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The map label for this gene is rtcA

Identifier: 18977921

GI number: 18977921

Start: 1446828

End: 1447856

Strand: Direct

Name: rtcA

Synonym: PF1549

Alternate gene names: 18977921

Gene position: 1446828-1447856 (Clockwise)

Preceding gene: 18977920

Following gene: 18977922

Centisome position: 75.82

GC content: 43.34

Gene sequence:

>1029_bases
ATGATAATAATAGACGGAAGTTATGGCGAGGGTGGAGGACAGATCTTGAGAACCTCCATAGCACTCTCAGCCATAACTGG
AGAACCCGTGAAGATTATAAACATTCGAGCAAACAGACCAAACCCTGGACTTAGACCACAACATCTAAACGCAATTTTAG
CTTTGAAAAAACTAGCAAATGCTAAAGTCGAAGGAGCTGAAGTAGGGTCTAGAGAAGTAACTTTCATCCCAGGGGAGCTA
AAAGGAGGGGAAATAAGGGTAGACATAGGAACTGCGGGTAGCATAACCCTTGTTCTCCAGGCCCTCCTTCCCGCAATGGT
ATTTGCAAAAGATACTGTAGAATTCAAAATAACAGGGGGAACAGACGTCTCCTGGAGTCCACCTGTGGATTATCTTATAA
ATGTCACAATGTTCGCCCTAAGGAAAATTGGAATTGAGGGAGAGATAAAACTTCTTAGAAGGGGACACTATCCAAAAGGA
GGAGGGATAGTAGCGGGTTACGTGAAACCCTGGATAGAGAGAAAGGAGCTAATAGCGGAAGAGTTCGAAAATATATACAA
GGTCTCGGGAATAAGTCATGCAACTAATTTACCAGCCCATGTAGCTGAGAGACAGGCAAAAGCCGCCATGGAAGAACTAA
AGGTTCTTGGAGTTCCAATTGAAATAAAAAAGGAGGTTTCACACTCCTTAGGCCCAGGAAGTGGAATAGTTGTATGGGCT
GAAACCGAGTGCCTAAGATTAGGTGGAGATGCTCTAGGCAAAAAGGGGAAACCAGCCGAAGAAGTAGGAAGGGAAGCAGC
CCAAGAGCTTTTATCCCAGGTAAAAACCAAGGCCTGTGTGGATAAGTTCCTTGGAGACCAAATAATTCCCTTCCTAGCAA
TAAGTGGAGGAAAGATTAAGGTTGCAGAGATAACAAAGCATTTAATTACAAATGTTTGGGTTGTAGAGCAATTCTTTGGT
AAAGTTTTTGAAGTTAAGGGAGGAGTTGGTGAAAAGGGAGAAGTTAGAGTAGTTAGGAAAGCCTGGTAG

Upstream 100 bases:

>100_bases
TAATAAAAAACTTATTTGAAAAATTAAAACTCACCGAGAAGGGCAAAGATGAAGTATCACTTTTAAAGGAAGCTGCTACT
ATCATCACTGGGGGCACGCC

Downstream 100 bases:

>100_bases
GGTGAGGAAAAATGAAAGTCGGAATAATGAGTGATACTCACGACAATTTGGAAGCAATAAAGCTTGCCGTGGAGTACTTC
AATGAAGAGGGGGTTGATTT

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIIIDGSYGEGGGQILRTSIALSAITGEPVKIINIRANRPNPGLRPQHLNAILALKKLANAKVEGAEVGSREVTFIPGEL
KGGEIRVDIGTAGSITLVLQALLPAMVFAKDTVEFKITGGTDVSWSPPVDYLINVTMFALRKIGIEGEIKLLRRGHYPKG
GGIVAGYVKPWIERKELIAEEFENIYKVSGISHATNLPAHVAERQAKAAMEELKVLGVPIEIKKEVSHSLGPGSGIVVWA
ETECLRLGGDALGKKGKPAEEVGREAAQELLSQVKTKACVDKFLGDQIIPFLAISGGKIKVAEITKHLITNVWVVEQFFG
KVFEVKGGVGEKGEVRVVRKAW

Sequences:

>Translated_342_residues
MIIIDGSYGEGGGQILRTSIALSAITGEPVKIINIRANRPNPGLRPQHLNAILALKKLANAKVEGAEVGSREVTFIPGEL
KGGEIRVDIGTAGSITLVLQALLPAMVFAKDTVEFKITGGTDVSWSPPVDYLINVTMFALRKIGIEGEIKLLRRGHYPKG
GGIVAGYVKPWIERKELIAEEFENIYKVSGISHATNLPAHVAERQAKAAMEELKVLGVPIEIKKEVSHSLGPGSGIVVWA
ETECLRLGGDALGKKGKPAEEVGREAAQELLSQVKTKACVDKFLGDQIIPFLAISGGKIKVAEITKHLITNVWVVEQFFG
KVFEVKGGVGEKGEVRVVRKAW
>Mature_342_residues
MIIIDGSYGEGGGQILRTSIALSAITGEPVKIINIRANRPNPGLRPQHLNAILALKKLANAKVEGAEVGSREVTFIPGEL
KGGEIRVDIGTAGSITLVLQALLPAMVFAKDTVEFKITGGTDVSWSPPVDYLINVTMFALRKIGIEGEIKLLRRGHYPKG
GGIVAGYVKPWIERKELIAEEFENIYKVSGISHATNLPAHVAERQAKAAMEELKVLGVPIEIKKEVSHSLGPGSGIVVWA
ETECLRLGGDALGKKGKPAEEVGREAAQELLSQVKTKACVDKFLGDQIIPFLAISGGKIKVAEITKHLITNVWVVEQFFG
KVFEVKGGVGEKGEVRVVRKAW

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=333, Percent_Identity=38.7387387387387, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI195927044, Length=346, Percent_Identity=37.2832369942197, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI157426877, Length=339, Percent_Identity=26.2536873156342, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI48994937, Length=330, Percent_Identity=40, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI32564710, Length=286, Percent_Identity=29.3706293706294, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324564, Length=312, Percent_Identity=23.3974358974359, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI18543269, Length=334, Percent_Identity=35.9281437125749, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI18860065, Length=340, Percent_Identity=27.6470588235294, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_PYRFU (Q8U0N7)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579278.1
- ProteinModelPortal:   Q8U0N7
- SMR:   Q8U0N7
- EnsemblBacteria:   EBPYRT00000006026
- GeneID:   1469425
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1549
- NMPDR:   fig|186497.1.peg.1596
- GeneTree:   EBGT00050000022634
- HOGENOM:   HBG683539
- OMA:   GSTPLLF
- ProtClustDB:   PRK04204
- BRENDA:   6.5.1.4
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR000228
- InterPro:   IPR020719
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 36805; Mature: 36805

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 307-307 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIDGSYGEGGGQILRTSIALSAITGEPVKIINIRANRPNPGLRPQHLNAILALKKLAN
CEEEECCCCCCCCHHHHHHHEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHC
AKVEGAEVGSREVTFIPGELKGGEIRVDIGTAGSITLVLQALLPAMVFAKDTVEFKITGG
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
TDVSWSPPVDYLINVTMFALRKIGIEGEIKLLRRGHYPKGGGIVAGYVKPWIERKELIAE
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCEEEHHHHHHHHHHHHHHH
EFENIYKVSGISHATNLPAHVAERQAKAAMEELKVLGVPIEIKKEVSHSLGPGSGIVVWA
HHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCCEEEEE
ETECLRLGGDALGKKGKPAEEVGREAAQELLSQVKTKACVDKFLGDQIIPFLAISGGKIK
CCHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCEEE
VAEITKHLITNVWVVEQFFGKVFEVKGGVGEKGEVRVVRKAW
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIIIDGSYGEGGGQILRTSIALSAITGEPVKIINIRANRPNPGLRPQHLNAILALKKLAN
CEEEECCCCCCCCHHHHHHHEEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHC
AKVEGAEVGSREVTFIPGELKGGEIRVDIGTAGSITLVLQALLPAMVFAKDTVEFKITGG
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
TDVSWSPPVDYLINVTMFALRKIGIEGEIKLLRRGHYPKGGGIVAGYVKPWIERKELIAE
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCEEEHHHHHHHHHHHHHHH
EFENIYKVSGISHATNLPAHVAERQAKAAMEELKVLGVPIEIKKEVSHSLGPGSGIVVWA
HHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCCEEEEE
ETECLRLGGDALGKKGKPAEEVGREAAQELLSQVKTKACVDKFLGDQIIPFLAISGGKIK
CCHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCEEE
VAEITKHLITNVWVVEQFFGKVFEVKGGVGEKGEVRVVRKAW
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA