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The map label for this gene is gatB [H]

Identifier: 190570557

GI number: 190570557

Start: 84405

End: 85826

Strand: Direct

Name: gatB [H]

Synonym: WPa_0087

Alternate gene names: 190570557

Gene position: 84405-85826 (Clockwise)

Preceding gene: 190570556

Following gene: 190570562

Centisome position: 5.69

GC content: 36.08

Gene sequence:

>1422_bases
ATGACAAAAGAAAACTGGGAGACAGTAATTGGACTTGAGGTACACGCTCAGGTTTCTTCTAAGACAAAGCTATTTTCTAG
TTCACTAACGGAATTTGGCACTGAGCACAATACTCAAGTTTCTCTAGTTGATGCAGCAATGCCAGGTACATTGCCAATAT
TAAATTATTTCTGCATAGAGCAAGCAATATGTACCGGTCTTGCAATTTCTGCAGAAATTAATAAATGCTCTTACTTTGAC
CGGAAAAATTATTTTTATCCCGATTTACCACAAGGTTATCAAATAACCCAGTTCTTTGAGCCAATAGTTAAAAATGGTAA
AGTGTTTATCAATAATAATGAAAAAGAAATAAGAATCGCAAGAATTCACTTAGAGCAAGATGCAGGAAAGAGTATTCATG
AAGAAAGCAAAACTTACGTAGATTTAAATCGTGCAGGGGTTGCTTTAATGGAAATTGTTTCAGAGCCAGATCTTCGTTCA
TCTGCAGAAGCTGCAGAATTCATGAAAAAATTGAGGCAGATTTTGCGTTATATCGGTTCATGTGATGGTGATATGGAAAA
GGGGTCGCTTCGCTGTGATGCAAATGTTTCTGTTCGCCCAAATGGTAGTAGTGCATTTGGCACCCGCTGTGAAATAAAAA
ACTTAAATTCAATACGTTATATTGTACAAGCTATAGATTATGAAGCACAAAGGCAGATCAAAATTTTGGAAAGCGGAGGA
GAAATAAGTCAAGATACCTTATTGTTTGATGTCACTTTAGGAAAAACAAAAGTGATGAGAAGCAAAGAAGATTCAAGTGA
CTATAGATATTTCCCTGAACCTGATTTGCTACCTGTTGAAATAAGCCAAGACAAAATTGATTCTATTAAATCATCTTTAC
CCGAGTTACCAGATCAAAAAAAACTGCGGTACATTAATGAATTAGGTATAAATGAATACGATGCAGACGTTATCACTTCT
GATAAAGCAACCGCTGATTACTTTGAGGAATTAATAAAAAAGCATGATTCAAAGATTGCAGTTACCTGGTTAACCGTAGA
GCTTTTCGGTCGTTTAAACAAAGCAAATATTGATATTGTTAGCTCCCCAATTAAAGCAGATGCTTTGTCCGAGCTCCTCG
ACTTTATCGTCGATGGAACGATCTCTGCTAAACTTGGCAAACAAGTTTTTGATATTATGTTTGAAACTGGCAAACCTGCG
TCTTTGATTATAGAAGAACAGGACCTCAAGCAAATAACCGATAGAGATCAAATATCGGAAATTATCGACACAATCGTCAA
TAACAACCAAGATAAAGTTCAAGAATACAAAAGCGGTAAAACAAAACTATACGGATTCTTTGTTGGCGAAGTTATGAAAT
CCACTAAGGGAAAAGCCAGCCCTGATGTCGTAAACTTGGTTTTGAGTGAAAGATTAGGGTAA

Upstream 100 bases:

>100_bases
ATTATTGATATACACCGCTCTTGGATCATAGGTTAAATGAGTTTTATTTGACTTTTTGTGCTAGTTTGCAGAATATAAAG
TTATTAATTGAATAAAATAC

Downstream 100 bases:

>100_bases
AAACAGGGGTAATGTATCTAGAAGATGATTCTAATTATGGGGGGTTCAAACGCCCTTACCCTGGTATTACCTTCCAAGAG
TTATTGATCGTAATATCTCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTKENWETVIGLEVHAQVSSKTKLFSSSLTEFGTEHNTQVSLVDAAMPGTLPILNYFCIEQAICTGLAISAEINKCSYFD
RKNYFYPDLPQGYQITQFFEPIVKNGKVFINNNEKEIRIARIHLEQDAGKSIHEESKTYVDLNRAGVALMEIVSEPDLRS
SAEAAEFMKKLRQILRYIGSCDGDMEKGSLRCDANVSVRPNGSSAFGTRCEIKNLNSIRYIVQAIDYEAQRQIKILESGG
EISQDTLLFDVTLGKTKVMRSKEDSSDYRYFPEPDLLPVEISQDKIDSIKSSLPELPDQKKLRYINELGINEYDADVITS
DKATADYFEELIKKHDSKIAVTWLTVELFGRLNKANIDIVSSPIKADALSELLDFIVDGTISAKLGKQVFDIMFETGKPA
SLIIEEQDLKQITDRDQISEIIDTIVNNNQDKVQEYKSGKTKLYGFFVGEVMKSTKGKASPDVVNLVLSERLG

Sequences:

