Click here to switch to the map view.

The map label for this gene is kipI [H]

Identifier: 18977645

GI number: 18977645

Start: 1205343

End: 1206026

Strand: Direct

Name: kipI [H]

Synonym: PF1273

Alternate gene names: 18977645

Gene position: 1205343-1206026 (Clockwise)

Preceding gene: 18977644

Following gene: 18977646

Centisome position: 63.16

GC content: 40.94

Gene sequence:

>684_bases
ATGAAAGTTAGAGTGGTTGGGGACTCTTGCATTGCGATAATATTTGGCGAAGATATCAGCGAAGAAACAAACAGAAAAGT
TCATGCTTTGGCAGATTATTTGTTGAACTACAATCCTGAATGGCTTCTAGATGTTGTTCCCTCTTACTCGGCTCTTTATG
TTTACTTTGATCCCTTAATGATAGACTATCGAGAAGTTATAGACATCATAGGAAAAATCAAGATTAATGAAGTTGAAAGG
AAGGAAAAAAGAATCATTAAAATCCCCATAGCTTATGGAGGGGAATTCGGGCCAGACATAGAGTTCGTAGCTAAATACAA
TGGATTAAGTGTGGATGATGTAATTGAAATTCACTCAAAACCGCTTTATAGGGTTTATTTCCTTGGCTTTCTCCCAGGAT
TTGCTTACCTGGGTGGAATGGATGAGAGAATTGCTACACCGAGGCTTGAGAAGCCTCGCTTAAAAGTTCCTGCTGGAAGC
GTAGGAATTGCAGGAAAGCAGACTGGATGGTACGCTATAGAAAGCCCAGGGGGCTGGAGGATTATTGGAAGAACGCCCTT
GAGAACTTTTAATCCAGAAAAAATTCCCCCCAGCATCGTTTCTCCTGGAGATTATGTGAAATTCGTCCCAATAGATGAGA
ACGAATTCTGGGAAATTTACAGGGAGGAGTGGGGAAATGATTGA

Upstream 100 bases:

>100_bases
TCCACGGAGATAATCCAAAAGCAGTAGAGATTACCGCCTATATTAGAAGAAGACTAGAGGAGGAGGGAGTAAAGGTACTC
CCGATGGGGGATTTCATAAA

Downstream 100 bases:

>100_bases
AGTACTCTCTGTTATAACTTCTGCCACAGTTCAAGACCTGGGAAGGTGGGGATATCAGAGGTACGGTGTACCTGTAAGCG
GAGTTATGGATGAAGTTTCC

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKVRVVGDSCIAIIFGEDISEETNRKVHALADYLLNYNPEWLLDVVPSYSALYVYFDPLMIDYREVIDIIGKIKINEVER
KEKRIIKIPIAYGGEFGPDIEFVAKYNGLSVDDVIEIHSKPLYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGS
VGIAGKQTGWYAIESPGGWRIIGRTPLRTFNPEKIPPSIVSPGDYVKFVPIDENEFWEIYREEWGND

Sequences:

>Translated_227_residues
MKVRVVGDSCIAIIFGEDISEETNRKVHALADYLLNYNPEWLLDVVPSYSALYVYFDPLMIDYREVIDIIGKIKINEVER
KEKRIIKIPIAYGGEFGPDIEFVAKYNGLSVDDVIEIHSKPLYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGS
VGIAGKQTGWYAIESPGGWRIIGRTPLRTFNPEKIPPSIVSPGDYVKFVPIDENEFWEIYREEWGND
>Mature_227_residues
MKVRVVGDSCIAIIFGEDISEETNRKVHALADYLLNYNPEWLLDVVPSYSALYVYFDPLMIDYREVIDIIGKIKINEVER
KEKRIIKIPIAYGGEFGPDIEFVAKYNGLSVDDVIEIHSKPLYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGS
VGIAGKQTGWYAIESPGGWRIIGRTPLRTFNPEKIPPSIVSPGDYVKFVPIDENEFWEIYREEWGND

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=214, Percent_Identity=36.9158878504673, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 25910; Mature: 25910

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRVVGDSCIAIIFGEDISEETNRKVHALADYLLNYNPEWLLDVVPSYSALYVYFDPLM
CEEEEECCCEEEEEECCCCCHHHCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCHH
IDYREVIDIIGKIKINEVERKEKRIIKIPIAYGGEFGPDIEFVAKYNGLSVDDVIEIHSK
HHHHHHHHHHCCEEECHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHCCC
PLYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGSVGIAGKQTGWYAIESPGGWR
CHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCEE
IIGRTPLRTFNPEKIPPSIVSPGDYVKFVPIDENEFWEIYREEWGND
EEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKVRVVGDSCIAIIFGEDISEETNRKVHALADYLLNYNPEWLLDVVPSYSALYVYFDPLM
CEEEEECCCEEEEEECCCCCHHHCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCHH
IDYREVIDIIGKIKINEVERKEKRIIKIPIAYGGEFGPDIEFVAKYNGLSVDDVIEIHSK
HHHHHHHHHHCCEEECHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHCCC
PLYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGSVGIAGKQTGWYAIESPGGWR
CHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCEE
IIGRTPLRTFNPEKIPPSIVSPGDYVKFVPIDENEFWEIYREEWGND
EEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]