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The map label for this gene is rpoN [H]

Identifier: 18978015

GI number: 18978015

Start: 1533155

End: 1533367

Strand: Reverse

Name: rpoN [H]

Synonym: PF1643

Alternate gene names: 18978015

Gene position: 1533367-1533155 (Counterclockwise)

Preceding gene: 18978016

Following gene: 18978014

Centisome position: 80.35

GC content: 41.31

Gene sequence:

>213_bases
ATGGGGGCAAGTCCCGTGATTGTACCTGTTAGATGTTTCACATGTGGTAAGGTGATTGGAGATAAATACTATGAGTTTAA
AAGAAGGGTAGAAGCTGGGGAAGATCCCGAAAAGGTCCTTGACGATTTGGGTTTAGAAAGGTACTGCTGTAGGAGAATGC
TCCTAAGTCATGTAGAGCTAATTGATGACATAATGCATTACAGAGTGTATTGA

Upstream 100 bases:

>100_bases
AGCCAAACAGATCTACAAAGGGTCCAAGAGCTAAGAGACAGAAGAGTTATCGTTGAACTCTTTCCGTTAATTTTAAAAAC
TCTTTTATTATCGAAAACGG

Downstream 100 bases:

>100_bases
AAAAACCACCCTTTCTTTATTTAAGTGGGGCCGTGGGGTAGCTTGGCTATCCTCCCGGCCTGGGGCGCCGGAGACCCGGG
TTCAAATCCCGGCGGCCCCA

Product: DNA-directed RNA polymerase subunit N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MGASPVIVPVRCFTCGKVIGDKYYEFKRRVEAGEDPEKVLDDLGLERYCCRRMLLSHVELIDDIMHYRVY

Sequences:

>Translated_70_residues
MGASPVIVPVRCFTCGKVIGDKYYEFKRRVEAGEDPEKVLDDLGLERYCCRRMLLSHVELIDDIMHYRVY
>Mature_69_residues
GASPVIVPVRCFTCGKVIGDKYYEFKRRVEAGEDPEKVLDDLGLERYCCRRMLLSHVELIDDIMHYRVY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG1644

COG function: function code K; DNA-directed RNA polymerase, subunit N (RpoN/RPB10)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family [H]

Homologues:

Organism=Homo sapiens, GI10863925, Length=62, Percent_Identity=51.6129032258064, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17509857, Length=62, Percent_Identity=53.2258064516129, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324784, Length=63, Percent_Identity=52.3809523809524, Blast_Score=71, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24649759, Length=62, Percent_Identity=53.2258064516129, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR000268
- InterPro:   IPR020789
- ProDom:   PD006539 [H]

Pfam domain/function: PF01194 RNA_pol_N [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 8199; Mature: 8067

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS01112 RNA_POL_N_8KD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
10.0 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASPVIVPVRCFTCGKVIGDKYYEFKRRVEAGEDPEKVLDDLGLERYCCRRMLLSHVEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
IDDIMHYRVY
HHHHHHHHCC
>Mature Secondary Structure 
GASPVIVPVRCFTCGKVIGDKYYEFKRRVEAGEDPEKVLDDLGLERYCCRRMLLSHVEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
IDDIMHYRVY
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]