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The map label for this gene is murE [H]

Identifier: 190570599

GI number: 190570599

Start: 143062

End: 144624

Strand: Reverse

Name: murE [H]

Synonym: WPa_0137

Alternate gene names: 190570599

Gene position: 144624-143062 (Counterclockwise)

Preceding gene: 190570600

Following gene: 190570596

Centisome position: 9.76

GC content: 37.75

Gene sequence:

>1563_bases
ATGATTGTGAGACTGAAAGAGCTACTGCATAACATTGTAAATGCTGACTTTGATATTGAAATCAAAGGCGTCACATGTAA
TCCTAAGAGAGTTAAAGAAGGTTACCTTTTTGTTTGTGTGTCAGAAAGGAGTAAAGAACACGTAACATGTCTTCCTAATG
GATGTGTACACAACTGTGTAGACGTTGTAATACAAGGGGCCAAGAAAGATGTCATCCCAGTGCTCAGACACTGGGATCCA
GAAAATTTAATTGTAAACAAGCATACTAGACAATTGTATAATGAAAACTGGATTCCAGTGTCCGCTACTCGAATGACAAG
TACTCCAAGTTTTGCAGTGCTTTACCACTCGGACCCTCAAGGAATATACAGTGAAATAGTCAGCAGGTTTTATCAATTCA
AACAACCTAAATATGTTGCTGCTGTAACTGGTACGAATGGTAAAACTTCAGTGGTGGAATTTTGTCGCCAGATTTGGCAA
GATAATGTAGCGTCTATAGGAACACTGGGAACATGTGTCAATAATAATAGAAAAGATAATAGCCTTACAACGCCAAGTGC
AGATGATCTTTATTCCACATTGCGTGACATAAATAATAAGAACGTAGAACACTTAGCATTAGAAGCTTCAAGCCATGGAA
TCGATCAATACAGAATCCATGGACTCAGGCTCAATGCTGCAGCTTTTACTAATTTCTCTCAAGATCATTTGGACTATCAC
AAAAGTATCGATGAGTATTTTGAGGCCAAGAAAAGGTTGTTTGATGAGGTATTGCCAAAAGAAAAAACAGCGATTTTAAA
TGCAGATATAGGCGAATATGGAGAGTTGCTCAACATAGCTGAAAAGCGTGGCAACAAAGTTATAACCTATGGAAAAAAAA
ACTCTGACATTACTTTATTAAAGCAGATACCCACTGCCAATGGTCAATACCTGACAATTCAAATCGGTGATGAAATTTAT
AACGCATTTTTTCCGATTTTTGGGCAATTTCAAGCATATAATTTGTTGTGTGCAATCGGTATAGTTATTTCATCTGGTAT
GGACTATCAAAGAATGTGTATAGATAAACTCGCTTCTCCATCTGGAAGAATGGAAAAAGTGAACACTTTTGCATTTGTCG
ATTACGCTCATACTCCAAGTGCACTCAAACAAGCTCTGTTGTCTTTAAAATGGCACTTCAATAAAAAGATAGTTCTAGTG
TTCGGTTGTGGCGGGAATCGTGATCAAACAAAACGTGCAGAGATGGGTAAAATAGCACAAATGCTTGCAGGTAAAGTTAT
AGTCACAGATGACAATCCGCGCGATGAAGATCCGGGGAAAATTCGTCACGATATTTTGTTACATTGCCCTGATGCACTGG
AGATAGGAGATAGAAAAAAAGCTATAGAGAAAGGTATAAATATAGCACATGATGAAAATATGATTCTGCTAGTTGCAGGA
AAAGGACATGAAAGATTCCAAATAATAGAAGGCCAATCCCTTGAATTTAGTGATGTTGAAGTTATTAGAGAGGGCCAAAA
ATTTGCTACTGCTGTTTTGCTCTCTCCTTCATCCAACGCCTGA

Upstream 100 bases:

>100_bases
GGAAGTTTTGTGCGAATATTTTGCGGACTAGAAGATCCTGAAGATTTGATTTTTGACTTGAATACTGCATTAATAAGACT
GCCATGTTCAAGTGTTAAAG

Downstream 100 bases:

>100_bases
ATGGCTTTTTTCCTTAAGTCATAACTGTACGCTGCTGGCATTTTTACCTTCTTGTTACACTAAATCACTATCTTATCTTG
TTCCTACTATTATGAGAAGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MIVRLKELLHNIVNADFDIEIKGVTCNPKRVKEGYLFVCVSERSKEHVTCLPNGCVHNCVDVVIQGAKKDVIPVLRHWDP
ENLIVNKHTRQLYNENWIPVSATRMTSTPSFAVLYHSDPQGIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQ
DNVASIGTLGTCVNNNRKDNSLTTPSADDLYSTLRDINNKNVEHLALEASSHGIDQYRIHGLRLNAAAFTNFSQDHLDYH
KSIDEYFEAKKRLFDEVLPKEKTAILNADIGEYGELLNIAEKRGNKVITYGKKNSDITLLKQIPTANGQYLTIQIGDEIY
NAFFPIFGQFQAYNLLCAIGIVISSGMDYQRMCIDKLASPSGRMEKVNTFAFVDYAHTPSALKQALLSLKWHFNKKIVLV
FGCGGNRDQTKRAEMGKIAQMLAGKVIVTDDNPRDEDPGKIRHDILLHCPDALEIGDRKKAIEKGINIAHDENMILLVAG
KGHERFQIIEGQSLEFSDVEVIREGQKFATAVLLSPSSNA

Sequences:

>Translated_520_residues
MIVRLKELLHNIVNADFDIEIKGVTCNPKRVKEGYLFVCVSERSKEHVTCLPNGCVHNCVDVVIQGAKKDVIPVLRHWDP
ENLIVNKHTRQLYNENWIPVSATRMTSTPSFAVLYHSDPQGIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQ
DNVASIGTLGTCVNNNRKDNSLTTPSADDLYSTLRDINNKNVEHLALEASSHGIDQYRIHGLRLNAAAFTNFSQDHLDYH
KSIDEYFEAKKRLFDEVLPKEKTAILNADIGEYGELLNIAEKRGNKVITYGKKNSDITLLKQIPTANGQYLTIQIGDEIY
NAFFPIFGQFQAYNLLCAIGIVISSGMDYQRMCIDKLASPSGRMEKVNTFAFVDYAHTPSALKQALLSLKWHFNKKIVLV
FGCGGNRDQTKRAEMGKIAQMLAGKVIVTDDNPRDEDPGKIRHDILLHCPDALEIGDRKKAIEKGINIAHDENMILLVAG
KGHERFQIIEGQSLEFSDVEVIREGQKFATAVLLSPSSNA
>Mature_520_residues
MIVRLKELLHNIVNADFDIEIKGVTCNPKRVKEGYLFVCVSERSKEHVTCLPNGCVHNCVDVVIQGAKKDVIPVLRHWDP
ENLIVNKHTRQLYNENWIPVSATRMTSTPSFAVLYHSDPQGIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQ
DNVASIGTLGTCVNNNRKDNSLTTPSADDLYSTLRDINNKNVEHLALEASSHGIDQYRIHGLRLNAAAFTNFSQDHLDYH
KSIDEYFEAKKRLFDEVLPKEKTAILNADIGEYGELLNIAEKRGNKVITYGKKNSDITLLKQIPTANGQYLTIQIGDEIY
NAFFPIFGQFQAYNLLCAIGIVISSGMDYQRMCIDKLASPSGRMEKVNTFAFVDYAHTPSALKQALLSLKWHFNKKIVLV
FGCGGNRDQTKRAEMGKIAQMLAGKVIVTDDNPRDEDPGKIRHDILLHCPDALEIGDRKKAIEKGINIAHDENMILLVAG
KGHERFQIIEGQSLEFSDVEVIREGQKFATAVLLSPSSNA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=359, Percent_Identity=33.7047353760446, Blast_Score=158, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 58375; Mature: 58375

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVRLKELLHNIVNADFDIEIKGVTCNPKRVKEGYLFVCVSERSKEHVTCLPNGCVHNCV
CEEHHHHHHHHHHCCCCEEEEEEEECCHHHCCCCEEEEEECCCCCCCEEECCCCHHHHHH
DVVIQGAKKDVIPVLRHWDPENLIVNKHTRQLYNENWIPVSATRMTSTPSFAVLYHSDPQ
HHHHCCCCCHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCH
GIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQDNVASIGTLGTCVNNNRKDN
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
SLTTPSADDLYSTLRDINNKNVEHLALEASSHGIDQYRIHGLRLNAAAFTNFSQDHLDYH
CCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEEEEHHHHCCCCHHHHHHH
KSIDEYFEAKKRLFDEVLPKEKTAILNADIGEYGELLNIAEKRGNKVITYGKKNSDITLL
HHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
KQIPTANGQYLTIQIGDEIYNAFFPIFGQFQAYNLLCAIGIVISSGMDYQRMCIDKLASP
EECCCCCCCEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
SGRMEKVNTFAFVDYAHTPSALKQALLSLKWHFNKKIVLVFGCGGNRDQTKRAEMGKIAQ
CCCCHHEEEEEEEEECCCHHHHHHHHHHHHEECCCEEEEEEECCCCCCHHHHHHHHHHHH
MLAGKVIVTDDNPRDEDPGKIRHDILLHCPDALEIGDRKKAIEKGINIAHDENMILLVAG
HHCCEEEEECCCCCCCCCCCEECCEEEECCCHHCCCCHHHHHHHCCCEEECCCEEEEEEC
KGHERFQIIEGQSLEFSDVEVIREGQKFATAVLLSPSSNA
CCCCEEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MIVRLKELLHNIVNADFDIEIKGVTCNPKRVKEGYLFVCVSERSKEHVTCLPNGCVHNCV
CEEHHHHHHHHHHCCCCEEEEEEEECCHHHCCCCEEEEEECCCCCCCEEECCCCHHHHHH
DVVIQGAKKDVIPVLRHWDPENLIVNKHTRQLYNENWIPVSATRMTSTPSFAVLYHSDPQ
HHHHCCCCCHHHHHHHCCCCCCEEEEHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCH
GIYSEIVSRFYQFKQPKYVAAVTGTNGKTSVVEFCRQIWQDNVASIGTLGTCVNNNRKDN
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
SLTTPSADDLYSTLRDINNKNVEHLALEASSHGIDQYRIHGLRLNAAAFTNFSQDHLDYH
CCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEEEEHHHHCCCCHHHHHHH
KSIDEYFEAKKRLFDEVLPKEKTAILNADIGEYGELLNIAEKRGNKVITYGKKNSDITLL
HHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
KQIPTANGQYLTIQIGDEIYNAFFPIFGQFQAYNLLCAIGIVISSGMDYQRMCIDKLASP
EECCCCCCCEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
SGRMEKVNTFAFVDYAHTPSALKQALLSLKWHFNKKIVLVFGCGGNRDQTKRAEMGKIAQ
CCCCHHEEEEEEEEECCCHHHHHHHHHHHHEECCCEEEEEEECCCCCCHHHHHHHHHHHH
MLAGKVIVTDDNPRDEDPGKIRHDILLHCPDALEIGDRKKAIEKGINIAHDENMILLVAG
HHCCEEEEECCCCCCCCCCCEECCEEEECCCHHCCCCHHHHHHHCCCEEECCCEEEEEEC
KGHERFQIIEGQSLEFSDVEVIREGQKFATAVLLSPSSNA
CCCCEEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA