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The map label for this gene is ansA [H]

Identifier: 18978419

GI number: 18978419

Start: 1888256

End: 1889236

Strand: Direct

Name: ansA [H]

Synonym: PF2047

Alternate gene names: 18978419

Gene position: 1888256-1889236 (Clockwise)

Preceding gene: 18978418

Following gene: 18978420

Centisome position: 98.95

GC content: 40.06

Gene sequence:

>981_bases
GTGAAAATTCTTCTAATTGGGATGGGTGGAACAATTGCGAGTGTAAAGGGCGAGAATGGATATGAGGCTTCGTTGTCCGT
TAAAGAAGTTTTAGATATCGCCGGAATCAAAGATTGTGAGGATTGTGATTTTCTCGATTTAAAGAACGTTGATAGCACGC
TTATCCAGCCAGAAGATTGGGTAGATCTTGCTGAAACTCTTTACAAGAATGTAAAAAAATATGATGGAATTATAGTCACT
CATGGTACCGATACTCTTGCCTACACTTCTTCAATGATAAGTTTCATGCTTAGAAACCCCCCAATACCCATCGTATTTAC
TGGTTCTATGATACCTGCCACTGAAGAAAATAGTGATGCCCCCCTAAACTTGCAAACAGCAATAAAGTTTGCAACTTCTG
GAATTAGGGGAGTTTACGTGGCCTTCAATGGAAAAGTTATGCTTGGAGTTAGAACATCTAAGGTTAGGACAATGAGCAGA
GATGCATTCGAAAGCATTAACTACCCTATAATTGCAGAATTAAGAGGAGAAGATCTCGTGGTTAACTTTATTCCAAAGTT
TAACAATGGAGAAGTCACATTAGACCTTAGGCACGATCCAAAAGTTCTAGTTATAAAGCTAATCCCAGGACTTTCGGGGG
ACATATTTAGGGCAGCTGTAGAGCTGGGATATAGAGGAATTGTCATAGAAGGTTATGGAGCTGGAGGAATTCCTTATAGG
GGAAGTGATTTACTTCAAACAATAGAGGAGCTCTCCAAGGAGATTCCAATAGTAATGACAACCCAGGCAATGTACGATGG
AGTTGATCTAACGAGGTACAAAGTTGGGAGATTAGCCCTTAGAGCTGGAGTAATCCCAGCGGGGGACATGACAAAAGAGG
CAACAGTAACAAAGCTCATGTGGATTCTAGGCCACACAAACAATGTGGAAGAAATAAAAGTATTAATGAGAAAAAATCTA
GTTGGAGAGCTTAGAGATTAA

Upstream 100 bases:

>100_bases
TCAAAACGCTGAACAAATGTCAATTTTTCTTTTCTACACCTTATAAATTGTCGACAAAAATACTAAAAAGGGGGTATTTT
AGTTATCATGGTGATTAAAT

Downstream 100 bases:

>100_bases
CCTTCCAGCTTCTTAATTTCTTCTTCTCCAATGATTAATGGCCTAGTAGCTTCATAGACATCTGCAAGCTCCCATTTGAC
TTCTCCTTTATTCATGAGCT

Product: l-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKILLIGMGGTIASVKGENGYEASLSVKEVLDIAGIKDCEDCDFLDLKNVDSTLIQPEDWVDLAETLYKNVKKYDGIIVT
HGTDTLAYTSSMISFMLRNPPIPIVFTGSMIPATEENSDAPLNLQTAIKFATSGIRGVYVAFNGKVMLGVRTSKVRTMSR
DAFESINYPIIAELRGEDLVVNFIPKFNNGEVTLDLRHDPKVLVIKLIPGLSGDIFRAAVELGYRGIVIEGYGAGGIPYR
GSDLLQTIEELSKEIPIVMTTQAMYDGVDLTRYKVGRLALRAGVIPAGDMTKEATVTKLMWILGHTNNVEEIKVLMRKNL
VGELRD

Sequences:

>Translated_326_residues
MKILLIGMGGTIASVKGENGYEASLSVKEVLDIAGIKDCEDCDFLDLKNVDSTLIQPEDWVDLAETLYKNVKKYDGIIVT
HGTDTLAYTSSMISFMLRNPPIPIVFTGSMIPATEENSDAPLNLQTAIKFATSGIRGVYVAFNGKVMLGVRTSKVRTMSR
DAFESINYPIIAELRGEDLVVNFIPKFNNGEVTLDLRHDPKVLVIKLIPGLSGDIFRAAVELGYRGIVIEGYGAGGIPYR
GSDLLQTIEELSKEIPIVMTTQAMYDGVDLTRYKVGRLALRAGVIPAGDMTKEATVTKLMWILGHTNNVEEIKVLMRKNL
VGELRD
>Mature_326_residues
MKILLIGMGGTIASVKGENGYEASLSVKEVLDIAGIKDCEDCDFLDLKNVDSTLIQPEDWVDLAETLYKNVKKYDGIIVT
HGTDTLAYTSSMISFMLRNPPIPIVFTGSMIPATEENSDAPLNLQTAIKFATSGIRGVYVAFNGKVMLGVRTSKVRTMSR
DAFESINYPIIAELRGEDLVVNFIPKFNNGEVTLDLRHDPKVLVIKLIPGLSGDIFRAAVELGYRGIVIEGYGAGGIPYR
GSDLLQTIEELSKEIPIVMTTQAMYDGVDLTRYKVGRLALRAGVIPAGDMTKEATVTKLMWILGHTNNVEEIKVLMRKNL
VGELRD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=279, Percent_Identity=31.1827956989247, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1788065, Length=326, Percent_Identity=34.0490797546012, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789327, Length=301, Percent_Identity=26.578073089701, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=281, Percent_Identity=33.4519572953737, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI193207374, Length=281, Percent_Identity=33.4519572953737, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17558280, Length=281, Percent_Identity=33.4519572953737, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI193207372, Length=281, Percent_Identity=33.4519572953737, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6320527, Length=326, Percent_Identity=29.1411042944785, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323189, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323187, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323186, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323184, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24639609, Length=280, Percent_Identity=32.8571428571429, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24645530, Length=358, Percent_Identity=27.6536312849162, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35765; Mature: 35765

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLIGMGGTIASVKGENGYEASLSVKEVLDIAGIKDCEDCDFLDLKNVDSTLIQPEDW
CEEEEEECCCEEEEECCCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCHHH
VDLAETLYKNVKKYDGIIVTHGTDTLAYTSSMISFMLRNPPIPIVFTGSMIPATEENSDA
HHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCC
PLNLQTAIKFATSGIRGVYVAFNGKVMLGVRTSKVRTMSRDAFESINYPIIAELRGEDLV
CEEHHHHHHHHHCCCCEEEEEECCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCEEE
VNFIPKFNNGEVTLDLRHDPKVLVIKLIPGLSGDIFRAAVELGYRGIVIEGYGAGGIPYR
EEEECCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCC
GSDLLQTIEELSKEIPIVMTTQAMYDGVDLTRYKVGRLALRAGVIPAGDMTKEATVTKLM
CHHHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
WILGHTNNVEEIKVLMRKNLVGELRD
HHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKILLIGMGGTIASVKGENGYEASLSVKEVLDIAGIKDCEDCDFLDLKNVDSTLIQPEDW
CEEEEEECCCEEEEECCCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCHHH
VDLAETLYKNVKKYDGIIVTHGTDTLAYTSSMISFMLRNPPIPIVFTGSMIPATEENSDA
HHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCC
PLNLQTAIKFATSGIRGVYVAFNGKVMLGVRTSKVRTMSRDAFESINYPIIAELRGEDLV
CEEHHHHHHHHHCCCCEEEEEECCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCEEE
VNFIPKFNNGEVTLDLRHDPKVLVIKLIPGLSGDIFRAAVELGYRGIVIEGYGAGGIPYR
EEEECCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCC
GSDLLQTIEELSKEIPIVMTTQAMYDGVDLTRYKVGRLALRAGVIPAGDMTKEATVTKLM
CHHHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
WILGHTNNVEEIKVLMRKNLVGELRD
HHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]