTTTAACCATATATGGGAAAAAAGAGGTTAGGGACAA
GCGTAAAATGGGACATGTCAATATAGATTTAAGCTATTGA

Upstream 100 bases:

>100_bases
AAAGTGTTCATGATAAAAGTTTAACACACTTCAATTATACACTTTTTTCATAGATTTTCCAAGCATATTATCGTACTCTA
ACTAGGAAATATTAATCTAC

Downstream 100 bases:

>100_bases
GCTATAAATGCTCTTTGATCAAGATCTCCAAGATCTGCACTATATTTAGTGCTGCACCTTTGCGTAGATTATCAGCTACT
ATCCACATATTTAATCCGTG

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MMNKTDPLDKKVIGIIGSGQLGKMTAIAAIKLGHKTHIFASAKNDPACSVADDLTIADFCDKRALESFAQSVDLVTIESE
NIPCSAIDIVSQHTDFYPGKKALHISQNRLREKDFIRDLSIKTADYKSIQNYNQLLESSETFSYPVRLKTTEMGYDGKGQ
YMIENDSELKQFASFDWNKEYILEASVDLLKEVSIVVARDKNGKVAFFPIAENHHVDGILNTSIAPAKIDNKLAQEVQKT
AEKIATALDVVGILAIEFFITQNHELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLSMQEVVLRFPCMTKNIIGN
DIYNSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLSY

Sequences:

>Translated_359_residues
MMNKTDPLDKKVIGIIGSGQLGKMTAIAAIKLGHKTHIFASAKNDPACSVADDLTIADFCDKRALESFAQSVDLVTIESE
NIPCSAIDIVSQHTDFYPGKKALHISQNRLREKDFIRDLSIKTADYKSIQNYNQLLESSETFSYPVRLKTTEMGYDGKGQ
YMIENDSELKQFASFDWNKEYILEASVDLLKEVSIVVARDKNGKVAFFPIAENHHVDGILNTSIAPAKIDNKLAQEVQKT
AEKIATALDVVGILAIEFFITQNHELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLSMQEVVLRFPCMTKNIIGN
DIYNSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLSY
>Mature_359_residues
MMNKTDPLDKKVIGIIGSGQLGKMTAIAAIKLGHKTHIFASAKNDPACSVADDLTIADFCDKRALESFAQSVDLVTIESE
NIPCSAIDIVSQHTDFYPGKKALHISQNRLREKDFIRDLSIKTADYKSIQNYNQLLESSETFSYPVRLKTTEMGYDGKGQ
YMIENDSELKQFASFDWNKEYILEASVDLLKEVSIVVARDKNGKVAFFPIAENHHVDGILNTSIAPAKIDNKLAQEVQKT
AEKIATALDVVGILAIEFFITQNHELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICGLSMQEVVLRFPCMTKNIIGN
DIYNSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLSY

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=346, Percent_Identity=30.3468208092486, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788155, Length=360, Percent_Identity=22.7777777777778, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=29.5081967213115, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40279; Mature: 40279

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNKTDPLDKKVIGIIGSGQLGKMTAIAAIKLGHKTHIFASAKNDPACSVADDLTIADFC
CCCCCCCCCCEEEEEECCCCCCCHHEEEEEEECCEEEEEEECCCCCCCCCCCCCCHHHHH
DKRALESFAQSVDLVTIESENIPCSAIDIVSQHTDFYPGKKALHISQNRLREKDFIRDLS
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCC
IKTADYKSIQNYNQLLESSETFSYPVRLKTTEMGYDGKGQYMIENDSELKQFASFDWNKE
CCCCHHHHHHHHHHHHHCCCCEECEEEEEEEECCCCCCCCEEEECCHHHHHHHCCCCCHH
YILEASVDLLKEVSIVVARDKNGKVAFFPIAENHHVDGILNTSIAPAKIDNKLAQEVQKT
EEEHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHH
AEKIATALDVVGILAIEFFITQNHELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICG
HHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHC
LSMQEVVLRFPCMTKNIIGNDIYNSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLSY
CCHHHHHHHCCCHHHHHHCCCHHCCHHHHCCCCCEEEEEECHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MMNKTDPLDKKVIGIIGSGQLGKMTAIAAIKLGHKTHIFASAKNDPACSVADDLTIADFC
CCCCCCCCCCEEEEEECCCCCCCHHEEEEEEECCEEEEEEECCCCCCCCCCCCCCHHHHH
DKRALESFAQSVDLVTIESENIPCSAIDIVSQHTDFYPGKKALHISQNRLREKDFIRDLS
HHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCC
IKTADYKSIQNYNQLLESSETFSYPVRLKTTEMGYDGKGQYMIENDSELKQFASFDWNKE
CCCCHHHHHHHHHHHHHCCCCEECEEEEEEEECCCCCCCCEEEECCHHHHHHHCCCCCHH
YILEASVDLLKEVSIVVARDKNGKVAFFPIAENHHVDGILNTSIAPAKIDNKLAQEVQKT
EEEHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHH
AEKIATALDVVGILAIEFFITQNHELLVNELAPRPHNSCHWSLDACNVSQFEQLVRIICG
HHHHHHHHHHHHHHHHHHEEECCCHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHC
LSMQEVVLRFPCMTKNIIGNDIYNSHKYLSNEKASLTIYGKKEVRDKRKMGHVNIDLSY
CCHHHHHHHCCCHHHHHHCCCHHCCHHHHCCCCCEEEEEECHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]