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The map label for this gene is nplT [H]

Identifier: 18978311

GI number: 18978311

Start: 1790387

End: 1792324

Strand: Reverse

Name: nplT [H]

Synonym: PF1939

Alternate gene names: 18978311

Gene position: 1792324-1790387 (Counterclockwise)

Preceding gene: 33359583

Following gene: 18978310

Centisome position: 93.92

GC content: 37.46

Gene sequence:

>1938_bases
ATGTATAAGCTCGTCAGTTTCAGAGATAGCGAGATCTTTGGAAGAGTTGCAGAGGTGGAATTTTCCCTAATAAGAGAAGG
AAGCTATGCATACCTCCTTGGTGACTTTAATGCATTTAATGAGGGAAGTTTTAGAATGGAGCAAGAAGGAAAGAATTGGA
AGATTAAAATCGCCCTTCCAGAAGGGGTATGGCACTATGCTTTTTCAATAGATGGGAAATTCGTCTTAGATCCAGATAAT
CCTGAAAGAAGAGTATATACTAGAAAGGGTTACAAATTCCACAGAGAAGTTAATGTTGCAAGAATTGTCAAAAGTGACGA
TTTGGTTTTCCATACTCCTTCTCTCCTGTATCTGTATGAAATTTTTGGGAGGGTTCACGTTCTTTTAAGGACACAAAAGG
GGGTAATAAAAGGGGCAACTTTCCTGGGGGAGAAGCATGTTCCTATGAGGAAGAAGGCTTCTGATGAGCTATTTGATTAC
TTTGAGGTTATCGTAGAAGGAGGAGATAAGAGGTTAAATTATTCATTTGAAGTATTAACAATGGAAGGAGCGAAATTTGA
GTATGGTCAATTTAAGGCGAGACCCTTCAGTATCGAATTTCCAACCTGGGTTATTGACAGGGTATTCTATCAAATAATGC
CAGACAAATTTGCTAGGTCTAGGAAAATTCAAGGAATTGCCTATCCAAAAGATAAATATTGGGGAGGAGATCTAATCGGA
ATTAAGGAAAAGATAGACCACCTTGTGAACCTTGGAATAAATGCAATATATTTAACTCCAATCTTCAGTTCCCTCACATA
TCATGGATATGACATAGTTGATTATTTCCACGTTGCAAGGAGACTTGGAGGAGACAGGGCCTTTGTGGATTTGTTATCTG
AGCTTAAAAGGTTTGATATAAAGGTTATCCTTGATGGGGTATTTCACCATACGAGCTTTTTCCACCCATATTTCCAAGAT
GTTGTAAGGAAGGGGGAAAATAGCAGCTTTAAAAACTTTTACAGGATTATTAAATTTCCAGTAGTCTCAAAAGAATTTCT
TCAAATCCTCCATTCAAAATCTTCCTGGGAAGAAAAGTACAAAAAGATAAAATCCCTTGGCTGGAATTACGAGAGCTTTT
TCTCAGTTTGGATAATGCCGAGATTAAACCACGATAATCCAAAGGTTAGAGAGTTTATCAAGAATGTGATTCTTTTCTGG
ACAAATAAGGGGGTCGATGGATTTAGAATGGATGTTGCTCATGGGGTTCCTCCAGAAGTGTGGAAAGAAGTTAGAGAAGC
ATTGCCAAAAGAAAAGTACTTAATTGGAGAAGTTATGGATGATGCAAGGTTATGGCTGTTTGATAAATTCCATGGAGTCA
TGAACTATCGGCTCTATGATGCAATCTTAAGGTTTTTTGGATATGAGGAGATTACAGCCGAGGAATTCTTAAATGAACTA
GAGCTCTTAAGCTCGTATTATGGCCCAGCAGAGTATCTAATGTATAATTTTTTAGACAATCATGATGTTGAGAGATTTTT
GGATATAGTTGGGGATAAGAGGAAGTACGTGTGTGCTTTGGTCTTCTTGATGACTTACAAAGGTATTCCATCTCTCTTCT
ACGGAGATGAAATAGGGTTGAGAGGGATTAATCTCCAAGGAATGGAATCTTCCAGAGCACCAATGTTGTGGAATGAAGAA
GAGTGGGATCAAAGGATTTTGGAGATAACAAAGACACTAGTAAAGATTAGGAAAAACAATAAAGCCCTCCTTTTTGGTAA
TTTTGTCCCGGTGAAGTTTAAAAGAAAATTCATGGTATACAAGAGGGAACACATGGGAGAGAGAACTATCGTTGCCATTA
ACTACTCAAATTCACGCGTTAAAGAACTAGGAATAACAATTCCTGAATATTCGGGCGTGATAATTAATGAAGATAAAGTG
AAATTGATAAAGTACTAA

Upstream 100 bases:

>100_bases
TGTATTAAAGACCCCAGAAATCCACTAAGGAGATCCACTTTGTATATATTCTAACTTAACTACTGAAACGTTTTTATTCC
TTTAAGCAATTGCTACAAAC

Downstream 100 bases:

>100_bases
AATTTCTCTTTGACGTTCATTTATGTACATTAGCACAAGATATATATAGCCTTAAAATATGTATCACTATCGATGATACT
AACATGGGAGGTGTACATAT

Product: neopullulanase (alpha-amylase II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MYKLVSFRDSEIFGRVAEVEFSLIREGSYAYLLGDFNAFNEGSFRMEQEGKNWKIKIALPEGVWHYAFSIDGKFVLDPDN
PERRVYTRKGYKFHREVNVARIVKSDDLVFHTPSLLYLYEIFGRVHVLLRTQKGVIKGATFLGEKHVPMRKKASDELFDY
FEVIVEGGDKRLNYSFEVLTMEGAKFEYGQFKARPFSIEFPTWVIDRVFYQIMPDKFARSRKIQGIAYPKDKYWGGDLIG
IKEKIDHLVNLGINAIYLTPIFSSLTYHGYDIVDYFHVARRLGGDRAFVDLLSELKRFDIKVILDGVFHHTSFFHPYFQD
VVRKGENSSFKNFYRIIKFPVVSKEFLQILHSKSSWEEKYKKIKSLGWNYESFFSVWIMPRLNHDNPKVREFIKNVILFW
TNKGVDGFRMDVAHGVPPEVWKEVREALPKEKYLIGEVMDDARLWLFDKFHGVMNYRLYDAILRFFGYEEITAEEFLNEL
ELLSSYYGPAEYLMYNFLDNHDVERFLDIVGDKRKYVCALVFLMTYKGIPSLFYGDEIGLRGINLQGMESSRAPMLWNEE
EWDQRILEITKTLVKIRKNNKALLFGNFVPVKFKRKFMVYKREHMGERTIVAINYSNSRVKELGITIPEYSGVIINEDKV
KLIKY

Sequences:

>Translated_645_residues
MYKLVSFRDSEIFGRVAEVEFSLIREGSYAYLLGDFNAFNEGSFRMEQEGKNWKIKIALPEGVWHYAFSIDGKFVLDPDN
PERRVYTRKGYKFHREVNVARIVKSDDLVFHTPSLLYLYEIFGRVHVLLRTQKGVIKGATFLGEKHVPMRKKASDELFDY
FEVIVEGGDKRLNYSFEVLTMEGAKFEYGQFKARPFSIEFPTWVIDRVFYQIMPDKFARSRKIQGIAYPKDKYWGGDLIG
IKEKIDHLVNLGINAIYLTPIFSSLTYHGYDIVDYFHVARRLGGDRAFVDLLSELKRFDIKVILDGVFHHTSFFHPYFQD
VVRKGENSSFKNFYRIIKFPVVSKEFLQILHSKSSWEEKYKKIKSLGWNYESFFSVWIMPRLNHDNPKVREFIKNVILFW
TNKGVDGFRMDVAHGVPPEVWKEVREALPKEKYLIGEVMDDARLWLFDKFHGVMNYRLYDAILRFFGYEEITAEEFLNEL
ELLSSYYGPAEYLMYNFLDNHDVERFLDIVGDKRKYVCALVFLMTYKGIPSLFYGDEIGLRGINLQGMESSRAPMLWNEE
EWDQRILEITKTLVKIRKNNKALLFGNFVPVKFKRKFMVYKREHMGERTIVAINYSNSRVKELGITIPEYSGVIINEDKV
KLIKY
>Mature_645_residues
MYKLVSFRDSEIFGRVAEVEFSLIREGSYAYLLGDFNAFNEGSFRMEQEGKNWKIKIALPEGVWHYAFSIDGKFVLDPDN
PERRVYTRKGYKFHREVNVARIVKSDDLVFHTPSLLYLYEIFGRVHVLLRTQKGVIKGATFLGEKHVPMRKKASDELFDY
FEVIVEGGDKRLNYSFEVLTMEGAKFEYGQFKARPFSIEFPTWVIDRVFYQIMPDKFARSRKIQGIAYPKDKYWGGDLIG
IKEKIDHLVNLGINAIYLTPIFSSLTYHGYDIVDYFHVARRLGGDRAFVDLLSELKRFDIKVILDGVFHHTSFFHPYFQD
VVRKGENSSFKNFYRIIKFPVVSKEFLQILHSKSSWEEKYKKIKSLGWNYESFFSVWIMPRLNHDNPKVREFIKNVILFW
TNKGVDGFRMDVAHGVPPEVWKEVREALPKEKYLIGEVMDDARLWLFDKFHGVMNYRLYDAILRFFGYEEITAEEFLNEL
ELLSSYYGPAEYLMYNFLDNHDVERFLDIVGDKRKYVCALVFLMTYKGIPSLFYGDEIGLRGINLQGMESSRAPMLWNEE
EWDQRILEITKTLVKIRKNNKALLFGNFVPVKFKRKFMVYKREHMGERTIVAINYSNSRVKELGITIPEYSGVIINEDKV
KLIKY

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=421, Percent_Identity=23.5154394299287, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1786604, Length=544, Percent_Identity=27.0220588235294, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1790687, Length=530, Percent_Identity=23.3962264150943, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322245, Length=236, Percent_Identity=27.9661016949153, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586593, Length=222, Percent_Identity=34.2342342342342, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24586589, Length=391, Percent_Identity=26.8542199488491, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330053, Length=413, Percent_Identity=26.1501210653753, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583745, Length=523, Percent_Identity=24.4741873804971, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24583749, Length=233, Percent_Identity=30.9012875536481, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583747, Length=233, Percent_Identity=30.9012875536481, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24586599, Length=223, Percent_Identity=32.7354260089686, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45549022, Length=221, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586597, Length=219, Percent_Identity=31.9634703196347, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281360393, Length=155, Percent_Identity=32.9032258064516, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 76085; Mature: 76085

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLVSFRDSEIFGRVAEVEFSLIREGSYAYLLGDFNAFNEGSFRMEQEGKNWKIKIALP
CCEEEECCCHHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCCEEEECCCCCEEEEEEEC
EGVWHYAFSIDGKFVLDPDNPERRVYTRKGYKFHREVNVARIVKSDDLVFHTPSLLYLYE
CCCEEEEEEECCEEEECCCCCCCEEEECCCEEEECCCCEEEEEECCCEEEECCHHHHHHH
IFGRVHVLLRTQKGVIKGATFLGEKHVPMRKKASDELFDYFEVIVEGGDKRLNYSFEVLT
HHHHHEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEE
MEGAKFEYGQFKARPFSIEFPTWVIDRVFYQIMPDKFARSRKIQGIAYPKDKYWGGDLIG
ECCCEEECCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCCEEECCCCCCCCCCEEE
IKEKIDHLVNLGINAIYLTPIFSSLTYHGYDIVDYFHVARRLGGDRAFVDLLSELKRFDI
HHHHHHHHHHCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCH
KVILDGVFHHTSFFHPYFQDVVRKGENSSFKNFYRIIKFPVVSKEFLQILHSKSSWEEKY
HHEEHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
KKIKSLGWNYESFFSVWIMPRLNHDNPKVREFIKNVILFWTNKGVDGFRMDVAHGVPPEV
HHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHEEEEECCCCCCEEEECCCCCCHHH
WKEVREALPKEKYLIGEVMDDARLWLFDKFHGVMNYRLYDAILRFFGYEEITAEEFLNEL
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ELLSSYYGPAEYLMYNFLDNHDVERFLDIVGDKRKYVCALVFLMTYKGIPSLFYGDEIGL
HHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
RGINLQGMESSRAPMLWNEEEWDQRILEITKTLVKIRKNNKALLFGNFVPVKFKRKFMVY
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEEEHHHHHHHHH
KREHMGERTIVAINYSNSRVKELGITIPEYSGVIINEDKVKLIKY
HHHCCCCEEEEEEECCCCCHHHHCCCCCCCCCEEECCCCEEEECC
>Mature Secondary Structure
MYKLVSFRDSEIFGRVAEVEFSLIREGSYAYLLGDFNAFNEGSFRMEQEGKNWKIKIALP
CCEEEECCCHHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCCEEEECCCCCEEEEEEEC
EGVWHYAFSIDGKFVLDPDNPERRVYTRKGYKFHREVNVARIVKSDDLVFHTPSLLYLYE
CCCEEEEEEECCEEEECCCCCCCEEEECCCEEEECCCCEEEEEECCCEEEECCHHHHHHH
IFGRVHVLLRTQKGVIKGATFLGEKHVPMRKKASDELFDYFEVIVEGGDKRLNYSFEVLT
HHHHHEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEE
MEGAKFEYGQFKARPFSIEFPTWVIDRVFYQIMPDKFARSRKIQGIAYPKDKYWGGDLIG
ECCCEEECCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCCEEECCCCCCCCCCEEE
IKEKIDHLVNLGINAIYLTPIFSSLTYHGYDIVDYFHVARRLGGDRAFVDLLSELKRFDI
HHHHHHHHHHCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCH
KVILDGVFHHTSFFHPYFQDVVRKGENSSFKNFYRIIKFPVVSKEFLQILHSKSSWEEKY
HHEEHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHH
KKIKSLGWNYESFFSVWIMPRLNHDNPKVREFIKNVILFWTNKGVDGFRMDVAHGVPPEV
HHHHHCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHEEEEECCCCCCEEEECCCCCCHHH
WKEVREALPKEKYLIGEVMDDARLWLFDKFHGVMNYRLYDAILRFFGYEEITAEEFLNEL
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ELLSSYYGPAEYLMYNFLDNHDVERFLDIVGDKRKYVCALVFLMTYKGIPSLFYGDEIGL
HHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
RGINLQGMESSRAPMLWNEEEWDQRILEITKTLVKIRKNNKALLFGNFVPVKFKRKFMVY
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEEEHHHHHHHHH
KREHMGERTIVAINYSNSRVKELGITIPEYSGVIINEDKVKLIKY
HHHCCCCEEEEEEECCCCCHHHHCCCCCCCCCEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]