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The map label for this gene is sucB [H]

Identifier: 190570534

GI number: 190570534

Start: 56736

End: 57908

Strand: Direct

Name: sucB [H]

Synonym: WPa_0062

Alternate gene names: 190570534

Gene position: 56736-57908 (Clockwise)

Preceding gene: 190570530

Following gene: 190570535

Centisome position: 3.83

GC content: 36.83

Gene sequence:

>1173_bases
ATGAGCAAAATTATAGAAATCAGAGCGCCAAAAACTCTTGGTGGTGAATCAGTTACGGAAGGTATAGTAAAAATAAAGAA
AAATATCGGCGAAGCAGTAAAAGTAGATGACTTGATCTTTGAAATTGAAACTGATAAAACGGCGCTAGAACTAACTGCAG
AAGCTTCAGGACAAATAACTGAATTTTTTGTGAAAGAAGATGATATAATTAGCCCTGATCAATTACTGGCAAAACTTGCT
GCAGGAGAAGTAGAAGAAGAAGTGAAAAAAGAAGATAAAGGCGAAGGCCCTGATAAAAAAGATGCCCCTTCAGCTCGTAA
AATTATGGAAGAAAATGCAATTAGTGCAGAAAATGTAAAAGGAACTGGCATGGGAGGTAGAATAACTAAAGCGGATGTGA
TAGACCATATGAGTAAAGCTGAACAACCTTCGGTAAAACAATATGAATCGCCAAAAAGTGTAGTAAGTGGAGAGAGAAGA
GAAGAGCGAGTGAAAATGAGCAAAATAAGGCAAGTAATAGCTGCTCGTTTGAAAGCATCGCAAAATACTGCTGCAATACT
GACCACGTTCAATGAAATTGACATGAAAAACGTCATGGATCTGAGGGCAAAGTATAAAGAAACTTTTGAAAAGAAATATG
GAATAAAACTGGGTTTCATGTCGTTTTTTATAAAGGCAGCAGTGCAAGCACTAAAAGAAATTCGTGAGATTAACGCTGAG
ATTTCAGGTGATGAAATTGTATATAAAAATTACTATGACATAGGTGTTGCTGTTGGCACTGATAAAGGTCTTGTTGTACC
AGTTATTCGTGATGCTGATCAAATGTCATTTGCTGAAATTGAACTAACTTTAGTTGCTCTTGGCAAAAAAGCACGAGAAG
GTAAGCTGCAAGTATCGGAAATGGAAGGTGCAACATTTACTATCTCAAACGGTGGTGTATATGGTTCACTTCTTTCTACT
CCGATAATAAACCCTCCGCAATCTGGAATACTTGGCATGCACTCAATACAAAATAGGCCAGTTGCTGTGAGTAGCTCAAT
TGAAATCAGACCTATGATGTACATTTCCCTCTCTTACGACCACAGAATAGTTGATGGCAAAGGAGCAGTTACTTTCCTTG
TTAAAATCAAAAATTACATAGAAGATCCAAATAGATTGGTTTTGGAAATTTAA

Upstream 100 bases:

>100_bases
AGGAGGAAAGAATCAAGCATAAGGCCTAATGAATTTATTAGTTTATTTATGTTTCTTTTCATGTATAAATTTATAGAAAG
TTTTATATAGAAGTTATTTT

Downstream 100 bases:

>100_bases
ACAGATTAAAAAAAGACAAGTAAGTTTTTCTGAAGTTTTTAAGGTGGGTGTTACAGCTGTACAAACATTTGTTTATAAAG
GTAACTTATACGAAAAATGG

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSKIIEIRAPKTLGGESVTEGIVKIKKNIGEAVKVDDLIFEIETDKTALELTAEASGQITEFFVKEDDIISPDQLLAKLA
AGEVEEEVKKEDKGEGPDKKDAPSARKIMEENAISAENVKGTGMGGRITKADVIDHMSKAEQPSVKQYESPKSVVSGERR
EERVKMSKIRQVIAARLKASQNTAAILTTFNEIDMKNVMDLRAKYKETFEKKYGIKLGFMSFFIKAAVQALKEIREINAE
ISGDEIVYKNYYDIGVAVGTDKGLVVPVIRDADQMSFAEIELTLVALGKKAREGKLQVSEMEGATFTISNGGVYGSLLST
PIINPPQSGILGMHSIQNRPVAVSSSIEIRPMMYISLSYDHRIVDGKGAVTFLVKIKNYIEDPNRLVLEI

Sequences:

>Translated_390_residues
MSKIIEIRAPKTLGGESVTEGIVKIKKNIGEAVKVDDLIFEIETDKTALELTAEASGQITEFFVKEDDIISPDQLLAKLA
AGEVEEEVKKEDKGEGPDKKDAPSARKIMEENAISAENVKGTGMGGRITKADVIDHMSKAEQPSVKQYESPKSVVSGERR
EERVKMSKIRQVIAARLKASQNTAAILTTFNEIDMKNVMDLRAKYKETFEKKYGIKLGFMSFFIKAAVQALKEIREINAE
ISGDEIVYKNYYDIGVAVGTDKGLVVPVIRDADQMSFAEIELTLVALGKKAREGKLQVSEMEGATFTISNGGVYGSLLST
PIINPPQSGILGMHSIQNRPVAVSSSIEIRPMMYISLSYDHRIVDGKGAVTFLVKIKNYIEDPNRLVLEI
>Mature_389_residues
SKIIEIRAPKTLGGESVTEGIVKIKKNIGEAVKVDDLIFEIETDKTALELTAEASGQITEFFVKEDDIISPDQLLAKLAA
GEVEEEVKKEDKGEGPDKKDAPSARKIMEENAISAENVKGTGMGGRITKADVIDHMSKAEQPSVKQYESPKSVVSGERRE
ERVKMSKIRQVIAARLKASQNTAAILTTFNEIDMKNVMDLRAKYKETFEKKYGIKLGFMSFFIKAAVQALKEIREINAEI
SGDEIVYKNYYDIGVAVGTDKGLVVPVIRDADQMSFAEIELTLVALGKKAREGKLQVSEMEGATFTISNGGVYGSLLSTP
IINPPQSGILGMHSIQNRPVAVSSSIEIRPMMYISLSYDHRIVDGKGAVTFLVKIKNYIEDPNRLVLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=55.7312252964427, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI31711992, Length=293, Percent_Identity=32.7645051194539, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI203098753, Length=439, Percent_Identity=30.0683371298405, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI203098816, Length=439, Percent_Identity=30.0683371298405, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=28.2296650717703, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.2670807453416, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786946, Length=393, Percent_Identity=45.29262086514, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1786305, Length=304, Percent_Identity=32.5657894736842, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=406, Percent_Identity=43.8423645320197, Blast_Score=318, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17537937, Length=408, Percent_Identity=29.656862745098, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560088, Length=423, Percent_Identity=28.6052009456265, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=234, Percent_Identity=32.4786324786325, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=386, Percent_Identity=45.5958549222798, Blast_Score=315, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=440, Percent_Identity=25.6818181818182, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=57.9399141630901, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=31.2714776632302, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=34.6320346320346, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=26.8235294117647, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42907; Mature: 42776

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIIEIRAPKTLGGESVTEGIVKIKKNIGEAVKVDDLIFEIETDKTALELTAEASGQIT
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCEE
EFFVKEDDIISPDQLLAKLAAGEVEEEVKKEDKGEGPDKKDAPSARKIMEENAISAENVK
EEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCC
GTGMGGRITKADVIDHMSKAEQPSVKQYESPKSVVSGERREERVKMSKIRQVIAARLKAS
CCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
QNTAAILTTFNEIDMKNVMDLRAKYKETFEKKYGIKLGFMSFFIKAAVQALKEIREINAE
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
ISGDEIVYKNYYDIGVAVGTDKGLVVPVIRDADQMSFAEIELTLVALGKKAREGKLQVSE
CCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHEEEEEEEECCCCCCCCEEEEE
MEGATFTISNGGVYGSLLSTPIINPPQSGILGMHSIQNRPVAVSSSIEIRPMMYISLSYD
CCCCEEEEECCCEEHHHHCCCCCCCCCCCCEEHHHCCCCCEEECCCEEEEEEEEEEEECC
HRIVDGKGAVTFLVKIKNYIEDPNRLVLEI
CEEECCCCCEEEEEEHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SKIIEIRAPKTLGGESVTEGIVKIKKNIGEAVKVDDLIFEIETDKTALELTAEASGQIT
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCEE
EFFVKEDDIISPDQLLAKLAAGEVEEEVKKEDKGEGPDKKDAPSARKIMEENAISAENVK
EEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCC
GTGMGGRITKADVIDHMSKAEQPSVKQYESPKSVVSGERREERVKMSKIRQVIAARLKAS
CCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
QNTAAILTTFNEIDMKNVMDLRAKYKETFEKKYGIKLGFMSFFIKAAVQALKEIREINAE
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
ISGDEIVYKNYYDIGVAVGTDKGLVVPVIRDADQMSFAEIELTLVALGKKAREGKLQVSE
CCCCEEEEEEEEEEEEEEECCCCEEEEEECCCCCCCHHHEEEEEEEECCCCCCCCEEEEE
MEGATFTISNGGVYGSLLSTPIINPPQSGILGMHSIQNRPVAVSSSIEIRPMMYISLSYD
CCCCEEEEECCCEEHHHHCCCCCCCCCCCCEEHHHCCCCCEEECCCEEEEEEEEEEEECC
HRIVDGKGAVTFLVKIKNYIEDPNRLVLEI
CEEECCCCCEEEEEEHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA