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The map label for this gene is ogt

Identifier: 18978250

GI number: 18978250

Start: 1731133

End: 1731657

Strand: Direct

Name: ogt

Synonym: PF1878

Alternate gene names: 18978250

Gene position: 1731133-1731657 (Clockwise)

Preceding gene: 18978249

Following gene: 18978251

Centisome position: 90.72

GC content: 34.67

Gene sequence:

>525_bases
ATGATATTGGAAGTTAGGAAATTCCAAGTAAAAAATAAAGCAGTTTACATAGGGACATTAAGTGAGGACAAGATTTTTGG
AATTATATTTTCTATAGATGAGCCGGAAGTTATAAGGCATAGAATACCAACGCTAATTAACTTTCTTGAAAAAAGGTTAA
ACAAAAAACTTGAGATAAAGGAGGGAAATAGCGGATTTTCGGATGTTGTCTTTAAAACCCTTATAGGAAAAATAAGTAAC
GAAGAAGCAGCAGAGTTCATTGAAGTCTCCTATTTAACAAAATTTGAAAGAAAGCTGTATATCTATTTAGTGGAAAACGT
TAAAAGAGGAGAGGTAATAACCTATGGAGAGCTTGCAAAGATACTAAACACTTCATCAAGAGCAGTTGGAGCAGCTGTAA
AGAGAAATCCCTATCCAATAATAGTCCCCTGTCATCGAGTAATAGGCAGGAAAAATCCCTACTTGTATACTCCAAAACCC
GAATATAAAAAGTTCCTATTGGAGGTGGAAGGATGGACAAGCTAA

Upstream 100 bases:

>100_bases
TTTTCTATAATTGGAAGAGTCGAAAAAGGAGAAGGAGTGTATACAACAACAGGAGAAAAAGTGCCAATGCGTGGATGGGA
ACATTTTGCTGGTGGTAATA

Downstream 100 bases:

>100_bases
AGTTGTACGTAGCTGGATTTCTCGCTCTAATTATAGGTATAGCAGGATTTATAGTGTGGAAGTGGGGCTTTTGGATGCTA
ATTAGAATAATTTTAAGCCT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MILEVRKFQVKNKAVYIGTLSEDKIFGIIFSIDEPEVIRHRIPTLINFLEKRLNKKLEIKEGNSGFSDVVFKTLIGKISN
EEAAEFIEVSYLTKFERKLYIYLVENVKRGEVITYGELAKILNTSSRAVGAAVKRNPYPIIVPCHRVIGRKNPYLYTPKP
EYKKFLLEVEGWTS

Sequences:

>Translated_174_residues
MILEVRKFQVKNKAVYIGTLSEDKIFGIIFSIDEPEVIRHRIPTLINFLEKRLNKKLEIKEGNSGFSDVVFKTLIGKISN
EEAAEFIEVSYLTKFERKLYIYLVENVKRGEVITYGELAKILNTSSRAVGAAVKRNPYPIIVPCHRVIGRKNPYLYTPKP
EYKKFLLEVEGWTS
>Mature_174_residues
MILEVRKFQVKNKAVYIGTLSEDKIFGIIFSIDEPEVIRHRIPTLINFLEKRLNKKLEIKEGNSGFSDVVFKTLIGKISN
EEAAEFIEVSYLTKFERKLYIYLVENVKRGEVITYGELAKILNTSSRAVGAAVKRNPYPIIVPCHRVIGRKNPYLYTPKP
EYKKFLLEVEGWTS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_PYRFU (Q8TZV2)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579607.1
- ProteinModelPortal:   Q8TZV2
- SMR:   Q8TZV2
- EnsemblBacteria:   EBPYRT00000005624
- GeneID:   1469757
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1878
- NMPDR:   fig|186497.1.peg.1927
- GeneTree:   EBGT00050000022547
- HOGENOM:   HBG752398
- OMA:   KRNPYPI
- ProtClustDB:   PRK03887
- BRENDA:   2.1.1.63
- GO:   GO:0005737
- HAMAP:   MF_00772
- InterPro:   IPR015236
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- ProDom:   PD068053
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF09153 DUF1938; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 20100; Mature: 20100

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILEVRKFQVKNKAVYIGTLSEDKIFGIIFSIDEPEVIRHRIPTLINFLEKRLNKKLEIK
CEEECEEEEECCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEE
EGNSGFSDVVFKTLIGKISNEEAAEFIEVSYLTKFERKLYIYLVENVKRGEVITYGELAK
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEHHHHHH
ILNTSSRAVGAAVKRNPYPIIVPCHRVIGRKNPYLYTPKPEYKKFLLEVEGWTS
HHCCCCHHHHHHHCCCCCEEEEECHHHHCCCCCEEECCCHHHHEEEEEECCCCC
>Mature Secondary Structure
MILEVRKFQVKNKAVYIGTLSEDKIFGIIFSIDEPEVIRHRIPTLINFLEKRLNKKLEIK
CEEECEEEEECCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEE
EGNSGFSDVVFKTLIGKISNEEAAEFIEVSYLTKFERKLYIYLVENVKRGEVITYGELAK
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEHHHHHH
ILNTSSRAVGAAVKRNPYPIIVPCHRVIGRKNPYLYTPKPEYKKFLLEVEGWTS
HHCCCCHHHHHHHCCCCCEEEEECHHHHCCCCCEEECCCHHHHEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA