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The map label for this gene is nrdF [H]

Identifier: 190570498

GI number: 190570498

Start: 15138

End: 16127

Strand: Direct

Name: nrdF [H]

Synonym: WPa_0020

Alternate gene names: 190570498

Gene position: 15138-16127 (Clockwise)

Preceding gene: 190570496

Following gene: 190570499

Centisome position: 1.02

GC content: 33.94

Gene sequence:

>990_bases
ATGTCATTGCTAGAAGCAGATCCAATATACAAGCCTTTTAATTATCCTTGGGCATATGATGCATGGTTGCAGCAACAAAG
GATACACTGGATACCTGAAGAAGTTCCGCTTGCTGATGATGTGAAAGATTGGAAAACTAAACTTTCGAATGTAGAGAAAA
ATTTATTGACCCAGATTTTTAGGTTTTTTACTCAAGCAGATATTGAAGTAAATAATTGCTATATGAGGCATTATTCAAAT
ATATTTAAGCCAACAGAAATATGTATGATGCTTGCAAGCTTTTCCAATATGGAGACTATACACATTGCAGCCTATTCATA
CCTTCTAGATACGATTGGCATGCCAGAAAGTGAATATCAAGCATTCTTAAAATATGATGCTATGAGAAAGAAGTATGAAT
ATATGTTAGAATTTGAGGAAAGTAAAAAGCAAGATAAAAAACATGTTGCTAAAACTTTAGCAGTATTTGGTGCCTTCACT
GAAGGATTGCAGTTATTTGCATCATTTGCAATTTTGCTCAATTTTCAACGGTTTGGAAAAATGAAAGGTATGGGGCAAAT
AATTGCTTGGTCAGCACGTGACGAAACCTTGCATACCAATTCAATTATCAATTTGTTCAATACGTTTATTAAAGAGAATA
ATGAAATTTGGAATGACGAGTTCAAAGAAGAATTATATTCTGCATGTCGCACTATTGTTGAACTTGAAGATGAATTTATA
AGTCTTGCTTTTGATTTAGGAGATGTTGAAGGGCTATCTGCAGAGGAAGTGCGCAATTATATACGTTACATAGCAAACAG
AAGGTTAACGCAATTAGGTCTTAAATCTATATATAATGTTAAAGATAACCCTATTCCGTGGCTTGATGAAATACTAAATG
GCGTGGAACATACAAATTTCTTTGAAAATAGAGTAACAGAGTATAGCCGTGCAGCAACTCAAGGCACTTGGGAGGAAGCT
TTTGCTAAAAACTATACAAAAAGTGAATAG

Upstream 100 bases:

>100_bases
ACACTGATAAACTATCTGTCGTCAAATTTCAAAAAAGTTAAATTGACTGATTCTCAATATCATTTAATATAATATTTACT
TCAAAATAATTAGGTACCTT

Downstream 100 bases:

>100_bases
CAGTAACTTCGGTTCTGTCGCATTTGTAGGGAAGTAGAAAAAAGAATGATATAATTGTCTAAAAATAAAGCAAAGATGTT
TCGTATCAATAAAAAATTAT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSNVEKNLLTQIFRFFTQADIEVNNCYMRHYSN
IFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQAFLKYDAMRKKYEYMLEFEESKKQDKKHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSARDETLHTNSIINLFNTFIKENNEIWNDEFKEELYSACRTIVELEDEFI
SLAFDLGDVEGLSAEEVRNYIRYIANRRLTQLGLKSIYNVKDNPIPWLDEILNGVEHTNFFENRVTEYSRAATQGTWEEA
FAKNYTKSE

Sequences:

>Translated_329_residues
MSLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSNVEKNLLTQIFRFFTQADIEVNNCYMRHYSN
IFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQAFLKYDAMRKKYEYMLEFEESKKQDKKHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSARDETLHTNSIINLFNTFIKENNEIWNDEFKEELYSACRTIVELEDEFI
SLAFDLGDVEGLSAEEVRNYIRYIANRRLTQLGLKSIYNVKDNPIPWLDEILNGVEHTNFFENRVTEYSRAATQGTWEEA
FAKNYTKSE
>Mature_328_residues
SLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSNVEKNLLTQIFRFFTQADIEVNNCYMRHYSNI
FKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQAFLKYDAMRKKYEYMLEFEESKKQDKKHVAKTLAVFGAFTE
GLQLFASFAILLNFQRFGKMKGMGQIIAWSARDETLHTNSIINLFNTFIKENNEIWNDEFKEELYSACRTIVELEDEFIS
LAFDLGDVEGLSAEEVRNYIRYIANRRLTQLGLKSIYNVKDNPIPWLDEILNGVEHTNFFENRVTEYSRAATQGTWEEAF
AKNYTKSE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=309, Percent_Identity=28.4789644012945, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI289177074, Length=309, Percent_Identity=28.8025889967638, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI4557845, Length=313, Percent_Identity=26.8370607028754, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260064013, Length=313, Percent_Identity=26.8370607028754, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI289177076, Length=288, Percent_Identity=28.125, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1788567, Length=299, Percent_Identity=24.4147157190635, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789031, Length=287, Percent_Identity=24.7386759581882, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17551968, Length=289, Percent_Identity=28.719723183391, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322434, Length=318, Percent_Identity=27.6729559748428, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18079279, Length=319, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38730; Mature: 38599

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSNVEKNLLTQIF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVNNCYMRHYSNIFKPTEICMMLASFSNMETIHIAAYSYLLDTIGMPESEYQ
HHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHH
AFLKYDAMRKKYEYMLEFEESKKQDKKHVAKTLAVFGAFTEGLQLFASFAILLNFQRFGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGMGQIIAWSARDETLHTNSIINLFNTFIKENNEIWNDEFKEELYSACRTIVELEDEFI
HCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SLAFDLGDVEGLSAEEVRNYIRYIANRRLTQLGLKSIYNVKDNPIPWLDEILNGVEHTNF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
FENRVTEYSRAATQGTWEEAFAKNYTKSE
HHHHHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLLEADPIYKPFNYPWAYDAWLQQQRIHWIPEEVPLADDVKDWKTKLSNVEKNLLTQIF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]