>Translated_473_residues
MTKENWETVIGLEVHAQVSSKTKLFSSSLTEFGTEHNTQVSLVDAAMPGTLPILNYFCIEQAICTGLAISAEINKCSYFD
RKNYFYPDLPQGYQITQFFEPIVKNGKVFINNNEKEIRIARIHLEQDAGKSIHEESKTYVDLNRAGVALMEIVSEPDLRS
SAEAAEFMKKLRQILRYIGSCDGDMEKGSLRCDANVSVRPNGSSAFGTRCEIKNLNSIRYIVQAIDYEAQRQIKILESGG
EISQDTLLFDVTLGKTKVMRSKEDSSDYRYFPEPDLLPVEISQDKIDSIKSSLPELPDQKKLRYINELGINEYDADVITS
DKATADYFEELIKKHDSKIAVTWLTVELFGRLNKANIDIVSSPIKADALSELLDFIVDGTISAKLGKQVFDIMFETGKPA
SLIIEEQDLKQITDRDQISEIIDTIVNNNQDKVQEYKSGKTKLYGFFVGEVMKSTKGKASPDVVNLVLSERLG
>Mature_472_residues
TKENWETVIGLEVHAQVSSKTKLFSSSLTEFGTEHNTQVSLVDAAMPGTLPILNYFCIEQAICTGLAISAEINKCSYFDR
KNYFYPDLPQGYQITQFFEPIVKNGKVFINNNEKEIRIARIHLEQDAGKSIHEESKTYVDLNRAGVALMEIVSEPDLRSS
AEAAEFMKKLRQILRYIGSCDGDMEKGSLRCDANVSVRPNGSSAFGTRCEIKNLNSIRYIVQAIDYEAQRQIKILESGGE
ISQDTLLFDVTLGKTKVMRSKEDSSDYRYFPEPDLLPVEISQDKIDSIKSSLPELPDQKKLRYINELGINEYDADVITSD
KATADYFEELIKKHDSKIAVTWLTVELFGRLNKANIDIVSSPIKADALSELLDFIVDGTISAKLGKQVFDIMFETGKPAS
LIIEEQDLKQITDRDQISEIIDTIVNNNQDKVQEYKSGKTKLYGFFVGEVMKSTKGKASPDVVNLVLSERLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=39.0688259109312, Blast_Score=344, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI115534094, Length=305, Percent_Identity=42.2950819672131, Blast_Score=224, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=505, Percent_Identity=31.6831683168317, Blast_Score=229, Evalue=8e-61,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=37.1134020618557, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: NA

Molecular weight: Translated: 53133; Mature: 53002

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKENWETVIGLEVHAQVSSKTKLFSSSLTEFGTEHNTQVSLVDAAMPGTLPILNYFCIE
CCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
QAICTGLAISAEINKCSYFDRKNYFYPDLPQGYQITQFFEPIVKNGKVFINNNEKEIRIA
HHHHCCEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEE
RIHLEQDAGKSIHEESKTYVDLNRAGVALMEIVSEPDLRSSAEAAEFMKKLRQILRYIGS
EEEECCCCCCHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
CDGDMEKGSLRCDANVSVRPNGSSAFGTRCEIKNLNSIRYIVQAIDYEAQRQIKILESGG
CCCCCCCCCEEECCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHCHHHHHHEEEEECCC
EISQDTLLFDVTLGKTKVMRSKEDSSDYRYFPEPDLLPVEISQDKIDSIKSSLPELPDQK
CCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCHH
KLRYINELGINEYDADVITSDKATADYFEELIKKHDSKIAVTWLTVELFGRLNKANIDIV
HHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHCCCCCCEEEE
SSPIKADALSELLDFIVDGTISAKLGKQVFDIMFETGKPASLIIEEQDLKQITDRDQISE
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCHHHHHH
IIDTIVNNNQDKVQEYKSGKTKLYGFFVGEVMKSTKGKASPDVVNLVLSERLG
HHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKENWETVIGLEVHAQVSSKTKLFSSSLTEFGTEHNTQVSLVDAAMPGTLPILNYFCIE
CCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
QAICTGLAISAEINKCSYFDRKNYFYPDLPQGYQITQFFEPIVKNGKVFINNNEKEIRIA
HHHHCCEEEEEECCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEE
RIHLEQDAGKSIHEESKTYVDLNRAGVALMEIVSEPDLRSSAEAAEFMKKLRQILRYIGS
EEEECCCCCCHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
CDGDMEKGSLRCDANVSVRPNGSSAFGTRCEIKNLNSIRYIVQAIDYEAQRQIKILESGG
CCCCCCCCCEEECCCEEECCCCCCCCCCEEEECCCHHHHHHHHHHCHHHHHHEEEEECCC
EISQDTLLFDVTLGKTKVMRSKEDSSDYRYFPEPDLLPVEISQDKIDSIKSSLPELPDQK
CCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCHH
KLRYINELGINEYDADVITSDKATADYFEELIKKHDSKIAVTWLTVELFGRLNKANIDIV
HHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHCCCCCCEEEE
SSPIKADALSELLDFIVDGTISAKLGKQVFDIMFETGKPASLIIEEQDLKQITDRDQISE
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCHHHHHH
IIDTIVNNNQDKVQEYKSGKTKLYGFFVGEVMKSTKGKASPDVVNLVLSERLG
HHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA