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The map label for this gene is aegA [H]

Identifier: 18978282

GI number: 18978282

Start: 1760551

End: 1761981

Strand: Reverse

Name: aegA [H]

Synonym: PF1910

Alternate gene names: 18978282

Gene position: 1761981-1760551 (Counterclockwise)

Preceding gene: 18978283

Following gene: 18978281

Centisome position: 92.33

GC content: 43.89

Gene sequence:

>1431_bases
ATGCCTAGGCTTATTAAAGAGAGGGTTCCTACTCCTGAGAGGTCTGTTGGGGAGAGGGTTAGGGATTTTGGTGAGGTTAA
TCTTGGTTATTCTTGGGAGTTGGCTTTGAGGGAGGCTGAGCGTTGTCTCCAGTGTCCTGTTGAGTATGCCCCTTGCATTA
AAGGTTGTCCTGTGCATATTAACATTCCGGGTTTCATTAAAGCCTTGAGGGAGAATAGGGATAATCCTAGTAAGGCTGTG
AGGGAGGCTCTTAGGATTATTTGGAGGGATAATACGTTACCAGCAATTACTGGTAGGGTTTGTCCCCAGGAAGAGCAGTG
TGAGGGTGCTTGTGTGGTTGGAAAGGTTGGTGATCCAATAAACATTGGAAAATTGGAGAGGTTTGTGGCTGATTATGCTA
GGGAGCATGGGATTGACGATGAATTATTACTAGAGGAGATTAAGGGGATTAAGAGGAATGGGAAGAAAGTCGCAATCATT
GGAGCTGGGCCCGCTGGCCTAACTTGTGCCGCAGACCTGGCAAAAATGGGTTATGAGGTAACAATCTACGAAGCCCTCCA
CCAACCAGGAGGAGTACTAACATACGGAATCCCAGAATTCAGACTACCAAAAGAAATACTTAGAAAAGAGCTGAAGAAGC
TAAGCCTTTTGGGTGTAGAAATAAAAACGGACCATATAGTTGGTAAAACTATAACAATTCAAGAGTTACTTCAGGAATAT
GATGCAGTATTTATAGGAACTGGAGCTGGAACACCAAAATTGCCAAATATACCAGGAATAAACCTTAATGGTATTTATTC
TGCGAATGAGTTCCTCACGAGAATTAACCTAATGAAGGCCTACAAATTCCCAGAATACGACACTCCAATTGTTGTTGGGA
AGAAGGTTGTTGTTATTGGAGCTGGTAATACTGCAATGGATGCTGCTAGGTCAGCACTGAGGCTTGGAGCGGAGGTTACT
ATTGCTTACAGAAGAGGCAGGGAGGATATGACAGCCAGGATTGAGGAAGTTAAGCATGCTGAGGAAGAGGGAGTTAAGTT
CATGTTCTTTGTTAATCCAGTGGAATTCATTGGAGATGAGAATGGAAATGTTAAGGCGGTGAAGTTTGAGAAGATGAAGC
CACTGGAAGAGAGGGACAGTAGAGGAAAAAGAAAAATAGTTGGAACAGGAGAATACATAACTGTGGAAGCAGATACAGTA
ATAATAGCGATTGGCCAAACTCCAAATAAGGTTCTCTGGAGGGCAACTCCTGAGCTTAAGGTGGATGAGTGGGGTAGGAT
TGTTGTGGATGAGAATTTGATGACTAGTATTCCCGGGGTTTTTGCTGGTGGTGATGCTATTAGGGGTGAGGCTACGGTTA
TTCTAGCAATGGGCGACGGGAGAAAAGCAGCAAAAGCCATCCACCAATACCTAAGCAAGAAAGGAAATTGA

Upstream 100 bases:

>100_bases
TTGGGATGAGCTGATTCACAGAGTGGGGTTCTATGCCAAGTTGGAAAAATTAGCTCTGGAGAAATATATGGAAGAGCTTA
AGGCTAAGGGGGTGATTTGA

Downstream 100 bases:

>100_bases
CACCTCTTTCTTATAACCTTTTTAGGACGAAAGGTTTATATCTCCAGGATGATAGTAAAACTCTGGTGACGGCAAATGGC
GTGGAAGGTTTCTGTCGACC

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPRLIKERVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALRENRDNPSKAV
REALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAII
GAGPAGLTCAADLAKMGYEVTIYEALHQPGGVLTYGIPEFRLPKEILRKELKKLSLLGVEIKTDHIVGKTITIQELLQEY
DAVFIGTGAGTPKLPNIPGINLNGIYSANEFLTRINLMKAYKFPEYDTPIVVGKKVVVIGAGNTAMDAARSALRLGAEVT
IAYRRGREDMTARIEEVKHAEEEGVKFMFFVNPVEFIGDENGNVKAVKFEKMKPLEERDSRGKRKIVGTGEYITVEADTV
IIAIGQTPNKVLWRATPELKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKKGN

Sequences:

>Translated_476_residues
MPRLIKERVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALRENRDNPSKAV
REALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAII
GAGPAGLTCAADLAKMGYEVTIYEALHQPGGVLTYGIPEFRLPKEILRKELKKLSLLGVEIKTDHIVGKTITIQELLQEY
DAVFIGTGAGTPKLPNIPGINLNGIYSANEFLTRINLMKAYKFPEYDTPIVVGKKVVVIGAGNTAMDAARSALRLGAEVT
IAYRRGREDMTARIEEVKHAEEEGVKFMFFVNPVEFIGDENGNVKAVKFEKMKPLEERDSRGKRKIVGTGEYITVEADTV
IIAIGQTPNKVLWRATPELKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKKGN
>Mature_475_residues
PRLIKERVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALRENRDNPSKAVR
EALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAIIG
AGPAGLTCAADLAKMGYEVTIYEALHQPGGVLTYGIPEFRLPKEILRKELKKLSLLGVEIKTDHIVGKTITIQELLQEYD
AVFIGTGAGTPKLPNIPGINLNGIYSANEFLTRINLMKAYKFPEYDTPIVVGKKVVVIGAGNTAMDAARSALRLGAEVTI
AYRRGREDMTARIEEVKHAEEEGVKFMFFVNPVEFIGDENGNVKAVKFEKMKPLEERDSRGKRKIVGTGEYITVEADTVI
IAIGQTPNKVLWRATPELKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKKGN

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=33.9168490153173, Blast_Score=209, Evalue=6e-54,
Organism=Escherichia coli, GI1788811, Length=484, Percent_Identity=37.8099173553719, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789606, Length=475, Percent_Identity=39.1578947368421, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082180, Length=470, Percent_Identity=35.9574468085106, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1788468, Length=451, Percent_Identity=33.2594235033259, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789243, Length=421, Percent_Identity=34.6793349168646, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789463, Length=328, Percent_Identity=24.6951219512195, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984108, Length=482, Percent_Identity=33.8174273858921, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17570289, Length=489, Percent_Identity=31.4928425357873, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=31.0344827586207, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=488, Percent_Identity=29.5081967213115, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320584, Length=180, Percent_Identity=32.2222222222222, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=31.0204081632653, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=31.0204081632653, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=31.0204081632653, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=31.0204081632653, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24640763, Length=474, Percent_Identity=32.0675105485232, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI18858217, Length=474, Percent_Identity=32.0675105485232, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 52499; Mature: 52367

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLIKERVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHI
CCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEE
NIPGFIKALRENRDNPSKAVREALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPI
ECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCCEEEEECCCCCC
NIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAIIGAGPAGLTCAADLAKMGYEV
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCEE
TIYEALHQPGGVLTYGIPEFRLPKEILRKELKKLSLLGVEIKTDHIVGKTITIQELLQEY
EEEHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEEEECCEEECCEEEHHHHHHHC
DAVFIGTGAGTPKLPNIPGINLNGIYSANEFLTRINLMKAYKFPEYDTPIVVGKKVVVIG
CEEEEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEE
AGNTAMDAARSALRLGAEVTIAYRRGREDMTARIEEVKHAEEEGVKFMFFVNPVEFIGDE
CCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCC
NGNVKAVKFEKMKPLEERDSRGKRKIVGTGEYITVEADTVIIAIGQTPNKVLWRATPELK
CCCEEEEEECCCCCCHHHHCCCCEEEEECCCEEEEECCEEEEEECCCCCCEEEECCCCCC
VDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKKGN
CCCCCEEEECCCHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRLIKERVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHI
CCHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEE
NIPGFIKALRENRDNPSKAVREALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPI
ECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCCEEEEECCCCCC
NIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAIIGAGPAGLTCAADLAKMGYEV
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCEE
TIYEALHQPGGVLTYGIPEFRLPKEILRKELKKLSLLGVEIKTDHIVGKTITIQELLQEY
EEEHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEEEECCEEECCEEEHHHHHHHC
DAVFIGTGAGTPKLPNIPGINLNGIYSANEFLTRINLMKAYKFPEYDTPIVVGKKVVVIG
CEEEEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEE
AGNTAMDAARSALRLGAEVTIAYRRGREDMTARIEEVKHAEEEGVKFMFFVNPVEFIGDE
CCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCC
NGNVKAVKFEKMKPLEERDSRGKRKIVGTGEYITVEADTVIIAIGQTPNKVLWRATPELK
CCCEEEEEECCCCCCHHHHCCCCEEEEECCCEEEEECCEEEEEECCCCCCEEEECCCCCC
VDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKKGN
CCCCCEEEECCCHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]