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The map label for this gene is trm1

Identifier: 18978243

GI number: 18978243

Start: 1726006

End: 1727151

Strand: Reverse

Name: trm1

Synonym: PF1871

Alternate gene names: 18978243

Gene position: 1727151-1726006 (Counterclockwise)

Preceding gene: 18978244

Following gene: 18978238

Centisome position: 90.51

GC content: 39.35

Gene sequence:

>1146_bases
ATGTCTATGGAATTATTTGAAGTTCATGAGGGGAAAGCTAAAGTTTTAGTTCCCAAAGCAAAAACTATTTATGATTCTCC
TGTTTTTTATAATCCTCGAATGGCTCCAAATAGGGATGTAGTTGTTTTGCTATTGAATGTTCTAAAACCTAAGATTGTTT
TGGATGCACTTTCTGCAACTGGAATTCGGGGAATTAGGTTTGCCTTAGAGACTCCTGCAGAAGAAATTTGGATGAATGAC
ATTAATGAATTAGCTTATGAACTCATGAAAAAGAACGTCCTATTAAACTTTAAGGGAACATTAAAAGAGAACGCTAAAAG
GGCAATTTTTGAAGGTGAAAAGACAATAGTAATCAATAATGATGATGCAAATAGGCTTATGGCAGAAAAGCATAGGTACT
TTCATTTCATAGATCTCGACCCTTTTGGGTCTCCAATGGAATTTTTAGATACCGCTCTTAGGAGCGTCAAAAGAAAGGGG
ATATTGGGAGTTACCGCTACCGACGGTGCTCCTCTTTGTGGAGCTCATCCAAAGGCATGCTTGAGGAAGTACCTAGCAGT
TCCCTTGAGGGGAGAACTGTGTCATGAGGTTGGAACAAGGATTTTAGTTGGAGTGATAGCTCGGTATGCTGCAAAGTATG
ACCTGGGAATGGAAGTGCTCCTTGCATATTACAAGGATCATTACTTTAGAGCATTTGTAAAACTTAAAGATGGGGCAAAG
AAAGGAGATGAGACTCTAGAAAATCTTGGATATGTTTACTTTGATGAAAAGACTGGAAGGTTTGAAGTGGAAAAATCTTT
CCTTTCGACTCGCCCAAACGCCTATGGTCCTCTTTGGCTTGGCCCTCTAAAGAATGAAAAAGTTGTGGGTGAAATGTTGG
AGCTCCTGGTGTCTGGATTTGAAGTTGCAAATTATAGAGAAGTGTTGAAGTTACTCCACATGCTTCATGAAGAGCTAGAC
ATCCCTCTATTCTATGATACCCACGCCCTGGGAAAAAGGCTCAAGATAGAACCAAAGAAACTTGGAGAAATAATAAAAGA
GCTAAAAAGCATGGGGTATGAGGCGACGAGAACACACTTTTCTCCAACTGGAATAAAGACTAACGCTCCTTACGAGGTTT
TTGTTGAAGTAATGAGGAAAAATTAG

Upstream 100 bases:

>100_bases
ATAGTTAGAGTTCATGGGACAAAGGGGGCAGTAAGGGCCAGGTTTGAAAAAGGACTTCCAGGACAGGCCTTAGGAGATTA
TGTGGAAATAGTCTAATGGG

Downstream 100 bases:

>100_bases
CGTTTTCTTGTCCATTCAAATGCAAAAGTTGCTATAAAACCTAGGAGGAATGAAAATAATACTGGGAACTTTTCATACAG
CATGAATGAAAGTGCAAATC

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSATGIRGIRFALETPAEEIWMND
INELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKG
ILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAK
KGDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVANYREVLKLLHMLHEELD
IPLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFSPTGIKTNAPYEVFVEVMRKN

Sequences:

>Translated_381_residues
MSMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSATGIRGIRFALETPAEEIWMND
INELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKG
ILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAK
KGDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVANYREVLKLLHMLHEELD
IPLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFSPTGIKTNAPYEVFVEVMRKN
>Mature_380_residues
SMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSATGIRGIRFALETPAEEIWMNDI
NELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGI
LGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAKK
GDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVANYREVLKLLHMLHEELDI
PLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFSPTGIKTNAPYEVFVEVMRKN

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI216548445, Length=334, Percent_Identity=30.5389221556886, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI8923219, Length=359, Percent_Identity=28.4122562674095, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI209862871, Length=359, Percent_Identity=28.4122562674095, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI163792194, Length=297, Percent_Identity=31.986531986532, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17564890, Length=447, Percent_Identity=29.9776286353468, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320325, Length=466, Percent_Identity=26.8240343347639, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19921190, Length=443, Percent_Identity=27.0880361173815, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_PYRFU (P81554)

Other databases:

- EMBL:   AF051912
- EMBL:   AE009950
- PIR:   T51778
- RefSeq:   NP_579600.1
- ProteinModelPortal:   P81554
- SMR:   P81554
- EnsemblBacteria:   EBPYRT00000005501
- GeneID:   1469750
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1871
- NMPDR:   fig|186497.1.peg.1920
- GeneTree:   EBGT00050000022754
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- ProtClustDB:   PRK04338
- BRENDA:   2.1.1.32
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 43319; Mature: 43188

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSAT
CCCEEEEECCCCEEEEECCCHHHCCCCCEECCCCCCCCCHHHHHHHHHCHHHHHHHHHHC
GIRGIRFALETPAEEIWMNDINELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINN
CCCCEEEEHHCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEEEC
DDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKAC
CCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
LRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHECCCCC
KGDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGF
CHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCC
EVANYREVLKLLHMLHEELDIPLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHF
CCCCHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCC
SPTGIKTNAPYEVFVEVMRKN
CCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSAT
CCEEEEECCCCEEEEECCCHHHCCCCCEECCCCCCCCCHHHHHHHHHCHHHHHHHHHHC
GIRGIRFALETPAEEIWMNDINELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINN
CCCCEEEEHHCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEEEC
DDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKAC
CCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
LRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHECCCCC
KGDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGF
CHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHCC
EVANYREVLKLLHMLHEELDIPLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHF
CCCCHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCC
SPTGIKTNAPYEVFVEVMRKN
CCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9685492; 10438627