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Identifier: 18978007

GI number: 18978007

Start: 1525737

End: 1527422

Strand: Direct

Name: lig

Synonym: PF1635

Alternate gene names: 18978007

Gene position: 1525737-1527422 (Clockwise)

Preceding gene: 18978004

Following gene: 18978024

Centisome position: 79.95

GC content: 41.22

Gene sequence:

>1686_bases
ATGAGGTATCTAGAGCTTGCTCAACTTTATCAAAAGTTAGAAAAGACAACTATGAAACTTATAAAGACTAGACTTGTCGC
CGACTTCCTGAAAAAAGTACCAGATGATCATCTGGAGTTCATTCCCTATCTAATTCTTGGAGAAGTTTTTCCAGAGTGGG
ATGAAAGGGAGCTGGGTGTGGGAGAAAAGCTGTTAATTAAAGCTGTAGCAATGGCCACTGGAATTGACGCAAAAGAAATC
GAAGAGTCTGTAAAAGATACTGGAGACCTTGGAGAGAGCATAGCCTTAGCTGTAAAGAAAAAGAAGCAGAAGAGCTTCTT
CTCTCAGCCCCTCACAATAAAGAGGGTATATCAAACCCTTGTAAAGGTTGCAGAAACAACGGGGGAGGGAAGCCAAGATA
AAAAAGTAAAGTATCTAGCTGATTTGTTCATGGACGCAGAACCTTTAGAAGCTAAGTATCTTGCTCGTACAATCTTAGGA
ACAATGAGAACAGGAGTTGCAGAAGGATTGCTTAGAGATGCAATAGCAATGGCATTCCACGTAAAGGTAGAGCTTGTTGA
GAGAGCTTACATGCTAACGAGTGATTTCGGATATGTAGCTAAAATAGCAAAGCTTGAAGGAAATGAAGGGCTAGCAAAAG
TTCAAGTTCAACTCGGAAAGCCAATAAAGCCAATGCTTGCCCAGCAAGCTGCTAGCATAAGAGATGCACTTCTCGAGATG
GGTGGAGAGGCAGAGTTCGAGATTAAATACGATGGAGCAAGGGTGCAGGTGCACAAGGATGGCTCAAAAATTATAGTCTA
TTCTAGAAGACTGGAGAACGTCACCAGAGCGATTCCAGAAATTGTTGAGGCTCTAAAAGAGGCAATAATACCTGAAAAGG
CAATAGTGGAAGGAGAACTTGTGGCAATTGGAGAAAACGGAAGACCATTGCCCTTCCAATATGTGCTTAGAAGGTTTAGG
AGAAAGCATAACATAGAAGAAATGATGGAAAAGATACCTCTCGAGCTCAACTTATTCGACGTTCTCTACGTAGATGGACA
AAGCTTGATTGACACTAAGTTCATTGATAGAAGAAGAACACTTGAAGAAATAATAAAGCAGAATGAAAAGATAAAGGTAG
CAGAAAACCTAATAACAAAGAAAGTCGAGGAAGCAGAGGCATTTTACAAGAGAGCACTCGAAATGGGGCACGAGGGATTG
ATGGCCAAGAGGTTAGATGCAGTCTACGAACCAGGTAACAGAGGAAAGAAGTGGTTGAAGATAAAGCCCACAATGGAGAA
CTTAGATTTAGTAATCATAGGAGCAGAATGGGGAGAGGGAAGAAGAGCCCATCTCTTTGGTTCATTCATCCTGGGAGCAT
ATGATCCAGAAACAGGAGAATTCCTAGAGGTAGGAAAAGTGGGAAGTGGATTCACAGATGATGACTTAGTTGAGTTTACG
AAGATGCTAAAGCCCCTTATTATAAAAGAGGAAGGAAAGAGAGTCTGGCTCCAGCCCAAAGTTGTTATTGAAGTGACATA
TCAAGAAATTCAGAAGAGTCCAAAATACAGAAGTGGATTTGCATTAAGGTTCCCAAGGTTCGTTGCACTTAGAGATGATA
AAGGACCAGAAGATGCAGATACAATAGAGAGAATCGCACAACTTTACGAGTTGCAAGAAAAGATGAAAGGAAAAGTGGAA
AGCTAA

Upstream 100 bases:

>100_bases
GGAGAGGTACTATGAATGAACTTAAGTGATCATATGGATTTTTTGCCTAACATATTCTTTTTATACTTTTTTTGACTATC
TTTCTAAGTGGGGGGCAAAG

Downstream 100 bases:

>100_bases
GGTCTAACAATAGTCCCGATACCATTTCCAACTATCGCCATTGAAAGTCTATCCTTGACTAAACCATTGATAAGCCAAAC
CTCCTCAGTATAATGGACTA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Pfu DNA ligase; Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGVGEKLLIKAVAMATGIDAKEI
EESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTLVKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILG
TMRTGVAEGLLRDAIAMAFHVKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEM
GGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGELVAIGENGRPLPFQYVLRRFR
RKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRTLEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGL
MAKRLDAVYEPGNRGKKWLKIKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFT
KMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDADTIERIAQLYELQEKMKGKVE
S

Sequences:

>Translated_561_residues
MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGVGEKLLIKAVAMATGIDAKEI
EESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTLVKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILG
TMRTGVAEGLLRDAIAMAFHVKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEM
GGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGELVAIGENGRPLPFQYVLRRFR
RKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRTLEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGL
MAKRLDAVYEPGNRGKKWLKIKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFT
KMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDADTIERIAQLYELQEKMKGKVE
S
>Mature_561_residues
MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGVGEKLLIKAVAMATGIDAKEI
EESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTLVKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILG
TMRTGVAEGLLRDAIAMAFHVKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEM
GGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGELVAIGENGRPLPFQYVLRRFR
RKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRTLEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGL
MAKRLDAVYEPGNRGKKWLKIKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFT
KMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDADTIERIAQLYELQEKMKGKVE
S

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=623, Percent_Identity=30.4975922953451, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI73747829, Length=575, Percent_Identity=26.7826086956522, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI73747844, Length=576, Percent_Identity=26.7361111111111, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI46255052, Length=513, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI23199993, Length=513, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI148539894, Length=513, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71985269, Length=606, Percent_Identity=32.8382838283828, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71985278, Length=182, Percent_Identity=32.4175824175824, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320038, Length=614, Percent_Identity=31.5960912052117, Blast_Score=256, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6324578, Length=445, Percent_Identity=23.5955056179775, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24762424, Length=619, Percent_Identity=30.048465266559, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI85815841, Length=558, Percent_Identity=26.5232974910394, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24641829, Length=523, Percent_Identity=23.9005736137667, Blast_Score=94, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_PYRFU (P56709)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579364.1
- PDB:   2CFM
- PDBsum:   2CFM
- ProteinModelPortal:   P56709
- SMR:   P56709
- DIP:   DIP-48778N
- EnsemblBacteria:   EBPYRT00000004661
- GeneID:   1469512
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1635
- NMPDR:   fig|186497.1.peg.1683
- GeneTree:   EBGT00050000022632
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- ProtClustDB:   PRK01109
- BRENDA:   6.5.1.1
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 63774; Mature: 63774

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 249-249 BINDING 247-247 BINDING 254-254 BINDING 269-269 BINDING 299-299 BINDING 339-339 BINDING 414-414 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GEKLLIKAVAMATGIDAKEIEESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILGTMRTGVAEGLLRDAIAMAFH
HHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEM
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGEL
CCCEEEEEEECCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCE
VAIGENGRPLPFQYVLRRFRRKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRT
EEECCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHH
LEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGLMAKRLDAVYEPGNRGKKWLK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCEEEE
IKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFT
ECCCCCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHH
KMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDAD
HHHHHHEEECCCCEEEECCEEEEEEEHHHHHCCCCCCCCCEEECCEEEEEECCCCCCCHH
TIERIAQLYELQEKMKGKVES
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GEKLLIKAVAMATGIDAKEIEESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILGTMRTGVAEGLLRDAIAMAFH
HHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEM
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGEL
CCCEEEEEEECCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCE
VAIGENGRPLPFQYVLRRFRRKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRT
EEECCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHH
LEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGLMAKRLDAVYEPGNRGKKWLK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCEEEE
IKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFT
ECCCCCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHH
KMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDAD
HHHHHHEEECCCCEEEECCEEEEEEEHHHHHCCCCCCCCCEEECCEEEEEECCCCCCCHH
TIERIAQLYELQEKMKGKVES
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA