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The map label for this gene is cysS

Identifier: 190570547

GI number: 190570547

Start: 71937

End: 73331

Strand: Direct

Name: cysS

Synonym: WPa_0077

Alternate gene names: 190570547

Gene position: 71937-73331 (Clockwise)

Preceding gene: 190570546

Following gene: 190570548

Centisome position: 4.85

GC content: 34.19

Gene sequence:

>1395_bases
ATGGTTAAGCTTTACAACACTTTAACAAAAAAAAAAGAGCCCTTTACACCGATCGATAAAGATCATATAAAAATGTACGT
TTGTGGGCCAACAGTATATGATACAGCACATATAGGTAATGCACGTTCTATTGTTGTTTACGATGTATTATTTCGACTGC
TTAAATTTTGTTATGGAAAAGTTACTTATGTGCGTAATATAACCGACATCGATGACAAGATAATCAATGCAGCAAATGAG
AAAAATAGTAATATAGAAAGCATTAGCAAATATTACACTAAAACTTTTCATGAAGACATGGAAAGCATAAATTGTGCAGA
ACCAACATATGAACCAAAAGCAACGGAAAACATAGATAACATTATCAAGTTAATTGAGTCTTTGCTGCAAGCTGGTCATG
CTTATGAATCCAATAAACATGTATATTTTAGTGTAGAATCTTACCCTGAATACGGCGTTTTATCAGGAAAAAAAATTGAT
GAATTAAACTACGGTAGTAGAGTTGAAGTTGGTGAAAATAAAAAACACCCAGGAGACTTCGTACTTTGGAAACCTGCAAA
CGAAACTGACTATAAACTTTCAAGTCACTGGAATAGTCCATGGGGAGAGGGAAGACCAGGGTGGCATATAGAATGTTCAG
CAATGTCATATGCTTACCTTGGCAAAGATTTTGACATTCACGGTGGGGGTATAGACTTGCAATTTCCTCATCATGAGAAT
GAAATTGCACAGAGTAAATCTGCATTTGCTGAGTCAACGTTTGCAAAATATTGGGTACACAACGGGTTTCTTACGGTAAA
TGAAGAGAAAATGAGCAAATCCTTATTTAATATAGTCAAAGTAAGGGATTTGTTAGATAGTGGAATAAAGGGTGAAGTAA
TACGTTATGCGCTGCTTAAAACTCACTACAGAAAACCACTTGATTGGACAGAAAATGTTATCTCTGAGTCGCAAGAAACT
TTAAACAAGTTTTATCGGTTATTACGTAGTACATGTATTGAGGAAAGTGATACAGAAGTCTCTAAAGATTTTATAGAGGC
TTTGAAAAACGATTTAAACATTCCTGAAGCTGTAGCCATATTGCATGAAATGGCTACAGAAATCAATAAGACAAGTAATG
AATATGAGAAGCTCAAATTAACTAAGAAGTTTGTTAAAAGCGCAAGGTTCATTGGTATTCTTGAGTCAAGTTATCAAGAG
TGGTTTGCAAGTGGTGTGAGTCATCAAGAAATAGAAAGGTTGATAGACTTAAGAAAAGTAGCAAAACAAAATAAAGATTA
TGATACTGCAGACAAAATAAGAGAGCAATTGAAACAAATGGGGGTTACAATTTCTGACAACGAAGATGGTACAACAACTT
GGTATGGAAAATTATCTCCTATTGTTTATAGATAG

Upstream 100 bases:

>100_bases
GTATTAGTATCATTGTGTGGGTTATTACTTACTCTTGTCGTAATAGTTAAAGGACCACAGCTTATATTTGGAGCATTTTC
TTTTCTAAAAAGCTTTGTAT

Downstream 100 bases:

>100_bases
CCTAGTATCATCGCTTATCTTACCCATACATCAAGGTTTTGTACTTTACACTATGTTATGCTTTAGGTCAAACTACGATC
AACTACTTATATTACTTTTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MVKLYNTLTKKKEPFTPIDKDHIKMYVCGPTVYDTAHIGNARSIVVYDVLFRLLKFCYGKVTYVRNITDIDDKIINAANE
KNSNIESISKYYTKTFHEDMESINCAEPTYEPKATENIDNIIKLIESLLQAGHAYESNKHVYFSVESYPEYGVLSGKKID
ELNYGSRVEVGENKKHPGDFVLWKPANETDYKLSSHWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EIAQSKSAFAESTFAKYWVHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLKTHYRKPLDWTENVISESQET
LNKFYRLLRSTCIEESDTEVSKDFIEALKNDLNIPEAVAILHEMATEINKTSNEYEKLKLTKKFVKSARFIGILESSYQE
WFASGVSHQEIERLIDLRKVAKQNKDYDTADKIREQLKQMGVTISDNEDGTTTWYGKLSPIVYR

Sequences:

>Translated_464_residues
MVKLYNTLTKKKEPFTPIDKDHIKMYVCGPTVYDTAHIGNARSIVVYDVLFRLLKFCYGKVTYVRNITDIDDKIINAANE
KNSNIESISKYYTKTFHEDMESINCAEPTYEPKATENIDNIIKLIESLLQAGHAYESNKHVYFSVESYPEYGVLSGKKID
ELNYGSRVEVGENKKHPGDFVLWKPANETDYKLSSHWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EIAQSKSAFAESTFAKYWVHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLKTHYRKPLDWTENVISESQET
LNKFYRLLRSTCIEESDTEVSKDFIEALKNDLNIPEAVAILHEMATEINKTSNEYEKLKLTKKFVKSARFIGILESSYQE
WFASGVSHQEIERLIDLRKVAKQNKDYDTADKIREQLKQMGVTISDNEDGTTTWYGKLSPIVYR
>Mature_464_residues
MVKLYNTLTKKKEPFTPIDKDHIKMYVCGPTVYDTAHIGNARSIVVYDVLFRLLKFCYGKVTYVRNITDIDDKIINAANE
KNSNIESISKYYTKTFHEDMESINCAEPTYEPKATENIDNIIKLIESLLQAGHAYESNKHVYFSVESYPEYGVLSGKKID
ELNYGSRVEVGENKKHPGDFVLWKPANETDYKLSSHWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EIAQSKSAFAESTFAKYWVHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLKTHYRKPLDWTENVISESQET
LNKFYRLLRSTCIEESDTEVSKDFIEALKNDLNIPEAVAILHEMATEINKTSNEYEKLKLTKKFVKSARFIGILESSYQE
WFASGVSHQEIERLIDLRKVAKQNKDYDTADKIREQLKQMGVTISDNEDGTTTWYGKLSPIVYR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=515, Percent_Identity=32.0388349514563, Blast_Score=249, Evalue=6e-66,
Organism=Homo sapiens, GI303304987, Length=275, Percent_Identity=33.0909090909091, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI62240992, Length=275, Percent_Identity=33.0909090909091, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI21269877, Length=275, Percent_Identity=33.0909090909091, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI10835051, Length=275, Percent_Identity=33.0909090909091, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1786737, Length=463, Percent_Identity=43.6285097192225, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17509795, Length=270, Percent_Identity=31.1111111111111, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17509797, Length=270, Percent_Identity=31.1111111111111, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324082, Length=316, Percent_Identity=32.2784810126582, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI19922218, Length=397, Percent_Identity=32.9974811083123, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI19922364, Length=275, Percent_Identity=36, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_WOLPP (B3CN54)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001974905.1
- EnsemblBacteria:   EBWOLT00000000409
- GeneID:   6384282
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0077
- GeneTree:   EBGT00050000031245
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53429; Mature: 53429

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLYNTLTKKKEPFTPIDKDHIKMYVCGPTVYDTAHIGNARSIVVYDVLFRLLKFCYGK
CCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCH
VTYVRNITDIDDKIINAANEKNSNIESISKYYTKTFHEDMESINCAEPTYEPKATENIDN
HHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHH
IIKLIESLLQAGHAYESNKHVYFSVESYPEYGVLSGKKIDELNYGSRVEVGENKKHPGDF
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCE
VLWKPANETDYKLSSHWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EEECCCCCCCCCHHCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEECCCCEEEECCCCHH
EIAQSKSAFAESTFAKYWVHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLK
HHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
THYRKPLDWTENVISESQETLNKFYRLLRSTCIEESDTEVSKDFIEALKNDLNIPEAVAI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHH
LHEMATEINKTSNEYEKLKLTKKFVKSARFIGILESSYQEWFASGVSHQEIERLIDLRKV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AKQNKDYDTADKIREQLKQMGVTISDNEDGTTTWYGKLSPIVYR
HHCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCEECC
>Mature Secondary Structure
MVKLYNTLTKKKEPFTPIDKDHIKMYVCGPTVYDTAHIGNARSIVVYDVLFRLLKFCYGK
CCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCH
VTYVRNITDIDDKIINAANEKNSNIESISKYYTKTFHEDMESINCAEPTYEPKATENIDN
HHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHH
IIKLIESLLQAGHAYESNKHVYFSVESYPEYGVLSGKKIDELNYGSRVEVGENKKHPGDF
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCE
VLWKPANETDYKLSSHWNSPWGEGRPGWHIECSAMSYAYLGKDFDIHGGGIDLQFPHHEN
EEECCCCCCCCCHHCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEECCCCEEEECCCCHH
EIAQSKSAFAESTFAKYWVHNGFLTVNEEKMSKSLFNIVKVRDLLDSGIKGEVIRYALLK
HHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
THYRKPLDWTENVISESQETLNKFYRLLRSTCIEESDTEVSKDFIEALKNDLNIPEAVAI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHH
LHEMATEINKTSNEYEKLKLTKKFVKSARFIGILESSYQEWFASGVSHQEIERLIDLRKV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
AKQNKDYDTADKIREQLKQMGVTISDNEDGTTTWYGKLSPIVYR
HHCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA