Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 190571057

GI number: 190571057

Start: 714330

End: 714947

Strand: Direct

Name: lipB

Synonym: WPa_0650

Alternate gene names: 190571057

Gene position: 714330-714947 (Clockwise)

Preceding gene: 190571054

Following gene: 190571058

Centisome position: 48.19

GC content: 33.66

Gene sequence:

>618_bases
ATGACAGAATGGCTAATCTCTAACCAATTTACTGATTATAACCATTCTGTGAAATTCATGGAAGCAAAAATTCAACAAAT
TTACAATAATTTGTCTGATGAGCTAGTATGGCTGCTCCAGCACCCTCCACTTTACACAGCAGGAATCGGTGCAACAGATG
ATGACATTATTGAAAAATTATTTCCTATATATAAAACAGGTAGGGGTGGTAAATATACATATCACGGACCAGGGCAGCGC
ATTATATATTTAATGCTAAATCTTAAAAAAAGAAACAAATGTGACATAAAACTATATATCAGAGACCTAAGTAATTGGAT
CATAAATGTTTTAAAACACTTTAATATACTTGGGGAATTTAAGGAAGATAGAATAGGCATTTGGGTGAACAACAAAGGAG
TAGAAGAAAAAATAGCAGCTTTTGGCATTCGCTTGAGAAAATGGGTAACTTATCACGGCATAGCGCTTAATGTCTCTCCA
AACCTTTCCCACTATGAGGGTATTATTCCTTGTGGACTAAAAGATTACGGCGTTACATCAATGGAAAAACTAGGAGTAAA
AGTTTCTCTTTGCGAATTAGATGATATATTAAAGCAAGAGTTTCATAAGATATTTTAA

Upstream 100 bases:

>100_bases
AGCAACTATGCTCACCAGTTCAGTGTGCTATCTACAATTAAATTTCTGGATTCCAGTGTCAAGCGCTGGAATGACATTGA
TAGACCTAAAGAAATAGAAC

Downstream 100 bases:

>100_bases
TGTATTCCTTTATTCTTTATTTTCTATGCCTTTTACTCACTAATCCTGCAAATCAAGTTGATATTATTGCTCCTTCTTCG
AAAGGAAAAGAATCAGATCT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTEWLISNQFTDYNHSVKFMEAKIQQIYNNLSDELVWLLQHPPLYTAGIGATDDDIIEKLFPIYKTGRGGKYTYHGPGQR
IIYLMLNLKKRNKCDIKLYIRDLSNWIINVLKHFNILGEFKEDRIGIWVNNKGVEEKIAAFGIRLRKWVTYHGIALNVSP
NLSHYEGIIPCGLKDYGVTSMEKLGVKVSLCELDDILKQEFHKIF

Sequences:

>Translated_205_residues
MTEWLISNQFTDYNHSVKFMEAKIQQIYNNLSDELVWLLQHPPLYTAGIGATDDDIIEKLFPIYKTGRGGKYTYHGPGQR
IIYLMLNLKKRNKCDIKLYIRDLSNWIINVLKHFNILGEFKEDRIGIWVNNKGVEEKIAAFGIRLRKWVTYHGIALNVSP
NLSHYEGIIPCGLKDYGVTSMEKLGVKVSLCELDDILKQEFHKIF
>Mature_204_residues
TEWLISNQFTDYNHSVKFMEAKIQQIYNNLSDELVWLLQHPPLYTAGIGATDDDIIEKLFPIYKTGRGGKYTYHGPGQRI
IYLMLNLKKRNKCDIKLYIRDLSNWIINVLKHFNILGEFKEDRIGIWVNNKGVEEKIAAFGIRLRKWVTYHGIALNVSPN
LSHYEGIIPCGLKDYGVTSMEKLGVKVSLCELDDILKQEFHKIF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI87081767, Length=150, Percent_Identity=35.3333333333333, Blast_Score=104, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323268, Length=123, Percent_Identity=34.1463414634146, Blast_Score=78, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355483, Length=193, Percent_Identity=33.160621761658, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_WOLPP (B3CLJ0)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975415.1
- EnsemblBacteria:   EBWOLT00000000531
- GeneID:   6385262
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0650
- GeneTree:   EBGT00050000031072
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   PRK14341
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23823; Mature: 23692

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEWLISNQFTDYNHSVKFMEAKIQQIYNNLSDELVWLLQHPPLYTAGIGATDDDIIEKL
CCCCEECCCCCCCCCHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEECCCCCCHHHHHHH
FPIYKTGRGGKYTYHGPGQRIIYLMLNLKKRNKCDIKLYIRDLSNWIINVLKHFNILGEF
HHHHCCCCCCCEEEECCCCEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
KEDRIGIWVNNKGVEEKIAAFGIRLRKWVTYHGIALNVSPNLSHYEGIIPCGLKDYGVTS
CCCEEEEEECCCCHHHHHHHHHHHHHHEEEECCEEEEECCCCHHHCCCCCCCCCCCCCCH
MEKLGVKVSLCELDDILKQEFHKIF
HHHCCCEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEWLISNQFTDYNHSVKFMEAKIQQIYNNLSDELVWLLQHPPLYTAGIGATDDDIIEKL
CCCEECCCCCCCCCHHHHHHHHHHHHHHCHHHHEEEEECCCCEEEECCCCCCHHHHHHH
FPIYKTGRGGKYTYHGPGQRIIYLMLNLKKRNKCDIKLYIRDLSNWIINVLKHFNILGEF
HHHHCCCCCCCEEEECCCCEEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
KEDRIGIWVNNKGVEEKIAAFGIRLRKWVTYHGIALNVSPNLSHYEGIIPCGLKDYGVTS
CCCEEEEEECCCCHHHHHHHHHHHHHHEEEECCEEEEECCCCHHHCCCCCCCCCCCCCCH
MEKLGVKVSLCELDDILKQEFHKIF
HHHCCCEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA