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The map label for this gene is thiD [C]

Identifier: 18977705

GI number: 18977705

Start: 1253839

End: 1255194

Strand: Reverse

Name: thiD [C]

Synonym: PF1333

Alternate gene names: 18977705

Gene position: 1255194-1253839 (Counterclockwise)

Preceding gene: 18977706

Following gene: 18977700

Centisome position: 65.78

GC content: 46.31

Gene sequence:

>1356_bases
ATGAAAAGGTTAGTGAAGACTGCCCTCACAATTGCTGGGAGCGATAGTGGTGGAGGAGCGGGAATTGAGGCGGATCTAAA
AACCTTTACAGCCTTTGGAGTCCACGGCCTAGTTGCCATAACCTCAGTTACTGCCCAAAACACCCAAGCAGTTACGGCAA
TCCACGATATTCCTCCAGAAGTTGTGGCAGAGCAGATAAGGGCCGTGGCCGAAGATATAGGGGTTGATGCCGCAAAGACA
GGAATGCTAAGCAATGCTGAGATAATTAAAGCAGTGGCAAAGACCGTGAAAAACTACGACTTTCCACTCGTAGTTGATCC
AGTAATGATAGCTAAGAGCGGAGCTCCCCTCCTTAGAGAGGACGCGGTAGATGCTTTAATCAAGTACATTCTCCCCTTGG
CAACGCTTGTAACCCCAAACAGGTTCGAGGCAGAAAAGCTGAGCGGGATGAAAATTAGAAGCGTTGAGGATGCAAAGATA
GTTGCGAAGAAAATCGCAGAGGAAACTGGAGCAGAGGCAGTGATAGTTAAGGGAGGACACATTGAGGGAGAGGAGGCAGT
TGACGTTCTCTACCACAATGGAACTTTTAGGGAGTATAGAGCCCCAAAAGTTGAAGGTTGCACTCACGGTACTGGATGTA
GCTTCTCCGCTGCCATAACAGCAAACCTAGCGAAAGGTTTGGGATTAGAAGAGGCAATAGAAGTTGCGAAGAAGTTCATA
ACCTTGGGCATAGCTATGGGCCACAGGATAGGACATGGTCATTGCCCAGTGAATCAATCAGCTTGGATAGAGATTCCTGC
AGAGAAGTGGAGAATTTATGAAGAGTTAACGAATGCAGTGAGAGAATTCGAAAGCATTAATCCAGTAAGGCTAATTCCAG
AGGTGGGAACTAACTTCGTCTACTCACTTCCACTTCCCTACGCCAGAAGCACTAAAGACGTTGCGGGGGTTAAGGGGAGA
ATCGTCAAGTACGGAAACTCAGTAAAGGCCGTGGGCCCTGTTGAGTTTGGAGCAAGCGACCACCTAGCTAGGGCTGTCCT
CACTTACATGAGATTCTACCCAGAGTACAGGAGTGCCATAAACATTAGATACTCCAGAGAAATAATAGAAGAAATAATAG
AGATAGCCCAGGAGAGGGGATTCAAAGTTTCATTCTACGATAGAAGAGAGGAACCAGAGGAGATAAAGGCCAAAGAAGGA
GCAACGATACCTTGGGGAATAGAGACCGCGATAAAGAGGATAAAAGAAAGGCCCGACATCATCTATCACCTAGGGGATGT
AGGAAAGGAGCCAATGATATTGGTGTTTGGTAGAAATCCAAGGGAAGTCCTTGAAAAAATAAAGATGTTAATTTAA

Upstream 100 bases:

>100_bases
CTGGAGTTGACGGAATAGCCGTAATATCCGCTGTTATGGGTGCTGAAGATGTTAGAAAGGCTACAGAGGAGTTAAGAAAA
ATCGTAGAGGAGGTTTTAGG

Downstream 100 bases:

>100_bases
AGCTTGACGAAGTGCACTGAACAGGAGATACACGGGTCAAAGGCCCTTACAATTACCTCAAGTCTCTTCTTCATATTTTC
TTCAGACTCAGAGCCATAAA

Product: phosphomethylpyrimidine kinase

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPEVVAEQIRAVAEDIGVDAAKT
GMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIKYILPLATLVTPNRFEAEKLSGMKIRSVEDAKI
VAKKIAEETGAEAVIVKGGHIEGEEAVDVLYHNGTFREYRAPKVEGCTHGTGCSFSAAITANLAKGLGLEEAIEVAKKFI
TLGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTNAVREFESINPVRLIPEVGTNFVYSLPLPYARSTKDVAGVKGR
IVKYGNSVKAVGPVEFGASDHLARAVLTYMRFYPEYRSAINIRYSREIIEEIIEIAQERGFKVSFYDRREEPEEIKAKEG
ATIPWGIETAIKRIKERPDIIYHLGDVGKEPMILVFGRNPREVLEKIKMLI

Sequences:

>Translated_451_residues
MKRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPEVVAEQIRAVAEDIGVDAAKT
GMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIKYILPLATLVTPNRFEAEKLSGMKIRSVEDAKI
VAKKIAEETGAEAVIVKGGHIEGEEAVDVLYHNGTFREYRAPKVEGCTHGTGCSFSAAITANLAKGLGLEEAIEVAKKFI
TLGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTNAVREFESINPVRLIPEVGTNFVYSLPLPYARSTKDVAGVKGR
IVKYGNSVKAVGPVEFGASDHLARAVLTYMRFYPEYRSAINIRYSREIIEEIIEIAQERGFKVSFYDRREEPEEIKAKEG
ATIPWGIETAIKRIKERPDIIYHLGDVGKEPMILVFGRNPREVLEKIKMLI
>Mature_451_residues
MKRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPEVVAEQIRAVAEDIGVDAAKT
GMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIKYILPLATLVTPNRFEAEKLSGMKIRSVEDAKI
VAKKIAEETGAEAVIVKGGHIEGEEAVDVLYHNGTFREYRAPKVEGCTHGTGCSFSAAITANLAKGLGLEEAIEVAKKFI
TLGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTNAVREFESINPVRLIPEVGTNFVYSLPLPYARSTKDVAGVKGR
IVKYGNSVKAVGPVEFGASDHLARAVLTYMRFYPEYRSAINIRYSREIIEEIIEIAQERGFKVSFYDRREEPEEIKAKEG
ATIPWGIETAIKRIKERPDIIYHLGDVGKEPMILVFGRNPREVLEKIKMLI

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=267, Percent_Identity=38.9513108614232, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788758, Length=189, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325378, Length=286, Percent_Identity=32.5174825174825, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=279, Percent_Identity=36.9175627240143, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324517, Length=279, Percent_Identity=35.4838709677419, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293 [H]

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin [H]

EC number: 2.7.4.7

Molecular weight: Translated: 49301; Mature: 49301

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPE
CHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCHH
VVAEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRE
HHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEECHHHEECCCCCHHHH
DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRSVEDAKIVAKKIAEETGAEAVIVKGGH
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEEEECCC
IEGEEAVDVLYHNGTFREYRAPKVEGCTHGTGCSFSAAITANLAKGLGLEEAIEVAKKFI
CCCCHHHEEEEECCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
TLGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTNAVREFESINPVRLIPEVGTNFV
HHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCEE
YSLPLPYARSTKDVAGVKGRIVKYGNSVKAVGPVEFGASDHLARAVLTYMRFYPEYRSAI
EECCCCCCCCCCHHHCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHE
NIRYSREIIEEIIEIAQERGFKVSFYDRREEPEEIKAKEGATIPWGIETAIKRIKERPDI
EHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCE
IYHLGDVGKEPMILVFGRNPREVLEKIKMLI
EEEECCCCCCCEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MKRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPE
CHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCHH
VVAEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRE
HHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCCCEEECHHHEECCCCCHHHH
DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRSVEDAKIVAKKIAEETGAEAVIVKGGH
HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEEEECCC
IEGEEAVDVLYHNGTFREYRAPKVEGCTHGTGCSFSAAITANLAKGLGLEEAIEVAKKFI
CCCCHHHEEEEECCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
TLGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTNAVREFESINPVRLIPEVGTNFV
HHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCEE
YSLPLPYARSTKDVAGVKGRIVKYGNSVKAVGPVEFGASDHLARAVLTYMRFYPEYRSAI
EECCCCCCCCCCHHHCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHE
NIRYSREIIEEIIEIAQERGFKVSFYDRREEPEEIKAKEGATIPWGIETAIKRIKERPDI
EHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCE
IYHLGDVGKEPMILVFGRNPREVLEKIKMLI
EEEECCCCCCCEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]