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The map label for this gene is prf1

Identifier: 18977965

GI number: 18977965

Start: 1487345

End: 1488607

Strand: Reverse

Name: prf1

Synonym: PF1593

Alternate gene names: 18977965

Gene position: 1488607-1487345 (Counterclockwise)

Preceding gene: 18977971

Following gene: 18977960

Centisome position: 78.01

GC content: 41.01

Gene sequence:

>1263_bases
GTGAGAGTTATGACTTCTCGTGACGCTCAACTTTATGAATTAAAGAAAAAGATTGATGAGCTGAAAAAAATAAGAGGGAG
GGGTACAGAGCTTATCTCTCTCTACATTCCAGCGGGATATGACTTAAGTAAAGTTATGCAACAGCTTAGAGAGGAATACA
GCACGGCTCAGAACATAAAATCAAAAACAACAAGGAAAAATGTGCTAGGAGCTCTTGAAAGGGCAATGCAACATCTCAAG
CTTTACAAGCAAACACCAGAGAACGGATTAGCTCTGTTTGTTGGAAACGTGAGCGAAATGGAAGGAGTTACTGACATAAG
GCTTTGGGCAATAATTCCTCCTGAGCCGTTAAACGTAAGACTTTATCGATGTGATCAAACGTTTGTTACGGAACCTCTTG
AAGAGATGCTGAGAGTAAAGGAGGCCTACGGATTAATAACAGTGGAAAAAAATGAAGCTACAATAGGAATTTTAAGGGGA
AAGAGAATAGAAGTTCTAGATGAATTAACTTCGAATGTTCCAGGAAAGACAAGAGCTGGAGGTCAGTCAGCTCGAAGATA
TGAGCGAATTAGAGAGCAGGAAACCCACGAGTTCATGAAGAGAATTGGAGAGCATGCAAATAGGATATTCCTTCCTCTGT
TAGAAAGTGGAGAGCTAAAGGGAATAATTGTAGGAGGACCAGGACCAACTAAAGAAGAGTTTGTAGAGGGGGATTATCTC
CATCACGAGCTCAAGAAGAAAATAATAGGCATTGTAGATATAAGTTATCACGGAGAATATGGATTAAGAGAGTTGGTAGC
AAAAGCTGCAGATATTTTAAGGGATCATGAAGTCATAAGAGAGAGAAATCTTGTCAATGAATTCCTAAAGCACATAGTAA
AGGATACTGGCCTAGCTACCTACGGTGAGAGAGAAGTAAGAAAGGCCCTTGAGCTTGGAGCTGTGGATACGCTCCTTATC
AGTGAAGGCTATGACAAGGTTAGGGTTCACGTCAAGTGTAATAATTGTGGATGGGAAGAGCTGAAGACAATGAGTGAGGA
GGAGTACGAGGCATACAAAAAGAGAATACAAACATGTCCAAAGTGTGGAAGCCAGAATCTTACCTTCGAAAAATGGGAGG
TAGCAGAAGAGTTAATAAAAATAGCAGAGGAAGCAGGGTCAAATGTTGAAATTATATCTCTCGACACTGAGGAAGGCCAA
CAGTTTTATAGGGCCTTTGGAGGTCTTGGGGCAATCCTAAGGTTTAGGATTCAAGGGGTTTGA

Upstream 100 bases:

>100_bases
GTAATTTGATTTCTCTCAGAATCATGCCTCCCACCAATGCCAAACCTTTATAAATCAGCCAATTTTTAAAGTTTTAGACG
CGATAAAGAGGTTTAATGGG

Downstream 100 bases:

>100_bases
TATGGGAAGATCTTTAGGCTCGTAGTCTTGGAGCCTTCCCTCCAAGTATTCTTCGTATCCATGGAGATCTAGTAGGCCGT
GGCCGCTTAAGTTGAACAAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRVMTSRDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTAQNIKSKTTRKNVLGALERAMQHLK
LYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLNVRLYRCDQTFVTEPLEEMLRVKEAYGLITVEKNEATIGILRG
KRIEVLDELTSNVPGKTRAGGQSARRYERIREQETHEFMKRIGEHANRIFLPLLESGELKGIIVGGPGPTKEEFVEGDYL
HHELKKKIIGIVDISYHGEYGLRELVAKAADILRDHEVIRERNLVNEFLKHIVKDTGLATYGEREVRKALELGAVDTLLI
SEGYDKVRVHVKCNNCGWEELKTMSEEEYEAYKKRIQTCPKCGSQNLTFEKWEVAEELIKIAEEAGSNVEIISLDTEEGQ
QFYRAFGGLGAILRFRIQGV

Sequences:

>Translated_420_residues
MRVMTSRDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTAQNIKSKTTRKNVLGALERAMQHLK
LYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLNVRLYRCDQTFVTEPLEEMLRVKEAYGLITVEKNEATIGILRG
KRIEVLDELTSNVPGKTRAGGQSARRYERIREQETHEFMKRIGEHANRIFLPLLESGELKGIIVGGPGPTKEEFVEGDYL
HHELKKKIIGIVDISYHGEYGLRELVAKAADILRDHEVIRERNLVNEFLKHIVKDTGLATYGEREVRKALELGAVDTLLI
SEGYDKVRVHVKCNNCGWEELKTMSEEEYEAYKKRIQTCPKCGSQNLTFEKWEVAEELIKIAEEAGSNVEIISLDTEEGQ
QFYRAFGGLGAILRFRIQGV
>Mature_420_residues
MRVMTSRDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTAQNIKSKTTRKNVLGALERAMQHLK
LYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLNVRLYRCDQTFVTEPLEEMLRVKEAYGLITVEKNEATIGILRG
KRIEVLDELTSNVPGKTRAGGQSARRYERIREQETHEFMKRIGEHANRIFLPLLESGELKGIIVGGPGPTKEEFVEGDYL
HHELKKKIIGIVDISYHGEYGLRELVAKAADILRDHEVIRERNLVNEFLKHIVKDTGLATYGEREVRKALELGAVDTLLI
SEGYDKVRVHVKCNNCGWEELKTMSEEEYEAYKKRIQTCPKCGSQNLTFEKWEVAEELIKIAEEAGSNVEIISLDTEEGQ
QFYRAFGGLGAILRFRIQGV

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family

Homologues:

Organism=Homo sapiens, GI4759034, Length=416, Percent_Identity=33.4134615384615, Blast_Score=250, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI72000368, Length=411, Percent_Identity=33.3333333333333, Blast_Score=235, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6319619, Length=412, Percent_Identity=32.2815533980583, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI28574632, Length=413, Percent_Identity=34.3825665859564, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI21356433, Length=413, Percent_Identity=34.3825665859564, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24667392, Length=413, Percent_Identity=34.3825665859564, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24667388, Length=413, Percent_Identity=34.3825665859564, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24667380, Length=413, Percent_Identity=34.3825665859564, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24667384, Length=413, Percent_Identity=34.3825665859564, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF1_PYRFU (Q8U0J4)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579322.1
- ProteinModelPortal:   Q8U0J4
- SMR:   Q8U0J4
- EnsemblBacteria:   EBPYRT00000005129
- GeneID:   1469469
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1593
- NMPDR:   fig|186497.1.peg.1640
- GeneTree:   EBGT00050000022711
- HOGENOM:   HBG497188
- OMA:   GDYLHHE
- ProtClustDB:   PRK04011
- GO:   GO:0005737
- HAMAP:   MF_00424
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403
- PANTHER:   PTHR10113
- PANTHER:   PTHR10113:SF2
- TIGRFAMs:   TIGR03676

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: NA

Molecular weight: Translated: 47955; Mature: 47955

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMTSRDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTAQNIK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SKTTRKNVLGALERAMQHLKLYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLNVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCEEEEEEEECCCCCCEE
LYRCDQTFVTEPLEEMLRVKEAYGLITVEKNEATIGILRGKRIEVLDELTSNVPGKTRAG
EEECCHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCC
GQSARRYERIREQETHEFMKRIGEHANRIFLPLLESGELKGIIVGGPGPTKEEFVEGDYL
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCHHHHHCCHHH
HHELKKKIIGIVDISYHGEYGLRELVAKAADILRDHEVIRERNLVNEFLKHIVKDTGLAT
HHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YGEREVRKALELGAVDTLLISEGYDKVRVHVKCNNCGWEELKTMSEEEYEAYKKRIQTCP
CCHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCC
KCGSQNLTFEKWEVAEELIKIAEEAGSNVEIISLDTEEGQQFYRAFGGLGAILRFRIQGV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVMTSRDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTAQNIK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
SKTTRKNVLGALERAMQHLKLYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLNVR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHCCCCEEEEEEEECCCCCCEE
LYRCDQTFVTEPLEEMLRVKEAYGLITVEKNEATIGILRGKRIEVLDELTSNVPGKTRAG
EEECCHHHHHHHHHHHHHHHHHHCEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCC
GQSARRYERIREQETHEFMKRIGEHANRIFLPLLESGELKGIIVGGPGPTKEEFVEGDYL
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCHHHHHCCHHH
HHELKKKIIGIVDISYHGEYGLRELVAKAADILRDHEVIRERNLVNEFLKHIVKDTGLAT
HHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YGEREVRKALELGAVDTLLISEGYDKVRVHVKCNNCGWEELKTMSEEEYEAYKKRIQTCP
CCHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCC
KCGSQNLTFEKWEVAEELIKIAEEAGSNVEIISLDTEEGQQFYRAFGGLGAILRFRIQGV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA