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The map label for this gene is aprX [H]

Identifier: 18978042

GI number: 18978042

Start: 1553191

End: 1555155

Strand: Reverse

Name: aprX [H]

Synonym: PF1670

Alternate gene names: 18978042

Gene position: 1555155-1553191 (Counterclockwise)

Preceding gene: 18978043

Following gene: 18978041

Centisome position: 81.5

GC content: 44.17

Gene sequence:

>1965_bases
ATGAAGGGGCTAAAAGCTCTCATATTAGTGATTTTAGTTCTAGGTTTGGTAGTAGGGAGCGTAGCGGCAGCTCCAGAGAA
GAAAGTTGAACAAGTAAGAAATGTTGAGAAGAACTATGGTCTGCTAACGCCAGGACTGTTCAGAAAAATTCAAAAATTGA
ATCCTAACGAGGAAATCAGCACAGTAATTGTATTTGAAAACCATAGGGAAAAAGAAATTGCAGTAAGAGTTCTTGAGTTA
ATGGGTGCAAAAGTTAGGTATGTGTACCATATTATACCCGCAATAGCTGCCGATCTTAAGGTTAGAGACTTACTAGTCAT
CTCAGGTTTAACAGGGGGTAAAGCTAAGCTTTCAGGTGTTAGGTTTATCCAGGAAGACTACAAAGTTACAGTTTCAGCAG
AATTAGAAGGACTGGATGAGTCTGCAGCTCAAGTTATGGCAACTTACGTTTGGAACTTGGGATATGATGGTTCTGGAATC
ACAATAGGAATAATTGACACTGGAATTGACGCTTCTCATCCAGATCTCCAAGGAAAAGTAATTGGGTGGGTAGATTTTGT
CAATGGTAGGAGTTATCCATACGATGACCATGGACATGGAACTCATGTAGCTTCAATAGCAGCTGGTACTGGAGCAGCAA
GTAATGGCAAGTACAAGGGAATGGCTCCAGGAGCTAAGCTGGCGGGAATTAAGGTTCTAGGTGCCGATGGTTCTGGAAGC
ATATCTACTATAATTAAGGGAGTTGAGTGGGCCGTTGATAACAAAGATAAGTACGGAATTAAGGTCATTAATCTTTCTCT
TGGTTCAAGCCAGAGCTCAGATGGTACTGACGCTCTAAGTCAGGCTGTTAATGCAGCGTGGGATGCTGGATTAGTTGTTG
TGGTTGCCGCTGGAAACAGTGGACCTAACAAGTATACAATCGGTTCTCCAGCAGCTGCAAGCAAAGTTATTACAGTTGGA
GCCGTTGACAAGTATGATGTTATAACAAGCTTCTCAAGCAGAGGGCCAACTGCAGACGGCAGGCTTAAGCCTGAGGTTGT
TGCTCCAGGAAACTGGATAATTGCTGCCAGAGCAAGTGGAACTAGCATGGGTCAACCAATTAATGACTATTACACAGCAG
CTCCTGGGACATCAATGGCAACTCCTCACGTAGCTGGTATTGCAGCCCTCTTGCTCCAAGCACACCCGAGCTGGACTCCA
GACAAAGTAAAAACAGCCCTCATAGAAACTGCTGATATCGTAAAGCCAGATGAAATAGCCGATATAGCCTACGGTGCAGG
TAGGGTTAATGCATACAAGGCTATAAACTACGATAACTATGCAAAGCTAGTGTTCACTGGATATGTTGCCAACAAAGGCA
GCCAAACTCACCAGTTCGTTATTAGCGGAGCTTCGTTCGTAACTGCCACATTATACTGGGACAATGCCAATAGCGACCTT
GATCTTTACCTCTACGATCCCAATGGAAACCAGGTTGACTACTCTTACACCGCCTACTATGGATTCGAAAAGGTTGGTTA
TTACAACCCAACTGATGGAACATGGACAATTAAGGTTGTAAGCTACAGCGGAAGTGCAAACTATCAAGTAGATGTGGTAA
GTGATGGTTCCCTTTCACAGCCTGGAAGTTCACCATCTCCACAACCAGAACCAACAGTAGACGCAAAGACGTTCCAAGGA
TCCGTTCACTACTACTATGACAGGAGCGACACCTTTACAATGACCGTTAACTCTGGGGCTACAAAGATTACTGGAGACCT
AGTGTTTGACACAAGCTACCATGATCTTGACCTTTACCTCTACGATCCTAACCAGAAGCTTGTAGATAGATCGGAGAGTT
CCAACAGCTACGAACACGTAGAATACTTAAACCCCGCCCCAGGAACCTGGTACTTCCTAGTATATGCCTACTACACTTAC
GGTTGGGCTTACTACGAGCTGACGGCTAAAGTTTATTATGGCTGA

Upstream 100 bases:

>100_bases
ACTATATGTAATATATCCTCATTAGAACTGTTGACACCAAAATATTTATTAAGCATAAAATAACCATGCAACTTTGATCA
CTAATGTACGGTGGTGCCAC

Downstream 100 bases:

>100_bases
AGCCTTTTAACTATTCTTTCTTTTTTGTTTTAGGGAGGTGAATTTTTGTGAAAAAGGTATTCTTAGGGGTAATTATCATT
ACTTTACTCTTATCTCCAGT

Product: alkaline serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MKGLKALILVILVLGLVVGSVAAAPEKKVEQVRNVEKNYGLLTPGLFRKIQKLNPNEEISTVIVFENHREKEIAVRVLEL
MGAKVRYVYHIIPAIAADLKVRDLLVISGLTGGKAKLSGVRFIQEDYKVTVSAELEGLDESAAQVMATYVWNLGYDGSGI
TIGIIDTGIDASHPDLQGKVIGWVDFVNGRSYPYDDHGHGTHVASIAAGTGAASNGKYKGMAPGAKLAGIKVLGADGSGS
ISTIIKGVEWAVDNKDKYGIKVINLSLGSSQSSDGTDALSQAVNAAWDAGLVVVVAAGNSGPNKYTIGSPAAASKVITVG
AVDKYDVITSFSSRGPTADGRLKPEVVAPGNWIIAARASGTSMGQPINDYYTAAPGTSMATPHVAGIAALLLQAHPSWTP
DKVKTALIETADIVKPDEIADIAYGAGRVNAYKAINYDNYAKLVFTGYVANKGSQTHQFVISGASFVTATLYWDNANSDL
DLYLYDPNGNQVDYSYTAYYGFEKVGYYNPTDGTWTIKVVSYSGSANYQVDVVSDGSLSQPGSSPSPQPEPTVDAKTFQG
SVHYYYDRSDTFTMTVNSGATKITGDLVFDTSYHDLDLYLYDPNQKLVDRSESSNSYEHVEYLNPAPGTWYFLVYAYYTY
GWAYYELTAKVYYG

Sequences:

>Translated_654_residues
MKGLKALILVILVLGLVVGSVAAAPEKKVEQVRNVEKNYGLLTPGLFRKIQKLNPNEEISTVIVFENHREKEIAVRVLEL
MGAKVRYVYHIIPAIAADLKVRDLLVISGLTGGKAKLSGVRFIQEDYKVTVSAELEGLDESAAQVMATYVWNLGYDGSGI
TIGIIDTGIDASHPDLQGKVIGWVDFVNGRSYPYDDHGHGTHVASIAAGTGAASNGKYKGMAPGAKLAGIKVLGADGSGS
ISTIIKGVEWAVDNKDKYGIKVINLSLGSSQSSDGTDALSQAVNAAWDAGLVVVVAAGNSGPNKYTIGSPAAASKVITVG
AVDKYDVITSFSSRGPTADGRLKPEVVAPGNWIIAARASGTSMGQPINDYYTAAPGTSMATPHVAGIAALLLQAHPSWTP
DKVKTALIETADIVKPDEIADIAYGAGRVNAYKAINYDNYAKLVFTGYVANKGSQTHQFVISGASFVTATLYWDNANSDL
DLYLYDPNGNQVDYSYTAYYGFEKVGYYNPTDGTWTIKVVSYSGSANYQVDVVSDGSLSQPGSSPSPQPEPTVDAKTFQG
SVHYYYDRSDTFTMTVNSGATKITGDLVFDTSYHDLDLYLYDPNQKLVDRSESSNSYEHVEYLNPAPGTWYFLVYAYYTY
GWAYYELTAKVYYG
>Mature_654_residues
MKGLKALILVILVLGLVVGSVAAAPEKKVEQVRNVEKNYGLLTPGLFRKIQKLNPNEEISTVIVFENHREKEIAVRVLEL
MGAKVRYVYHIIPAIAADLKVRDLLVISGLTGGKAKLSGVRFIQEDYKVTVSAELEGLDESAAQVMATYVWNLGYDGSGI
TIGIIDTGIDASHPDLQGKVIGWVDFVNGRSYPYDDHGHGTHVASIAAGTGAASNGKYKGMAPGAKLAGIKVLGADGSGS
ISTIIKGVEWAVDNKDKYGIKVINLSLGSSQSSDGTDALSQAVNAAWDAGLVVVVAAGNSGPNKYTIGSPAAASKVITVG
AVDKYDVITSFSSRGPTADGRLKPEVVAPGNWIIAARASGTSMGQPINDYYTAAPGTSMATPHVAGIAALLLQAHPSWTP
DKVKTALIETADIVKPDEIADIAYGAGRVNAYKAINYDNYAKLVFTGYVANKGSQTHQFVISGASFVTATLYWDNANSDL
DLYLYDPNGNQVDYSYTAYYGFEKVGYYNPTDGTWTIKVVSYSGSANYQVDVVSDGSLSQPGSSPSPQPEPTVDAKTFQG
SVHYYYDRSDTFTMTVNSGATKITGDLVFDTSYHDLDLYLYDPNQKLVDRSESSNSYEHVEYLNPAPGTWYFLVYAYYTY
GWAYYELTAKVYYG

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=317, Percent_Identity=30.9148264984227, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI186972143, Length=285, Percent_Identity=32.6315789473684, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI31317307, Length=368, Percent_Identity=28.804347826087, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17533391, Length=238, Percent_Identity=31.0924369747899, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324576, Length=302, Percent_Identity=34.1059602649007, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320775, Length=285, Percent_Identity=35.7894736842105, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6319893, Length=251, Percent_Identity=37.0517928286853, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550681, Length=305, Percent_Identity=29.5081967213115, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653270, Length=298, Percent_Identity=30.5369127516779, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17137360, Length=298, Percent_Identity=30.5369127516779, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 70231; Mature: 70231

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLKALILVILVLGLVVGSVAAAPEKKVEQVRNVEKNYGLLTPGLFRKIQKLNPNEEIS
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEE
TVIVFENHREKEIAVRVLELMGAKVRYVYHIIPAIAADLKVRDLLVISGLTGGKAKLSGV
EEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEHHHE
RFIQEDYKVTVSAELEGLDESAAQVMATYVWNLGYDGSGITIGIIDTGIDASHPDLQGKV
EEEECCCEEEEEEEECCCCHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCCCCCCEE
IGWVDFVNGRSYPYDDHGHGTHVASIAAGTGAASNGKYKGMAPGAKLAGIKVLGADGSGS
EEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
ISTIIKGVEWAVDNKDKYGIKVINLSLGSSQSSDGTDALSQAVNAAWDAGLVVVVAAGNS
HHHHHCCCEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCC
GPNKYTIGSPAAASKVITVGAVDKYDVITSFSSRGPTADGRLKPEVVAPGNWIIAARASG
CCCEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEECCC
TSMGQPINDYYTAAPGTSMATPHVAGIAALLLQAHPSWTPDKVKTALIETADIVKPDEIA
CCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHH
DIAYGAGRVNAYKAINYDNYAKLVFTGYVANKGSQTHQFVISGASFVTATLYWDNANSDL
HHHCCCCCCEEEEEECCCCEEEEEEEEEEECCCCCCEEEEEECCCEEEEEEEECCCCCCE
DLYLYDPNGNQVDYSYTAYYGFEKVGYYNPTDGTWTIKVVSYSGSANYQVDVVSDGSLSQ
EEEEECCCCCEEEEEEEEEECHHHCCEECCCCCEEEEEEEEECCCCCEEEEEEECCCCCC
PGSSPSPQPEPTVDAKTFQGSVHYYYDRSDTFTMTVNSGATKITGDLVFDTSYHDLDLYL
CCCCCCCCCCCCCCCEEEECCEEEEEECCCEEEEEECCCCEEEEEEEEEECCCCEEEEEE
YDPNQKLVDRSESSNSYEHVEYLNPAPGTWYFLVYAYYTYGWAYYELTAKVYYG
ECCCHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEEHHHHCEEEEEEEEEEECC
>Mature Secondary Structure
MKGLKALILVILVLGLVVGSVAAAPEKKVEQVRNVEKNYGLLTPGLFRKIQKLNPNEEIS
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEE
TVIVFENHREKEIAVRVLELMGAKVRYVYHIIPAIAADLKVRDLLVISGLTGGKAKLSGV
EEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEHHHE
RFIQEDYKVTVSAELEGLDESAAQVMATYVWNLGYDGSGITIGIIDTGIDASHPDLQGKV
EEEECCCEEEEEEEECCCCHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCCCCCCEE
IGWVDFVNGRSYPYDDHGHGTHVASIAAGTGAASNGKYKGMAPGAKLAGIKVLGADGSGS
EEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
ISTIIKGVEWAVDNKDKYGIKVINLSLGSSQSSDGTDALSQAVNAAWDAGLVVVVAAGNS
HHHHHCCCEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCC
GPNKYTIGSPAAASKVITVGAVDKYDVITSFSSRGPTADGRLKPEVVAPGNWIIAARASG
CCCEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEECCC
TSMGQPINDYYTAAPGTSMATPHVAGIAALLLQAHPSWTPDKVKTALIETADIVKPDEIA
CCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHH
DIAYGAGRVNAYKAINYDNYAKLVFTGYVANKGSQTHQFVISGASFVTATLYWDNANSDL
HHHCCCCCCEEEEEECCCCEEEEEEEEEEECCCCCCEEEEEECCCEEEEEEEECCCCCCE
DLYLYDPNGNQVDYSYTAYYGFEKVGYYNPTDGTWTIKVVSYSGSANYQVDVVSDGSLSQ
EEEEECCCCCEEEEEEEEEECHHHCCEECCCCCEEEEEEEEECCCCCEEEEEEECCCCCC
PGSSPSPQPEPTVDAKTFQGSVHYYYDRSDTFTMTVNSGATKITGDLVFDTSYHDLDLYL
CCCCCCCCCCCCCCCEEEECCEEEEEECCCEEEEEECCCCEEEEEEEEEECCCCEEEEEE
YDPNQKLVDRSESSNSYEHVEYLNPAPGTWYFLVYAYYTYGWAYYELTAKVYYG
ECCCHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEEHHHHCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]