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The map label for this gene is trpB2

Identifier: 18977964

GI number: 18977964

Start: 1486031

End: 1487371

Strand: Direct

Name: trpB2

Synonym: PF1592

Alternate gene names: 18977964

Gene position: 1486031-1487371 (Clockwise)

Preceding gene: 18977963

Following gene: 18977966

Centisome position: 77.87

GC content: 42.21

Gene sequence:

>1341_bases
ATGAAGGTAGTTCTTCCAGACGGTAGAATACCTAGGAGATGGTATAATATACTCCCCGATTTACCGGAACCCTTAGCTCC
CCCATTGGATCCAGAGACAAATGAACCCGTAGACCCCAAGAAGTTGGAGAGGATATTTGCGAAAGAACTAGTTAAGCAGG
AAATGAGCACAAAGAGATACATAAAAATTCCCGAAGAAGTTAGAAAGATGTATTCTAAAATAGGGAGGCCTACACCTCTT
TTCAGAGCTACAAACCTCGAAAAATACCTAAATACTCCTGCAAGGATTTACTTCAAGTTTGAGGGAGCAACAGTTACAGG
AAGCCACAAAATCAATACTGCCTTAGCTCAGGCATATTATGCAAAGAAGGAAGGAATAGAGAGGCTAGTTACTGAAACTG
GAGCTGGTCAATGGGGAACTGCATTGAGCTTGGCTGGAGCCTTGATGGGCATTAAGGTTAGAGTATATATGGCAAGAGCA
AGTTACGAGCAGAAGCCATATAGAAAAGTTTTAATGAGGATTTATGGAGCAGAGGTATTCCCAAGTCCCAGCGAGAATAC
TGAAATAGGAAAAAGATTCCTAAGTGAAAATCCAAACCACCCAGGAAGCTTAGGTATTGCAATTAGTGAGGCAATAGAAG
ATGTACTAAAAGATGAAAAGGCAAGATACTCCCTAGGAAGTGTTCTAAACCACGTACTAATGCATCAAACGGTAATTGGA
CTTGAAGCAAAGCAGCAAATGGAAGAGTTTGAGGAGCCCGATGTTATAATAGGATGTGTTGGTGGAGGAAGCAACTTTGC
CGGTCTTGCTTATCCCTTTGTAAAAGAAGTCCTTGACGGAGACAATGAATACGAATTTATAGCAGTAGAGCCAAAAGCTG
CTCCTTCAATGACTAGAGGAGTCTACACTTACGATTTTGGGGATTCTGGAGAGCTAACCCCTAAGTTAAAGATGCATACT
CTTGGGCATAGATACCATGTTCCCCCCATACATGCTGGAGGATTAAGATATCACGGAGTTGCACCTACTTTAAGTGTACT
CGTTAACAATGGAATCGTTAAGCCAATAGCTTACCATCAAACAGAAGTTTTTGAAGCTGCAGCGTTATTTGCTAAGCTTG
AAGGTATTGTTCCAGCTCCAGAAAGTGCCCATGCAATAAAGGCCACAATAGACAAAGCCATCGAGGCTAAGAGAGAAGGA
AAAGAAATTGTCATCTTGTTCAACTTAAGCGGCCACGGCCTACTAGATCTCCATGGATACGAAGAATACTTGGAGGGAAG
GCTCCAAGACTACGAGCCTAAAGATCTTCCCATATCAAACCCCTTGAATCCTAAACCTTAG

Upstream 100 bases:

>100_bases
CTCTCCTTTTGGTTATTTCTTGATGTCTATGGGTGGTCGTTTTTGGTAGAATCGTGAACTTGTTTCAACTGCATGCCTCC
AATTCTAGGAGGGAGTTTAG

Downstream 100 bases:

>100_bases
GATTGCCCCAAGACCTCCAAAGGCCCTATAAAACTGTTGGCCTTCCTCAGTGTCGAGAGATATAATTTCAACATTTGACC
CTGCTTCCTCTGCTATTTTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKVVLPDGRIPRRWYNILPDLPEPLAPPLDPETNEPVDPKKLERIFAKELVKQEMSTKRYIKIPEEVRKMYSKIGRPTPL
FRATNLEKYLNTPARIYFKFEGATVTGSHKINTALAQAYYAKKEGIERLVTETGAGQWGTALSLAGALMGIKVRVYMARA
SYEQKPYRKVLMRIYGAEVFPSPSENTEIGKRFLSENPNHPGSLGIAISEAIEDVLKDEKARYSLGSVLNHVLMHQTVIG
LEAKQQMEEFEEPDVIIGCVGGGSNFAGLAYPFVKEVLDGDNEYEFIAVEPKAAPSMTRGVYTYDFGDSGELTPKLKMHT
LGHRYHVPPIHAGGLRYHGVAPTLSVLVNNGIVKPIAYHQTEVFEAAALFAKLEGIVPAPESAHAIKATIDKAIEAKREG
KEIVILFNLSGHGLLDLHGYEEYLEGRLQDYEPKDLPISNPLNPKP

Sequences:

>Translated_446_residues
MKVVLPDGRIPRRWYNILPDLPEPLAPPLDPETNEPVDPKKLERIFAKELVKQEMSTKRYIKIPEEVRKMYSKIGRPTPL
FRATNLEKYLNTPARIYFKFEGATVTGSHKINTALAQAYYAKKEGIERLVTETGAGQWGTALSLAGALMGIKVRVYMARA
SYEQKPYRKVLMRIYGAEVFPSPSENTEIGKRFLSENPNHPGSLGIAISEAIEDVLKDEKARYSLGSVLNHVLMHQTVIG
LEAKQQMEEFEEPDVIIGCVGGGSNFAGLAYPFVKEVLDGDNEYEFIAVEPKAAPSMTRGVYTYDFGDSGELTPKLKMHT
LGHRYHVPPIHAGGLRYHGVAPTLSVLVNNGIVKPIAYHQTEVFEAAALFAKLEGIVPAPESAHAIKATIDKAIEAKREG
KEIVILFNLSGHGLLDLHGYEEYLEGRLQDYEPKDLPISNPLNPKP
>Mature_446_residues
MKVVLPDGRIPRRWYNILPDLPEPLAPPLDPETNEPVDPKKLERIFAKELVKQEMSTKRYIKIPEEVRKMYSKIGRPTPL
FRATNLEKYLNTPARIYFKFEGATVTGSHKINTALAQAYYAKKEGIERLVTETGAGQWGTALSLAGALMGIKVRVYMARA
SYEQKPYRKVLMRIYGAEVFPSPSENTEIGKRFLSENPNHPGSLGIAISEAIEDVLKDEKARYSLGSVLNHVLMHQTVIG
LEAKQQMEEFEEPDVIIGCVGGGSNFAGLAYPFVKEVLDGDNEYEFIAVEPKAAPSMTRGVYTYDFGDSGELTPKLKMHT
LGHRYHVPPIHAGGLRYHGVAPTLSVLVNNGIVKPIAYHQTEVFEAAALFAKLEGIVPAPESAHAIKATIDKAIEAKREG
KEIVILFNLSGHGLLDLHGYEEYLEGRLQDYEPKDLPISNPLNPKP

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=377, Percent_Identity=32.6259946949602, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=380, Percent_Identity=32.3684210526316, Blast_Score=147, Evalue=4e-36,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB2_PYRFU (Q8U0J5)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579321.1
- ProteinModelPortal:   Q8U0J5
- EnsemblBacteria:   EBPYRT00000005389
- GeneID:   1469468
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1592
- NMPDR:   fig|186497.1.peg.1639
- GeneTree:   EBGT00050000022951
- HOGENOM:   HBG303148
- OMA:   YTLGHDF
- ProtClustDB:   PRK12391
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026
- TIGRFAMs:   TIGR01415

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 49617; Mature: 49617

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLPDGRIPRRWYNILPDLPEPLAPPLDPETNEPVDPKKLERIFAKELVKQEMSTKRY
CEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHE
IKIPEEVRKMYSKIGRPTPLFRATNLEKYLNTPARIYFKFEGATVTGSHKINTALAQAYY
EECCHHHHHHHHHCCCCCCCHHHCCHHHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHH
AKKEGIERLVTETGAGQWGTALSLAGALMGIKVRVYMARASYEQKPYRKVLMRIYGAEVF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEHHCCCCCHHHHHHHHHHCCCCC
PSPSENTEIGKRFLSENPNHPGSLGIAISEAIEDVLKDEKARYSLGSVLNHVLMHQTVIG
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAKQQMEEFEEPDVIIGCVGGGSNFAGLAYPFVKEVLDGDNEYEFIAVEPKAAPSMTRG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCC
VYTYDFGDSGELTPKLKMHTLGHRYHVPPIHAGGLRYHGVAPTLSVLVNNGIVKPIAYHQ
EEEEECCCCCCCCCCEEEEECCCEECCCCCCCCCEEECCCCHHHHHHHCCCCCCCHHHHH
TEVFEAAALFAKLEGIVPAPESAHAIKATIDKAIEAKREGKEIVILFNLSGHGLLDLHGY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCH
EEYLEGRLQDYEPKDLPISNPLNPKP
HHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVVLPDGRIPRRWYNILPDLPEPLAPPLDPETNEPVDPKKLERIFAKELVKQEMSTKRY
CEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHE
IKIPEEVRKMYSKIGRPTPLFRATNLEKYLNTPARIYFKFEGATVTGSHKINTALAQAYY
EECCHHHHHHHHHCCCCCCCHHHCCHHHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHH
AKKEGIERLVTETGAGQWGTALSLAGALMGIKVRVYMARASYEQKPYRKVLMRIYGAEVF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEHHCCCCCHHHHHHHHHHCCCCC
PSPSENTEIGKRFLSENPNHPGSLGIAISEAIEDVLKDEKARYSLGSVLNHVLMHQTVIG
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAKQQMEEFEEPDVIIGCVGGGSNFAGLAYPFVKEVLDGDNEYEFIAVEPKAAPSMTRG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCC
VYTYDFGDSGELTPKLKMHTLGHRYHVPPIHAGGLRYHGVAPTLSVLVNNGIVKPIAYHQ
EEEEECCCCCCCCCCEEEEECCCEECCCCCCCCCEEECCCCHHHHHHHCCCCCCCHHHHH
TEVFEAAALFAKLEGIVPAPESAHAIKATIDKAIEAKREGKEIVILFNLSGHGLLDLHGY
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCH
EEYLEGRLQDYEPKDLPISNPLNPKP
HHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA