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The map label for this gene is proS [H]

Identifier: 18977665

GI number: 18977665

Start: 1219937

End: 1221376

Strand: Direct

Name: proS [H]

Synonym: PF1293

Alternate gene names: 18977665

Gene position: 1219937-1221376 (Clockwise)

Preceding gene: 18977663

Following gene: 18977666

Centisome position: 63.93

GC content: 45.07

Gene sequence:

>1440_bases
ATGGTAGAGAGAAAGAAGTGGAGTGAAAACTTCAGTGAGTGGTTTAATGAAGTAATAGAGGAGGCTGGAATCCTAGACAA
AAGGTACCCTGTAAAAGGAATGAACGTCTGGCTTCCATATGGGCTTAAAATAATGAAGAACATTGAAAAATTCATTCATG
AAGAGATGGAGAGAACGGACCATCAGGAAGTTCTCTTTCCAGCTTTAATCCCTGAAACTGAGTTCCAAAAGGAGGCCGAG
CATATTGCTGGCTTTGAGGGAGAGGTTTTCTGGGTAACCCATGCTGGCCATGAGCCGTTGGATGTTAGATTAGTTTTGAG
GCCAACAAGTGAAACTGCAATGTACTCAATGTTTGCCCTCTGGATAAGGTCTCACGCTGATTTGCCTTTCAAGGTTTACC
AGATAGTCAACGTGTATAGGTATGAGACAAAGCACACTAGGCCTCTAATTAGAGTTAGGGAAATTAGCAGGTTCTTTGAG
GCCCACACAGCTCATGCAGACTTTGAAGATGCAGAGAGACAGATAAAGGAAGACCTTGAGATCTTTGATAACCTAATGAA
AAAGCTAGCAATAGCATATGTCATATCTAAAAGGCCAGAATGGGACAAGTTCCCAGGAGCTTACTACTCCCTGGGAGCTG
AAGTTGTAATGCCCGATGGAAGAACCTTGCAGATAGGAACGATGCACAACTATAAGCAGAACTTTGCCAAGGCATACAAC
ATCCTCTATGAGAAGGAGGACGGTACTCACGACTACGTTCACCAGACCACGTTTGGCATGAGTGAAAGATTGCTAGCTGC
AGTCATAGCAATTCACGGAGACGACAGGGGAATGGTTCTTCCACCAACCATAGCCCCAATCCAAGTTGTTATAGTTCCAA
TCCCAAAGAAGGGAAGTGAAGAGGAAGTTTACTCATATGCAAGAGAGATTGAGGAAGAGCTAAAGCTTGCTGGAATAAGG
GTGCACTTAGACTTGAGAGACAAGAGGCCTGGATGGAAGTTTTACGATTGGGAGCTTAAGGGGGTTCCAGTGAGAATTGA
AGTTGGGCCGAGGGATGCCGCTGAAAAGACCGTTGTTCTTGCAAGGAGGGACAAGCTCGAAAAGATAACGGTTTCAAGGG
AAGAGCTAGTTGATAAAGTTAGGGAGCTGTTTGATGACATAATGCAGTACCTCTACGAGAGGGCCAAAGAGTGGTTGGAG
AGCCACATCAAACGGGTTGACACAATTGAAGAGGCCAAGGAACTATTCAATGACAGGAGAGGTATAGTGGAGATTCCATG
GTGTGGAGAGGAGGAGTGTGGACTTAAAATGGAAGAGGAGCTGGAGGCTAAAATGCTTGGAATCCCTTATCCTGAGGAGA
GTGCAAGGGTGGAAGGTAAGAAGTGTCCCGTATGTGGTAGGGAAGCTAAATTCATAGCTAGGTTTGCAAGAACGTACTGA

Upstream 100 bases:

>100_bases
TGAATGCCTCACCATAACTTTCTTTCTGTCCATGGATATCGAGCGAAAATTAACAAAACTTATTTAAAAATAATCTCCCG
CTTTACCTTAGGTGGGTTGG

Downstream 100 bases:

>100_bases
GGGGGTTCCTCCCCCATGATACTTCTTTTGACAGGAATGCCAGGCTCGGGAAAGGGTGTTGTGGCTAGAGAGTTCGAAAA
AAGAGGAATACCTGTTGTTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MVERKKWSENFSEWFNEVIEEAGILDKRYPVKGMNVWLPYGLKIMKNIEKFIHEEMERTDHQEVLFPALIPETEFQKEAE
HIAGFEGEVFWVTHAGHEPLDVRLVLRPTSETAMYSMFALWIRSHADLPFKVYQIVNVYRYETKHTRPLIRVREISRFFE
AHTAHADFEDAERQIKEDLEIFDNLMKKLAIAYVISKRPEWDKFPGAYYSLGAEVVMPDGRTLQIGTMHNYKQNFAKAYN
ILYEKEDGTHDYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKGSEEEVYSYAREIEEELKLAGIR
VHLDLRDKRPGWKFYDWELKGVPVRIEVGPRDAAEKTVVLARRDKLEKITVSREELVDKVRELFDDIMQYLYERAKEWLE
SHIKRVDTIEEAKELFNDRRGIVEIPWCGEEECGLKMEEELEAKMLGIPYPEESARVEGKKCPVCGREAKFIARFARTY

Sequences:

>Translated_479_residues
MVERKKWSENFSEWFNEVIEEAGILDKRYPVKGMNVWLPYGLKIMKNIEKFIHEEMERTDHQEVLFPALIPETEFQKEAE
HIAGFEGEVFWVTHAGHEPLDVRLVLRPTSETAMYSMFALWIRSHADLPFKVYQIVNVYRYETKHTRPLIRVREISRFFE
AHTAHADFEDAERQIKEDLEIFDNLMKKLAIAYVISKRPEWDKFPGAYYSLGAEVVMPDGRTLQIGTMHNYKQNFAKAYN
ILYEKEDGTHDYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKGSEEEVYSYAREIEEELKLAGIR
VHLDLRDKRPGWKFYDWELKGVPVRIEVGPRDAAEKTVVLARRDKLEKITVSREELVDKVRELFDDIMQYLYERAKEWLE
SHIKRVDTIEEAKELFNDRRGIVEIPWCGEEECGLKMEEELEAKMLGIPYPEESARVEGKKCPVCGREAKFIARFARTY
>Mature_479_residues
MVERKKWSENFSEWFNEVIEEAGILDKRYPVKGMNVWLPYGLKIMKNIEKFIHEEMERTDHQEVLFPALIPETEFQKEAE
HIAGFEGEVFWVTHAGHEPLDVRLVLRPTSETAMYSMFALWIRSHADLPFKVYQIVNVYRYETKHTRPLIRVREISRFFE
AHTAHADFEDAERQIKEDLEIFDNLMKKLAIAYVISKRPEWDKFPGAYYSLGAEVVMPDGRTLQIGTMHNYKQNFAKAYN
ILYEKEDGTHDYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKGSEEEVYSYAREIEEELKLAGIR
VHLDLRDKRPGWKFYDWELKGVPVRIEVGPRDAAEKTVVLARRDKLEKITVSREELVDKVRELFDDIMQYLYERAKEWLE
SHIKRVDTIEEAKELFNDRRGIVEIPWCGEEECGLKMEEELEAKMLGIPYPEESARVEGKKCPVCGREAKFIARFARTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=495, Percent_Identity=35.959595959596, Blast_Score=308, Evalue=1e-83,
Organism=Escherichia coli, GI1788013, Length=391, Percent_Identity=23.0179028132992, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1786392, Length=169, Percent_Identity=31.3609467455621, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71991342, Length=489, Percent_Identity=34.9693251533742, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI71991346, Length=354, Percent_Identity=36.7231638418079, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321808, Length=499, Percent_Identity=33.6673346693387, Blast_Score=292, Evalue=9e-80,
Organism=Drosophila melanogaster, GI24649468, Length=495, Percent_Identity=33.7373737373737, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24649466, Length=495, Percent_Identity=33.7373737373737, Blast_Score=288, Evalue=5e-78,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 56149; Mature: 56149

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERKKWSENFSEWFNEVIEEAGILDKRYPVKGMNVWLPYGLKIMKNIEKFIHEEMERTD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCC
HQEVLFPALIPETEFQKEAEHIAGFEGEVFWVTHAGHEPLDVRLVLRPTSETAMYSMFAL
CHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHH
WIRSHADLPFKVYQIVNVYRYETKHTRPLIRVREISRFFEAHTAHADFEDAERQIKEDLE
HHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IFDNLMKKLAIAYVISKRPEWDKFPGAYYSLGAEVVMPDGRTLQIGTMHNYKQNFAKAYN
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHH
ILYEKEDGTHDYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKGSE
HHEECCCCCHHHHHHHHHCHHHHHHHHHHHEECCCCCEECCCCCCCEEEEEEECCCCCCH
EEVYSYAREIEEELKLAGIRVHLDLRDKRPGWKFYDWELKGVPVRIEVGPRDAAEKTVVL
HHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEEEEEECCEEEEEEECCCCCCHHEEEE
ARRDKLEKITVSREELVDKVRELFDDIMQYLYERAKEWLESHIKRVDTIEEAKELFNDRR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIVEIPWCGEEECGLKMEEELEAKMLGIPYPEESARVEGKKCPVCGREAKFIARFARTY
CEEECCCCCCHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVERKKWSENFSEWFNEVIEEAGILDKRYPVKGMNVWLPYGLKIMKNIEKFIHEEMERTD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCC
HQEVLFPALIPETEFQKEAEHIAGFEGEVFWVTHAGHEPLDVRLVLRPTSETAMYSMFAL
CHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHH
WIRSHADLPFKVYQIVNVYRYETKHTRPLIRVREISRFFEAHTAHADFEDAERQIKEDLE
HHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IFDNLMKKLAIAYVISKRPEWDKFPGAYYSLGAEVVMPDGRTLQIGTMHNYKQNFAKAYN
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHH
ILYEKEDGTHDYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKGSE
HHEECCCCCHHHHHHHHHCHHHHHHHHHHHEECCCCCEECCCCCCCEEEEEEECCCCCCH
EEVYSYAREIEEELKLAGIRVHLDLRDKRPGWKFYDWELKGVPVRIEVGPRDAAEKTVVL
HHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEEEEEECCEEEEEEECCCCCCHHEEEE
ARRDKLEKITVSREELVDKVRELFDDIMQYLYERAKEWLESHIKRVDTIEEAKELFNDRR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIVEIPWCGEEECGLKMEEELEAKMLGIPYPEESARVEGKKCPVCGREAKFIARFARTY
CEEECCCCCCHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]