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The map label for this gene is gcvH [H]

Identifier: 18977864

GI number: 18977864

Start: 1392151

End: 1392555

Strand: Reverse

Name: gcvH [H]

Synonym: PF1492

Alternate gene names: 18977864

Gene position: 1392555-1392151 (Counterclockwise)

Preceding gene: 18977867

Following gene: 18977862

Centisome position: 72.98

GC content: 40.0

Gene sequence:

>405_bases
ATGATTGAAGTTGGAGAATACAAAATTAAGGAAGGTTTGTACTATACAAAGGAGCATGAATGGGTTCAGGTCTTAGAAGA
CGGAACTGTTTTAGTTGGTATAACTGACTATGCTCAAAAAGAGTTAGGAGACCTGGCCTATGTTGAACTTCCCGAAGTGG
GAAAGGTCGTAGAGAAGGGAGATGTCCTTTGTGAAGTTGAGAGCGTTAAAGCCGTTAGTGAAGTCTATGCTCCCGTAAGT
GGGGAAGTAATAGAAGTGAATGAAGAACTTGCAGATAGTCCAGAGAAAATAAATGAAGATCCCTATGAAGCTTGGATAGC
CAAGATTAAGCCAAAGAACTTAGAAGAAGAGCTTAAAGAGTTAATGGATGCAAAAGCCTACGCAGAATACTTAAAGACCC
TCTAA

Upstream 100 bases:

>100_bases
CTTTGGCATTTATCTCTTTGATCCATAACACCTTCATGTTCAATTTTTGGGAGACAATGATTTTAAAGGGTATTTAATGA
GATTAAAAGTGGTGGTTTCC

Downstream 100 bases:

>100_bases
AGTATCTTTCCTTCCATTTTCATTCTTACCAGCTTTGTGACGTAACCTGCTATCCTGTTTCTTATCTTTTTGCTTGTTAC
GTTAGTTAATTCCTGAACTT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MIEVGEYKIKEGLYYTKEHEWVQVLEDGTVLVGITDYAQKELGDLAYVELPEVGKVVEKGDVLCEVESVKAVSEVYAPVS
GEVIEVNEELADSPEKINEDPYEAWIAKIKPKNLEEELKELMDAKAYAEYLKTL

Sequences:

>Translated_134_residues
MIEVGEYKIKEGLYYTKEHEWVQVLEDGTVLVGITDYAQKELGDLAYVELPEVGKVVEKGDVLCEVESVKAVSEVYAPVS
GEVIEVNEELADSPEKINEDPYEAWIAKIKPKNLEEELKELMDAKAYAEYLKTL
>Mature_134_residues
MIEVGEYKIKEGLYYTKEHEWVQVLEDGTVLVGITDYAQKELGDLAYVELPEVGKVVEKGDVLCEVESVKAVSEVYAPVS
GEVIEVNEELADSPEKINEDPYEAWIAKIKPKNLEEELKELMDAKAYAEYLKTL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=47.1074380165289, Blast_Score=107, Evalue=3e-24,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=46.2809917355372, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789271, Length=116, Percent_Identity=55.1724137931034, Blast_Score=125, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=122, Percent_Identity=45.0819672131148, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=45, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=101, Percent_Identity=47.5247524752475, Blast_Score=103, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=45, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 15142; Mature: 15142

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEVGEYKIKEGLYYTKEHEWVQVLEDGTVLVGITDYAQKELGDLAYVELPEVGKVVEKG
CCCCCCEECCCCEEECCCHHHHHHCCCCEEEEEEHHHHHHHHCCEEEEECCHHHHHHHCC
DVLCEVESVKAVSEVYAPVSGEVIEVNEELADSPEKINEDPYEAWIAKIKPKNLEEELKE
CEEEEHHHHHHHHHHHCCCCCCEEEECHHHCCCCHHCCCCHHHHHHHHCCCCCHHHHHHH
LMDAKAYAEYLKTL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIEVGEYKIKEGLYYTKEHEWVQVLEDGTVLVGITDYAQKELGDLAYVELPEVGKVVEKG
CCCCCCEECCCCEEECCCHHHHHHCCCCEEEEEEHHHHHHHHCCEEEEECCHHHHHHHCC
DVLCEVESVKAVSEVYAPVSGEVIEVNEELADSPEKINEDPYEAWIAKIKPKNLEEELKE
CEEEEHHHHHHHHHHHCCCCCCEEEECHHHCCCCHHCCCCHHHHHHHHCCCCCHHHHHHH
LMDAKAYAEYLKTL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]