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The map label for this gene is ytcB [H]

Identifier: 18977729

GI number: 18977729

Start: 1275204

End: 1276214

Strand: Direct

Name: ytcB [H]

Synonym: PF1357

Alternate gene names: 18977729

Gene position: 1275204-1276214 (Clockwise)

Preceding gene: 18977728

Following gene: 33359561

Centisome position: 66.83

GC content: 40.45

Gene sequence:

>1011_bases
ATGATGGACCCGATAAAGTCAGATGTAAGAGAAATTGTAAGAGCATTAGATAGTGCTAATTTTGAGGGTAAGACTGCTCT
GGTTACAGGTGGTGCTGGTTTTTTGGGATCTTGGCTTTGTGATGTGTTAATCGAGTTGGGAGCAAAGGTTTATTGTGTTG
ATAACTTTGCCAGTGGTCGTTGGGAAAACATCTCTCATCTAACAAGTGAGGAGAATTTTGTATTTATAGAGCACGATGTT
TCGAAACCCTTGGAAATCAGAGAAAAACTTGATTTTATCTTTCATTTCGCCTCAAGGGCATCACCTTTTGAATTCGAGCA
CTATCCTCTAGAGATAATAGATGCAAACACCCTTGGAACTAGGAATATGCTTGAATTGGCTAAAAAGAATAATGCGAGAT
TTATCTTTGCCTCAACGAGCGAAATTTATGGTCATCCTGAAGTTGTCCCAACTCCTGAAACGTATTGGGGTTACGTGAAT
CCAATTGGCATTAGAAGTTGCTATGATGAGTCAAAGAGACTTGGAGAGGCTCTCACGATGGCTTATTATAGGCAGTTCAA
TGTTGATGTGAGAATAGTTAGGATTTTTAATACGTATGGGCCAAGAATGCGAGCTGATGGAGTTTATGGTAGGGTAGTCC
CAAGGTTTATAAGTCAAGCCTTGAATGAAGAGCCAATTACTGTTTTTGGAGATGGGAGCCAGACAAGGAGTTTTTGTTAT
GTAACTGATCTCATAACGGGAGTATTGAAGTTTGCCGCTGTGGAGAACGGTAGAGGGGAAGTTGTGAATTTGGGCAATCC
CCGGGAGATTAGTATTCTTGAGCTTGCTTATCTCATAAAGAAGCTTACTAATTCTGATTCGCCAATAGAATTTCATCCAC
TACCTCCTGATGATCCTCCTAGGAGGTGTCCAGACATAAGTAAAGCACAAAAACTGCTAAACTGGAAACCAAAGGTCGAA
CTCGAAGAAGGGCTCAAAAAGACAATAAAATGGTTTGGGGGGAAAATTTAA

Upstream 100 bases:

>100_bases
TTTTTGATTGGCTACGGGCAAATATTGAATTAGGATTAGAAGATGAAGAACTTAGTGAAAAGCTAAGAGAACTTATCAAA
TCGTTAGTGGGGGAAAGAAT

Downstream 100 bases:

>100_bases
TTGTCTGAAGTTATTTATACAATAGGAGCTGTTCTTTTTGGGGGAGGGATAGGGAACACTGCATATTATGAAGTAGAAGG
ATTGCATCGAAATGGGAGCT

Product: UDP- or dTTP-glucose 4-epimerase or 4-6-dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MMDPIKSDVREIVRALDSANFEGKTALVTGGAGFLGSWLCDVLIELGAKVYCVDNFASGRWENISHLTSEENFVFIEHDV
SKPLEIREKLDFIFHFASRASPFEFEHYPLEIIDANTLGTRNMLELAKKNNARFIFASTSEIYGHPEVVPTPETYWGYVN
PIGIRSCYDESKRLGEALTMAYYRQFNVDVRIVRIFNTYGPRMRADGVYGRVVPRFISQALNEEPITVFGDGSQTRSFCY
VTDLITGVLKFAAVENGRGEVVNLGNPREISILELAYLIKKLTNSDSPIEFHPLPPDDPPRRCPDISKAQKLLNWKPKVE
LEEGLKKTIKWFGGKI

Sequences:

>Translated_336_residues
MMDPIKSDVREIVRALDSANFEGKTALVTGGAGFLGSWLCDVLIELGAKVYCVDNFASGRWENISHLTSEENFVFIEHDV
SKPLEIREKLDFIFHFASRASPFEFEHYPLEIIDANTLGTRNMLELAKKNNARFIFASTSEIYGHPEVVPTPETYWGYVN
PIGIRSCYDESKRLGEALTMAYYRQFNVDVRIVRIFNTYGPRMRADGVYGRVVPRFISQALNEEPITVFGDGSQTRSFCY
VTDLITGVLKFAAVENGRGEVVNLGNPREISILELAYLIKKLTNSDSPIEFHPLPPDDPPRRCPDISKAQKLLNWKPKVE
LEEGLKKTIKWFGGKI
>Mature_336_residues
MMDPIKSDVREIVRALDSANFEGKTALVTGGAGFLGSWLCDVLIELGAKVYCVDNFASGRWENISHLTSEENFVFIEHDV
SKPLEIREKLDFIFHFASRASPFEFEHYPLEIIDANTLGTRNMLELAKKNNARFIFASTSEIYGHPEVVPTPETYWGYVN
PIGIRSCYDESKRLGEALTMAYYRQFNVDVRIVRIFNTYGPRMRADGVYGRVVPRFISQALNEEPITVFGDGSQTRSFCY
VTDLITGVLKFAAVENGRGEVVNLGNPREISILELAYLIKKLTNSDSPIEFHPLPPDDPPRRCPDISKAQKLLNWKPKVE
LEEGLKKTIKWFGGKI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=48.8235294117647, Blast_Score=319, Evalue=2e-87,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=30.7692307692308, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI56237023, Length=339, Percent_Identity=23.8938053097345, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56118217, Length=339, Percent_Identity=23.8938053097345, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI189083684, Length=339, Percent_Identity=23.8938053097345, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI48994969, Length=335, Percent_Identity=27.1641791044776, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788353, Length=336, Percent_Identity=27.6785714285714, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786974, Length=330, Percent_Identity=27.5757575757576, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788589, Length=341, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788365, Length=264, Percent_Identity=26.8939393939394, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790049, Length=321, Percent_Identity=25.8566978193146, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=314, Percent_Identity=47.7707006369427, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71982035, Length=341, Percent_Identity=26.9794721407625, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568069, Length=352, Percent_Identity=26.1363636363636, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=343, Percent_Identity=26.8221574344023, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=26.099706744868, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319493, Length=344, Percent_Identity=27.3255813953488, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356223, Length=313, Percent_Identity=51.7571884984025, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI19923002, Length=340, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 38054; Mature: 38054

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDPIKSDVREIVRALDSANFEGKTALVTGGAGFLGSWLCDVLIELGAKVYCVDNFASGR
CCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
WENISHLTSEENFVFIEHDVSKPLEIREKLDFIFHFASRASPFEFEHYPLEIIDANTLGT
CCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
RNMLELAKKNNARFIFASTSEIYGHPEVVPTPETYWGYVNPIGIRSCYDESKRLGEALTM
HHHHHHHHCCCCEEEEEECHHHCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHH
AYYRQFNVDVRIVRIFNTYGPRMRADGVYGRVVPRFISQALNEEPITVFGDGSQTRSFCY
HHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
VTDLITGVLKFAAVENGRGEVVNLGNPREISILELAYLIKKLTNSDSPIEFHPLPPDDPP
HHHHHHHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
RRCPDISKAQKLLNWKPKVELEEGLKKTIKWFGGKI
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMDPIKSDVREIVRALDSANFEGKTALVTGGAGFLGSWLCDVLIELGAKVYCVDNFASGR
CCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
WENISHLTSEENFVFIEHDVSKPLEIREKLDFIFHFASRASPFEFEHYPLEIIDANTLGT
CCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCC
RNMLELAKKNNARFIFASTSEIYGHPEVVPTPETYWGYVNPIGIRSCYDESKRLGEALTM
HHHHHHHHCCCCEEEEEECHHHCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHH
AYYRQFNVDVRIVRIFNTYGPRMRADGVYGRVVPRFISQALNEEPITVFGDGSQTRSFCY
HHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
VTDLITGVLKFAAVENGRGEVVNLGNPREISILELAYLIKKLTNSDSPIEFHPLPPDDPP
HHHHHHHHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
RRCPDISKAQKLLNWKPKVELEEGLKKTIKWFGGKI
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]